Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_017_K13
(402 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q15388|TOM20_HUMAN Mitochondrial import receptor subunit... 106 2e-23
sp|Q9DCC8|TOM20_MOUSE Mitochondrial import receptor subunit... 105 3e-23
sp|Q62760|TOM20_RAT Mitochondrial import receptor subunit T... 103 1e-22
sp|Q19766|TOM20_CAEEL Mitochondrial import receptor subunit... 77 2e-14
sp|P35848|TOM20_NEUCR Mitochondrial import receptor subunit... 34 0.12
sp|O14225|TOM20_SCHPO Mitochondrial import receptor subunit... 33 0.16
sp|Q6KAR6|EXOC3_MOUSE Exocyst complex component 3 (Exocyst ... 30 2.3
sp|Q62825|EXOC3_RAT Exocyst complex component 3 (Exocyst co... 30 2.3
sp|Q896M5|SYE_CLOTE Glutamyl-tRNA synthetase (Glutamate--tR... 28 5.2
sp|Q8VCF5|TSN10_MOUSE Tetraspanin-10 (Tspan-10) (Oculospanin) 28 6.8
>sp|Q15388|TOM20_HUMAN Mitochondrial import receptor subunit TOM20 homolog (Mitochondrial
20 kDa outer membrane protein) (Outer mitochondrial
membrane receptor Tom20)
Length = 145
Score = 106 bits (264), Expect = 2e-23
Identities = 53/127 (41%), Positives = 78/127 (61%), Gaps = 3/127 (2%)
Frame = +1
Query: 19 ALGAGVA---FISYCIYFDRKRRSHPDFXXXXXXXXXXXXDNKMKIKIPTLPDMNNNVAR 189
A+ AGV FI YCIYFDRKRRS P+F K + + LPD+ + A
Sbjct: 7 AIAAGVCGALFIGYCIYFDRKRRSDPNFKNRLRERRKKQKLAKERAGLSKLPDLKDAEAV 66
Query: 190 QEFFLEQIRYGDQCMSTGAFEEGIQRLALALSVCANPQNLLKVFEQTLQPQAYDMLLTTL 369
Q+FFLE+I+ G++ ++ G +E+G+ L A++VC PQ LL+V +QTL P + MLLT L
Sbjct: 67 QKFFLEEIQLGEELLAQGEYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKL 126
Query: 370 PKMKEQV 390
P + +++
Sbjct: 127 PTISQRI 133
>sp|Q9DCC8|TOM20_MOUSE Mitochondrial import receptor subunit TOM20 homolog (Mitochondrial
20 kDa outer membrane protein) (Outer mitochondrial
membrane receptor Tom20)
Length = 145
Score = 105 bits (263), Expect = 3e-23
Identities = 53/127 (41%), Positives = 78/127 (61%), Gaps = 3/127 (2%)
Frame = +1
Query: 19 ALGAGVA---FISYCIYFDRKRRSHPDFXXXXXXXXXXXXDNKMKIKIPTLPDMNNNVAR 189
A+ AGV FI YCIYFDRKRRS P+F K + + LPD+ + A
Sbjct: 7 AIAAGVCGALFIGYCIYFDRKRRSDPNFKNRLRERRKKQKLAKERAGLSKLPDLKDAEAV 66
Query: 190 QEFFLEQIRYGDQCMSTGAFEEGIQRLALALSVCANPQNLLKVFEQTLQPQAYDMLLTTL 369
Q+FFLE+I+ G++ ++ G +E+G+ L A++VC PQ LL+V +QTL P + MLLT L
Sbjct: 67 QKFFLEEIQLGEELLAQGDYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKL 126
Query: 370 PKMKEQV 390
P + +++
Sbjct: 127 PTISQRI 133
>sp|Q62760|TOM20_RAT Mitochondrial import receptor subunit TOM20 homolog (Mitochondrial
20 kDa outer membrane protein) (Outer mitochondrial
membrane receptor Tom20)
Length = 145
Score = 103 bits (258), Expect = 1e-22
Identities = 49/120 (40%), Positives = 73/120 (60%)
Frame = +1
Query: 31 GVAFISYCIYFDRKRRSHPDFXXXXXXXXXXXXDNKMKIKIPTLPDMNNNVARQEFFLEQ 210
G FI YCIYFDRKRR P+F K + + LPD+ + A Q+FFLE+
Sbjct: 14 GALFIGYCIYFDRKRRGDPNFKNRLRERRKKQKLAKERAGLSKLPDLKDAEAVQKFFLEE 73
Query: 211 IRYGDQCMSTGAFEEGIQRLALALSVCANPQNLLKVFEQTLQPQAYDMLLTTLPKMKEQV 390
I+ G++ ++ G +E+G+ L A++VC PQ LL+V +QTL P + MLLT LP + +++
Sbjct: 74 IQLGEELLAQGDYEKGVDHLTNAIAVCGQPQQLLQVLQQTLPPPVFQMLLTKLPTISQRI 133
>sp|Q19766|TOM20_CAEEL Mitochondrial import receptor subunit TOM20 homolog (Mitochondrial
20 kDa outer membrane protein) (Outer mitochondrial
membrane receptor TOM20)
Length = 188
Score = 76.6 bits (187), Expect = 2e-14
Identities = 43/137 (31%), Positives = 71/137 (51%), Gaps = 8/137 (5%)
Frame = +1
Query: 13 IVALG-AGVAFISYCIYFDRKRRSHPDFXXXXXXXXXXXXD-NKMKIKIPTL------PD 168
++A G AG AF+ YCIYFD KR + PD+ M + P PD
Sbjct: 14 VLAAGIAGAAFLGYCIYFDHKRINAPDYKDKIRQKRRAQAGAGGMAPRRPAAAGNDAAPD 73
Query: 169 MNNNVARQEFFLEQIRYGDQCMSTGAFEEGIQRLALALSVCANPQNLLKVFEQTLQPQAY 348
+ + Q FFL++++ G++ M+ G +EG +A A+ +C Q LL +F+QTL +
Sbjct: 74 VTDPSQMQRFFLQEVQLGEELMAAGNVDEGAVHIANAVMLCGESQQLLSIFQQTLSEDQF 133
Query: 349 DMLLTTLPKMKEQVDEL 399
++ LP +E++ E+
Sbjct: 134 RAVVQQLPSTRERLAEM 150
>sp|P35848|TOM20_NEUCR Mitochondrial import receptor subunit tom-20 (Mitochondrial 20 kDa
outer membrane protein) (Translocase of outer membrane
20 kDa subunit) (MOM19 protein)
Length = 181
Score = 33.9 bits (76), Expect = 0.12
Identities = 34/142 (23%), Positives = 56/142 (39%), Gaps = 26/142 (18%)
Frame = +1
Query: 10 YIVALGAGVA--FISYCIYFDRKRRSHPDF----------------------XXXXXXXX 117
Y A A VA F++Y +YFD KRR+ P+F
Sbjct: 8 YTTAAVAAVATGFLAYAVYFDYKRRNDPEFRRQLRRSARRQARQEKEYAELSQQAQRQRI 67
Query: 118 XXXXDNKMKIKIPTLPDMNNNVARQEFFLEQIRYGDQCMS--TGAFEEGIQRLALALSVC 291
D + PT D ++ +FLEQ++ G+ T A + + AL V
Sbjct: 68 RQMVDEAKEEGFPTTSD-----EKEAYFLEQVQAGEILGQDPTKAIDASL-AFYKALKVY 121
Query: 292 ANPQNLLKVFEQTLQPQAYDML 357
P +L+ ++++T+ D+L
Sbjct: 122 PTPGDLISIYDKTVAKPILDIL 143
>sp|O14225|TOM20_SCHPO Mitochondrial import receptor subunit tom20 (Mitochondrial 20 kDa
outer membrane protein) (Translocase of outer membrane
20 kDa subunit)
Length = 152
Score = 33.5 bits (75), Expect = 0.16
Identities = 27/126 (21%), Positives = 49/126 (38%), Gaps = 19/126 (15%)
Frame = +1
Query: 37 AFISYCIYFDRKRRSHPDFXXXXXXXXXXXXDNKMKIK-----------------IPTLP 165
A + Y IYFD KRR+ P F + K + + + + P
Sbjct: 14 AAVGYAIYFDYKRRNDPHFRKTLKRRYKKVHEAKKQEEKLATKKFDITVEEALQVVASTP 73
Query: 166 DMNNNVARQEFFLEQIRYGDQC--MSTGAFEEGIQRLALALSVCANPQNLLKVFEQTLQP 339
++ ++ FF++Q+ G+Q +E AL V P L ++E+T+
Sbjct: 74 VPSSAEEKELFFMQQVARGEQLFQQQPDNIKESAACFYSALKVYPQPVELFAIYERTVPE 133
Query: 340 QAYDML 357
++L
Sbjct: 134 PIMNLL 139
>sp|Q6KAR6|EXOC3_MOUSE Exocyst complex component 3 (Exocyst complex component Sec6)
Length = 755
Score = 29.6 bits (65), Expect = 2.3
Identities = 19/60 (31%), Positives = 29/60 (48%)
Frame = -3
Query: 352 YRMPEVVKFVQKL*EDFEDLHIHSKLKLVFEYLLQKHQLTCIDHRNESVPKRILVLLHYY 173
+ +PE+V+ Q L E L H KL + + L C +R +S KR + L+H Y
Sbjct: 130 FSVPEIVRETQDLIEQGALLQAHRKL---MDLECSRDGLMCEQYRMDSGNKRDMTLIHGY 186
>sp|Q62825|EXOC3_RAT Exocyst complex component 3 (Exocyst complex component Sec6)
(rSec6)
Length = 755
Score = 29.6 bits (65), Expect = 2.3
Identities = 19/60 (31%), Positives = 29/60 (48%)
Frame = -3
Query: 352 YRMPEVVKFVQKL*EDFEDLHIHSKLKLVFEYLLQKHQLTCIDHRNESVPKRILVLLHYY 173
+ +PE+V+ Q L E L H KL + + L C +R +S KR + L+H Y
Sbjct: 130 FSVPEIVRETQDLIEQGALLQAHRKL---MDLECSRDGLMCEQYRMDSGNKRDMTLIHGY 186
>sp|Q896M5|SYE_CLOTE Glutamyl-tRNA synthetase (Glutamate--tRNA ligase) (GluRS)
Length = 488
Score = 28.5 bits (62), Expect = 5.2
Identities = 21/61 (34%), Positives = 33/61 (54%), Gaps = 6/61 (9%)
Frame = -3
Query: 346 MPEVVKFVQKL*EDFEDLHIHSKLKLVFEYLLQKHQ-----LTCIDHRN-ESVPKRILVL 185
+PE V F +L E DL++H K+K FE L+ + L ID+ N E++ I+ L
Sbjct: 371 IPEQVDFFDELPEYSCDLYVHKKMKTNFENSLESLEKALPVLESIDNWNTETIHDSIMEL 430
Query: 184 L 182
+
Sbjct: 431 I 431
>sp|Q8VCF5|TSN10_MOUSE Tetraspanin-10 (Tspan-10) (Oculospanin)
Length = 331
Score = 28.1 bits (61), Expect = 6.8
Identities = 14/48 (29%), Positives = 23/48 (47%), Gaps = 12/48 (25%)
Frame = +1
Query: 196 FFLEQIRYGDQCMSTGAFEE------------GIQRLALALSVCANPQ 303
F L+Q++ G QC ++++ G+Q +L S C NPQ
Sbjct: 199 FLLDQVQLGLQCCGAVSYQDWQQNLYFNCSSPGVQACSLPASCCINPQ 246
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 35,012,886
Number of Sequences: 369166
Number of extensions: 494380
Number of successful extensions: 1269
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1239
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1265
length of database: 68,354,980
effective HSP length: 98
effective length of database: 50,250,950
effective search space used: 1758783250
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)