Planaria EST Database


DrC_01693

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01693
         (822 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9Z2A7|DGAT1_MOUSE  Diacylglycerol O-acyltransferase 1 (D...   157   4e-38
sp|Q9GMF1|DGAT1_CERAE  Diacylglycerol O-acyltransferase 1 (D...   155   2e-37
sp|Q9ERM3|DGAT1_RAT  Diacylglycerol O-acyltransferase 1 (Dig...   154   3e-37
sp|O75907|DGAT1_HUMAN  Diacylglycerol O-acyltransferase 1 (D...   153   6e-37
sp|P35610|SOAT1_HUMAN  Sterol O-acyltransferase 1 (Cholester...    54   4e-07
sp|O77760|SOAT1_CERAE  Sterol O-acyltransferase 1 (Cholester...    54   4e-07
sp|O70536|SOAT1_RAT  Sterol O-acyltransferase 1 (Cholesterol...    54   4e-07
sp|Q61263|SOAT1_MOUSE  Sterol O-acyltransferase 1 (Cholester...    54   4e-07
sp|O77761|SOAT1_MACFA  Sterol O-acyltransferase 1 (Cholester...    54   4e-07
sp|Q60457|SOAT1_CRIGR  Sterol O-acyltransferase 1 (Cholester...    54   4e-07
>sp|Q9Z2A7|DGAT1_MOUSE Diacylglycerol O-acyltransferase 1 (Diglyceride acyltransferase)
          Length = 498

 Score =  157 bits (396), Expect = 4e-38
 Identities = 93/249 (37%), Positives = 134/249 (53%), Gaps = 2/249 (0%)
 Frame = +3

Query: 6   FAIEILLEKRHIQDKIGLLFQITTLASMLLYSSYVVLTTHP-SPLISAPVLGIYTVLFLK 182
           F IE  L    + +++GLL  +  LA+++ + + V L     +P+ S   L  Y+++FLK
Sbjct: 157 FQIEKRLAVGALTEQMGLLLHVVNLATIICFPAAVALLVESITPVGSVFALASYSIMFLK 216

Query: 183 LWSYIHVNKWCRDSRSEEVHDKAK-ISFKRNVNGTISTNGLDLAXXXXXXXXXXXXXXXX 359
           L+SY  VN WCR  R      KAK +S  + V+G  +   +                   
Sbjct: 217 LYSYRDVNLWCRQRRV-----KAKAVSTGKKVSGAAAQQAVS------------------ 253

Query: 360 LTSSVDDSLIDSNNLPIMHYQRYPDNLNLFDLYYFIVAPTLCYELNFPRTLKIRKKFLWK 539
                                 YPDNL   DLYYFI APTLCYELNFPR+ +IRK+FL +
Sbjct: 254 ----------------------YPDNLTYRDLYYFIFAPTLCYELNFPRSPRIRKRFLLR 291

Query: 540 RTLELIFLPQLIFCLIQQWILPLLHNSVAPFSQSHFSIVIERMLKLSIPNHLIWVDVLLC 719
           R LE++F  QL   LIQQW++P + NS+ PF    +S +IER+LKL++PNHLIW   L+ 
Sbjct: 292 RVLEMLFFTQLQVGLIQQWMVPTIQNSMKPFKDMDYSRIIERLLKLAVPNHLIW---LIF 348

Query: 720 YFPFWSQCM 746
           ++ F+  C+
Sbjct: 349 FYWFFHSCL 357

 Score = 66.6 bits (161), Expect = 8e-11
 Identities = 30/46 (65%), Positives = 33/46 (71%)
 Frame = +1

Query: 685 PIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
           P  L  L+FFY  FHS LN  AELL FGDR FY+DWWNA +VT FW
Sbjct: 340 PNHLIWLIFFYWFFHSCLNAVAELLQFGDREFYRDWWNAESVTYFW 385
>sp|Q9GMF1|DGAT1_CERAE Diacylglycerol O-acyltransferase 1 (Diglyceride acyltransferase)
          Length = 491

 Score =  155 bits (391), Expect = 2e-37
 Identities = 92/248 (37%), Positives = 134/248 (54%), Gaps = 1/248 (0%)
 Frame = +3

Query: 6   FAIEILLEKRHIQDKIGLLFQITTLASMLLYSSYVVLTTHP-SPLISAPVLGIYTVLFLK 182
           F +E  L    + ++ GLL  +  LA++L + + VVL     +P+ S   L ++T+LFLK
Sbjct: 151 FQVEKRLAVGALTEQAGLLLHVANLATILCFPAAVVLLVESITPVGSLLALMVHTILFLK 210

Query: 183 LWSYIHVNKWCRDSRSEEVHDKAKISFKRNVNGTISTNGLDLAXXXXXXXXXXXXXXXXL 362
           L+SY  VN WCR +R+     KA  + KR                               
Sbjct: 211 LFSYRDVNLWCRRARA-----KAASAGKR------------------------------- 234

Query: 363 TSSVDDSLIDSNNLPIMHYQRYPDNLNLFDLYYFIVAPTLCYELNFPRTLKIRKKFLWKR 542
                     +++    H   YPDNL   DLYYF+ APTLCYELNFPR+ +IRK+FL +R
Sbjct: 235 ----------ASSAAAPHTVSYPDNLTYRDLYYFLFAPTLCYELNFPRSPRIRKRFLLRR 284

Query: 543 TLELIFLPQLIFCLIQQWILPLLHNSVAPFSQSHFSIVIERMLKLSIPNHLIWVDVLLCY 722
            LE++F  QL   LIQQW++P + NS+ PF    +S +IER+LKL++PNHLIW   L+ +
Sbjct: 285 ILEMLFFTQLQVGLIQQWMVPTIQNSMKPFKDMDYSRIIERLLKLAVPNHLIW---LIFF 341

Query: 723 FPFWSQCM 746
           +  +  C+
Sbjct: 342 YWLFHSCL 349

 Score = 65.5 bits (158), Expect = 2e-10
 Identities = 28/46 (60%), Positives = 34/46 (73%)
 Frame = +1

Query: 685 PIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
           P  L  L+FFY +FHS LN  AEL+ FGDR FY+DWWN+ +VT FW
Sbjct: 332 PNHLIWLIFFYWLFHSCLNAVAELMQFGDREFYRDWWNSESVTYFW 377
>sp|Q9ERM3|DGAT1_RAT Diacylglycerol O-acyltransferase 1 (Diglyceride acyltransferase)
          Length = 498

 Score =  154 bits (389), Expect = 3e-37
 Identities = 93/249 (37%), Positives = 133/249 (53%), Gaps = 2/249 (0%)
 Frame = +3

Query: 6   FAIEILLEKRHIQDKIGLLFQITTLASMLLYSSYVVLTTHP-SPLISAPVLGIYTVLFLK 182
           F IE  L    + +++GLL  +  LA+++ + + V L     +P+ S   L  Y+++FLK
Sbjct: 157 FQIEKRLSVGALTEQMGLLLHVVNLATIICFPAAVALLVESITPVGSLFALASYSIIFLK 216

Query: 183 LWSYIHVNKWCRDSRSEEVHDKAK-ISFKRNVNGTISTNGLDLAXXXXXXXXXXXXXXXX 359
           L SY  VN WCR  R      KAK +S  + V+G  + N +                   
Sbjct: 217 LSSYRDVNLWCRQRRV-----KAKAVSAGKKVSGAAAQNTVS------------------ 253

Query: 360 LTSSVDDSLIDSNNLPIMHYQRYPDNLNLFDLYYFIVAPTLCYELNFPRTLKIRKKFLWK 539
                                 YPDNL   DLYYFI APTLCYELNFPR+ +IRK+FL +
Sbjct: 254 ----------------------YPDNLTYRDLYYFIFAPTLCYELNFPRSPRIRKRFLLR 291

Query: 540 RTLELIFLPQLIFCLIQQWILPLLHNSVAPFSQSHFSIVIERMLKLSIPNHLIWVDVLLC 719
           R LE++F  QL   LIQQW++P + NS+ PF    +S +IER+LKL++PNHLIW   L+ 
Sbjct: 292 RVLEMLFFTQLQVGLIQQWMVPTIQNSMKPFKDMDYSRIIERLLKLAVPNHLIW---LIF 348

Query: 720 YFPFWSQCM 746
           ++  +  C+
Sbjct: 349 FYWLFHSCL 357

 Score = 67.4 bits (163), Expect = 5e-11
 Identities = 30/46 (65%), Positives = 34/46 (73%)
 Frame = +1

Query: 685 PIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
           P  L  L+FFY +FHS LN  AELL FGDR FY+DWWNA +VT FW
Sbjct: 340 PNHLIWLIFFYWLFHSCLNAVAELLQFGDREFYRDWWNAESVTYFW 385
>sp|O75907|DGAT1_HUMAN Diacylglycerol O-acyltransferase 1 (Diglyceride acyltransferase)
           (ACAT-related gene product 1)
          Length = 488

 Score =  153 bits (386), Expect = 6e-37
 Identities = 90/248 (36%), Positives = 129/248 (52%), Gaps = 1/248 (0%)
 Frame = +3

Query: 6   FAIEILLEKRHIQDKIGLLFQITTLASMLLYSSYVVLTTHP-SPLISAPVLGIYTVLFLK 182
           F +E  L    + ++ GLL  +  LA++L + + VVL     +P+ S   L  +T+LFLK
Sbjct: 148 FQVEKRLAVGALTEQAGLLLHVANLATILCFPAAVVLLVESITPVGSLLALMAHTILFLK 207

Query: 183 LWSYIHVNKWCRDSRSEEVHDKAKISFKRNVNGTISTNGLDLAXXXXXXXXXXXXXXXXL 362
           L+SY  VN WCR +R++      K S                                  
Sbjct: 208 LFSYRDVNSWCRRARAKAASAGKKAS---------------------------------- 233

Query: 363 TSSVDDSLIDSNNLPIMHYQRYPDNLNLFDLYYFIVAPTLCYELNFPRTLKIRKKFLWKR 542
                       +    H   YPDNL   DLYYF+ APTLCYELNFPR+ +IRK+FL +R
Sbjct: 234 ------------SAAAPHTVSYPDNLTYRDLYYFLFAPTLCYELNFPRSPRIRKRFLLRR 281

Query: 543 TLELIFLPQLIFCLIQQWILPLLHNSVAPFSQSHFSIVIERMLKLSIPNHLIWVDVLLCY 722
            LE++F  QL   LIQQW++P + NS+ PF    +S +IER+LKL++PNHLIW   L+ +
Sbjct: 282 ILEMLFFTQLQVGLIQQWMVPTIQNSMKPFKDMDYSRIIERLLKLAVPNHLIW---LIFF 338

Query: 723 FPFWSQCM 746
           +  +  C+
Sbjct: 339 YWLFHSCL 346

 Score = 65.5 bits (158), Expect = 2e-10
 Identities = 28/46 (60%), Positives = 34/46 (73%)
 Frame = +1

Query: 685 PIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
           P  L  L+FFY +FHS LN  AEL+ FGDR FY+DWWN+ +VT FW
Sbjct: 329 PNHLIWLIFFYWLFHSCLNAVAELMQFGDREFYRDWWNSESVTYFW 374
>sp|P35610|SOAT1_HUMAN Sterol O-acyltransferase 1 (Cholesterol acyltransferase 1) (Acyl
           coenzyme A:cholesterol acyltransferase 1) (ACAT-1)
          Length = 550

 Score = 54.3 bits (129), Expect = 4e-07
 Identities = 22/50 (44%), Positives = 32/50 (64%)
 Frame = +1

Query: 673 NFQFPIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
           N   P +L   + F+A  H  LN  AE+L FGDR+FYKDWWN+ + + ++
Sbjct: 368 NSILPGVLILFLTFFAFLHCWLNAFAEMLRFGDRMFYKDWWNSTSYSNYY 417

 Score = 34.3 bits (77), Expect = 0.42
 Identities = 27/111 (24%), Positives = 52/111 (46%)
 Frame = +3

Query: 459 YFIVAPTLCYELNFPRTLKIRKKFLWKRTLELIFLPQLIFCLIQQWILPLLHNSVAPFSQ 638
           YF+ APTL Y  ++PR   +R  ++  +  ++      ++ + ++   PL  N      Q
Sbjct: 299 YFLFAPTLIYRDSYPRNPTVRWGYVAMKFAQVFGCFFYVYYIFERLCAPLFRN----IKQ 354

Query: 639 SHFSIVIERMLKLSIPNHLIWVDVLLCYFPFWSQCMC*IIVFWRSSFLQRL 791
             FS    R+L L + N ++   VL+ +  F++   C     W ++F + L
Sbjct: 355 EPFS---ARVLVLCVFNSIL-PGVLILFLTFFAFLHC-----WLNAFAEML 396
>sp|O77760|SOAT1_CERAE Sterol O-acyltransferase 1 (Cholesterol acyltransferase 1) (Acyl
           coenzyme A:cholesterol acyltransferase 1) (ACAT-1)
          Length = 550

 Score = 54.3 bits (129), Expect = 4e-07
 Identities = 22/50 (44%), Positives = 32/50 (64%)
 Frame = +1

Query: 673 NFQFPIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
           N   P +L   + F+A  H  LN  AE+L FGDR+FYKDWWN+ + + ++
Sbjct: 368 NSILPGVLILFLTFFAFLHCWLNAFAEMLRFGDRMFYKDWWNSTSYSNYY 417

 Score = 33.9 bits (76), Expect = 0.55
 Identities = 27/111 (24%), Positives = 52/111 (46%)
 Frame = +3

Query: 459 YFIVAPTLCYELNFPRTLKIRKKFLWKRTLELIFLPQLIFCLIQQWILPLLHNSVAPFSQ 638
           YF+ APTL Y  ++PR   +R  ++  +  ++      ++ + ++   PL  N      Q
Sbjct: 299 YFLFAPTLIYRDSYPRNPTVRWGYVAMQFAQVFGCFFYVYYIFERLCAPLFRN----IKQ 354

Query: 639 SHFSIVIERMLKLSIPNHLIWVDVLLCYFPFWSQCMC*IIVFWRSSFLQRL 791
             FS    R+L L + N ++   VL+ +  F++   C     W ++F + L
Sbjct: 355 EPFS---ARVLVLCVFNSIL-PGVLILFLTFFAFLHC-----WLNAFAEML 396
>sp|O70536|SOAT1_RAT Sterol O-acyltransferase 1 (Cholesterol acyltransferase 1) (Acyl
           coenzyme A:cholesterol acyltransferase 1) (ACAT-1)
          Length = 545

 Score = 54.3 bits (129), Expect = 4e-07
 Identities = 22/50 (44%), Positives = 32/50 (64%)
 Frame = +1

Query: 673 NFQFPIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
           N   P +L   + F+A  H  LN  AE+L FGDR+FYKDWWN+ + + ++
Sbjct: 363 NSILPGVLMLFLSFFAFLHCWLNAFAEMLRFGDRMFYKDWWNSTSYSNYY 412

 Score = 38.1 bits (87), Expect = 0.029
 Identities = 29/111 (26%), Positives = 54/111 (48%)
 Frame = +3

Query: 459 YFIVAPTLCYELNFPRTLKIRKKFLWKRTLELIFLPQLIFCLIQQWILPLLHNSVAPFSQ 638
           YF+ APTL Y  ++PRT  +R  ++  + L++      ++ + ++   PL  N      Q
Sbjct: 294 YFLFAPTLIYRDSYPRTPTVRWGYVAMQFLQVFGCLFYVYYIFERLCAPLFRN----IKQ 349

Query: 639 SHFSIVIERMLKLSIPNHLIWVDVLLCYFPFWSQCMC*IIVFWRSSFLQRL 791
             FS    R+L L + N ++   VL+ +  F++   C     W ++F + L
Sbjct: 350 EPFS---ARVLVLCVFNSIL-PGVLMLFLSFFAFLHC-----WLNAFAEML 391
>sp|Q61263|SOAT1_MOUSE Sterol O-acyltransferase 1 (Cholesterol acyltransferase 1) (Acyl
           coenzyme A:cholesterol acyltransferase 1) (ACAT-1)
          Length = 540

 Score = 54.3 bits (129), Expect = 4e-07
 Identities = 22/50 (44%), Positives = 32/50 (64%)
 Frame = +1

Query: 673 NFQFPIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
           N   P +L   + F+A  H  LN  AE+L FGDR+FYKDWWN+ + + ++
Sbjct: 358 NSILPGVLILFLSFFAFLHCWLNAFAEMLRFGDRMFYKDWWNSTSYSNYY 407

 Score = 40.0 bits (92), Expect = 0.008
 Identities = 30/111 (27%), Positives = 54/111 (48%)
 Frame = +3

Query: 459 YFIVAPTLCYELNFPRTLKIRKKFLWKRTLELIFLPQLIFCLIQQWILPLLHNSVAPFSQ 638
           YF+ APTL Y  N+PRT  +R  ++  + L++      ++ + ++   PL  N      Q
Sbjct: 289 YFLFAPTLIYRDNYPRTPTVRWGYVAMQFLQVFGCLFYVYYIFERLCAPLFRN----IKQ 344

Query: 639 SHFSIVIERMLKLSIPNHLIWVDVLLCYFPFWSQCMC*IIVFWRSSFLQRL 791
             FS    R+L L + N ++   VL+ +  F++   C     W ++F + L
Sbjct: 345 EPFS---ARVLVLCVFNSIL-PGVLILFLSFFAFLHC-----WLNAFAEML 386
>sp|O77761|SOAT1_MACFA Sterol O-acyltransferase 1 (Cholesterol acyltransferase 1) (Acyl
           coenzyme A:cholesterol acyltransferase 1) (ACAT-1)
          Length = 550

 Score = 54.3 bits (129), Expect = 4e-07
 Identities = 22/50 (44%), Positives = 32/50 (64%)
 Frame = +1

Query: 673 NFQFPIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
           N   P +L   + F+A  H  LN  AE+L FGDR+FYKDWWN+ + + ++
Sbjct: 368 NSILPGVLILFLTFFAFLHCWLNAFAEMLRFGDRMFYKDWWNSTSYSNYY 417

 Score = 33.9 bits (76), Expect = 0.55
 Identities = 27/111 (24%), Positives = 52/111 (46%)
 Frame = +3

Query: 459 YFIVAPTLCYELNFPRTLKIRKKFLWKRTLELIFLPQLIFCLIQQWILPLLHNSVAPFSQ 638
           YF+ APTL Y  ++PR   +R  ++  +  ++      ++ + ++   PL  N      Q
Sbjct: 299 YFLFAPTLIYRDSYPRNPTVRWGYVAMQFAQVFGCFFYVYYIFERLCAPLFRN----IKQ 354

Query: 639 SHFSIVIERMLKLSIPNHLIWVDVLLCYFPFWSQCMC*IIVFWRSSFLQRL 791
             FS    R+L L + N ++   VL+ +  F++   C     W ++F + L
Sbjct: 355 EPFS---ARVLVLCVFNSIL-PGVLILFLTFFAFLHC-----WLNAFAEML 396
>sp|Q60457|SOAT1_CRIGR Sterol O-acyltransferase 1 (Cholesterol acyltransferase 1) (Acyl
           coenzyme A:cholesterol acyltransferase 1) (ACAT-1)
          Length = 546

 Score = 54.3 bits (129), Expect = 4e-07
 Identities = 22/50 (44%), Positives = 32/50 (64%)
 Frame = +1

Query: 673 NFQFPIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
           N   P +L   + F+A  H  LN  AE+L FGDR+FYKDWWN+ + + ++
Sbjct: 364 NSILPGVLMLFLTFFAFLHCWLNAFAEMLRFGDRMFYKDWWNSTSYSNYY 413

 Score = 37.7 bits (86), Expect = 0.038
 Identities = 29/111 (26%), Positives = 54/111 (48%)
 Frame = +3

Query: 459 YFIVAPTLCYELNFPRTLKIRKKFLWKRTLELIFLPQLIFCLIQQWILPLLHNSVAPFSQ 638
           YF+ APTL Y  ++PRT  +R  ++  + L++      ++ + ++   PL  N      Q
Sbjct: 295 YFLFAPTLIYRDSYPRTPTVRWGYVAVQFLQVFGCLFYVYYIFERLCAPLFRN----IKQ 350

Query: 639 SHFSIVIERMLKLSIPNHLIWVDVLLCYFPFWSQCMC*IIVFWRSSFLQRL 791
             FS    R+L L + N ++   VL+ +  F++   C     W ++F + L
Sbjct: 351 EPFS---ARVLVLCVFNSIL-PGVLMLFLTFFAFLHC-----WLNAFAEML 392
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 81,358,314
Number of Sequences: 369166
Number of extensions: 1451880
Number of successful extensions: 3188
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3100
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3185
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7907893860
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)