Planaria EST Database


DrC_01854

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01854
         (907 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O15886|PHLC_TRYCR  Variant-surface-glycoprotein phospholi...    80   1e-14
sp|P09194|PHLC_TRYBB  Variant-surface-glycoprotein phospholi...    74   7e-13
sp|P34024|PLC_LISMO  1-phosphatidylinositol phosphodiesteras...    41   0.004
sp|P45723|PLC_STAAU  1-phosphatidylinositol phosphodiesteras...    39   0.020
sp|P57225|SYT_BUCAI  Threonyl-tRNA synthetase (Threonine--tR...    33   0.84 
>sp|O15886|PHLC_TRYCR Variant-surface-glycoprotein phospholipase C (VSG lipase)
           (Glycosylphosphatidylinositol-specific phospholipase C)
           (GPI-PLC)
          Length = 380

 Score = 79.7 bits (195), Expect = 1e-14
 Identities = 68/244 (27%), Positives = 108/244 (44%), Gaps = 30/244 (12%)
 Frame = +2

Query: 164 SLFGSLAKSIIARWAKAQALNTFGQLDSGIRYFDIRVAKNSKDKDEIYSCHSLFGYPIKT 343
           SL   L + I A W++ Q ++   QL+ G+RY D+RVA N +D   +Y+ H     P+  
Sbjct: 69  SLLRFLFRGICASWSRCQWMSVRAQLNHGVRYLDMRVATNPEDASRLYTLHHQISVPLAD 128

Query: 344 DLLNIKDFLD--LHQKEVVLLDINHFY----NFGMNEHYRLIRHLETVFGNKLMSFSYQI 505
            L ++K FL+  L   E ++LD  H Y    + G  + +R +  L   F          +
Sbjct: 129 VLEDVKAFLNDPLSADEFIVLDFQHLYLTDDSDGKGKFFRELDRLSDRF------IPVDV 182

Query: 506 P---SLDEIWKTKS--RVIVFYHSNDVQRPYLWPGYFIPSP-----WANTDELTKLLDFL 655
           P    L+ +W+  S  R+ +   S + + PY  P   I S      W N + L KLL+ L
Sbjct: 183 PLTTPLEFLWRASSRRRIFLVVGSGEDESPY--PAARIRSKCMVSRWVNENSLRKLLEAL 240

Query: 656 ------TANYKRGRAIDYFHVSQGVLTPTTGDIV--------KHVFSSLKSVMADKATPA 793
                    Y +       +V+Q V TP   DI         + V SS+  V A +  P+
Sbjct: 241 DNLLLDDLKYPQTGVPSKLYVTQAVFTPRYSDIFLGIFPKISRRVVSSIYDV-ATRVNPS 299

Query: 794 FIKW 805
            ++W
Sbjct: 300 LLEW 303
>sp|P09194|PHLC_TRYBB Variant-surface-glycoprotein phospholipase C (VSG lipase)
           (Glycosylphosphatidylinositol-specific phospholipase C)
           (GPI-PLC)
          Length = 358

 Score = 73.6 bits (179), Expect = 7e-13
 Identities = 69/264 (26%), Positives = 111/264 (42%), Gaps = 26/264 (9%)
 Frame = +2

Query: 20  WMSNLPETLTCLPLNYIAIPGXXXXXXXXXXXXXXXAPDAEEIVKKL----TSLFGSLAK 187
           WMS+   ++    +  + + G                 DA E ++ L    T L   L  
Sbjct: 12  WMSDTRSSIEKKCIGQVYMVGAHNAGTHGIQMFSPFGLDAPEKLRSLPPYVTFLLRFLTV 71

Query: 188 SIIARWAKAQALNTFGQLDSGIRYFDIRVAKNSKDKDEIYSCHSLFGYPIKTDLLNIKDF 367
            + +RW + Q L+    LD G+RY D+R+  +   +++IY+ H     P++  L ++KDF
Sbjct: 72  GVSSRWGRCQNLSIRQLLDHGVRYLDLRMNISPDQENKIYTTHFHISVPLQEVLKDVKDF 131

Query: 368 L--DLHQKEVVLLDINHFYNFGMNEHYRLIRHLETVFGNKLMSFSYQIPS-------LDE 520
           L       E V+LD  HFY  G NE + + R +E +   +     + IP+       L  
Sbjct: 132 LTTPASANEFVILDFLHFY--GFNESHTMKRFVEELQALE----EFYIPTTVSLTTPLCN 185

Query: 521 IWKTKSRVIVFYHSNDVQRPYLWPGY------FIPSPWANTDELTKLLDFLTANYKR--- 673
           +W++  R+ +      V RPY+   Y       + S W N  EL  LLD L     R   
Sbjct: 186 LWQSTRRIFL------VVRPYVEYPYARLRSVALKSIWVNQMELNDLLDRLEELMTRDLE 239

Query: 674 ----GRAIDYFHVSQGVLTPTTGD 733
               G      +V+Q + TP   D
Sbjct: 240 DVSIGGVPSKMYVTQAIGTPRNND 263
>sp|P34024|PLC_LISMO 1-phosphatidylinositol phosphodiesterase precursor
           (Phosphatidylinositol diacylglycerol-lyase)
           (Phosphatidylinositol-specific phospholipase C) (PI-PLC)
          Length = 317

 Score = 41.2 bits (95), Expect = 0.004
 Identities = 36/144 (25%), Positives = 58/144 (40%)
 Frame = +2

Query: 17  EWMSNLPETLTCLPLNYIAIPGXXXXXXXXXXXXXXXAPDAEEIVKKLTSLFGSLAKSII 196
           +WMS LP+T     L  ++IPG                           S  G +  ++ 
Sbjct: 47  QWMSALPDTTN---LAALSIPG----------------------THDTMSYNGDITWTLT 81

Query: 197 ARWAKAQALNTFGQLDSGIRYFDIRVAKNSKDKDEIYSCHSLFGYPIKTDLLNIKDFLDL 376
              A+ Q ++ + QL++GIRY DIR    +KD   IY         +   L  I  FL  
Sbjct: 82  KPLAQTQTMSLYQQLEAGIRYIDIR----AKDNLNIYHGPIFLNASLSGVLETITQFLKK 137

Query: 377 HQKEVVLLDINHFYNFGMNEHYRL 448
           + KE +++ +    N   +  YR+
Sbjct: 138 NPKETIIMRLKDEQNSNDSFDYRI 161
>sp|P45723|PLC_STAAU 1-phosphatidylinositol phosphodiesterase precursor
           (Phosphatidylinositol diacylglycerol-lyase)
           (Phosphatidylinositol-specific phospholipase C) (PI-PLC)
          Length = 311

 Score = 38.9 bits (89), Expect = 0.020
 Identities = 56/262 (21%), Positives = 107/262 (40%), Gaps = 29/262 (11%)
 Frame = +2

Query: 203 WAKAQALNTFGQLDSGIRYFDIRVAKNSKDKDEIYSCHSLFGYPIKTDLLNIKDFLDLHQ 382
           WAK Q  +   Q+ SG+R+FDIR   ++ +   ++       + +   L + K +L  + 
Sbjct: 55  WAKTQDKDYLTQMKSGVRFFDIRGRASADNMISVHHGMVYLHHELGKFLDDAKYYLSAYP 114

Query: 383 KEVVLLDINHFYNFG------MNEHYRLIRHLETVFGNKLMSFSYQIPSLDEIWKTKSRV 544
            E +++ +   Y+          E +R   +    + N   + S   P+L E   TK ++
Sbjct: 115 NETIVMSMKKDYDSDSKVTKTFEEIFREYYYNNPQYQNLFYTGSNANPTLKE---TKGKI 171

Query: 545 IVFYHSNDVQRPYLWPGYFIPS---PWANTDELTKLLDFLTANYK-RGRAIDYF------ 694
           ++F   N +   Y+  GY   +    WA+       ++  + N K +    DY+      
Sbjct: 172 VLF---NRMGGTYIKSGYGADTSGIQWADNATFETKINNGSLNLKVQDEYKDYYDKKVEA 228

Query: 695 --HVSQGVLTPTTGDIVKHVFSSLKSVMADKATPAFIKWLEDKTA------GKGKINCSI 850
             ++   + T +  D V   F S+ S  +D +T  +   +  + A      GK +    I
Sbjct: 229 VKNLLLKLKTDSNKDNVYVNFLSVASGGSDLSTYNYASHINPEIAKTIKANGKARTGWLI 288

Query: 851 VDFAENV-----DYVSRVMQLN 901
           VD+A        D VS ++  N
Sbjct: 289 VDYAGYTWPGYDDIVSEIIDSN 310
>sp|P57225|SYT_BUCAI Threonyl-tRNA synthetase (Threonine--tRNA ligase) (ThrRS)
          Length = 642

 Score = 33.5 bits (75), Expect = 0.84
 Identities = 42/161 (26%), Positives = 71/161 (44%), Gaps = 31/161 (19%)
 Frame = +2

Query: 176 SLAKSIIARWAKAQA--LNTFGQLDSGIRYFDIRVAKNSKDKDEI-YSCHSLFGYPIK-- 340
           S+ KS+IA         LNTF + D+ I + D    KN K  + I YSC  L  Y IK  
Sbjct: 30  SIMKSLIAISVNNHFSNLNTFIREDAFIEFVD---QKNYKALNIIRYSCAQLLSYAIKNI 86

Query: 341 --------TDLLNIKDFLDLH------QKEVVLLDINHFYNFGMNEHYRLIRHLETVFGN 478
                   ++++    + D+       +K+++LL+         N+   L++    +  N
Sbjct: 87  WPLAQIATSNIIEDGFYCDIDFKRSISEKDLILLE---------NQMKMLVKREYNIL-N 136

Query: 479 KLMSFS------------YQIPSLDEIWKTKSRVIVFYHSN 565
           KL+S+S            Y++  +DE     S+V ++YH N
Sbjct: 137 KLISYSEAREIFQKCFEKYKVSLIDENINCNSKVSLYYHEN 177
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 102,645,681
Number of Sequences: 369166
Number of extensions: 2029577
Number of successful extensions: 4900
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 4744
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4895
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9174518830
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)