Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_012_N03
(888 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q14674|ESPL1_HUMAN Separin (Separase) (Caspase-like prot... 83 1e-15
sp|P60330|ESPL1_MOUSE Separin (Separase) (Caspase-like prot... 82 3e-15
sp|P33144|BIMB_EMENI Separin (Separase) (Cell division-asso... 69 1e-11
sp|Q03018|ESP1_YEAST Separin (Separase) 60 6e-09
sp|P18296|CUT1_SCHPO Separin (Separase) (Cell untimely torn... 55 2e-07
sp|Q8D2G6|DXR_WIGBR 1-deoxy-D-xylulose 5-phosphate reductoi... 31 4.0
sp|Q63WB7|LGT_BURPS Prolipoprotein diacylglyceryl transfera... 31 5.3
sp|P04028|YP1_AGRT4 Hypothetical protein 1 (Gene 5 protein) 30 6.9
sp|P11046|LAMB1_DROME Laminin beta-1 chain precursor (Lamin... 30 6.9
sp|P11346|KRAF1_DROME Raf homolog serine/threonine-protein ... 30 9.0
>sp|Q14674|ESPL1_HUMAN Separin (Separase) (Caspase-like protein ESPL1) (Extra spindle
poles-like 1 protein)
Length = 2120
Score = 82.8 bits (203), Expect = 1e-15
Identities = 45/176 (25%), Positives = 85/176 (48%)
Frame = +2
Query: 263 IIISDRNICRVPMESILIRDETNSKCITRMPNIVCLIGHLYQSRYRRVDAPQALVSRSNL 442
+++ D+++ ++P ES+ + +TR+P+ L+ + Y ++S+
Sbjct: 1892 VLVLDKDLQKLPWESM---PSLQALPVTRLPSFRFLLSYSIIKEY----GASPVLSQG-- 1942
Query: 443 EIDPMKTFFLVNPDNSLQETEDTVLPLFKKQLCWEGVSGKSDKRIDPFNILSNKDMLIYC 622
+DP TF+++NP N+L TE+ F + W GV G+ + L+ D+ IY
Sbjct: 1943 -VDPRSTFYVLNPHNNLSSTEEQFRANFSSEAGWRGVVGEVPRPEQVQEALTKHDLYIYA 2001
Query: 623 GHGAGGRYFMDRTYKSITSPTSLFLIGCQSSRFTVEGRCEAFGMIMYYLTIGLSIY 790
GHGAG R+ + ++ L GC S+ V G E G+++ Y+ G ++
Sbjct: 2002 GHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLF 2057
Score = 48.1 bits (113), Expect = 3e-05
Identities = 19/30 (63%), Positives = 23/30 (76%)
Frame = +3
Query: 777 GCPSILGLLWDVTDREIDKFTVKMLTNWLG 866
GCP LG LWDVTDR+ID++T +L WLG
Sbjct: 2053 GCPLFLGNLWDVTDRDIDRYTEALLQGWLG 2082
>sp|P60330|ESPL1_MOUSE Separin (Separase) (Caspase-like protein ESPL1) (Extra spindle
poles-like 1 protein)
Length = 2118
Score = 81.6 bits (200), Expect = 3e-15
Identities = 45/176 (25%), Positives = 85/176 (48%)
Frame = +2
Query: 263 IIISDRNICRVPMESILIRDETNSKCITRMPNIVCLIGHLYQSRYRRVDAPQALVSRSNL 442
+++ D+++ ++P ES I ++ +TR+P+ L+ + V S +
Sbjct: 1888 VLVLDKDLQKLPWESTPI---LQAQPVTRLPSFRFLLSYT-------VTKEAGASSVLSQ 1937
Query: 443 EIDPMKTFFLVNPDNSLQETEDTVLPLFKKQLCWEGVSGKSDKRIDPFNILSNKDMLIYC 622
+DP TF+++NP ++L TE+ F + W+GV G+ L+ +D+ IY
Sbjct: 1938 GVDPQNTFYVLNPHSNLSSTEERFRASFSSETGWKGVIGEVPSLDQVQAALTERDLYIYA 1997
Query: 623 GHGAGGRYFMDRTYKSITSPTSLFLIGCQSSRFTVEGRCEAFGMIMYYLTIGLSIY 790
GHGAG R+ + ++ L GC S+ V G E G+++ Y+ G ++
Sbjct: 1998 GHGAGARFLDGQAVLRLSCRAVALLFGCSSAALAVHGNLEGAGIVLKYIMAGCPLF 2053
Score = 48.1 bits (113), Expect = 3e-05
Identities = 19/30 (63%), Positives = 23/30 (76%)
Frame = +3
Query: 777 GCPSILGLLWDVTDREIDKFTVKMLTNWLG 866
GCP LG LWDVTDR+ID++T +L WLG
Sbjct: 2049 GCPLFLGNLWDVTDRDIDRYTEALLQGWLG 2078
Score = 30.4 bits (67), Expect = 6.9
Identities = 10/42 (23%), Positives = 21/42 (50%)
Frame = +2
Query: 62 LLDQLENIKTQSTKGLKITERKAWWNNRFTVDSNLRSLINQM 187
+L + + I+ + TE++ WW R +D + +LI +
Sbjct: 1749 VLKEFDAIQKDQKENSSCTEKRVWWTGRLALDQRMEALITAL 1790
>sp|P33144|BIMB_EMENI Separin (Separase) (Cell division-associated protein bimB)
Length = 2067
Score = 69.3 bits (168), Expect = 1e-11
Identities = 47/176 (26%), Positives = 80/176 (45%), Gaps = 4/176 (2%)
Frame = +2
Query: 263 IIISDRNICRVPMESILIRDETNSKCITRMPNIVCLIGHLYQSRYRRVDAPQALVSRSNL 442
+++ D+++ P ES+ + R+P++ CL + R + +S L
Sbjct: 1829 VLVLDKSLHLFPWESLPC---LQGLPVCRVPSLECLRDRVLHLRSGK---------QSAL 1876
Query: 443 EIDPMKTFFLVNPDNSLQETEDTVLPLFKKQLC----WEGVSGKSDKRIDPFNILSNKDM 610
ID +++NP L+ T++T F+K L W G+ + + + L +K +
Sbjct: 1877 SIDRRNGTYILNPTGDLKTTQET----FEKDLSSLKGWTGMVNRQPTEDEFKDSLQSKSL 1932
Query: 611 LIYCGHGAGGRYFMDRTYKSITSPTSLFLIGCQSSRFTVEGRCEAFGMIMYYLTIG 778
+Y GHG+G +Y RT K + FL+GC S T G E +G M YL G
Sbjct: 1933 FLYFGHGSGAQYIRGRTVKRLDRCAVAFLMGCSSGTLTEAGEYEPYGTPMNYLQAG 1988
Score = 37.4 bits (85), Expect = 0.056
Identities = 15/37 (40%), Positives = 22/37 (59%)
Frame = +3
Query: 771 QLGCPSILGLLWDVTDREIDKFTVKMLTNWLGKSNHH 881
Q G P+++ LWDVTD++ID+F +W N H
Sbjct: 1986 QAGSPALVATLWDVTDKDIDRFAKATFEHWGLIGNGH 2022
Score = 32.7 bits (73), Expect = 1.4
Identities = 17/54 (31%), Positives = 28/54 (51%), Gaps = 2/54 (3%)
Frame = +2
Query: 47 RTGMQLLDQLENIKTQSTKGLKITE--RKAWWNNRFTVDSNLRSLINQMRNDWF 202
R M+ L +L N + K L++ +K WW NR +D + +L+ + N WF
Sbjct: 1650 RDEMKELIRLANESAHAAK-LQVDRQMKKEWWKNREALDRRMENLLQNIENVWF 1702
>sp|Q03018|ESP1_YEAST Separin (Separase)
Length = 1630
Score = 60.5 bits (145), Expect = 6e-09
Identities = 42/161 (26%), Positives = 71/161 (44%), Gaps = 4/161 (2%)
Frame = +2
Query: 341 ITRMPNIVCLIGHLYQSRYRRVDAPQALVSRSNLEIDPMKTFFLVNPDNSLQETEDTVLP 520
ITR+P+ VCL L + Y+ P + N+ + ++NP+ L TE
Sbjct: 1416 ITRVPSYVCLNKLLSRFHYQ---LPLQVTIEDNISM-------ILNPNGDLSRTESKFKG 1465
Query: 521 LFKKQL----CWEGVSGKSDKRIDPFNILSNKDMLIYCGHGAGGRYFMDRTYKSITSPTS 688
+F+K + + V + + +L N ++ +Y GHG G +Y + K T
Sbjct: 1466 MFQKIIDAKPSSQLVMNEKPEEETLLKMLQNSNLFVYIGHGGGEQYVRSKEIKKCTKIAP 1525
Query: 689 LFLIGCQSSRFTVEGRCEAFGMIMYYLTIGLSIYTWIIMGC 811
FL+GC S+ G+ E G +IYT+++ GC
Sbjct: 1526 SFLLGCSSAAMKYYGKLEPTG----------TIYTYLLGGC 1556
Score = 40.8 bits (94), Expect = 0.005
Identities = 15/24 (62%), Positives = 21/24 (87%)
Frame = +3
Query: 777 GCPSILGLLWDVTDREIDKFTVKM 848
GCP +LG LWDVTD++IDKF+ ++
Sbjct: 1555 GCPMVLGNLWDVTDKDIDKFSEEL 1578
Score = 39.7 bits (91), Expect = 0.011
Identities = 12/28 (42%), Positives = 19/28 (67%)
Frame = +2
Query: 119 ERKAWWNNRFTVDSNLRSLINQMRNDWF 202
ERK+WW R+ +D ++ L+N + N WF
Sbjct: 1245 ERKSWWTTRYDLDKRMQQLLNNIENSWF 1272
>sp|P18296|CUT1_SCHPO Separin (Separase) (Cell untimely torn protein 1)
Length = 1828
Score = 55.5 bits (132), Expect = 2e-07
Identities = 41/175 (23%), Positives = 79/175 (45%), Gaps = 3/175 (1%)
Frame = +2
Query: 263 IIISDRNICRVPMESILIRDETNSKCITRMPNIVCLIGHLYQSRYRRVDAPQALVSRSNL 442
+++ D+++ + P ES+ N + ++R+P++ L L QS +V+ +
Sbjct: 1596 VLVLDKSVHQFPWESLPC---LNRQSVSRVPSLSILRDILSQS---------FVVNGEYV 1643
Query: 443 EIDPMKTFFLVNPDNSLQETEDTVLPLFKKQLC---WEGVSGKSDKRIDPFNILSNKDML 613
E+ +++NP L+ T++ +F+ +L W+G+ D +LS D
Sbjct: 1644 EVRKEAGSYILNPSLDLKHTQE----MFEHKLVEGGWKGLIASQPSNRDFIKMLSGNDFF 1699
Query: 614 IYCGHGAGGRYFMDRTYKSITSPTSLFLIGCQSSRFTVEGRCEAFGMIMYYLTIG 778
+Y GHG G +Y ++ L+GC S G E +G + YL+ G
Sbjct: 1700 LYFGHGGGEQYTTSYDLATLKRCAVTILMGCSSGALYECGSFEPWGTPLDYLSAG 1754
Score = 46.2 bits (108), Expect = 1e-04
Identities = 16/28 (57%), Positives = 25/28 (89%)
Frame = +3
Query: 777 GCPSILGLLWDVTDREIDKFTVKMLTNW 860
GCP+++ LWDVTD++ID+F++KML +W
Sbjct: 1754 GCPTLVANLWDVTDKDIDRFSLKMLESW 1781
>sp|Q8D2G6|DXR_WIGBR 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXP
reductoisomerase) (1-deoxyxylulose-5-phosphate
reductoisomerase) (2-C-methyl-D-erythritol 4-phosphate
synthase)
Length = 397
Score = 31.2 bits (69), Expect = 4.0
Identities = 34/127 (26%), Positives = 56/127 (44%), Gaps = 10/127 (7%)
Frame = +2
Query: 332 SKCITRMPNIVCLIGHLYQSRYRRVDAPQALVSRSNLEIDPMKTFFLVNPDNSLQETEDT 511
S+CI PN VC++ ++ + +D Q L+ KT L ++ +
Sbjct: 45 SQCIKYKPNYVCIM-----NKKKLLDLKQGLLKNK------CKTSVLFGSNDICNLSSSK 93
Query: 512 VLPLFKKQLCWEGVSG--------KSDKRIDPFNILSNKDMLIYCGHGAGGRYFMDRT-- 661
+ + G+SG KS K+I +L+NK++L+ CGH +FM +
Sbjct: 94 EVDIVISATV--GLSGIFFLFSAIKSGKKI----LLANKEILVSCGH-----FFMKQVEK 142
Query: 662 YKSITSP 682
YKSI P
Sbjct: 143 YKSIIIP 149
>sp|Q63WB7|LGT_BURPS Prolipoprotein diacylglyceryl transferase
sp|Q62LG5|LGT_BURMA Prolipoprotein diacylglyceryl transferase
Length = 296
Score = 30.8 bits (68), Expect = 5.3
Identities = 19/52 (36%), Positives = 30/52 (57%)
Frame = +2
Query: 644 YFMDRTYKSITSPTSLFLIGCQSSRFTVEGRCEAFGMIMYYLTIGLSIYTWI 799
+F R + + + ++LFLIG ++RFTVE E + LT GLS+ W+
Sbjct: 221 WFFSRKPRPMGAISALFLIGYGAARFTVEFAREP-DDFLGLLTFGLSMGQWL 271
>sp|P04028|YP1_AGRT4 Hypothetical protein 1 (Gene 5 protein)
Length = 172
Score = 30.4 bits (67), Expect = 6.9
Identities = 18/70 (25%), Positives = 30/70 (42%)
Frame = +2
Query: 467 FLVNPDNSLQETEDTVLPLFKKQLCWEGVSGKSDKRIDPFNILSNKDMLIYCGHGAGGRY 646
F++N D + +D V+ +K C D PF+IL+ + +YC RY
Sbjct: 51 FIINTDVPIDPAKDEVVKCSRKVACCP------DPTDIPFDILNVSLLYVYCSFQEMRRY 104
Query: 647 FMDRTYKSIT 676
R Y ++
Sbjct: 105 AQQRFYDGVS 114
>sp|P11046|LAMB1_DROME Laminin beta-1 chain precursor (Laminin B1 chain)
Length = 1790
Score = 30.4 bits (67), Expect = 6.9
Identities = 16/47 (34%), Positives = 23/47 (48%)
Frame = +2
Query: 155 DSNLRSLINQMRNDWFTDSNLKWLMDEGNELEGEPKIIISDRNICRV 295
+SN+ +N R+ + SNL L DE NEL I + IC +
Sbjct: 1324 ESNIEGALNLTRHAYERVSNLSTLKDEANELASNSDRIAREWKICLI 1370
>sp|P11346|KRAF1_DROME Raf homolog serine/threonine-protein kinase dRAF-1 (Pole-hole
protein)
Length = 739
Score = 30.0 bits (66), Expect = 9.0
Identities = 28/107 (26%), Positives = 49/107 (45%), Gaps = 11/107 (10%)
Frame = -3
Query: 658 SIHEIATTCSVSTVYEHILVAENVERINTLIGLSRHTFPT*LFLKQRKNGIFCFLK-GIV 482
S+ + C S++Y+H+ V+E ++NTLI + R +L KN I LK +
Sbjct: 496 SLAIVTQWCEGSSLYKHVHVSETKFKLNTLIDIGRQVAQGMDYL-HAKNIIHRDLKSNNI 554
Query: 481 RIYQEKGFHGIDFKI----------RSRNQGLGSIYASVPALIQMSD 371
++++ DF + + NQ GSI P +I+M +
Sbjct: 555 FLHEDLSVKIGDFGLATAKTRWSGEKQANQPTGSILWMAPEVIRMQE 601
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 111,780,744
Number of Sequences: 369166
Number of extensions: 2424112
Number of successful extensions: 5766
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5545
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5764
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8886314050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)