Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_016_H20
(592 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8BU30|SYI_MOUSE Isoleucyl-tRNA synthetase, cytoplasmic ... 64 4e-10
sp|P41252|SYI_HUMAN Isoleucyl-tRNA synthetase, cytoplasmic ... 63 5e-10
sp|P09436|SYIC_YEAST Isoleucyl-tRNA synthetase, cytoplasmic... 50 6e-06
sp|O13651|SYIC_SCHPO Isoleucyl-tRNA synthetase, cytoplasmic... 49 9e-06
sp|Q21926|SYI_CAEEL Isoleucyl-tRNA synthetase, cytoplasmic ... 42 0.001
sp|Q9Z972|SYI_CHLPN Isoleucyl-tRNA synthetase (Isoleucine--... 42 0.001
sp|P41368|SYIP_STAAU Isoleucyl-tRNA synthetase, mupirocin r... 40 0.006
sp|Q9YF67|SYI_AERPE Isoleucyl-tRNA synthetase (Isoleucine--... 39 0.013
sp|Q58357|SYI_METJA Isoleucyl-tRNA synthetase (Isoleucine--... 38 0.022
sp|Q9V072|SYI_PYRAB Isoleucyl-tRNA synthetase (Isoleucine--... 37 0.037
>sp|Q8BU30|SYI_MOUSE Isoleucyl-tRNA synthetase, cytoplasmic (Isoleucine--tRNA ligase)
(IleRS) (IRS)
Length = 1262
Score = 63.5 bits (153), Expect = 4e-10
Identities = 54/189 (28%), Positives = 86/189 (45%), Gaps = 11/189 (5%)
Frame = +2
Query: 32 QYESRSTPDGILVLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNIY--CWTE 205
Q+E+ S LVL+D + + EG+ARE+INRIQ+ RK+ NL+P D + +Y +E
Sbjct: 960 QFEAHSDAQA-LVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYNAKSE 1018
Query: 206 NEDVRSVFTSHADMIHRTILQPIIVKDVSNKADILGECSILSDISGTVCEIKESPLKMWF 385
+ +V SH D + TI P+ V +IL + ++K S L++
Sbjct: 1019 GRYLNNVIESHTDFVFATIKAPLKPYPVPTSDNILIQ---------EQTQLKGSELEITL 1069
Query: 386 THAHDRIETGNRKCNPPKTCTEI---IC---EELGSTIQIDATKIE---SWDQFRRVVRS 538
T C P C + IC E G + ++ K + + + + VV S
Sbjct: 1070 TKG---------SCVPGPACAYVNLNICANGTEQGGVLLLENPKGDNQLNLVKLKTVVTS 1120
Query: 539 AFGWTNSAL 565
FG N+ L
Sbjct: 1121 VFGVKNAKL 1129
>sp|P41252|SYI_HUMAN Isoleucyl-tRNA synthetase, cytoplasmic (Isoleucine--tRNA ligase)
(IleRS) (IRS)
Length = 1266
Score = 63.2 bits (152), Expect = 5e-10
Identities = 36/95 (37%), Positives = 54/95 (56%), Gaps = 2/95 (2%)
Frame = +2
Query: 32 QYESRSTPDGILVLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNIYCWTENE 211
Q+E+ S LVL+D + + EG+ARE+INRIQ+ RK+ NL+P D + +Y ++E
Sbjct: 964 QFEAHSDAQA-LVLLDVTPDQSMVDEGMAREVINRIQKLRKKCNLVPTDEITVYYKAKSE 1022
Query: 212 D--VRSVFTSHADMIHRTILQPIIVKDVSNKADIL 310
+ SV SH + I TI P+ VS +L
Sbjct: 1023 GTYLNSVIESHTEFIFTTIKAPLKPYPVSPSDKVL 1057
>sp|P09436|SYIC_YEAST Isoleucyl-tRNA synthetase, cytoplasmic (Isoleucine--tRNA ligase)
(IleRS)
Length = 1072
Score = 49.7 bits (117), Expect = 6e-06
Identities = 34/110 (30%), Positives = 59/110 (53%), Gaps = 2/110 (1%)
Frame = +2
Query: 38 ESRSTPDGILVLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNI--YCWTENE 211
E+R+ D +L++MD + +L EG+ARE++NRIQ+ RK+ L D V + +
Sbjct: 963 ETRTDQD-VLIIMDTNIYSELKSEGLARELVNRIQKLRKKCGLEATDDVLVEYELVKDTI 1021
Query: 212 DVRSVFTSHADMIHRTILQPIIVKDVSNKADILGECSILSDISGTVCEIK 361
D ++ H DM+ +T I D +K D +G+ I+ T+ ++K
Sbjct: 1022 DFEAIVKEHFDMLSKTCRSDIAKYD-GSKTDPIGDEE--QSINDTIFKLK 1068
>sp|O13651|SYIC_SCHPO Isoleucyl-tRNA synthetase, cytoplasmic (Isoleucine--tRNA ligase)
(IleRS)
Length = 1064
Score = 48.9 bits (115), Expect = 9e-06
Identities = 30/92 (32%), Positives = 53/92 (57%), Gaps = 3/92 (3%)
Frame = +2
Query: 44 RSTPDGI-LVLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNIYCWTENEDV- 217
+S DGI +VL+D L EG+ARE+INR+QR RK+ NL D V + +N+ +
Sbjct: 954 KSNTDGICIVLLDIEIDAQLQAEGLAREVINRVQRLRKKSNLQVTDDVRMTYKIKNDTIG 1013
Query: 218 -RSVFTSHADMIHRTILQPIIVKDVSNKADIL 310
S S+ + + + +PI + +++++I+
Sbjct: 1014 LESAVDSNEALFSKVLRRPIEKETGADESNII 1045
>sp|Q21926|SYI_CAEEL Isoleucyl-tRNA synthetase, cytoplasmic (Isoleucine--tRNA ligase)
(IleRS)
Length = 1141
Score = 42.0 bits (97), Expect = 0.001
Identities = 16/43 (37%), Positives = 29/43 (67%)
Frame = +2
Query: 65 LVLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNIY 193
+V++D + E L +EG+ RE+ NR+QR RK+ L+ D +++
Sbjct: 962 IVMIDTTEDESLIEEGLCREVTNRVQRLRKQAKLVSTDTAHVH 1004
>sp|Q9Z972|SYI_CHLPN Isoleucyl-tRNA synthetase (Isoleucine--tRNA ligase) (IleRS)
Length = 1043
Score = 42.0 bits (97), Expect = 0.001
Identities = 32/98 (32%), Positives = 47/98 (47%)
Frame = +2
Query: 68 VLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNIYCWTENEDVRSVFTSHADM 247
V++DC E L EGIARE++N+I R+ L D + + T E V F + +
Sbjct: 944 VILDCQLREPLIVEGIARELVNKINTMRRNQQLHVSDRIALRIKT-TEAVHRAFLDYENY 1002
Query: 248 IHRTILQPIIVKDVSNKADILGECSILSDISGTVCEIK 361
I L II D + +D GE DI+G +I+
Sbjct: 1003 ICEETL--IIAYDFTQDSDFQGE---NWDINGHATQIE 1035
>sp|P41368|SYIP_STAAU Isoleucyl-tRNA synthetase, mupirocin resistant (Isoleucine--tRNA
ligase) (IleRS) (Mupirocin resistance protein)
Length = 1024
Score = 39.7 bits (91), Expect = 0.006
Identities = 22/63 (34%), Positives = 37/63 (58%)
Frame = +2
Query: 47 STPDGILVLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNIYCWTENEDVRSV 226
S + ++L+D + S +L +EG ARE+I IQ+ RK+ NL ++IY E + S+
Sbjct: 924 SEDNDCVILLDKNLSPELIREGHARELIRLIQQLRKKKNLPINQRIDIYIGVTGELLESI 983
Query: 227 FTS 235
T+
Sbjct: 984 KTN 986
>sp|Q9YF67|SYI_AERPE Isoleucyl-tRNA synthetase (Isoleucine--tRNA ligase) (IleRS)
Length = 1064
Score = 38.5 bits (88), Expect = 0.013
Identities = 27/72 (37%), Positives = 38/72 (52%), Gaps = 1/72 (1%)
Frame = +2
Query: 44 RSTPDGILVLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNIYCWTE-NEDVR 220
R TP G LV +D +++ EG+AR+I+ RIQ RKE ED V + W + EDV
Sbjct: 956 RDTPLG-LVAVDTRLTKEEVLEGLARDIVRRIQAMRKEAGYSVEDYVEV--WIQGGEDVV 1012
Query: 221 SVFTSHADMIHR 256
+ + I R
Sbjct: 1013 EAVNALKEYIKR 1024
>sp|Q58357|SYI_METJA Isoleucyl-tRNA synthetase (Isoleucine--tRNA ligase) (IleRS)
Length = 1039
Score = 37.7 bits (86), Expect = 0.022
Identities = 17/41 (41%), Positives = 28/41 (68%)
Frame = +2
Query: 68 VLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNI 190
V ++ ++DL KEG+ RE+I RIQ RK+++L E+ + I
Sbjct: 944 VFINTEITDDLIKEGLMREVIRRIQAMRKDMDLDIEEKIKI 984
>sp|Q9V072|SYI_PYRAB Isoleucyl-tRNA synthetase (Isoleucine--tRNA ligase) (IleRS)
Length = 1067
Score = 37.0 bits (84), Expect = 0.037
Identities = 20/54 (37%), Positives = 31/54 (57%)
Frame = +2
Query: 56 DGILVLMDCHQSEDLAKEGIAREIINRIQRRRKELNLIPEDPVNIYCWTENEDV 217
+G V +D + +L EG+ARE + RIQ RK L+L D + + T +E+V
Sbjct: 969 EGGRVFVDKTLTRELIAEGLAREFVRRIQEMRKRLDLDVNDRIIVTIETTDENV 1022
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69,726,869
Number of Sequences: 369166
Number of extensions: 1356059
Number of successful extensions: 3671
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3593
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3671
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4455160255
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)