Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_008_M18
(822 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9Z2A7|DGAT1_MOUSE Diacylglycerol O-acyltransferase 1 (D... 157 4e-38
sp|Q9GMF1|DGAT1_CERAE Diacylglycerol O-acyltransferase 1 (D... 155 2e-37
sp|Q9ERM3|DGAT1_RAT Diacylglycerol O-acyltransferase 1 (Dig... 154 3e-37
sp|O75907|DGAT1_HUMAN Diacylglycerol O-acyltransferase 1 (D... 153 6e-37
sp|P35610|SOAT1_HUMAN Sterol O-acyltransferase 1 (Cholester... 54 4e-07
sp|O77760|SOAT1_CERAE Sterol O-acyltransferase 1 (Cholester... 54 4e-07
sp|O70536|SOAT1_RAT Sterol O-acyltransferase 1 (Cholesterol... 54 4e-07
sp|Q61263|SOAT1_MOUSE Sterol O-acyltransferase 1 (Cholester... 54 4e-07
sp|O77761|SOAT1_MACFA Sterol O-acyltransferase 1 (Cholester... 54 4e-07
sp|Q60457|SOAT1_CRIGR Sterol O-acyltransferase 1 (Cholester... 54 4e-07
>sp|Q9Z2A7|DGAT1_MOUSE Diacylglycerol O-acyltransferase 1 (Diglyceride acyltransferase)
Length = 498
Score = 157 bits (396), Expect = 4e-38
Identities = 93/249 (37%), Positives = 134/249 (53%), Gaps = 2/249 (0%)
Frame = +3
Query: 6 FAIEILLEKRHIQDKIGLLFQITTLASMLLYSSYVVLTTHP-SPLISAPVLGIYTVLFLK 182
F IE L + +++GLL + LA+++ + + V L +P+ S L Y+++FLK
Sbjct: 157 FQIEKRLAVGALTEQMGLLLHVVNLATIICFPAAVALLVESITPVGSVFALASYSIMFLK 216
Query: 183 LWSYIHVNKWCRDSRSEEVHDKAK-ISFKRNVNGTISTNGLDLAXXXXXXXXXXXXXXXX 359
L+SY VN WCR R KAK +S + V+G + +
Sbjct: 217 LYSYRDVNLWCRQRRV-----KAKAVSTGKKVSGAAAQQAVS------------------ 253
Query: 360 LTSSVDDSLIDSNNLPIMHYQRYPDNLNLFDLYYFIVAPTLCYELNFPRTLKIRKKFLWK 539
YPDNL DLYYFI APTLCYELNFPR+ +IRK+FL +
Sbjct: 254 ----------------------YPDNLTYRDLYYFIFAPTLCYELNFPRSPRIRKRFLLR 291
Query: 540 RTLELIFLPQLIFCLIQQWILPLLHNSVAPFSQSHFSIVIERMLKLSIPNHLIWVDVLLC 719
R LE++F QL LIQQW++P + NS+ PF +S +IER+LKL++PNHLIW L+
Sbjct: 292 RVLEMLFFTQLQVGLIQQWMVPTIQNSMKPFKDMDYSRIIERLLKLAVPNHLIW---LIF 348
Query: 720 YFPFWSQCM 746
++ F+ C+
Sbjct: 349 FYWFFHSCL 357
Score = 66.6 bits (161), Expect = 8e-11
Identities = 30/46 (65%), Positives = 33/46 (71%)
Frame = +1
Query: 685 PIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
P L L+FFY FHS LN AELL FGDR FY+DWWNA +VT FW
Sbjct: 340 PNHLIWLIFFYWFFHSCLNAVAELLQFGDREFYRDWWNAESVTYFW 385
>sp|Q9GMF1|DGAT1_CERAE Diacylglycerol O-acyltransferase 1 (Diglyceride acyltransferase)
Length = 491
Score = 155 bits (391), Expect = 2e-37
Identities = 92/248 (37%), Positives = 134/248 (54%), Gaps = 1/248 (0%)
Frame = +3
Query: 6 FAIEILLEKRHIQDKIGLLFQITTLASMLLYSSYVVLTTHP-SPLISAPVLGIYTVLFLK 182
F +E L + ++ GLL + LA++L + + VVL +P+ S L ++T+LFLK
Sbjct: 151 FQVEKRLAVGALTEQAGLLLHVANLATILCFPAAVVLLVESITPVGSLLALMVHTILFLK 210
Query: 183 LWSYIHVNKWCRDSRSEEVHDKAKISFKRNVNGTISTNGLDLAXXXXXXXXXXXXXXXXL 362
L+SY VN WCR +R+ KA + KR
Sbjct: 211 LFSYRDVNLWCRRARA-----KAASAGKR------------------------------- 234
Query: 363 TSSVDDSLIDSNNLPIMHYQRYPDNLNLFDLYYFIVAPTLCYELNFPRTLKIRKKFLWKR 542
+++ H YPDNL DLYYF+ APTLCYELNFPR+ +IRK+FL +R
Sbjct: 235 ----------ASSAAAPHTVSYPDNLTYRDLYYFLFAPTLCYELNFPRSPRIRKRFLLRR 284
Query: 543 TLELIFLPQLIFCLIQQWILPLLHNSVAPFSQSHFSIVIERMLKLSIPNHLIWVDVLLCY 722
LE++F QL LIQQW++P + NS+ PF +S +IER+LKL++PNHLIW L+ +
Sbjct: 285 ILEMLFFTQLQVGLIQQWMVPTIQNSMKPFKDMDYSRIIERLLKLAVPNHLIW---LIFF 341
Query: 723 FPFWSQCM 746
+ + C+
Sbjct: 342 YWLFHSCL 349
Score = 65.5 bits (158), Expect = 2e-10
Identities = 28/46 (60%), Positives = 34/46 (73%)
Frame = +1
Query: 685 PIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
P L L+FFY +FHS LN AEL+ FGDR FY+DWWN+ +VT FW
Sbjct: 332 PNHLIWLIFFYWLFHSCLNAVAELMQFGDREFYRDWWNSESVTYFW 377
>sp|Q9ERM3|DGAT1_RAT Diacylglycerol O-acyltransferase 1 (Diglyceride acyltransferase)
Length = 498
Score = 154 bits (389), Expect = 3e-37
Identities = 93/249 (37%), Positives = 133/249 (53%), Gaps = 2/249 (0%)
Frame = +3
Query: 6 FAIEILLEKRHIQDKIGLLFQITTLASMLLYSSYVVLTTHP-SPLISAPVLGIYTVLFLK 182
F IE L + +++GLL + LA+++ + + V L +P+ S L Y+++FLK
Sbjct: 157 FQIEKRLSVGALTEQMGLLLHVVNLATIICFPAAVALLVESITPVGSLFALASYSIIFLK 216
Query: 183 LWSYIHVNKWCRDSRSEEVHDKAK-ISFKRNVNGTISTNGLDLAXXXXXXXXXXXXXXXX 359
L SY VN WCR R KAK +S + V+G + N +
Sbjct: 217 LSSYRDVNLWCRQRRV-----KAKAVSAGKKVSGAAAQNTVS------------------ 253
Query: 360 LTSSVDDSLIDSNNLPIMHYQRYPDNLNLFDLYYFIVAPTLCYELNFPRTLKIRKKFLWK 539
YPDNL DLYYFI APTLCYELNFPR+ +IRK+FL +
Sbjct: 254 ----------------------YPDNLTYRDLYYFIFAPTLCYELNFPRSPRIRKRFLLR 291
Query: 540 RTLELIFLPQLIFCLIQQWILPLLHNSVAPFSQSHFSIVIERMLKLSIPNHLIWVDVLLC 719
R LE++F QL LIQQW++P + NS+ PF +S +IER+LKL++PNHLIW L+
Sbjct: 292 RVLEMLFFTQLQVGLIQQWMVPTIQNSMKPFKDMDYSRIIERLLKLAVPNHLIW---LIF 348
Query: 720 YFPFWSQCM 746
++ + C+
Sbjct: 349 FYWLFHSCL 357
Score = 67.4 bits (163), Expect = 5e-11
Identities = 30/46 (65%), Positives = 34/46 (73%)
Frame = +1
Query: 685 PIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
P L L+FFY +FHS LN AELL FGDR FY+DWWNA +VT FW
Sbjct: 340 PNHLIWLIFFYWLFHSCLNAVAELLQFGDREFYRDWWNAESVTYFW 385
>sp|O75907|DGAT1_HUMAN Diacylglycerol O-acyltransferase 1 (Diglyceride acyltransferase)
(ACAT-related gene product 1)
Length = 488
Score = 153 bits (386), Expect = 6e-37
Identities = 90/248 (36%), Positives = 129/248 (52%), Gaps = 1/248 (0%)
Frame = +3
Query: 6 FAIEILLEKRHIQDKIGLLFQITTLASMLLYSSYVVLTTHP-SPLISAPVLGIYTVLFLK 182
F +E L + ++ GLL + LA++L + + VVL +P+ S L +T+LFLK
Sbjct: 148 FQVEKRLAVGALTEQAGLLLHVANLATILCFPAAVVLLVESITPVGSLLALMAHTILFLK 207
Query: 183 LWSYIHVNKWCRDSRSEEVHDKAKISFKRNVNGTISTNGLDLAXXXXXXXXXXXXXXXXL 362
L+SY VN WCR +R++ K S
Sbjct: 208 LFSYRDVNSWCRRARAKAASAGKKAS---------------------------------- 233
Query: 363 TSSVDDSLIDSNNLPIMHYQRYPDNLNLFDLYYFIVAPTLCYELNFPRTLKIRKKFLWKR 542
+ H YPDNL DLYYF+ APTLCYELNFPR+ +IRK+FL +R
Sbjct: 234 ------------SAAAPHTVSYPDNLTYRDLYYFLFAPTLCYELNFPRSPRIRKRFLLRR 281
Query: 543 TLELIFLPQLIFCLIQQWILPLLHNSVAPFSQSHFSIVIERMLKLSIPNHLIWVDVLLCY 722
LE++F QL LIQQW++P + NS+ PF +S +IER+LKL++PNHLIW L+ +
Sbjct: 282 ILEMLFFTQLQVGLIQQWMVPTIQNSMKPFKDMDYSRIIERLLKLAVPNHLIW---LIFF 338
Query: 723 FPFWSQCM 746
+ + C+
Sbjct: 339 YWLFHSCL 346
Score = 65.5 bits (158), Expect = 2e-10
Identities = 28/46 (60%), Positives = 34/46 (73%)
Frame = +1
Query: 685 PIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
P L L+FFY +FHS LN AEL+ FGDR FY+DWWN+ +VT FW
Sbjct: 329 PNHLIWLIFFYWLFHSCLNAVAELMQFGDREFYRDWWNSESVTYFW 374
>sp|P35610|SOAT1_HUMAN Sterol O-acyltransferase 1 (Cholesterol acyltransferase 1) (Acyl
coenzyme A:cholesterol acyltransferase 1) (ACAT-1)
Length = 550
Score = 54.3 bits (129), Expect = 4e-07
Identities = 22/50 (44%), Positives = 32/50 (64%)
Frame = +1
Query: 673 NFQFPIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
N P +L + F+A H LN AE+L FGDR+FYKDWWN+ + + ++
Sbjct: 368 NSILPGVLILFLTFFAFLHCWLNAFAEMLRFGDRMFYKDWWNSTSYSNYY 417
Score = 34.3 bits (77), Expect = 0.42
Identities = 27/111 (24%), Positives = 52/111 (46%)
Frame = +3
Query: 459 YFIVAPTLCYELNFPRTLKIRKKFLWKRTLELIFLPQLIFCLIQQWILPLLHNSVAPFSQ 638
YF+ APTL Y ++PR +R ++ + ++ ++ + ++ PL N Q
Sbjct: 299 YFLFAPTLIYRDSYPRNPTVRWGYVAMKFAQVFGCFFYVYYIFERLCAPLFRN----IKQ 354
Query: 639 SHFSIVIERMLKLSIPNHLIWVDVLLCYFPFWSQCMC*IIVFWRSSFLQRL 791
FS R+L L + N ++ VL+ + F++ C W ++F + L
Sbjct: 355 EPFS---ARVLVLCVFNSIL-PGVLILFLTFFAFLHC-----WLNAFAEML 396
>sp|O77760|SOAT1_CERAE Sterol O-acyltransferase 1 (Cholesterol acyltransferase 1) (Acyl
coenzyme A:cholesterol acyltransferase 1) (ACAT-1)
Length = 550
Score = 54.3 bits (129), Expect = 4e-07
Identities = 22/50 (44%), Positives = 32/50 (64%)
Frame = +1
Query: 673 NFQFPIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
N P +L + F+A H LN AE+L FGDR+FYKDWWN+ + + ++
Sbjct: 368 NSILPGVLILFLTFFAFLHCWLNAFAEMLRFGDRMFYKDWWNSTSYSNYY 417
Score = 33.9 bits (76), Expect = 0.55
Identities = 27/111 (24%), Positives = 52/111 (46%)
Frame = +3
Query: 459 YFIVAPTLCYELNFPRTLKIRKKFLWKRTLELIFLPQLIFCLIQQWILPLLHNSVAPFSQ 638
YF+ APTL Y ++PR +R ++ + ++ ++ + ++ PL N Q
Sbjct: 299 YFLFAPTLIYRDSYPRNPTVRWGYVAMQFAQVFGCFFYVYYIFERLCAPLFRN----IKQ 354
Query: 639 SHFSIVIERMLKLSIPNHLIWVDVLLCYFPFWSQCMC*IIVFWRSSFLQRL 791
FS R+L L + N ++ VL+ + F++ C W ++F + L
Sbjct: 355 EPFS---ARVLVLCVFNSIL-PGVLILFLTFFAFLHC-----WLNAFAEML 396
>sp|O70536|SOAT1_RAT Sterol O-acyltransferase 1 (Cholesterol acyltransferase 1) (Acyl
coenzyme A:cholesterol acyltransferase 1) (ACAT-1)
Length = 545
Score = 54.3 bits (129), Expect = 4e-07
Identities = 22/50 (44%), Positives = 32/50 (64%)
Frame = +1
Query: 673 NFQFPIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
N P +L + F+A H LN AE+L FGDR+FYKDWWN+ + + ++
Sbjct: 363 NSILPGVLMLFLSFFAFLHCWLNAFAEMLRFGDRMFYKDWWNSTSYSNYY 412
Score = 38.1 bits (87), Expect = 0.029
Identities = 29/111 (26%), Positives = 54/111 (48%)
Frame = +3
Query: 459 YFIVAPTLCYELNFPRTLKIRKKFLWKRTLELIFLPQLIFCLIQQWILPLLHNSVAPFSQ 638
YF+ APTL Y ++PRT +R ++ + L++ ++ + ++ PL N Q
Sbjct: 294 YFLFAPTLIYRDSYPRTPTVRWGYVAMQFLQVFGCLFYVYYIFERLCAPLFRN----IKQ 349
Query: 639 SHFSIVIERMLKLSIPNHLIWVDVLLCYFPFWSQCMC*IIVFWRSSFLQRL 791
FS R+L L + N ++ VL+ + F++ C W ++F + L
Sbjct: 350 EPFS---ARVLVLCVFNSIL-PGVLMLFLSFFAFLHC-----WLNAFAEML 391
>sp|Q61263|SOAT1_MOUSE Sterol O-acyltransferase 1 (Cholesterol acyltransferase 1) (Acyl
coenzyme A:cholesterol acyltransferase 1) (ACAT-1)
Length = 540
Score = 54.3 bits (129), Expect = 4e-07
Identities = 22/50 (44%), Positives = 32/50 (64%)
Frame = +1
Query: 673 NFQFPIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
N P +L + F+A H LN AE+L FGDR+FYKDWWN+ + + ++
Sbjct: 358 NSILPGVLILFLSFFAFLHCWLNAFAEMLRFGDRMFYKDWWNSTSYSNYY 407
Score = 40.0 bits (92), Expect = 0.008
Identities = 30/111 (27%), Positives = 54/111 (48%)
Frame = +3
Query: 459 YFIVAPTLCYELNFPRTLKIRKKFLWKRTLELIFLPQLIFCLIQQWILPLLHNSVAPFSQ 638
YF+ APTL Y N+PRT +R ++ + L++ ++ + ++ PL N Q
Sbjct: 289 YFLFAPTLIYRDNYPRTPTVRWGYVAMQFLQVFGCLFYVYYIFERLCAPLFRN----IKQ 344
Query: 639 SHFSIVIERMLKLSIPNHLIWVDVLLCYFPFWSQCMC*IIVFWRSSFLQRL 791
FS R+L L + N ++ VL+ + F++ C W ++F + L
Sbjct: 345 EPFS---ARVLVLCVFNSIL-PGVLILFLSFFAFLHC-----WLNAFAEML 386
>sp|O77761|SOAT1_MACFA Sterol O-acyltransferase 1 (Cholesterol acyltransferase 1) (Acyl
coenzyme A:cholesterol acyltransferase 1) (ACAT-1)
Length = 550
Score = 54.3 bits (129), Expect = 4e-07
Identities = 22/50 (44%), Positives = 32/50 (64%)
Frame = +1
Query: 673 NFQFPIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
N P +L + F+A H LN AE+L FGDR+FYKDWWN+ + + ++
Sbjct: 368 NSILPGVLILFLTFFAFLHCWLNAFAEMLRFGDRMFYKDWWNSTSYSNYY 417
Score = 33.9 bits (76), Expect = 0.55
Identities = 27/111 (24%), Positives = 52/111 (46%)
Frame = +3
Query: 459 YFIVAPTLCYELNFPRTLKIRKKFLWKRTLELIFLPQLIFCLIQQWILPLLHNSVAPFSQ 638
YF+ APTL Y ++PR +R ++ + ++ ++ + ++ PL N Q
Sbjct: 299 YFLFAPTLIYRDSYPRNPTVRWGYVAMQFAQVFGCFFYVYYIFERLCAPLFRN----IKQ 354
Query: 639 SHFSIVIERMLKLSIPNHLIWVDVLLCYFPFWSQCMC*IIVFWRSSFLQRL 791
FS R+L L + N ++ VL+ + F++ C W ++F + L
Sbjct: 355 EPFS---ARVLVLCVFNSIL-PGVLILFLTFFAFLHC-----WLNAFAEML 396
>sp|Q60457|SOAT1_CRIGR Sterol O-acyltransferase 1 (Cholesterol acyltransferase 1) (Acyl
coenzyme A:cholesterol acyltransferase 1) (ACAT-1)
Length = 546
Score = 54.3 bits (129), Expect = 4e-07
Identities = 22/50 (44%), Positives = 32/50 (64%)
Frame = +1
Query: 673 NFQFPIILYGLMFFYAIFHSGLNVCAELLYFGDRLFYKDWWNANTVTEFW 822
N P +L + F+A H LN AE+L FGDR+FYKDWWN+ + + ++
Sbjct: 364 NSILPGVLMLFLTFFAFLHCWLNAFAEMLRFGDRMFYKDWWNSTSYSNYY 413
Score = 37.7 bits (86), Expect = 0.038
Identities = 29/111 (26%), Positives = 54/111 (48%)
Frame = +3
Query: 459 YFIVAPTLCYELNFPRTLKIRKKFLWKRTLELIFLPQLIFCLIQQWILPLLHNSVAPFSQ 638
YF+ APTL Y ++PRT +R ++ + L++ ++ + ++ PL N Q
Sbjct: 295 YFLFAPTLIYRDSYPRTPTVRWGYVAVQFLQVFGCLFYVYYIFERLCAPLFRN----IKQ 350
Query: 639 SHFSIVIERMLKLSIPNHLIWVDVLLCYFPFWSQCMC*IIVFWRSSFLQRL 791
FS R+L L + N ++ VL+ + F++ C W ++F + L
Sbjct: 351 EPFS---ARVLVLCVFNSIL-PGVLMLFLTFFAFLHC-----WLNAFAEML 392
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 81,358,314
Number of Sequences: 369166
Number of extensions: 1451880
Number of successful extensions: 3188
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3100
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3185
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7907893860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)