Planaria EST Database


DrC_01495

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01495
         (367 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P10733|SEVE_DICDI  Severin                                      65   4e-11
sp|Q24800|SEVE_ECHGR  Severin                                      59   3e-09
sp|Q27319|GELS_HOMAM  Gelsolin, cytoplasmic (Actin-depolymer...    50   2e-06
sp|Q07171|GELS_DROME  Gelsolin precursor                           49   5e-06
sp|P09327|VILI_HUMAN  Villin-1                                     45   7e-05
sp|Q62468|VILI_MOUSE  Villin-1                                     44   2e-04
sp|Q60604|ADSV_MOUSE  Adseverin (Scinderin) (Gelsolin-like p...    43   3e-04
sp|P40121|CAPG_HUMAN  Macrophage capping protein (Actin-regu...    42   4e-04
sp|P14885|GELS_XENLA  Gelsolin (Actin-depolymerizing factor)...    42   6e-04
sp|O81645|VILI3_ARATH  Villin-3                                    40   0.001
>sp|P10733|SEVE_DICDI Severin
          Length = 362

 Score = 65.5 bits (158), Expect = 4e-11
 Identities = 32/87 (36%), Positives = 53/87 (60%)
 Frame = +2

Query: 20  ATGPLKFEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKT 199
           A+G LKF +V+ G +++S +  +DVFI+D G  IY ++G  SSPNE+K A  +A  YL  
Sbjct: 276 ASGSLKFSEVSRGKINKSSLKSEDVFIIDLGNEIYTWIGSKSSPNEKKTAFSHATQYLVN 335

Query: 200 TEYPQAPVTVINKNVKGKTAENFNNLV 280
            +  +   T I + ++  T ++F  L+
Sbjct: 336 NK--RCEYTPIVRVLENGTNQSFETLL 360

 Score = 38.9 bits (89), Expect = 0.004
 Identities = 19/45 (42%), Positives = 26/45 (57%)
 Frame = +2

Query: 35  KFEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNA 169
           K  KVAE  ++ S +N  D F++D G  IY + G  SSP E+  A
Sbjct: 170 KNAKVAEVPLATSSLNSGDCFLLDAGLTIYQFNGSKSSPQEKNKA 214
>sp|Q24800|SEVE_ECHGR Severin
          Length = 374

 Score = 59.3 bits (142), Expect = 3e-09
 Identities = 27/71 (38%), Positives = 47/71 (66%)
 Frame = +2

Query: 20  ATGPLKFEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKT 199
           ++G L+   V+EG+ S+  I  DDV+I+   + ++VY+G++ S  E++NAL  AH +L+T
Sbjct: 289 SSGKLELTIVSEGSASKHDIKPDDVYIILTKEGLFVYIGKDCSVLEKRNALSNAHKFLQT 348

Query: 200 TEYPQAPVTVI 232
              P  P+TV+
Sbjct: 349 CPNPFLPITVV 359
>sp|Q27319|GELS_HOMAM Gelsolin, cytoplasmic (Actin-depolymerizing factor) (ADF)
          Length = 754

 Score = 49.7 bits (117), Expect = 2e-06
 Identities = 24/72 (33%), Positives = 42/72 (58%), Gaps = 1/72 (1%)
 Frame = +2

Query: 65  SRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKTTEYPQA-PVTVINKN 241
           ++  +NEDDV ++D+G  +YV+VG+ S   E++ A   A NY+KT    +    TVI + 
Sbjct: 652 AQEDLNEDDVMVLDSGDEVYVWVGQGSDDQEKEKAFTMAENYIKTDPTERTLDATVILRI 711

Query: 242 VKGKTAENFNNL 277
            +G+    F ++
Sbjct: 712 NQGEEPAAFTSI 723

 Score = 40.4 bits (93), Expect = 0.001
 Identities = 17/62 (27%), Positives = 34/62 (54%)
 Frame = +2

Query: 26  GPLKFEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKTTE 205
           G ++   + E  + +S ++  D F++D G  +YV++G  SS  E+  ++  A  Y++   
Sbjct: 261 GVIQTNMIGEKPLLQSMLDSGDCFLLDTGVGVYVWIGSGSSKKEKVKSMELAAGYMEKKG 320

Query: 206 YP 211
           YP
Sbjct: 321 YP 322

 Score = 28.9 bits (63), Expect = 3.9
 Identities = 14/51 (27%), Positives = 27/51 (52%)
 Frame = +2

Query: 8   FGTRATGPLKFEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNER 160
           F  R T       + +  V+ S +N DDVF+++     Y+++G+ +S  E+
Sbjct: 530 FRVRGTCDFDTRAIQQTEVAGS-LNSDDVFVLETPGKTYLWIGKGASEEEK 579
>sp|Q07171|GELS_DROME Gelsolin precursor
          Length = 798

 Score = 48.5 bits (114), Expect = 5e-06
 Identities = 24/83 (28%), Positives = 51/83 (61%)
 Frame = +2

Query: 20  ATGPLKFEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKT 199
           A+G LK + + +  ++++ ++  + FI+D G  I+V+VG+ ++  E+ +A+  A  +L+T
Sbjct: 318 ASGKLKVDIIGQKPLTQAMLDTRECFILDTGSGIFVWVGKGATQKEKTDAMAKAQEFLRT 377

Query: 200 TEYPQAPVTVINKNVKGKTAENF 268
            +YP    T I++ V+G  +  F
Sbjct: 378 KKYP--AWTQIHRIVEGSESAPF 398

 Score = 38.1 bits (87), Expect = 0.006
 Identities = 21/64 (32%), Positives = 34/64 (53%)
 Frame = +2

Query: 44  KVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKTTEYPQAPV 223
           +V + N+S S +N  D FI+D G  IYVYVG  +   E+  A+  A+         +A V
Sbjct: 204 RVRQVNLSVSSMNTGDCFILDAGSDIYVYVGSQAKRVEKLKAISAANQIRDQDHNGRARV 263

Query: 224 TVIN 235
            +++
Sbjct: 264 QIVD 267

 Score = 32.7 bits (73), Expect = 0.27
 Identities = 19/55 (34%), Positives = 29/55 (52%)
 Frame = +2

Query: 26  GPLKFEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNY 190
           G LK E+VA+    +  ++ DD+ ++D G  IY++VG   S  E    L  A  Y
Sbjct: 687 GFLKVEEVAQ--YEQEDLDSDDIMLLDAGDEIYLWVGYGVSEEENGKLLDTAKLY 739
>sp|P09327|VILI_HUMAN Villin-1
          Length = 827

 Score = 44.7 bits (104), Expect = 7e-05
 Identities = 25/88 (28%), Positives = 49/88 (55%), Gaps = 1/88 (1%)
 Frame = +2

Query: 26  GPLKFEKVAEGNVSRSQINEDDVFIVDNGQV-IYVYVGENSSPNERKNALIYAHNYLKTT 202
           G L   +VA   +++  ++ +D +I+D G + IYV+ G+ ++  E+K A+ +A N++K  
Sbjct: 263 GNLVVREVATRPLTQDLLSHEDCYILDQGGLKIYVWKGKKANEQEKKGAMSHALNFIKAK 322

Query: 203 EYPQAPVTVINKNVKGKTAENFNNLVKE 286
           +YP  P T +     G  +  F  L ++
Sbjct: 323 QYP--PSTQVEVQNDGAESAVFQQLFQK 348

 Score = 40.8 bits (94), Expect = 0.001
 Identities = 18/61 (29%), Positives = 36/61 (59%), Gaps = 3/61 (4%)
 Frame = +2

Query: 65  SRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKT---TEYPQAPVTVIN 235
           ++  + EDDVF++D    ++ ++G++++  E+K A   A  YLKT      P+ P+ V+ 
Sbjct: 640 NQDDLEEDDVFLLDVWDQVFFWIGKHANEEEKKAAATTAQEYLKTHPSGRDPETPIIVVK 699

Query: 236 K 238
           +
Sbjct: 700 Q 700
>sp|Q62468|VILI_MOUSE Villin-1
          Length = 827

 Score = 43.5 bits (101), Expect = 2e-04
 Identities = 25/88 (28%), Positives = 47/88 (53%), Gaps = 1/88 (1%)
 Frame = +2

Query: 26  GPLKFEKVAEGNVSRSQINEDDVFIVDNGQV-IYVYVGENSSPNERKNALIYAHNYLKTT 202
           G L   +VA   +++  +  +D +I+D G + I+V+ G+N++  ER  A+  A N++K  
Sbjct: 263 GKLVVREVATRPLTQDLLKHEDCYILDQGGLKIFVWKGKNANAQERSGAMSQALNFIKAK 322

Query: 203 EYPQAPVTVINKNVKGKTAENFNNLVKE 286
           +YP  P T +     G  +  F  L ++
Sbjct: 323 QYP--PSTQVEVQNDGAESPIFQQLFQK 348

 Score = 35.0 bits (79), Expect = 0.054
 Identities = 13/45 (28%), Positives = 28/45 (62%)
 Frame = +2

Query: 65  SRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKT 199
           ++  + E+DVF++D    ++ ++G++++  E+K A      YLKT
Sbjct: 640 NQDDLEEEDVFLLDVWDQVFFWIGKHANEEEKKAAATTVQEYLKT 684
>sp|Q60604|ADSV_MOUSE Adseverin (Scinderin) (Gelsolin-like protein)
          Length = 715

 Score = 42.7 bits (99), Expect = 3e-04
 Identities = 24/66 (36%), Positives = 40/66 (60%), Gaps = 3/66 (4%)
 Frame = +2

Query: 20  ATGPLKFEKVAEGN-VSRSQINEDDVFIVDNGQV--IYVYVGENSSPNERKNALIYAHNY 190
           A+G +K   VAE N  S   +  ++ FI+D+G    I+V+ G+N++P ERK A+  A  +
Sbjct: 257 ASGSMKVTLVAEENPFSMGMLLSEECFILDHGAAKQIFVWKGKNANPQERKTAMKTAEEF 316

Query: 191 LKTTEY 208
           L+  +Y
Sbjct: 317 LQKMKY 322

 Score = 37.0 bits (84), Expect = 0.014
 Identities = 18/59 (30%), Positives = 37/59 (62%)
 Frame = +2

Query: 23  TGPLKFEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKT 199
           TG    E+V  G  ++  + EDDV ++D  + I++++G++++  E+K ++  A  YL+T
Sbjct: 623 TGRFIIEEVP-GEFTQDDLAEDDVMLLDAWEQIFIWIGKDANEVEKKESVKSAKMYLET 680
>sp|P40121|CAPG_HUMAN Macrophage capping protein (Actin-regulatory protein CAP-G)
          Length = 348

 Score = 42.0 bits (97), Expect = 4e-04
 Identities = 27/92 (29%), Positives = 47/92 (51%), Gaps = 3/92 (3%)
 Frame = +2

Query: 20  ATGPLKFEKVAEGN-VSRSQINEDDVFIVDNGQV--IYVYVGENSSPNERKNALIYAHNY 190
           ATG +   KVA+ +  +   +  DD F++DNG    IY++ G  ++  ER+ AL  A  +
Sbjct: 257 ATGQMNLTKVADSSPFALELLISDDCFVLDNGLCGKIYIWKGRKANEKERQAALQVAEGF 316

Query: 191 LKTTEYPQAPVTVINKNVKGKTAENFNNLVKE 286
           +   +Y  AP T +    +G+ +  F    K+
Sbjct: 317 ISRMQY--APNTQVEILPQGRESPIFKQFFKD 346
>sp|P14885|GELS_XENLA Gelsolin (Actin-depolymerizing factor) (ADF) (Brevin)
          Length = 417

 Score = 41.6 bits (96), Expect = 6e-04
 Identities = 19/59 (32%), Positives = 35/59 (59%)
 Frame = +2

Query: 23  TGPLKFEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKT 199
           TG    E+V  G +S+  +  DDV ++D    +YV+VG  +  +E+K A+  A+ Y+++
Sbjct: 311 TGRFIIEEVP-GEISQDDLATDDVMLLDTWDQVYVWVGNEAQEDEKKEAIASAYKYIES 368

 Score = 29.6 bits (65), Expect = 2.3
 Identities = 12/44 (27%), Positives = 24/44 (54%)
 Frame = +2

Query: 38  FEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNA 169
           F +  E + + S +N +D F++      Y++VG+ S+  E+  A
Sbjct: 211 FSRAVEVDNTASNLNSNDAFVLTTPSASYLWVGQGSTNVEKNGA 254
>sp|O81645|VILI3_ARATH Villin-3
          Length = 966

 Score = 40.4 bits (93), Expect = 0.001
 Identities = 21/44 (47%), Positives = 28/44 (63%)
 Frame = +2

Query: 65  SRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLK 196
           +RS +N DDVFI+D  + IY + G NS+  ER  AL+    YLK
Sbjct: 158 ARSSLNHDDVFILDTKEKIYQFNGANSNIQERAKALVVI-QYLK 200

 Score = 40.4 bits (93), Expect = 0.001
 Identities = 24/91 (26%), Positives = 47/91 (51%), Gaps = 7/91 (7%)
 Frame = +2

Query: 23  TGPLKFEKVAEGNV-------SRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYA 181
           T P K   +A+G V       S+S +  +  +++D G  I+++VG  +   ERK A+  A
Sbjct: 251 TTPPKLYSIADGQVESIDGDLSKSMLENNKCYLLDCGSEIFIWVGRVTQVEERKTAIQAA 310

Query: 182 HNYLKTTEYPQAPVTVINKNVKGKTAENFNN 274
            +++ +   P+A  T I + ++G    +F +
Sbjct: 311 EDFVASENRPKA--TRITRVIQGYEPHSFKS 339
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,919,729
Number of Sequences: 369166
Number of extensions: 688673
Number of successful extensions: 1767
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1699
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1766
length of database: 68,354,980
effective HSP length: 88
effective length of database: 52,098,300
effective search space used: 1719243900
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)