Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_014_B09
(843 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P59679|ARP8_BRARE Actin-related protein 8 67 5e-11
sp|Q9H981|ARP8_HUMAN Actin-related protein 8 61 4e-09
sp|Q8R2S9|ARP8_MOUSE Actin-related protein 8 60 6e-09
sp|Q9US07|ARP8_SCHPO Probable actin-related protein 8 52 2e-06
sp|Q9VX09|ARP8_DROME Probable actin-related protein 8 42 0.002
sp|Q12386|ARP8_YEAST Actin-like protein ARP8 37 0.052
sp|P34529|DCR1_CAEEL Endoribonuclease dcr-1 33 0.76
sp|Q89B04|PT1_BUCBP Phosphoenolpyruvate-protein phosphotran... 33 0.99
sp|Q9PQH1|IF2_UREPA Translation initiation factor IF-2 32 1.7
sp|P42928|SPI1_RABPU Serine proteinase inhibitor 1 (Serpin ... 31 3.7
>sp|P59679|ARP8_BRARE Actin-related protein 8
Length = 623
Score = 67.4 bits (163), Expect = 5e-11
Identities = 38/93 (40%), Positives = 55/93 (59%)
Frame = +3
Query: 552 SKTAAVKDLEDIWRTGLEKLLKIPSKDLSNYRVILVIPDLVIRCEIRWITEMLIYQFGI* 731
S +A + DLE IW L+KLL+IP KDL YR IL+IPD+ R ++ I ML+ + G
Sbjct: 200 SLSAVMMDLEHIWTHALQKLLQIPLKDLKYYRCILLIPDIYNRQHVKEIVNMLLVKMGF- 258
Query: 732 *YRVTTRECLCYIQAPGLPTACVVNMHENSISI 830
V +E +C GL +ACVV++ + S+
Sbjct: 259 SAVVVHQESVCATFGSGLSSACVVDVGDQKTSV 291
Score = 47.4 bits (111), Expect = 5e-05
Identities = 36/139 (25%), Positives = 66/139 (47%), Gaps = 4/139 (2%)
Frame = +2
Query: 110 IIVIHPSSTKLRFGRSNDSEPIIIPHCIARKSKK--LSKVVRQPLIPDNRTCTYINTKFN 283
I+VIHP S LR GR+ D+ PI +PH IAR+ K S+ + L+ + N +
Sbjct: 48 IVVIHPGSRTLRLGRATDTLPISVPHVIARRHKHPGQSRYEDKCLLREGLNSADSNEQRQ 107
Query: 284 NAIKLASIAMKSSSVEALQPYKEQPTINELIQMNKQYLKLESLLDMQD--NDIQRVYFEE 457
N +K+ + S + + P E ++ + ++ ++LD + + E
Sbjct: 108 NGLKMVDQVIWSKKMS--NGVRRTPVSAEQARLYNRQIR-PAVLDPNSKVSWTNTSHHPE 164
Query: 458 FYIGKEALDIPIDANYNIH 514
+ +G+EAL + Y++H
Sbjct: 165 YVVGEEALYVNPTDCYSVH 183
>sp|Q9H981|ARP8_HUMAN Actin-related protein 8
Length = 624
Score = 60.8 bits (146), Expect = 4e-09
Identities = 31/93 (33%), Positives = 53/93 (56%)
Frame = +3
Query: 552 SKTAAVKDLEDIWRTGLEKLLKIPSKDLSNYRVILVIPDLVIRCEIRWITEMLIYQFGI* 731
S TA + D+E IW ++K L+IP KDL YR IL+IPD+ + ++ + M++ + G
Sbjct: 200 SLTAVLADIEVIWSHAIQKYLEIPLKDLKYYRCILLIPDIYNKQHVKELVNMILMKMGFS 259
Query: 732 *YRVTTRECLCYIQAPGLPTACVVNMHENSISI 830
V +E +C GL + C+V++ + S+
Sbjct: 260 GI-VVHQESVCATYGSGLSSTCIVDVGDQKTSV 291
Score = 51.6 bits (122), Expect = 3e-06
Identities = 42/139 (30%), Positives = 67/139 (48%), Gaps = 4/139 (2%)
Frame = +2
Query: 110 IIVIHPSSTKLRFGRSNDSEPIIIPHCIARKSKKLSKVVRQP--LIPDNRTCTYINTKFN 283
IIVIHP ST LR GR+ D+ P IPH IAR+ K+ + + + L+ + N +
Sbjct: 48 IIVIHPGSTTLRIGRATDTLPASIPHVIARRHKQQGQPLYKDSWLLREGLNKPESNEQRQ 107
Query: 284 NAIKLASIAMKSSSVEALQPYKEQPTINELIQMNKQYLKLESLLDMQDND-IQRVYFEEF 460
N +K+ A+ S + + P E + + ++ L N + E+
Sbjct: 108 NGLKMVDQAIWSKKMS--NGTRRIPVSPEQARSYNKQMRPAILDHCSGNKWTNTSHHPEY 165
Query: 461 YIGKEALDI-PIDANYNIH 514
+G+EAL + P+D YNIH
Sbjct: 166 LVGEEALYVNPLDC-YNIH 183
>sp|Q8R2S9|ARP8_MOUSE Actin-related protein 8
Length = 624
Score = 60.5 bits (145), Expect = 6e-09
Identities = 32/93 (34%), Positives = 53/93 (56%)
Frame = +3
Query: 552 SKTAAVKDLEDIWRTGLEKLLKIPSKDLSNYRVILVIPDLVIRCEIRWITEMLIYQFGI* 731
S TA + D+E IW ++K L+IP KDL YR IL+IPD+ + ++ + M++ + G
Sbjct: 200 SLTAVLADIEVIWSHAIQKYLEIPLKDLKYYRCILLIPDIYNKQHVKELVHMILMKMGFA 259
Query: 732 *YRVTTRECLCYIQAPGLPTACVVNMHENSISI 830
V +E +C GL + CVV++ + S+
Sbjct: 260 GI-VVHQESVCATFGSGLSSTCVVDVGDQKTSV 291
Score = 53.1 bits (126), Expect = 9e-07
Identities = 48/145 (33%), Positives = 68/145 (46%), Gaps = 10/145 (6%)
Frame = +2
Query: 110 IIVIHPSSTKLRFGRSNDSEPIIIPHCIARKSKKLSKVVRQPLIPDN------RTCTYIN 271
I+VIHP ST LR GR+ D+ P+ +PH IAR+ K+ QPL DN N
Sbjct: 48 IVVIHPGSTTLRLGRATDTLPVSVPHVIARRHKQQG----QPLYKDNWLLREGLNKPESN 103
Query: 272 TKFNNAIKLASIAM---KSSSVEALQPYKEQPTINELIQMNKQYLKLESLLDMQDNDIQR 442
+ N +K+ A+ K S+ P + T + QM L S + Q
Sbjct: 104 EQRQNGLKMVDQAIWSKKMSNGTRRIPVSPEQTRSYNKQMRPAILDHCSGNKWTNTSQQ- 162
Query: 443 VYFEEFYIGKEALDI-PIDANYNIH 514
E+ +G+EAL + P+D YNIH
Sbjct: 163 ---PEYLVGEEALYVNPLDC-YNIH 183
>sp|Q9US07|ARP8_SCHPO Probable actin-related protein 8
Length = 620
Score = 52.4 bits (124), Expect = 2e-06
Identities = 33/99 (33%), Positives = 49/99 (49%), Gaps = 2/99 (2%)
Frame = +3
Query: 540 SDKYSKTAAVKDLEDIWRTGLEKLLKIPSKDLSNYRVILVIPDLVIRCEIRWITEMLIY- 716
SD S + D+ +I++ + LL+IP LS Y VI ++PDL R + I ++L +
Sbjct: 210 SDYASSQQLLADIYEIFKYSITSLLQIPVSQLSQYSVIFIVPDLYDRVYVEKILDILFFD 269
Query: 717 -QFGI**YRVTTRECLCYIQAPGLPTACVVNMHENSISI 830
FG +E LC G+ ACVV+M SI
Sbjct: 270 LHFG---KAAIVQESLCTSFGAGMSAACVVDMGAQKTSI 305
Score = 31.2 bits (69), Expect = 3.7
Identities = 35/147 (23%), Positives = 67/147 (45%), Gaps = 19/147 (12%)
Frame = +2
Query: 113 IVIHPSSTKLRFGRSNDSEPIIIPHCIARKSKKLSKVVRQPLIPDN---RTCTYIN---- 271
IV+H S LR G +++ P +P IARK +R P + + ++N
Sbjct: 56 IVLHIGSQNLRIGLASNKTPTTVPMVIARK-------MRAPFAQERCLLKDICHVNEDGN 108
Query: 272 ----TKFNNAIKLASIAMKSSSVEALQPYKEQPTINELIQMNKQYLKLESLLDMQDNDIQ 439
++F++ +KL +KS Q + P +L++ + K E++ D+D +
Sbjct: 109 VAFDSEFDSNLKLLDSELKSWL--KAQKKRSVPNGTQLVKNYNKISKPETV--PPDDDPE 164
Query: 440 R---VYFE-----EFYIGKEALDIPID 496
+ ++FE + GKEA +P++
Sbjct: 165 KPDWIHFEQDDHVDVICGKEAFLLPLN 191
>sp|Q9VX09|ARP8_DROME Probable actin-related protein 8
Length = 607
Score = 42.0 bits (97), Expect = 0.002
Identities = 27/95 (28%), Positives = 48/95 (50%), Gaps = 2/95 (2%)
Frame = +3
Query: 552 SKTAAVKDLEDIWRTGLEKLLKIPSKDLSNYRVILVIPDLVIRCEIRWITEMLIYQFGI* 731
S A+++ LE IW LE+ LKIP + L + +LV+ D+ +R +R +L+ + G
Sbjct: 195 SLQASMQHLERIWSYALEERLKIPLRKLGTHCAVLVVNDVYVRRHLREFVTLLLRRLG-- 252
Query: 732 *YR--VTTRECLCYIQAPGLPTACVVNMHENSISI 830
+R ++ + G+ CVV++ SI
Sbjct: 253 -FRRCFLVQDSVASTFGAGIGYGCVVDIGAQKTSI 286
Score = 36.6 bits (83), Expect = 0.089
Identities = 19/47 (40%), Positives = 26/47 (55%), Gaps = 2/47 (4%)
Frame = +2
Query: 110 IIVIHPSSTKLRFGRSNDSEPIIIPHCIA--RKSKKLSKVVRQPLIP 244
IIVIHP S LR GR+ D P+ + H +A R+ + PL+P
Sbjct: 35 IIVIHPGSQHLRIGRAADLNPLTLLHAVAYRRRPGASDRPHHDPLLP 81
>sp|Q12386|ARP8_YEAST Actin-like protein ARP8
Length = 881
Score = 37.4 bits (85), Expect = 0.052
Identities = 14/34 (41%), Positives = 21/34 (61%)
Frame = +2
Query: 113 IVIHPSSTKLRFGRSNDSEPIIIPHCIARKSKKL 214
IVIHP S ++ G D P+++P+C+A K L
Sbjct: 269 IVIHPGSNSIKIGFPKDDHPVVVPNCVAVPKKWL 302
>sp|P34529|DCR1_CAEEL Endoribonuclease dcr-1
Length = 1845
Score = 33.5 bits (75), Expect = 0.76
Identities = 24/99 (24%), Positives = 50/99 (50%), Gaps = 7/99 (7%)
Frame = +2
Query: 206 KKLSKVVRQPLIPDNRTCTYINTKFNNAIKLASIA----MKSSSVEALQPYKEQPTIN-- 367
K+L K+++ ++PD +T ++N + I + + K S+EAL+PY Q I
Sbjct: 304 KELGKIIKSQVLPD-KTLRFLNMAKTSMITIKRLLEPEMKKIKSIEALRPYVPQRVIRLF 362
Query: 368 ELIQ-MNKQYLKLESLLDMQDNDIQRVYFEEFYIGKEAL 481
E+++ N ++ K L+ ++ ++ ++ YI L
Sbjct: 363 EILETFNPEFQKERMKLEKAEHLSAIIFVDQRYIAYSLL 401
>sp|Q89B04|PT1_BUCBP Phosphoenolpyruvate-protein phosphotransferase (Phosphotransferase
system, enzyme I)
Length = 576
Score = 33.1 bits (74), Expect = 0.99
Identities = 19/49 (38%), Positives = 32/49 (65%), Gaps = 2/49 (4%)
Frame = +3
Query: 531 DKISDKYSKTAAVKDLEDIWRTGLEKLLKIPSKDLSNYR--VILVIPDL 671
++++D+Y K A+ D++DI L+ +L I KDL+N + VIL+ DL
Sbjct: 116 EQLNDEYLKNRAI-DIQDIGNRLLKNILNIDIKDLNNIKNPVILIARDL 163
>sp|Q9PQH1|IF2_UREPA Translation initiation factor IF-2
Length = 614
Score = 32.3 bits (72), Expect = 1.7
Identities = 31/120 (25%), Positives = 53/120 (44%), Gaps = 4/120 (3%)
Frame = +2
Query: 107 NIIVIHPSSTKLRFGRSNDSEPIIIPHCIARKSKKLSKVVRQPLIPDNRTCTYINTKFN- 283
N +I+ L +PIII I K+ K+ + + C IN K
Sbjct: 490 NYDIIYKFKEDLELWMKGTLDPIIIEEVIGEA--KVLKLFKHSQVGTICGCRVINGKIKR 547
Query: 284 NAIKLA---SIAMKSSSVEALQPYKEQPTINELIQMNKQYLKLESLLDMQDNDIQRVYFE 454
NA+ I + +S + LQ K+ ++NE+I + L + + D+++NDI VY +
Sbjct: 548 NALVRVLRDGIVIYNSKIATLQHNKD--SVNEVIADKECGLTIANFNDIKENDIIEVYIK 605
>sp|P42928|SPI1_RABPU Serine proteinase inhibitor 1 (Serpin 1) (Serp-1)
Length = 357
Score = 31.2 bits (69), Expect = 3.7
Identities = 13/54 (24%), Positives = 27/54 (50%)
Frame = +2
Query: 341 PYKEQPTINELIQMNKQYLKLESLLDMQDNDIQRVYFEEFYIGKEALDIPIDAN 502
P+ + T + ++K + ++ +ND+Q V+ E + G +DIP + N
Sbjct: 156 PFSKHLTYTDKFYISKNIVTSVDMMVSTENDLQYVHINELFGGFSIIDIPYEGN 209
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,692,381
Number of Sequences: 369166
Number of extensions: 1734470
Number of successful extensions: 3837
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3725
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3830
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8245425915
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)