Planaria EST Database


DrC_01965

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01965
         (888 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q14674|ESPL1_HUMAN  Separin (Separase) (Caspase-like prot...    83   1e-15
sp|P60330|ESPL1_MOUSE  Separin (Separase) (Caspase-like prot...    82   3e-15
sp|P33144|BIMB_EMENI  Separin (Separase) (Cell division-asso...    69   1e-11
sp|Q03018|ESP1_YEAST  Separin (Separase)                           60   6e-09
sp|P18296|CUT1_SCHPO  Separin (Separase) (Cell untimely torn...    55   2e-07
sp|Q8D2G6|DXR_WIGBR  1-deoxy-D-xylulose 5-phosphate reductoi...    31   4.0  
sp|Q63WB7|LGT_BURPS  Prolipoprotein diacylglyceryl transfera...    31   5.3  
sp|P04028|YP1_AGRT4  Hypothetical protein 1 (Gene 5 protein)       30   6.9  
sp|P11046|LAMB1_DROME  Laminin beta-1 chain precursor (Lamin...    30   6.9  
sp|P11346|KRAF1_DROME  Raf homolog serine/threonine-protein ...    30   9.0  
>sp|Q14674|ESPL1_HUMAN Separin (Separase) (Caspase-like protein ESPL1) (Extra spindle
            poles-like 1 protein)
          Length = 2120

 Score = 82.8 bits (203), Expect = 1e-15
 Identities = 45/176 (25%), Positives = 85/176 (48%)
 Frame = +2

Query: 263  IIISDRNICRVPMESILIRDETNSKCITRMPNIVCLIGHLYQSRYRRVDAPQALVSRSNL 442
            +++ D+++ ++P ES+       +  +TR+P+   L+ +     Y        ++S+   
Sbjct: 1892 VLVLDKDLQKLPWESM---PSLQALPVTRLPSFRFLLSYSIIKEY----GASPVLSQG-- 1942

Query: 443  EIDPMKTFFLVNPDNSLQETEDTVLPLFKKQLCWEGVSGKSDKRIDPFNILSNKDMLIYC 622
             +DP  TF+++NP N+L  TE+     F  +  W GV G+  +       L+  D+ IY 
Sbjct: 1943 -VDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYA 2001

Query: 623  GHGAGGRYFMDRTYKSITSPTSLFLIGCQSSRFTVEGRCEAFGMIMYYLTIGLSIY 790
            GHGAG R+   +    ++      L GC S+   V G  E  G+++ Y+  G  ++
Sbjct: 2002 GHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLF 2057

 Score = 48.1 bits (113), Expect = 3e-05
 Identities = 19/30 (63%), Positives = 23/30 (76%)
 Frame = +3

Query: 777  GCPSILGLLWDVTDREIDKFTVKMLTNWLG 866
            GCP  LG LWDVTDR+ID++T  +L  WLG
Sbjct: 2053 GCPLFLGNLWDVTDRDIDRYTEALLQGWLG 2082
>sp|P60330|ESPL1_MOUSE Separin (Separase) (Caspase-like protein ESPL1) (Extra spindle
            poles-like 1 protein)
          Length = 2118

 Score = 81.6 bits (200), Expect = 3e-15
 Identities = 45/176 (25%), Positives = 85/176 (48%)
 Frame = +2

Query: 263  IIISDRNICRVPMESILIRDETNSKCITRMPNIVCLIGHLYQSRYRRVDAPQALVSRSNL 442
            +++ D+++ ++P ES  I     ++ +TR+P+   L+ +        V       S  + 
Sbjct: 1888 VLVLDKDLQKLPWESTPI---LQAQPVTRLPSFRFLLSYT-------VTKEAGASSVLSQ 1937

Query: 443  EIDPMKTFFLVNPDNSLQETEDTVLPLFKKQLCWEGVSGKSDKRIDPFNILSNKDMLIYC 622
             +DP  TF+++NP ++L  TE+     F  +  W+GV G+          L+ +D+ IY 
Sbjct: 1938 GVDPQNTFYVLNPHSNLSSTEERFRASFSSETGWKGVIGEVPSLDQVQAALTERDLYIYA 1997

Query: 623  GHGAGGRYFMDRTYKSITSPTSLFLIGCQSSRFTVEGRCEAFGMIMYYLTIGLSIY 790
            GHGAG R+   +    ++      L GC S+   V G  E  G+++ Y+  G  ++
Sbjct: 1998 GHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLF 2053

 Score = 48.1 bits (113), Expect = 3e-05
 Identities = 19/30 (63%), Positives = 23/30 (76%)
 Frame = +3

Query: 777  GCPSILGLLWDVTDREIDKFTVKMLTNWLG 866
            GCP  LG LWDVTDR+ID++T  +L  WLG
Sbjct: 2049 GCPLFLGNLWDVTDRDIDRYTEALLQGWLG 2078

 Score = 30.4 bits (67), Expect = 6.9
 Identities = 10/42 (23%), Positives = 21/42 (50%)
 Frame = +2

Query: 62   LLDQLENIKTQSTKGLKITERKAWWNNRFTVDSNLRSLINQM 187
            +L + + I+    +    TE++ WW  R  +D  + +LI  +
Sbjct: 1749 VLKEFDAIQKDQKENSSCTEKRVWWTGRLALDQRMEALITAL 1790
>sp|P33144|BIMB_EMENI Separin (Separase) (Cell division-associated protein bimB)
          Length = 2067

 Score = 69.3 bits (168), Expect = 1e-11
 Identities = 47/176 (26%), Positives = 80/176 (45%), Gaps = 4/176 (2%)
 Frame = +2

Query: 263  IIISDRNICRVPMESILIRDETNSKCITRMPNIVCLIGHLYQSRYRRVDAPQALVSRSNL 442
            +++ D+++   P ES+          + R+P++ CL   +   R  +         +S L
Sbjct: 1829 VLVLDKSLHLFPWESLPC---LQGLPVCRVPSLECLRDRVLHLRSGK---------QSAL 1876

Query: 443  EIDPMKTFFLVNPDNSLQETEDTVLPLFKKQLC----WEGVSGKSDKRIDPFNILSNKDM 610
             ID     +++NP   L+ T++T    F+K L     W G+  +     +  + L +K +
Sbjct: 1877 SIDRRNGTYILNPTGDLKTTQET----FEKDLSSLKGWTGMVNRQPTEDEFKDSLQSKSL 1932

Query: 611  LIYCGHGAGGRYFMDRTYKSITSPTSLFLIGCQSSRFTVEGRCEAFGMIMYYLTIG 778
             +Y GHG+G +Y   RT K +      FL+GC S   T  G  E +G  M YL  G
Sbjct: 1933 FLYFGHGSGAQYIRGRTVKRLDRCAVAFLMGCSSGTLTEAGEYEPYGTPMNYLQAG 1988

 Score = 37.4 bits (85), Expect = 0.056
 Identities = 15/37 (40%), Positives = 22/37 (59%)
 Frame = +3

Query: 771  QLGCPSILGLLWDVTDREIDKFTVKMLTNWLGKSNHH 881
            Q G P+++  LWDVTD++ID+F      +W    N H
Sbjct: 1986 QAGSPALVATLWDVTDKDIDRFAKATFEHWGLIGNGH 2022

 Score = 32.7 bits (73), Expect = 1.4
 Identities = 17/54 (31%), Positives = 28/54 (51%), Gaps = 2/54 (3%)
 Frame = +2

Query: 47   RTGMQLLDQLENIKTQSTKGLKITE--RKAWWNNRFTVDSNLRSLINQMRNDWF 202
            R  M+ L +L N    + K L++    +K WW NR  +D  + +L+  + N WF
Sbjct: 1650 RDEMKELIRLANESAHAAK-LQVDRQMKKEWWKNREALDRRMENLLQNIENVWF 1702
>sp|Q03018|ESP1_YEAST Separin (Separase)
          Length = 1630

 Score = 60.5 bits (145), Expect = 6e-09
 Identities = 42/161 (26%), Positives = 71/161 (44%), Gaps = 4/161 (2%)
 Frame = +2

Query: 341  ITRMPNIVCLIGHLYQSRYRRVDAPQALVSRSNLEIDPMKTFFLVNPDNSLQETEDTVLP 520
            ITR+P+ VCL   L +  Y+    P  +    N+ +       ++NP+  L  TE     
Sbjct: 1416 ITRVPSYVCLNKLLSRFHYQ---LPLQVTIEDNISM-------ILNPNGDLSRTESKFKG 1465

Query: 521  LFKKQL----CWEGVSGKSDKRIDPFNILSNKDMLIYCGHGAGGRYFMDRTYKSITSPTS 688
            +F+K +      + V  +  +      +L N ++ +Y GHG G +Y   +  K  T    
Sbjct: 1466 MFQKIIDAKPSSQLVMNEKPEEETLLKMLQNSNLFVYIGHGGGEQYVRSKEIKKCTKIAP 1525

Query: 689  LFLIGCQSSRFTVEGRCEAFGMIMYYLTIGLSIYTWIIMGC 811
             FL+GC S+     G+ E  G          +IYT+++ GC
Sbjct: 1526 SFLLGCSSAAMKYYGKLEPTG----------TIYTYLLGGC 1556

 Score = 40.8 bits (94), Expect = 0.005
 Identities = 15/24 (62%), Positives = 21/24 (87%)
 Frame = +3

Query: 777  GCPSILGLLWDVTDREIDKFTVKM 848
            GCP +LG LWDVTD++IDKF+ ++
Sbjct: 1555 GCPMVLGNLWDVTDKDIDKFSEEL 1578

 Score = 39.7 bits (91), Expect = 0.011
 Identities = 12/28 (42%), Positives = 19/28 (67%)
 Frame = +2

Query: 119  ERKAWWNNRFTVDSNLRSLINQMRNDWF 202
            ERK+WW  R+ +D  ++ L+N + N WF
Sbjct: 1245 ERKSWWTTRYDLDKRMQQLLNNIENSWF 1272
>sp|P18296|CUT1_SCHPO Separin (Separase) (Cell untimely torn protein 1)
          Length = 1828

 Score = 55.5 bits (132), Expect = 2e-07
 Identities = 41/175 (23%), Positives = 79/175 (45%), Gaps = 3/175 (1%)
 Frame = +2

Query: 263  IIISDRNICRVPMESILIRDETNSKCITRMPNIVCLIGHLYQSRYRRVDAPQALVSRSNL 442
            +++ D+++ + P ES+      N + ++R+P++  L   L QS          +V+   +
Sbjct: 1596 VLVLDKSVHQFPWESLPC---LNRQSVSRVPSLSILRDILSQS---------FVVNGEYV 1643

Query: 443  EIDPMKTFFLVNPDNSLQETEDTVLPLFKKQLC---WEGVSGKSDKRIDPFNILSNKDML 613
            E+      +++NP   L+ T++    +F+ +L    W+G+        D   +LS  D  
Sbjct: 1644 EVRKEAGSYILNPSLDLKHTQE----MFEHKLVEGGWKGLIASQPSNRDFIKMLSGNDFF 1699

Query: 614  IYCGHGAGGRYFMDRTYKSITSPTSLFLIGCQSSRFTVEGRCEAFGMIMYYLTIG 778
            +Y GHG G +Y       ++       L+GC S      G  E +G  + YL+ G
Sbjct: 1700 LYFGHGGGEQYTTSYDLATLKRCAVTILMGCSSGALYECGSFEPWGTPLDYLSAG 1754

 Score = 46.2 bits (108), Expect = 1e-04
 Identities = 16/28 (57%), Positives = 25/28 (89%)
 Frame = +3

Query: 777  GCPSILGLLWDVTDREIDKFTVKMLTNW 860
            GCP+++  LWDVTD++ID+F++KML +W
Sbjct: 1754 GCPTLVANLWDVTDKDIDRFSLKMLESW 1781
>sp|Q8D2G6|DXR_WIGBR 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXP
           reductoisomerase) (1-deoxyxylulose-5-phosphate
           reductoisomerase) (2-C-methyl-D-erythritol 4-phosphate
           synthase)
          Length = 397

 Score = 31.2 bits (69), Expect = 4.0
 Identities = 34/127 (26%), Positives = 56/127 (44%), Gaps = 10/127 (7%)
 Frame = +2

Query: 332 SKCITRMPNIVCLIGHLYQSRYRRVDAPQALVSRSNLEIDPMKTFFLVNPDNSLQETEDT 511
           S+CI   PN VC++     ++ + +D  Q L+          KT  L   ++    +   
Sbjct: 45  SQCIKYKPNYVCIM-----NKKKLLDLKQGLLKNK------CKTSVLFGSNDICNLSSSK 93

Query: 512 VLPLFKKQLCWEGVSG--------KSDKRIDPFNILSNKDMLIYCGHGAGGRYFMDRT-- 661
            + +        G+SG        KS K+I    +L+NK++L+ CGH     +FM +   
Sbjct: 94  EVDIVISATV--GLSGIFFLFSAIKSGKKI----LLANKEILVSCGH-----FFMKQVEK 142

Query: 662 YKSITSP 682
           YKSI  P
Sbjct: 143 YKSIIIP 149
>sp|Q63WB7|LGT_BURPS Prolipoprotein diacylglyceryl transferase
 sp|Q62LG5|LGT_BURMA Prolipoprotein diacylglyceryl transferase
          Length = 296

 Score = 30.8 bits (68), Expect = 5.3
 Identities = 19/52 (36%), Positives = 30/52 (57%)
 Frame = +2

Query: 644 YFMDRTYKSITSPTSLFLIGCQSSRFTVEGRCEAFGMIMYYLTIGLSIYTWI 799
           +F  R  + + + ++LFLIG  ++RFTVE   E     +  LT GLS+  W+
Sbjct: 221 WFFSRKPRPMGAISALFLIGYGAARFTVEFAREP-DDFLGLLTFGLSMGQWL 271
>sp|P04028|YP1_AGRT4 Hypothetical protein 1 (Gene 5 protein)
          Length = 172

 Score = 30.4 bits (67), Expect = 6.9
 Identities = 18/70 (25%), Positives = 30/70 (42%)
 Frame = +2

Query: 467 FLVNPDNSLQETEDTVLPLFKKQLCWEGVSGKSDKRIDPFNILSNKDMLIYCGHGAGGRY 646
           F++N D  +   +D V+   +K  C        D    PF+IL+   + +YC      RY
Sbjct: 51  FIINTDVPIDPAKDEVVKCSRKVACCP------DPTDIPFDILNVSLLYVYCSFQEMRRY 104

Query: 647 FMDRTYKSIT 676
              R Y  ++
Sbjct: 105 AQQRFYDGVS 114
>sp|P11046|LAMB1_DROME Laminin beta-1 chain precursor (Laminin B1 chain)
          Length = 1790

 Score = 30.4 bits (67), Expect = 6.9
 Identities = 16/47 (34%), Positives = 23/47 (48%)
 Frame = +2

Query: 155  DSNLRSLINQMRNDWFTDSNLKWLMDEGNELEGEPKIIISDRNICRV 295
            +SN+   +N  R+ +   SNL  L DE NEL      I  +  IC +
Sbjct: 1324 ESNIEGALNLTRHAYERVSNLSTLKDEANELASNSDRIAREWKICLI 1370
>sp|P11346|KRAF1_DROME Raf homolog serine/threonine-protein kinase dRAF-1 (Pole-hole
           protein)
          Length = 739

 Score = 30.0 bits (66), Expect = 9.0
 Identities = 28/107 (26%), Positives = 49/107 (45%), Gaps = 11/107 (10%)
 Frame = -3

Query: 658 SIHEIATTCSVSTVYEHILVAENVERINTLIGLSRHTFPT*LFLKQRKNGIFCFLK-GIV 482
           S+  +   C  S++Y+H+ V+E   ++NTLI + R       +L   KN I   LK   +
Sbjct: 496 SLAIVTQWCEGSSLYKHVHVSETKFKLNTLIDIGRQVAQGMDYL-HAKNIIHRDLKSNNI 554

Query: 481 RIYQEKGFHGIDFKI----------RSRNQGLGSIYASVPALIQMSD 371
            ++++      DF +          +  NQ  GSI    P +I+M +
Sbjct: 555 FLHEDLSVKIGDFGLATAKTRWSGEKQANQPTGSILWMAPEVIRMQE 601
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 111,780,744
Number of Sequences: 369166
Number of extensions: 2424112
Number of successful extensions: 5766
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5545
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5764
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8886314050
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)