Planaria EST Database


DrC_02204

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_02204
         (592 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8BU30|SYI_MOUSE  Isoleucyl-tRNA synthetase, cytoplasmic ...    64   4e-10
sp|P41252|SYI_HUMAN  Isoleucyl-tRNA synthetase, cytoplasmic ...    63   5e-10
sp|P09436|SYIC_YEAST  Isoleucyl-tRNA synthetase, cytoplasmic...    50   6e-06
sp|O13651|SYIC_SCHPO  Isoleucyl-tRNA synthetase, cytoplasmic...    49   9e-06
sp|Q21926|SYI_CAEEL  Isoleucyl-tRNA synthetase, cytoplasmic ...    42   0.001
sp|Q9Z972|SYI_CHLPN  Isoleucyl-tRNA synthetase (Isoleucine--...    42   0.001
sp|P41368|SYIP_STAAU  Isoleucyl-tRNA synthetase, mupirocin r...    40   0.006
sp|Q9YF67|SYI_AERPE  Isoleucyl-tRNA synthetase (Isoleucine--...    39   0.013
sp|Q58357|SYI_METJA  Isoleucyl-tRNA synthetase (Isoleucine--...    38   0.022
sp|Q9V072|SYI_PYRAB  Isoleucyl-tRNA synthetase (Isoleucine--...    37   0.037
>sp|Q8BU30|SYI_MOUSE Isoleucyl-tRNA synthetase, cytoplasmic (Isoleucine--tRNA ligase)
            (IleRS) (IRS)
          Length = 1262

 Score = 63.5 bits (153), Expect = 4e-10
 Identities = 54/189 (28%), Positives = 86/189 (45%), Gaps = 11/189 (5%)
 Frame = +2

Query: 32   QYESRSTPDGILVLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNIY--CWTE 205
            Q+E+ S     LVL+D    + +  EG+ARE+INRIQ+ RK+ NL+P D + +Y    +E
Sbjct: 960  QFEAHSDAQA-LVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYNAKSE 1018

Query: 206  NEDVRSVFTSHADMIHRTILQPIIVKDVSNKADILGECSILSDISGTVCEIKESPLKMWF 385
               + +V  SH D +  TI  P+    V    +IL +            ++K S L++  
Sbjct: 1019 GRYLNNVIESHTDFVFATIKAPLKPYPVPTSDNILIQ---------EQTQLKGSELEITL 1069

Query: 386  THAHDRIETGNRKCNPPKTCTEI---IC---EELGSTIQIDATKIE---SWDQFRRVVRS 538
            T            C P   C  +   IC    E G  + ++  K +   +  + + VV S
Sbjct: 1070 TKG---------SCVPGPACAYVNLNICANGTEQGGVLLLENPKGDNQLNLVKLKTVVTS 1120

Query: 539  AFGWTNSAL 565
             FG  N+ L
Sbjct: 1121 VFGVKNAKL 1129
>sp|P41252|SYI_HUMAN Isoleucyl-tRNA synthetase, cytoplasmic (Isoleucine--tRNA ligase)
            (IleRS) (IRS)
          Length = 1266

 Score = 63.2 bits (152), Expect = 5e-10
 Identities = 36/95 (37%), Positives = 54/95 (56%), Gaps = 2/95 (2%)
 Frame = +2

Query: 32   QYESRSTPDGILVLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNIYCWTENE 211
            Q+E+ S     LVL+D    + +  EG+ARE+INRIQ+ RK+ NL+P D + +Y   ++E
Sbjct: 964  QFEAHSDAQA-LVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKAKSE 1022

Query: 212  D--VRSVFTSHADMIHRTILQPIIVKDVSNKADIL 310
               + SV  SH + I  TI  P+    VS    +L
Sbjct: 1023 GTYLNSVIESHTEFIFTTIKAPLKPYPVSPSDKVL 1057
>sp|P09436|SYIC_YEAST Isoleucyl-tRNA synthetase, cytoplasmic (Isoleucine--tRNA ligase)
            (IleRS)
          Length = 1072

 Score = 49.7 bits (117), Expect = 6e-06
 Identities = 34/110 (30%), Positives = 59/110 (53%), Gaps = 2/110 (1%)
 Frame = +2

Query: 38   ESRSTPDGILVLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNI--YCWTENE 211
            E+R+  D +L++MD +   +L  EG+ARE++NRIQ+ RK+  L   D V +      +  
Sbjct: 963  ETRTDQD-VLIIMDTNIYSELKSEGLARELVNRIQKLRKKCGLEATDDVLVEYELVKDTI 1021

Query: 212  DVRSVFTSHADMIHRTILQPIIVKDVSNKADILGECSILSDISGTVCEIK 361
            D  ++   H DM+ +T    I   D  +K D +G+      I+ T+ ++K
Sbjct: 1022 DFEAIVKEHFDMLSKTCRSDIAKYD-GSKTDPIGDEE--QSINDTIFKLK 1068
>sp|O13651|SYIC_SCHPO Isoleucyl-tRNA synthetase, cytoplasmic (Isoleucine--tRNA ligase)
            (IleRS)
          Length = 1064

 Score = 48.9 bits (115), Expect = 9e-06
 Identities = 30/92 (32%), Positives = 53/92 (57%), Gaps = 3/92 (3%)
 Frame = +2

Query: 44   RSTPDGI-LVLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNIYCWTENEDV- 217
            +S  DGI +VL+D      L  EG+ARE+INR+QR RK+ NL   D V +    +N+ + 
Sbjct: 954  KSNTDGICIVLLDIEIDAQLQAEGLAREVINRVQRLRKKSNLQVTDDVRMTYKIKNDTIG 1013

Query: 218  -RSVFTSHADMIHRTILQPIIVKDVSNKADIL 310
              S   S+  +  + + +PI  +  +++++I+
Sbjct: 1014 LESAVDSNEALFSKVLRRPIEKETGADESNII 1045
>sp|Q21926|SYI_CAEEL Isoleucyl-tRNA synthetase, cytoplasmic (Isoleucine--tRNA ligase)
            (IleRS)
          Length = 1141

 Score = 42.0 bits (97), Expect = 0.001
 Identities = 16/43 (37%), Positives = 29/43 (67%)
 Frame = +2

Query: 65   LVLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNIY 193
            +V++D  + E L +EG+ RE+ NR+QR RK+  L+  D  +++
Sbjct: 962  IVMIDTTEDESLIEEGLCREVTNRVQRLRKQAKLVSTDTAHVH 1004
>sp|Q9Z972|SYI_CHLPN Isoleucyl-tRNA synthetase (Isoleucine--tRNA ligase) (IleRS)
          Length = 1043

 Score = 42.0 bits (97), Expect = 0.001
 Identities = 32/98 (32%), Positives = 47/98 (47%)
 Frame = +2

Query: 68   VLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNIYCWTENEDVRSVFTSHADM 247
            V++DC   E L  EGIARE++N+I   R+   L   D + +   T  E V   F  + + 
Sbjct: 944  VILDCQLREPLIVEGIARELVNKINTMRRNQQLHVSDRIALRIKT-TEAVHRAFLDYENY 1002

Query: 248  IHRTILQPIIVKDVSNKADILGECSILSDISGTVCEIK 361
            I    L  II  D +  +D  GE     DI+G   +I+
Sbjct: 1003 ICEETL--IIAYDFTQDSDFQGE---NWDINGHATQIE 1035
>sp|P41368|SYIP_STAAU Isoleucyl-tRNA synthetase, mupirocin resistant (Isoleucine--tRNA
            ligase) (IleRS) (Mupirocin resistance protein)
          Length = 1024

 Score = 39.7 bits (91), Expect = 0.006
 Identities = 22/63 (34%), Positives = 37/63 (58%)
 Frame = +2

Query: 47   STPDGILVLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNIYCWTENEDVRSV 226
            S  +  ++L+D + S +L +EG ARE+I  IQ+ RK+ NL     ++IY     E + S+
Sbjct: 924  SEDNDCVILLDKNLSPELIREGHARELIRLIQQLRKKKNLPINQRIDIYIGVTGELLESI 983

Query: 227  FTS 235
             T+
Sbjct: 984  KTN 986
>sp|Q9YF67|SYI_AERPE Isoleucyl-tRNA synthetase (Isoleucine--tRNA ligase) (IleRS)
          Length = 1064

 Score = 38.5 bits (88), Expect = 0.013
 Identities = 27/72 (37%), Positives = 38/72 (52%), Gaps = 1/72 (1%)
 Frame = +2

Query: 44   RSTPDGILVLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNIYCWTE-NEDVR 220
            R TP G LV +D   +++   EG+AR+I+ RIQ  RKE     ED V +  W +  EDV 
Sbjct: 956  RDTPLG-LVAVDTRLTKEEVLEGLARDIVRRIQAMRKEAGYSVEDYVEV--WIQGGEDVV 1012

Query: 221  SVFTSHADMIHR 256
                +  + I R
Sbjct: 1013 EAVNALKEYIKR 1024
>sp|Q58357|SYI_METJA Isoleucyl-tRNA synthetase (Isoleucine--tRNA ligase) (IleRS)
          Length = 1039

 Score = 37.7 bits (86), Expect = 0.022
 Identities = 17/41 (41%), Positives = 28/41 (68%)
 Frame = +2

Query: 68   VLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNI 190
            V ++   ++DL KEG+ RE+I RIQ  RK+++L  E+ + I
Sbjct: 944  VFINTEITDDLIKEGLMREVIRRIQAMRKDMDLDIEEKIKI 984
>sp|Q9V072|SYI_PYRAB Isoleucyl-tRNA synthetase (Isoleucine--tRNA ligase) (IleRS)
          Length = 1067

 Score = 37.0 bits (84), Expect = 0.037
 Identities = 20/54 (37%), Positives = 31/54 (57%)
 Frame = +2

Query: 56   DGILVLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNIYCWTENEDV 217
            +G  V +D   + +L  EG+ARE + RIQ  RK L+L   D + +   T +E+V
Sbjct: 969  EGGRVFVDKTLTRELIAEGLAREFVRRIQEMRKRLDLDVNDRIIVTIETTDENV 1022
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69,726,869
Number of Sequences: 369166
Number of extensions: 1356059
Number of successful extensions: 3671
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3593
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3671
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4455160255
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)