Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_011_D08
(907 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O15886|PHLC_TRYCR Variant-surface-glycoprotein phospholi... 80 1e-14
sp|P09194|PHLC_TRYBB Variant-surface-glycoprotein phospholi... 74 7e-13
sp|P34024|PLC_LISMO 1-phosphatidylinositol phosphodiesteras... 41 0.004
sp|P45723|PLC_STAAU 1-phosphatidylinositol phosphodiesteras... 39 0.020
sp|P57225|SYT_BUCAI Threonyl-tRNA synthetase (Threonine--tR... 33 0.84
>sp|O15886|PHLC_TRYCR Variant-surface-glycoprotein phospholipase C (VSG lipase)
(Glycosylphosphatidylinositol-specific phospholipase C)
(GPI-PLC)
Length = 380
Score = 79.7 bits (195), Expect = 1e-14
Identities = 68/244 (27%), Positives = 108/244 (44%), Gaps = 30/244 (12%)
Frame = +2
Query: 164 SLFGSLAKSIIARWAKAQALNTFGQLDSGIRYFDIRVAKNSKDKDEIYSCHSLFGYPIKT 343
SL L + I A W++ Q ++ QL+ G+RY D+RVA N +D +Y+ H P+
Sbjct: 69 SLLRFLFRGICASWSRCQWMSVRAQLNHGVRYLDMRVATNPEDASRLYTLHHQISVPLAD 128
Query: 344 DLLNIKDFLD--LHQKEVVLLDINHFY----NFGMNEHYRLIRHLETVFGNKLMSFSYQI 505
L ++K FL+ L E ++LD H Y + G + +R + L F +
Sbjct: 129 VLEDVKAFLNDPLSADEFIVLDFQHLYLTDDSDGKGKFFRELDRLSDRF------IPVDV 182
Query: 506 P---SLDEIWKTKS--RVIVFYHSNDVQRPYLWPGYFIPSP-----WANTDELTKLLDFL 655
P L+ +W+ S R+ + S + + PY P I S W N + L KLL+ L
Sbjct: 183 PLTTPLEFLWRASSRRRIFLVVGSGEDESPY--PAARIRSKCMVSRWVNENSLRKLLEAL 240
Query: 656 ------TANYKRGRAIDYFHVSQGVLTPTTGDIV--------KHVFSSLKSVMADKATPA 793
Y + +V+Q V TP DI + V SS+ V A + P+
Sbjct: 241 DNLLLDDLKYPQTGVPSKLYVTQAVFTPRYSDIFLGIFPKISRRVVSSIYDV-ATRVNPS 299
Query: 794 FIKW 805
++W
Sbjct: 300 LLEW 303
>sp|P09194|PHLC_TRYBB Variant-surface-glycoprotein phospholipase C (VSG lipase)
(Glycosylphosphatidylinositol-specific phospholipase C)
(GPI-PLC)
Length = 358
Score = 73.6 bits (179), Expect = 7e-13
Identities = 69/264 (26%), Positives = 111/264 (42%), Gaps = 26/264 (9%)
Frame = +2
Query: 20 WMSNLPETLTCLPLNYIAIPGXXXXXXXXXXXXXXXAPDAEEIVKKL----TSLFGSLAK 187
WMS+ ++ + + + G DA E ++ L T L L
Sbjct: 12 WMSDTRSSIEKKCIGQVYMVGAHNAGTHGIQMFSPFGLDAPEKLRSLPPYVTFLLRFLTV 71
Query: 188 SIIARWAKAQALNTFGQLDSGIRYFDIRVAKNSKDKDEIYSCHSLFGYPIKTDLLNIKDF 367
+ +RW + Q L+ LD G+RY D+R+ + +++IY+ H P++ L ++KDF
Sbjct: 72 GVSSRWGRCQNLSIRQLLDHGVRYLDLRMNISPDQENKIYTTHFHISVPLQEVLKDVKDF 131
Query: 368 L--DLHQKEVVLLDINHFYNFGMNEHYRLIRHLETVFGNKLMSFSYQIPS-------LDE 520
L E V+LD HFY G NE + + R +E + + + IP+ L
Sbjct: 132 LTTPASANEFVILDFLHFY--GFNESHTMKRFVEELQALE----EFYIPTTVSLTTPLCN 185
Query: 521 IWKTKSRVIVFYHSNDVQRPYLWPGY------FIPSPWANTDELTKLLDFLTANYKR--- 673
+W++ R+ + V RPY+ Y + S W N EL LLD L R
Sbjct: 186 LWQSTRRIFL------VVRPYVEYPYARLRSVALKSIWVNQMELNDLLDRLEELMTRDLE 239
Query: 674 ----GRAIDYFHVSQGVLTPTTGD 733
G +V+Q + TP D
Sbjct: 240 DVSIGGVPSKMYVTQAIGTPRNND 263
>sp|P34024|PLC_LISMO 1-phosphatidylinositol phosphodiesterase precursor
(Phosphatidylinositol diacylglycerol-lyase)
(Phosphatidylinositol-specific phospholipase C) (PI-PLC)
Length = 317
Score = 41.2 bits (95), Expect = 0.004
Identities = 36/144 (25%), Positives = 58/144 (40%)
Frame = +2
Query: 17 EWMSNLPETLTCLPLNYIAIPGXXXXXXXXXXXXXXXAPDAEEIVKKLTSLFGSLAKSII 196
+WMS LP+T L ++IPG S G + ++
Sbjct: 47 QWMSALPDTTN---LAALSIPG----------------------THDTMSYNGDITWTLT 81
Query: 197 ARWAKAQALNTFGQLDSGIRYFDIRVAKNSKDKDEIYSCHSLFGYPIKTDLLNIKDFLDL 376
A+ Q ++ + QL++GIRY DIR +KD IY + L I FL
Sbjct: 82 KPLAQTQTMSLYQQLEAGIRYIDIR----AKDNLNIYHGPIFLNASLSGVLETITQFLKK 137
Query: 377 HQKEVVLLDINHFYNFGMNEHYRL 448
+ KE +++ + N + YR+
Sbjct: 138 NPKETIIMRLKDEQNSNDSFDYRI 161
>sp|P45723|PLC_STAAU 1-phosphatidylinositol phosphodiesterase precursor
(Phosphatidylinositol diacylglycerol-lyase)
(Phosphatidylinositol-specific phospholipase C) (PI-PLC)
Length = 311
Score = 38.9 bits (89), Expect = 0.020
Identities = 56/262 (21%), Positives = 107/262 (40%), Gaps = 29/262 (11%)
Frame = +2
Query: 203 WAKAQALNTFGQLDSGIRYFDIRVAKNSKDKDEIYSCHSLFGYPIKTDLLNIKDFLDLHQ 382
WAK Q + Q+ SG+R+FDIR ++ + ++ + + L + K +L +
Sbjct: 55 WAKTQDKDYLTQMKSGVRFFDIRGRASADNMISVHHGMVYLHHELGKFLDDAKYYLSAYP 114
Query: 383 KEVVLLDINHFYNFG------MNEHYRLIRHLETVFGNKLMSFSYQIPSLDEIWKTKSRV 544
E +++ + Y+ E +R + + N + S P+L E TK ++
Sbjct: 115 NETIVMSMKKDYDSDSKVTKTFEEIFREYYYNNPQYQNLFYTGSNANPTLKE---TKGKI 171
Query: 545 IVFYHSNDVQRPYLWPGYFIPS---PWANTDELTKLLDFLTANYK-RGRAIDYF------ 694
++F N + Y+ GY + WA+ ++ + N K + DY+
Sbjct: 172 VLF---NRMGGTYIKSGYGADTSGIQWADNATFETKINNGSLNLKVQDEYKDYYDKKVEA 228
Query: 695 --HVSQGVLTPTTGDIVKHVFSSLKSVMADKATPAFIKWLEDKTA------GKGKINCSI 850
++ + T + D V F S+ S +D +T + + + A GK + I
Sbjct: 229 VKNLLLKLKTDSNKDNVYVNFLSVASGGSDLSTYNYASHINPEIAKTIKANGKARTGWLI 288
Query: 851 VDFAENV-----DYVSRVMQLN 901
VD+A D VS ++ N
Sbjct: 289 VDYAGYTWPGYDDIVSEIIDSN 310
>sp|P57225|SYT_BUCAI Threonyl-tRNA synthetase (Threonine--tRNA ligase) (ThrRS)
Length = 642
Score = 33.5 bits (75), Expect = 0.84
Identities = 42/161 (26%), Positives = 71/161 (44%), Gaps = 31/161 (19%)
Frame = +2
Query: 176 SLAKSIIARWAKAQA--LNTFGQLDSGIRYFDIRVAKNSKDKDEI-YSCHSLFGYPIK-- 340
S+ KS+IA LNTF + D+ I + D KN K + I YSC L Y IK
Sbjct: 30 SIMKSLIAISVNNHFSNLNTFIREDAFIEFVD---QKNYKALNIIRYSCAQLLSYAIKNI 86
Query: 341 --------TDLLNIKDFLDLH------QKEVVLLDINHFYNFGMNEHYRLIRHLETVFGN 478
++++ + D+ +K+++LL+ N+ L++ + N
Sbjct: 87 WPLAQIATSNIIEDGFYCDIDFKRSISEKDLILLE---------NQMKMLVKREYNIL-N 136
Query: 479 KLMSFS------------YQIPSLDEIWKTKSRVIVFYHSN 565
KL+S+S Y++ +DE S+V ++YH N
Sbjct: 137 KLISYSEAREIFQKCFEKYKVSLIDENINCNSKVSLYYHEN 177
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 102,645,681
Number of Sequences: 369166
Number of extensions: 2029577
Number of successful extensions: 4900
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 4744
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4895
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9174518830
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)