Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= DrC_01486 (992 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|Q8UVX0|PIWI_BRARE Piwi protein 273 7e-73 sp|Q9VKM1|PIWI_DROME Piwi protein 179 8e-45 sp|O77503|I2C2_RABIT Eukaryotic translation initiation fact... 135 2e-31 sp|Q9UKV8|I2C2_HUMAN Eukaryotic translation initiation fact... 135 2e-31 sp|Q8CJG0|I2C2_MOUSE Eukaryotic translation initiation fact... 134 3e-31 sp|Q9H9G7|I2C3_HUMAN Eukaryotic translation initiation fact... 134 5e-31 sp|Q8CJF9|I2C3_MOUSE Eukaryotic translation initiation fact... 134 5e-31 sp|Q9QZ81|I2C2_RAT Eukaryotic translation initiation factor... 133 7e-31 sp|Q8CJF8|I2C4_MOUSE Eukaryotic translation initiation fact... 132 1e-30 sp|Q9HCK5|I2C4_HUMAN Eukaryotic translation initiation fact... 130 4e-30
>sp|Q8UVX0|PIWI_BRARE Piwi protein Length = 858 Score = 273 bits (697), Expect = 7e-73 Identities = 132/286 (46%), Positives = 183/286 (63%) Frame = +3 Query: 3 ILSAVREDRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWA 182 IL R+D+Y+ +KK+ VDCP PSQ V+++T+ + L +VA IALQ+N K+GGELW+ Sbjct: 556 ILPTNRKDKYDCVKKYLCVDCPTPSQCVVSRTISKPQALMTVATKIALQMNCKMGGELWS 615 Query: 183 VKIPIKKFMVVGLDVWHDAKXXXXXXXXXXXXTNALCTRWFSKSHLQEHDKEIVYVLQSC 362 V+IP+++ M+VG+D +HD N +RWFSK LQ +EI+ L+ Sbjct: 616 VEIPLRQLMIVGIDCYHDTAAGKRSIGAMVASLNQGMSRWFSKCVLQNRGQEIIDALKGS 675 Query: 363 MLSLLKAYFEENNFLPDTIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYN 542 + LKAY + NN LP I +YRDGV DG L V E+ Q + ++ DY+P + Sbjct: 676 LQGALKAYLKYNNSLPSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQDYEPKLSVV 735 Query: 543 VVQKRINTRLYVSDPKNKGQVNNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYV 722 VV+KRI++R + + G++ NP PGT++D VTR YDFF+VSQ+VR G V PTHY Sbjct: 736 VVKKRISSRFFA---RIDGKIANPPPGTVIDTEVTRPEWYDFFIVSQAVRFGCVAPTHYN 792 Query: 723 VLCDNSRYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYM 860 V+ DNS P +Q + YK CH+YYNW G VRVPAPC YAHKLA++ Sbjct: 793 VVFDNSGLKPDHMQRLTYKLCHMYYNWQGIVRVPAPCQYAHKLAFL 838
>sp|Q9VKM1|PIWI_DROME Piwi protein Length = 843 Score = 179 bits (455), Expect = 8e-45 Identities = 110/297 (37%), Positives = 156/297 (52%), Gaps = 7/297 (2%) Frame = +3 Query: 24 DRYNAIKKFCYVDCPVPSQVVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKK 203 +RY++IKK YVD VP+QVV +T K L S+A IA+Q+N KLG W +++P+ Sbjct: 549 ERYSSIKKRGYVDRAVPTQVVTLKTTKNR-SLMSIATKIAIQLNCKLGYTPWMIELPLSG 607 Query: 204 FMVVGLDVWHDAKXXXXXXXXXXXXTNALC-TRWFSKSHLQEHDKEIVYVLQSCMLSLLK 380 M +G D+ + + + +FS + L + L+ Sbjct: 608 LMTIGFDIAKSTRDRKRAYGALIASMDLQQNSTYFSTVTECSAFDVLANTLWPMIAKALR 667 Query: 381 AYFEENNFLPDTIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYK------PNMVYN 542 Y E+ LP I YRDGVS G L + + E++ + E +Y P + Y Sbjct: 668 QYQHEHRKLPSRIVFYRDGVSSGSLKQLFEFEVKD---IIEKLKTEYARVQLSPPQLAYI 724 Query: 543 VVQKRINTRLYVSDPKNKGQVNNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYV 722 VV + +NTR +++ GQ NP PGTIVD +T YDF+LVSQ VRQGTV+PT Y Sbjct: 725 VVTRSMNTRFFLN-----GQ--NPPPGTIVDDVITLPERYDFYLVSQQVRQGTVSPTSYN 777 Query: 723 VLCDNSRYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAYMGWSKFKSRAQS 893 VL + +P ++Q + YK CH+YYNW GT RVPA C YA KLA + + S Q+ Sbjct: 778 VLYSSMGLSPEKMQKLTYKMCHLYYNWSGTTRVPAVCQYAKKLATLVGTNLHSIPQN 834
>sp|O77503|I2C2_RABIT Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2) (Argonaute-2) Length = 840 Score = 135 bits (339), Expect = 2e-31 Identities = 83/250 (33%), Positives = 130/250 (52%), Gaps = 9/250 (3%) Frame = +3 Query: 135 NIALQINAKLGGE----LWAVKIPIKKFMVV--GLDVWHD--AKXXXXXXXXXXXXTNAL 290 N+ L+IN KLGG L + P+ + V+ G DV H +A Sbjct: 543 NLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAH 602 Query: 291 CTRWFSKSHLQEHDKEIVYVLQSCMLSLLKAYFEENNFLPDTIFMYRDGVSDGQLGYVQK 470 R+ + +Q+H +EI+ L + + LL +++ F P I YRDGVS+GQ V Sbjct: 603 PNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLH 662 Query: 471 TEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNK-GQVNNPNPGTIVDHTVT 647 E+ + DY+P + + VVQKR +TRL+ +D + G+ N GT VD +T Sbjct: 663 HELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKIT 722 Query: 648 RANLYDFFLVSQSVRQGTVTPTHYVVLCDNSRYTPHQVQLMAYKTCHIYYNWPGTVRVPA 827 +DF+L S + QGT P+HY VL D++R++ ++Q++ Y+ CH Y +V +PA Sbjct: 723 HPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPA 782 Query: 828 PCMYAHKLAY 857 P YAH +A+ Sbjct: 783 PAYYAHLVAF 792
>sp|Q9UKV8|I2C2_HUMAN Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2) (Argonaute-2) (Slicer protein) (PAZ Piwi domain protein) (PPD) Length = 851 Score = 135 bits (339), Expect = 2e-31 Identities = 83/250 (33%), Positives = 130/250 (52%), Gaps = 9/250 (3%) Frame = +3 Query: 135 NIALQINAKLGGE----LWAVKIPIKKFMVV--GLDVWHD--AKXXXXXXXXXXXXTNAL 290 N+ L+IN KLGG L + P+ + V+ G DV H +A Sbjct: 554 NLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAH 613 Query: 291 CTRWFSKSHLQEHDKEIVYVLQSCMLSLLKAYFEENNFLPDTIFMYRDGVSDGQLGYVQK 470 R+ + +Q+H +EI+ L + + LL +++ F P I YRDGVS+GQ V Sbjct: 614 PNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLH 673 Query: 471 TEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNK-GQVNNPNPGTIVDHTVT 647 E+ + DY+P + + VVQKR +TRL+ +D + G+ N GT VD +T Sbjct: 674 HELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKIT 733 Query: 648 RANLYDFFLVSQSVRQGTVTPTHYVVLCDNSRYTPHQVQLMAYKTCHIYYNWPGTVRVPA 827 +DF+L S + QGT P+HY VL D++R++ ++Q++ Y+ CH Y +V +PA Sbjct: 734 HPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPA 793 Query: 828 PCMYAHKLAY 857 P YAH +A+ Sbjct: 794 PAYYAHLVAF 803
>sp|Q8CJG0|I2C2_MOUSE Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2) (Argonaute-2) (Slicer protein) (Piwi/argonaute family protein meIF2C2) Length = 860 Score = 134 bits (338), Expect = 3e-31 Identities = 83/250 (33%), Positives = 130/250 (52%), Gaps = 9/250 (3%) Frame = +3 Query: 135 NIALQINAKLGGE----LWAVKIPIKKFMVV--GLDVWHD--AKXXXXXXXXXXXXTNAL 290 N+ L+IN KLGG L + P+ + V+ G DV H +A Sbjct: 563 NLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAH 622 Query: 291 CTRWFSKSHLQEHDKEIVYVLQSCMLSLLKAYFEENNFLPDTIFMYRDGVSDGQLGYVQK 470 R+ + +Q+H +EI+ L + + LL +++ F P I YRDGVS+GQ V Sbjct: 623 PNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLH 682 Query: 471 TEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNK-GQVNNPNPGTIVDHTVT 647 E+ + DY+P + + VVQKR +TRL+ +D + G+ N GT VD +T Sbjct: 683 HELLAIREACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKIT 742 Query: 648 RANLYDFFLVSQSVRQGTVTPTHYVVLCDNSRYTPHQVQLMAYKTCHIYYNWPGTVRVPA 827 +DF+L S + QGT P+HY VL D++R++ ++Q++ Y+ CH Y +V +PA Sbjct: 743 HPTEFDFYLCSHAGIQGTGRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPA 802 Query: 828 PCMYAHKLAY 857 P YAH +A+ Sbjct: 803 PAYYAHLVAF 812
>sp|Q9H9G7|I2C3_HUMAN Eukaryotic translation initiation factor 2C 3 (eIF2C 3) (eIF-2C 3) (Argonaute-3) Length = 860 Score = 134 bits (336), Expect = 5e-31 Identities = 89/269 (33%), Positives = 137/269 (50%), Gaps = 10/269 (3%) Frame = +3 Query: 81 VVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMV-------VGLDVWHD- 236 V + +K P+ S N+ L+IN KLGG + + +P ++ V +G DV H Sbjct: 548 VQVKNVIKTSPQTLS---NLCLKINVKLGG-INNILVPHQRPSVFQQPVIFLGADVTHPP 603 Query: 237 -AKXXXXXXXXXXXXTNALCTRWFSKSHLQEHDKEIVYVLQSCMLSLLKAYFEENNFLPD 413 +A +R+ + +Q +EI+ L S + LL +++ F P Sbjct: 604 AGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFKPT 663 Query: 414 TIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKN 593 I YRDGVS+GQ V E+ + S DY+P + Y VVQKR +TRL+ +D Sbjct: 664 RIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTE 723 Query: 594 K-GQVNNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSRYTPHQVQLM 770 + G+ N GT VD +T +DF+L S + QGT P+HY VL D++ +T ++QL+ Sbjct: 724 RVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFTADELQLL 783 Query: 771 AYKTCHIYYNWPGTVRVPAPCMYAHKLAY 857 Y+ CH Y +V +PAP YAH +A+ Sbjct: 784 TYQLCHTYVRCTRSVSIPAPAYYAHLVAF 812
>sp|Q8CJF9|I2C3_MOUSE Eukaryotic translation initiation factor 2C 3 (eIF2C 3) (eIF-2C 3) (Argonaute-3) (Piwi/argonaute family protein meIF2C3) Length = 860 Score = 134 bits (336), Expect = 5e-31 Identities = 89/269 (33%), Positives = 137/269 (50%), Gaps = 10/269 (3%) Frame = +3 Query: 81 VVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMV-------VGLDVWHD- 236 V + +K P+ S N+ L+IN KLGG + + +P ++ V +G DV H Sbjct: 548 VQVKNVIKTSPQTLS---NLCLKINVKLGG-INNILVPHQRPSVFQQPVIFLGADVTHPP 603 Query: 237 -AKXXXXXXXXXXXXTNALCTRWFSKSHLQEHDKEIVYVLQSCMLSLLKAYFEENNFLPD 413 +A +R+ + +Q +EI+ L S + LL +++ F P Sbjct: 604 AGDGKKPSIAAVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFKPT 663 Query: 414 TIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKN 593 I YRDGVS+GQ V E+ + S DY+P + Y VVQKR +TRL+ +D Sbjct: 664 RIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQPGITYIVVQKRHHTRLFCADRTE 723 Query: 594 K-GQVNNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNSRYTPHQVQLM 770 + G+ N GT VD +T +DF+L S + QGT P+HY VL D++ +T ++QL+ Sbjct: 724 RVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNFFTADELQLL 783 Query: 771 AYKTCHIYYNWPGTVRVPAPCMYAHKLAY 857 Y+ CH Y +V +PAP YAH +A+ Sbjct: 784 TYQLCHTYVRCTRSVSIPAPAYYAHLVAF 812
>sp|Q9QZ81|I2C2_RAT Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2) (Argonaute-2) (Golgi ER protein 95 kDa) (GERp95) Length = 860 Score = 133 bits (335), Expect = 7e-31 Identities = 82/250 (32%), Positives = 130/250 (52%), Gaps = 9/250 (3%) Frame = +3 Query: 135 NIALQINAKLGGE----LWAVKIPIKKFMVV--GLDVWHD--AKXXXXXXXXXXXXTNAL 290 N+ L+IN KLGG L + P+ + V+ G DV H +A Sbjct: 563 NLCLKINVKLGGVNNILLPQGRPPVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAH 622 Query: 291 CTRWFSKSHLQEHDKEIVYVLQSCMLSLLKAYFEENNFLPDTIFMYRDGVSDGQLGYVQK 470 R+ + +Q+H +EI+ L + + LL +++ F P I YRDGVS+GQ V Sbjct: 623 PNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFQQVLH 682 Query: 471 TEIEQFFKVFESFSADYKPNMVYNVVQKRINTRLYVSDPKNK-GQVNNPNPGTIVDHTVT 647 E+ + +Y+P + + VVQKR +TRL+ +D + G+ N GT VD +T Sbjct: 683 HELLAIREACIKLEKEYQPGITFIVVQKRHHTRLFCTDKNERVGKSGNIPAGTTVDTKIT 742 Query: 648 RANLYDFFLVSQSVRQGTVTPTHYVVLCDNSRYTPHQVQLMAYKTCHIYYNWPGTVRVPA 827 +DF+L S + QGT P+HY VL D++R++ ++Q++ Y+ CH Y +V +PA Sbjct: 743 HPTEFDFYLCSHAGIQGTSRPSHYHVLWDDNRFSSDELQILTYQLCHTYVRCTRSVSIPA 802 Query: 828 PCMYAHKLAY 857 P YAH +A+ Sbjct: 803 PAYYAHLVAF 812
>sp|Q8CJF8|I2C4_MOUSE Eukaryotic translation initiation factor 2C 4 (eIF2C 4) (eIF-2C 4) (Argonaute-4) (Piwi/argonaute family protein meIF2C4) Length = 861 Score = 132 bits (333), Expect = 1e-30 Identities = 95/279 (34%), Positives = 139/279 (49%), Gaps = 20/279 (7%) Frame = +3 Query: 81 VVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMV-------VGLDVWH-- 233 V + +K P+ S N+ L++NAKLGG + V +P ++ V +G DV H Sbjct: 539 VQIKNVVKTSPQTLS---NLCLKMNAKLGG-INNVPVPHQRPSVFQQPVIFLGADVTHPP 594 Query: 234 --DAKXXXXXXXXXXXX---TNALCTRWFSKSH---LQE--HDKEIVYVLQSCMLSLLKA 383 D K + T W S QE + +E+V L S LL Sbjct: 595 AGDGKKPSIAAVVGSMDGHPSRYCATVWVQTSRQEIAQELLYSQEVVQDLTSMARELLIQ 654 Query: 384 YFEENNFLPDTIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRIN 563 +++ F P I YR GVS+GQ+ V E+ K S DY+P + Y VVQKR + Sbjct: 655 FYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHH 714 Query: 564 TRLYVSDPKNK-GQVNNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNS 740 TRL+ +D + G+ N GT VD TVT + +DF+L S + QGT P+HY VL D++ Sbjct: 715 TRLFCADKMERVGKSGNVPAGTTVDSTVTHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDN 774 Query: 741 RYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAY 857 +T ++QL+ Y+ CH Y +V +PAP YA +A+ Sbjct: 775 CFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAF 813
>sp|Q9HCK5|I2C4_HUMAN Eukaryotic translation initiation factor 2C 4 (eIF2C 4) (eIF-2C 4) (Argonaute-4) Length = 861 Score = 130 bits (328), Expect = 4e-30 Identities = 92/279 (32%), Positives = 140/279 (50%), Gaps = 20/279 (7%) Frame = +3 Query: 81 VVLAQTLKEGPKLNSVAVNIALQINAKLGGELWAVKIPIKKFMV-------VGLDVWH-- 233 V + +K P+ S N+ L+INAKLGG + V +P ++ V +G DV H Sbjct: 539 VQVKNVVKTSPQTLS---NLCLKINAKLGG-INNVLVPHQRPSVFQQPVIFLGADVTHPP 594 Query: 234 --DAKXXXXXXXXXXXX--TNALCTRWFSKSHLQEHDKEIVYV------LQSCMLSLLKA 383 D K + C ++ QE +E++Y L + + LL Sbjct: 595 AGDGKKPSIAAVVGSMDGHPSRYCATVRVQTSRQEISQELLYSQEVIQDLTNMVRELLIQ 654 Query: 384 YFEENNFLPDTIFMYRDGVSDGQLGYVQKTEIEQFFKVFESFSADYKPNMVYNVVQKRIN 563 +++ F P I YR GVS+GQ+ V E+ K S DY+P + Y VVQKR + Sbjct: 655 FYKSTRFKPTRIIYYRGGVSEGQMKQVAWPELIAIRKACISLEEDYRPGITYIVVQKRHH 714 Query: 564 TRLYVSDPKNK-GQVNNPNPGTIVDHTVTRANLYDFFLVSQSVRQGTVTPTHYVVLCDNS 740 TRL+ +D + G+ N GT VD T+T + +DF+L S + QGT P+HY VL D++ Sbjct: 715 TRLFCADKTERVGKSGNVPAGTTVDSTITHPSEFDFYLCSHAGIQGTSRPSHYQVLWDDN 774 Query: 741 RYTPHQVQLMAYKTCHIYYNWPGTVRVPAPCMYAHKLAY 857 +T ++QL+ Y+ CH Y +V +PAP YA +A+ Sbjct: 775 CFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYARLVAF 813
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 110,243,610 Number of Sequences: 369166 Number of extensions: 2211882 Number of successful extensions: 5186 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 5016 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 5164 length of database: 68,354,980 effective HSP length: 111 effective length of database: 47,849,395 effective search space used: 10479017505 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)