Planaria EST Database


DrC_02051

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_02051
         (843 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P59679|ARP8_BRARE  Actin-related protein 8                      67   5e-11
sp|Q9H981|ARP8_HUMAN  Actin-related protein 8                      61   4e-09
sp|Q8R2S9|ARP8_MOUSE  Actin-related protein 8                      60   6e-09
sp|Q9US07|ARP8_SCHPO  Probable actin-related protein 8             52   2e-06
sp|Q9VX09|ARP8_DROME  Probable actin-related protein 8             42   0.002
sp|Q12386|ARP8_YEAST  Actin-like protein ARP8                      37   0.052
sp|P34529|DCR1_CAEEL  Endoribonuclease dcr-1                       33   0.76 
sp|Q89B04|PT1_BUCBP  Phosphoenolpyruvate-protein phosphotran...    33   0.99 
sp|Q9PQH1|IF2_UREPA  Translation initiation factor IF-2            32   1.7  
sp|P42928|SPI1_RABPU  Serine proteinase inhibitor 1 (Serpin ...    31   3.7  
>sp|P59679|ARP8_BRARE Actin-related protein 8
          Length = 623

 Score = 67.4 bits (163), Expect = 5e-11
 Identities = 38/93 (40%), Positives = 55/93 (59%)
 Frame = +3

Query: 552 SKTAAVKDLEDIWRTGLEKLLKIPSKDLSNYRVILVIPDLVIRCEIRWITEMLIYQFGI* 731
           S +A + DLE IW   L+KLL+IP KDL  YR IL+IPD+  R  ++ I  ML+ + G  
Sbjct: 200 SLSAVMMDLEHIWTHALQKLLQIPLKDLKYYRCILLIPDIYNRQHVKEIVNMLLVKMGF- 258

Query: 732 *YRVTTRECLCYIQAPGLPTACVVNMHENSISI 830
              V  +E +C     GL +ACVV++ +   S+
Sbjct: 259 SAVVVHQESVCATFGSGLSSACVVDVGDQKTSV 291

 Score = 47.4 bits (111), Expect = 5e-05
 Identities = 36/139 (25%), Positives = 66/139 (47%), Gaps = 4/139 (2%)
 Frame = +2

Query: 110 IIVIHPSSTKLRFGRSNDSEPIIIPHCIARKSKK--LSKVVRQPLIPDNRTCTYINTKFN 283
           I+VIHP S  LR GR+ D+ PI +PH IAR+ K    S+   + L+ +       N +  
Sbjct: 48  IVVIHPGSRTLRLGRATDTLPISVPHVIARRHKHPGQSRYEDKCLLREGLNSADSNEQRQ 107

Query: 284 NAIKLASIAMKSSSVEALQPYKEQPTINELIQMNKQYLKLESLLDMQD--NDIQRVYFEE 457
           N +K+    + S  +      +  P   E  ++  + ++  ++LD     +     +  E
Sbjct: 108 NGLKMVDQVIWSKKMS--NGVRRTPVSAEQARLYNRQIR-PAVLDPNSKVSWTNTSHHPE 164

Query: 458 FYIGKEALDIPIDANYNIH 514
           + +G+EAL +     Y++H
Sbjct: 165 YVVGEEALYVNPTDCYSVH 183
>sp|Q9H981|ARP8_HUMAN Actin-related protein 8
          Length = 624

 Score = 60.8 bits (146), Expect = 4e-09
 Identities = 31/93 (33%), Positives = 53/93 (56%)
 Frame = +3

Query: 552 SKTAAVKDLEDIWRTGLEKLLKIPSKDLSNYRVILVIPDLVIRCEIRWITEMLIYQFGI* 731
           S TA + D+E IW   ++K L+IP KDL  YR IL+IPD+  +  ++ +  M++ + G  
Sbjct: 200 SLTAVLADIEVIWSHAIQKYLEIPLKDLKYYRCILLIPDIYNKQHVKELVNMILMKMGFS 259

Query: 732 *YRVTTRECLCYIQAPGLPTACVVNMHENSISI 830
              V  +E +C     GL + C+V++ +   S+
Sbjct: 260 GI-VVHQESVCATYGSGLSSTCIVDVGDQKTSV 291

 Score = 51.6 bits (122), Expect = 3e-06
 Identities = 42/139 (30%), Positives = 67/139 (48%), Gaps = 4/139 (2%)
 Frame = +2

Query: 110 IIVIHPSSTKLRFGRSNDSEPIIIPHCIARKSKKLSKVVRQP--LIPDNRTCTYINTKFN 283
           IIVIHP ST LR GR+ D+ P  IPH IAR+ K+  + + +   L+ +       N +  
Sbjct: 48  IIVIHPGSTTLRIGRATDTLPASIPHVIARRHKQQGQPLYKDSWLLREGLNKPESNEQRQ 107

Query: 284 NAIKLASIAMKSSSVEALQPYKEQPTINELIQMNKQYLKLESLLDMQDND-IQRVYFEEF 460
           N +K+   A+ S  +      +  P   E  +   + ++   L     N      +  E+
Sbjct: 108 NGLKMVDQAIWSKKMS--NGTRRIPVSPEQARSYNKQMRPAILDHCSGNKWTNTSHHPEY 165

Query: 461 YIGKEALDI-PIDANYNIH 514
            +G+EAL + P+D  YNIH
Sbjct: 166 LVGEEALYVNPLDC-YNIH 183
>sp|Q8R2S9|ARP8_MOUSE Actin-related protein 8
          Length = 624

 Score = 60.5 bits (145), Expect = 6e-09
 Identities = 32/93 (34%), Positives = 53/93 (56%)
 Frame = +3

Query: 552 SKTAAVKDLEDIWRTGLEKLLKIPSKDLSNYRVILVIPDLVIRCEIRWITEMLIYQFGI* 731
           S TA + D+E IW   ++K L+IP KDL  YR IL+IPD+  +  ++ +  M++ + G  
Sbjct: 200 SLTAVLADIEVIWSHAIQKYLEIPLKDLKYYRCILLIPDIYNKQHVKELVHMILMKMGFA 259

Query: 732 *YRVTTRECLCYIQAPGLPTACVVNMHENSISI 830
              V  +E +C     GL + CVV++ +   S+
Sbjct: 260 GI-VVHQESVCATFGSGLSSTCVVDVGDQKTSV 291

 Score = 53.1 bits (126), Expect = 9e-07
 Identities = 48/145 (33%), Positives = 68/145 (46%), Gaps = 10/145 (6%)
 Frame = +2

Query: 110 IIVIHPSSTKLRFGRSNDSEPIIIPHCIARKSKKLSKVVRQPLIPDN------RTCTYIN 271
           I+VIHP ST LR GR+ D+ P+ +PH IAR+ K+      QPL  DN            N
Sbjct: 48  IVVIHPGSTTLRLGRATDTLPVSVPHVIARRHKQQG----QPLYKDNWLLREGLNKPESN 103

Query: 272 TKFNNAIKLASIAM---KSSSVEALQPYKEQPTINELIQMNKQYLKLESLLDMQDNDIQR 442
            +  N +K+   A+   K S+     P   + T +   QM    L   S     +   Q 
Sbjct: 104 EQRQNGLKMVDQAIWSKKMSNGTRRIPVSPEQTRSYNKQMRPAILDHCSGNKWTNTSQQ- 162

Query: 443 VYFEEFYIGKEALDI-PIDANYNIH 514
               E+ +G+EAL + P+D  YNIH
Sbjct: 163 ---PEYLVGEEALYVNPLDC-YNIH 183
>sp|Q9US07|ARP8_SCHPO Probable actin-related protein 8
          Length = 620

 Score = 52.4 bits (124), Expect = 2e-06
 Identities = 33/99 (33%), Positives = 49/99 (49%), Gaps = 2/99 (2%)
 Frame = +3

Query: 540 SDKYSKTAAVKDLEDIWRTGLEKLLKIPSKDLSNYRVILVIPDLVIRCEIRWITEMLIY- 716
           SD  S    + D+ +I++  +  LL+IP   LS Y VI ++PDL  R  +  I ++L + 
Sbjct: 210 SDYASSQQLLADIYEIFKYSITSLLQIPVSQLSQYSVIFIVPDLYDRVYVEKILDILFFD 269

Query: 717 -QFGI**YRVTTRECLCYIQAPGLPTACVVNMHENSISI 830
             FG        +E LC     G+  ACVV+M     SI
Sbjct: 270 LHFG---KAAIVQESLCTSFGAGMSAACVVDMGAQKTSI 305

 Score = 31.2 bits (69), Expect = 3.7
 Identities = 35/147 (23%), Positives = 67/147 (45%), Gaps = 19/147 (12%)
 Frame = +2

Query: 113 IVIHPSSTKLRFGRSNDSEPIIIPHCIARKSKKLSKVVRQPLIPDN---RTCTYIN---- 271
           IV+H  S  LR G +++  P  +P  IARK       +R P   +    +   ++N    
Sbjct: 56  IVLHIGSQNLRIGLASNKTPTTVPMVIARK-------MRAPFAQERCLLKDICHVNEDGN 108

Query: 272 ----TKFNNAIKLASIAMKSSSVEALQPYKEQPTINELIQMNKQYLKLESLLDMQDNDIQ 439
               ++F++ +KL    +KS      Q  +  P   +L++   +  K E++    D+D +
Sbjct: 109 VAFDSEFDSNLKLLDSELKSWL--KAQKKRSVPNGTQLVKNYNKISKPETV--PPDDDPE 164

Query: 440 R---VYFE-----EFYIGKEALDIPID 496
           +   ++FE     +   GKEA  +P++
Sbjct: 165 KPDWIHFEQDDHVDVICGKEAFLLPLN 191
>sp|Q9VX09|ARP8_DROME Probable actin-related protein 8
          Length = 607

 Score = 42.0 bits (97), Expect = 0.002
 Identities = 27/95 (28%), Positives = 48/95 (50%), Gaps = 2/95 (2%)
 Frame = +3

Query: 552 SKTAAVKDLEDIWRTGLEKLLKIPSKDLSNYRVILVIPDLVIRCEIRWITEMLIYQFGI* 731
           S  A+++ LE IW   LE+ LKIP + L  +  +LV+ D+ +R  +R    +L+ + G  
Sbjct: 195 SLQASMQHLERIWSYALEERLKIPLRKLGTHCAVLVVNDVYVRRHLREFVTLLLRRLG-- 252

Query: 732 *YR--VTTRECLCYIQAPGLPTACVVNMHENSISI 830
            +R     ++ +      G+   CVV++     SI
Sbjct: 253 -FRRCFLVQDSVASTFGAGIGYGCVVDIGAQKTSI 286

 Score = 36.6 bits (83), Expect = 0.089
 Identities = 19/47 (40%), Positives = 26/47 (55%), Gaps = 2/47 (4%)
 Frame = +2

Query: 110 IIVIHPSSTKLRFGRSNDSEPIIIPHCIA--RKSKKLSKVVRQPLIP 244
           IIVIHP S  LR GR+ D  P+ + H +A  R+     +    PL+P
Sbjct: 35  IIVIHPGSQHLRIGRAADLNPLTLLHAVAYRRRPGASDRPHHDPLLP 81
>sp|Q12386|ARP8_YEAST Actin-like protein ARP8
          Length = 881

 Score = 37.4 bits (85), Expect = 0.052
 Identities = 14/34 (41%), Positives = 21/34 (61%)
 Frame = +2

Query: 113 IVIHPSSTKLRFGRSNDSEPIIIPHCIARKSKKL 214
           IVIHP S  ++ G   D  P+++P+C+A   K L
Sbjct: 269 IVIHPGSNSIKIGFPKDDHPVVVPNCVAVPKKWL 302
>sp|P34529|DCR1_CAEEL Endoribonuclease dcr-1
          Length = 1845

 Score = 33.5 bits (75), Expect = 0.76
 Identities = 24/99 (24%), Positives = 50/99 (50%), Gaps = 7/99 (7%)
 Frame = +2

Query: 206 KKLSKVVRQPLIPDNRTCTYINTKFNNAIKLASIA----MKSSSVEALQPYKEQPTIN-- 367
           K+L K+++  ++PD +T  ++N    + I +  +      K  S+EAL+PY  Q  I   
Sbjct: 304 KELGKIIKSQVLPD-KTLRFLNMAKTSMITIKRLLEPEMKKIKSIEALRPYVPQRVIRLF 362

Query: 368 ELIQ-MNKQYLKLESLLDMQDNDIQRVYFEEFYIGKEAL 481
           E+++  N ++ K    L+  ++    ++ ++ YI    L
Sbjct: 363 EILETFNPEFQKERMKLEKAEHLSAIIFVDQRYIAYSLL 401
>sp|Q89B04|PT1_BUCBP Phosphoenolpyruvate-protein phosphotransferase (Phosphotransferase
           system, enzyme I)
          Length = 576

 Score = 33.1 bits (74), Expect = 0.99
 Identities = 19/49 (38%), Positives = 32/49 (65%), Gaps = 2/49 (4%)
 Frame = +3

Query: 531 DKISDKYSKTAAVKDLEDIWRTGLEKLLKIPSKDLSNYR--VILVIPDL 671
           ++++D+Y K  A+ D++DI    L+ +L I  KDL+N +  VIL+  DL
Sbjct: 116 EQLNDEYLKNRAI-DIQDIGNRLLKNILNIDIKDLNNIKNPVILIARDL 163
>sp|Q9PQH1|IF2_UREPA Translation initiation factor IF-2
          Length = 614

 Score = 32.3 bits (72), Expect = 1.7
 Identities = 31/120 (25%), Positives = 53/120 (44%), Gaps = 4/120 (3%)
 Frame = +2

Query: 107 NIIVIHPSSTKLRFGRSNDSEPIIIPHCIARKSKKLSKVVRQPLIPDNRTCTYINTKFN- 283
           N  +I+     L        +PIII   I     K+ K+ +   +     C  IN K   
Sbjct: 490 NYDIIYKFKEDLELWMKGTLDPIIIEEVIGEA--KVLKLFKHSQVGTICGCRVINGKIKR 547

Query: 284 NAIKLA---SIAMKSSSVEALQPYKEQPTINELIQMNKQYLKLESLLDMQDNDIQRVYFE 454
           NA+       I + +S +  LQ  K+  ++NE+I   +  L + +  D+++NDI  VY +
Sbjct: 548 NALVRVLRDGIVIYNSKIATLQHNKD--SVNEVIADKECGLTIANFNDIKENDIIEVYIK 605
>sp|P42928|SPI1_RABPU Serine proteinase inhibitor 1 (Serpin 1) (Serp-1)
          Length = 357

 Score = 31.2 bits (69), Expect = 3.7
 Identities = 13/54 (24%), Positives = 27/54 (50%)
 Frame = +2

Query: 341 PYKEQPTINELIQMNKQYLKLESLLDMQDNDIQRVYFEEFYIGKEALDIPIDAN 502
           P+ +  T  +   ++K  +    ++   +ND+Q V+  E + G   +DIP + N
Sbjct: 156 PFSKHLTYTDKFYISKNIVTSVDMMVSTENDLQYVHINELFGGFSIIDIPYEGN 209
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,692,381
Number of Sequences: 369166
Number of extensions: 1734470
Number of successful extensions: 3837
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3725
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3830
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8245425915
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)