Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_A02
(367 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P10733|SEVE_DICDI Severin 65 4e-11
sp|Q24800|SEVE_ECHGR Severin 59 3e-09
sp|Q27319|GELS_HOMAM Gelsolin, cytoplasmic (Actin-depolymer... 50 2e-06
sp|Q07171|GELS_DROME Gelsolin precursor 49 5e-06
sp|P09327|VILI_HUMAN Villin-1 45 7e-05
sp|Q62468|VILI_MOUSE Villin-1 44 2e-04
sp|Q60604|ADSV_MOUSE Adseverin (Scinderin) (Gelsolin-like p... 43 3e-04
sp|P40121|CAPG_HUMAN Macrophage capping protein (Actin-regu... 42 4e-04
sp|P14885|GELS_XENLA Gelsolin (Actin-depolymerizing factor)... 42 6e-04
sp|O81645|VILI3_ARATH Villin-3 40 0.001
>sp|P10733|SEVE_DICDI Severin
Length = 362
Score = 65.5 bits (158), Expect = 4e-11
Identities = 32/87 (36%), Positives = 53/87 (60%)
Frame = +2
Query: 20 ATGPLKFEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKT 199
A+G LKF +V+ G +++S + +DVFI+D G IY ++G SSPNE+K A +A YL
Sbjct: 276 ASGSLKFSEVSRGKINKSSLKSEDVFIIDLGNEIYTWIGSKSSPNEKKTAFSHATQYLVN 335
Query: 200 TEYPQAPVTVINKNVKGKTAENFNNLV 280
+ + T I + ++ T ++F L+
Sbjct: 336 NK--RCEYTPIVRVLENGTNQSFETLL 360
Score = 38.9 bits (89), Expect = 0.004
Identities = 19/45 (42%), Positives = 26/45 (57%)
Frame = +2
Query: 35 KFEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNA 169
K KVAE ++ S +N D F++D G IY + G SSP E+ A
Sbjct: 170 KNAKVAEVPLATSSLNSGDCFLLDAGLTIYQFNGSKSSPQEKNKA 214
>sp|Q24800|SEVE_ECHGR Severin
Length = 374
Score = 59.3 bits (142), Expect = 3e-09
Identities = 27/71 (38%), Positives = 47/71 (66%)
Frame = +2
Query: 20 ATGPLKFEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKT 199
++G L+ V+EG+ S+ I DDV+I+ + ++VY+G++ S E++NAL AH +L+T
Sbjct: 289 SSGKLELTIVSEGSASKHDIKPDDVYIILTKEGLFVYIGKDCSVLEKRNALSNAHKFLQT 348
Query: 200 TEYPQAPVTVI 232
P P+TV+
Sbjct: 349 CPNPFLPITVV 359
>sp|Q27319|GELS_HOMAM Gelsolin, cytoplasmic (Actin-depolymerizing factor) (ADF)
Length = 754
Score = 49.7 bits (117), Expect = 2e-06
Identities = 24/72 (33%), Positives = 42/72 (58%), Gaps = 1/72 (1%)
Frame = +2
Query: 65 SRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKTTEYPQA-PVTVINKN 241
++ +NEDDV ++D+G +YV+VG+ S E++ A A NY+KT + TVI +
Sbjct: 652 AQEDLNEDDVMVLDSGDEVYVWVGQGSDDQEKEKAFTMAENYIKTDPTERTLDATVILRI 711
Query: 242 VKGKTAENFNNL 277
+G+ F ++
Sbjct: 712 NQGEEPAAFTSI 723
Score = 40.4 bits (93), Expect = 0.001
Identities = 17/62 (27%), Positives = 34/62 (54%)
Frame = +2
Query: 26 GPLKFEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKTTE 205
G ++ + E + +S ++ D F++D G +YV++G SS E+ ++ A Y++
Sbjct: 261 GVIQTNMIGEKPLLQSMLDSGDCFLLDTGVGVYVWIGSGSSKKEKVKSMELAAGYMEKKG 320
Query: 206 YP 211
YP
Sbjct: 321 YP 322
Score = 28.9 bits (63), Expect = 3.9
Identities = 14/51 (27%), Positives = 27/51 (52%)
Frame = +2
Query: 8 FGTRATGPLKFEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNER 160
F R T + + V+ S +N DDVF+++ Y+++G+ +S E+
Sbjct: 530 FRVRGTCDFDTRAIQQTEVAGS-LNSDDVFVLETPGKTYLWIGKGASEEEK 579
>sp|Q07171|GELS_DROME Gelsolin precursor
Length = 798
Score = 48.5 bits (114), Expect = 5e-06
Identities = 24/83 (28%), Positives = 51/83 (61%)
Frame = +2
Query: 20 ATGPLKFEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKT 199
A+G LK + + + ++++ ++ + FI+D G I+V+VG+ ++ E+ +A+ A +L+T
Sbjct: 318 ASGKLKVDIIGQKPLTQAMLDTRECFILDTGSGIFVWVGKGATQKEKTDAMAKAQEFLRT 377
Query: 200 TEYPQAPVTVINKNVKGKTAENF 268
+YP T I++ V+G + F
Sbjct: 378 KKYP--AWTQIHRIVEGSESAPF 398
Score = 38.1 bits (87), Expect = 0.006
Identities = 21/64 (32%), Positives = 34/64 (53%)
Frame = +2
Query: 44 KVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKTTEYPQAPV 223
+V + N+S S +N D FI+D G IYVYVG + E+ A+ A+ +A V
Sbjct: 204 RVRQVNLSVSSMNTGDCFILDAGSDIYVYVGSQAKRVEKLKAISAANQIRDQDHNGRARV 263
Query: 224 TVIN 235
+++
Sbjct: 264 QIVD 267
Score = 32.7 bits (73), Expect = 0.27
Identities = 19/55 (34%), Positives = 29/55 (52%)
Frame = +2
Query: 26 GPLKFEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNY 190
G LK E+VA+ + ++ DD+ ++D G IY++VG S E L A Y
Sbjct: 687 GFLKVEEVAQ--YEQEDLDSDDIMLLDAGDEIYLWVGYGVSEEENGKLLDTAKLY 739
>sp|P09327|VILI_HUMAN Villin-1
Length = 827
Score = 44.7 bits (104), Expect = 7e-05
Identities = 25/88 (28%), Positives = 49/88 (55%), Gaps = 1/88 (1%)
Frame = +2
Query: 26 GPLKFEKVAEGNVSRSQINEDDVFIVDNGQV-IYVYVGENSSPNERKNALIYAHNYLKTT 202
G L +VA +++ ++ +D +I+D G + IYV+ G+ ++ E+K A+ +A N++K
Sbjct: 263 GNLVVREVATRPLTQDLLSHEDCYILDQGGLKIYVWKGKKANEQEKKGAMSHALNFIKAK 322
Query: 203 EYPQAPVTVINKNVKGKTAENFNNLVKE 286
+YP P T + G + F L ++
Sbjct: 323 QYP--PSTQVEVQNDGAESAVFQQLFQK 348
Score = 40.8 bits (94), Expect = 0.001
Identities = 18/61 (29%), Positives = 36/61 (59%), Gaps = 3/61 (4%)
Frame = +2
Query: 65 SRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKT---TEYPQAPVTVIN 235
++ + EDDVF++D ++ ++G++++ E+K A A YLKT P+ P+ V+
Sbjct: 640 NQDDLEEDDVFLLDVWDQVFFWIGKHANEEEKKAAATTAQEYLKTHPSGRDPETPIIVVK 699
Query: 236 K 238
+
Sbjct: 700 Q 700
>sp|Q62468|VILI_MOUSE Villin-1
Length = 827
Score = 43.5 bits (101), Expect = 2e-04
Identities = 25/88 (28%), Positives = 47/88 (53%), Gaps = 1/88 (1%)
Frame = +2
Query: 26 GPLKFEKVAEGNVSRSQINEDDVFIVDNGQV-IYVYVGENSSPNERKNALIYAHNYLKTT 202
G L +VA +++ + +D +I+D G + I+V+ G+N++ ER A+ A N++K
Sbjct: 263 GKLVVREVATRPLTQDLLKHEDCYILDQGGLKIFVWKGKNANAQERSGAMSQALNFIKAK 322
Query: 203 EYPQAPVTVINKNVKGKTAENFNNLVKE 286
+YP P T + G + F L ++
Sbjct: 323 QYP--PSTQVEVQNDGAESPIFQQLFQK 348
Score = 35.0 bits (79), Expect = 0.054
Identities = 13/45 (28%), Positives = 28/45 (62%)
Frame = +2
Query: 65 SRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKT 199
++ + E+DVF++D ++ ++G++++ E+K A YLKT
Sbjct: 640 NQDDLEEEDVFLLDVWDQVFFWIGKHANEEEKKAAATTVQEYLKT 684
>sp|Q60604|ADSV_MOUSE Adseverin (Scinderin) (Gelsolin-like protein)
Length = 715
Score = 42.7 bits (99), Expect = 3e-04
Identities = 24/66 (36%), Positives = 40/66 (60%), Gaps = 3/66 (4%)
Frame = +2
Query: 20 ATGPLKFEKVAEGN-VSRSQINEDDVFIVDNGQV--IYVYVGENSSPNERKNALIYAHNY 190
A+G +K VAE N S + ++ FI+D+G I+V+ G+N++P ERK A+ A +
Sbjct: 257 ASGSMKVTLVAEENPFSMGMLLSEECFILDHGAAKQIFVWKGKNANPQERKTAMKTAEEF 316
Query: 191 LKTTEY 208
L+ +Y
Sbjct: 317 LQKMKY 322
Score = 37.0 bits (84), Expect = 0.014
Identities = 18/59 (30%), Positives = 37/59 (62%)
Frame = +2
Query: 23 TGPLKFEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKT 199
TG E+V G ++ + EDDV ++D + I++++G++++ E+K ++ A YL+T
Sbjct: 623 TGRFIIEEVP-GEFTQDDLAEDDVMLLDAWEQIFIWIGKDANEVEKKESVKSAKMYLET 680
>sp|P40121|CAPG_HUMAN Macrophage capping protein (Actin-regulatory protein CAP-G)
Length = 348
Score = 42.0 bits (97), Expect = 4e-04
Identities = 27/92 (29%), Positives = 47/92 (51%), Gaps = 3/92 (3%)
Frame = +2
Query: 20 ATGPLKFEKVAEGN-VSRSQINEDDVFIVDNGQV--IYVYVGENSSPNERKNALIYAHNY 190
ATG + KVA+ + + + DD F++DNG IY++ G ++ ER+ AL A +
Sbjct: 257 ATGQMNLTKVADSSPFALELLISDDCFVLDNGLCGKIYIWKGRKANEKERQAALQVAEGF 316
Query: 191 LKTTEYPQAPVTVINKNVKGKTAENFNNLVKE 286
+ +Y AP T + +G+ + F K+
Sbjct: 317 ISRMQY--APNTQVEILPQGRESPIFKQFFKD 346
>sp|P14885|GELS_XENLA Gelsolin (Actin-depolymerizing factor) (ADF) (Brevin)
Length = 417
Score = 41.6 bits (96), Expect = 6e-04
Identities = 19/59 (32%), Positives = 35/59 (59%)
Frame = +2
Query: 23 TGPLKFEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLKT 199
TG E+V G +S+ + DDV ++D +YV+VG + +E+K A+ A+ Y+++
Sbjct: 311 TGRFIIEEVP-GEISQDDLATDDVMLLDTWDQVYVWVGNEAQEDEKKEAIASAYKYIES 368
Score = 29.6 bits (65), Expect = 2.3
Identities = 12/44 (27%), Positives = 24/44 (54%)
Frame = +2
Query: 38 FEKVAEGNVSRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNA 169
F + E + + S +N +D F++ Y++VG+ S+ E+ A
Sbjct: 211 FSRAVEVDNTASNLNSNDAFVLTTPSASYLWVGQGSTNVEKNGA 254
>sp|O81645|VILI3_ARATH Villin-3
Length = 966
Score = 40.4 bits (93), Expect = 0.001
Identities = 21/44 (47%), Positives = 28/44 (63%)
Frame = +2
Query: 65 SRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYAHNYLK 196
+RS +N DDVFI+D + IY + G NS+ ER AL+ YLK
Sbjct: 158 ARSSLNHDDVFILDTKEKIYQFNGANSNIQERAKALVVI-QYLK 200
Score = 40.4 bits (93), Expect = 0.001
Identities = 24/91 (26%), Positives = 47/91 (51%), Gaps = 7/91 (7%)
Frame = +2
Query: 23 TGPLKFEKVAEGNV-------SRSQINEDDVFIVDNGQVIYVYVGENSSPNERKNALIYA 181
T P K +A+G V S+S + + +++D G I+++VG + ERK A+ A
Sbjct: 251 TTPPKLYSIADGQVESIDGDLSKSMLENNKCYLLDCGSEIFIWVGRVTQVEERKTAIQAA 310
Query: 182 HNYLKTTEYPQAPVTVINKNVKGKTAENFNN 274
+++ + P+A T I + ++G +F +
Sbjct: 311 EDFVASENRPKA--TRITRVIQGYEPHSFKS 339
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,919,729
Number of Sequences: 369166
Number of extensions: 688673
Number of successful extensions: 1767
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1699
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1766
length of database: 68,354,980
effective HSP length: 88
effective length of database: 52,098,300
effective search space used: 1719243900
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)