Planaria EST Database


DrC_01391

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01391
         (880 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8BXQ2|PIGT_MOUSE  GPI transamidase component PIG-T precu...   146   8e-35
sp|Q969N2|PIGT_HUMAN  GPI transamidase component PIG-T precu...   141   2e-33
sp|P38875|GP16_YEAST  GPI transamidase component GPI16 precu...    80   7e-15
sp|O94380|GPI16_SCHPO  GPI transamidase component PIG-T homo...    66   1e-10
sp|Q03058|CAT_STRAG  Chloramphenicol acetyltransferase (CAT)       32   2.3  
sp|P36882|CAT4_STAAU  Chloramphenicol acetyltransferase (CAT)      32   3.0  
sp|P58742|AAAS_MOUSE  Aladin (Adracalin)                           31   5.2  
sp|P06135|CAT3_STAAU  Chloramphenicol acetyltransferase (CAT)      31   5.2  
sp|P00486|CAT2_STAAU  Chloramphenicol acetyltransferase (CAT)      31   5.2  
sp|Q8XUZ8|RPOB_RALSO  DNA-directed RNA polymerase beta chain...    31   5.2  
>sp|Q8BXQ2|PIGT_MOUSE GPI transamidase component PIG-T precursor
           (Phosphatidylinositol-glycan biosynthesis, class T
           protein) (Neuronal development-associated protein 7)
          Length = 582

 Score =  146 bits (368), Expect = 8e-35
 Identities = 102/302 (33%), Positives = 153/302 (50%), Gaps = 18/302 (5%)
 Frame = +2

Query: 8   WGISPNSLPSESAELWAWFEPFTKEIE--WNHLSHALSGLFCTSLNKLSNHKEVISPKWS 181
           WG      PS  AELW WF+    +++  W  LS+ LSG+FC SLN + +    ++P  S
Sbjct: 103 WGPPFLQAPS-GAELWVWFQDTVTDVDKSWRELSNVLSGIFCASLNFI-DATNTVTPTAS 160

Query: 182 FSPEGLTHKFRNDSLIRKAVLSGEAICTENLTPWIKLLPCKKLAGLAQLLKPSRLFSSHF 361
           F P GL +   +D  +R AVL  E +CTENLTPW KLLPC   AGL+ LLK  RLF + +
Sbjct: 161 FKPLGLAND-TDDYFLRYAVLpreVVCTENLTPWKKLLPCSSKAGLSVLLKADRLFHTSY 219

Query: 362 TSITMNVKKICLTNDCTEIGRLLHQTITLVLDRKLFGNYYNEWDLIELLNTKSEIEVCPL 541
            S  ++++ IC    CT I   L QT+++V D  + G    +W L  +  +++  E CPL
Sbjct: 220 HSQAVHIRPICRNAHCTSISWELRQTLSVVFDAFITGQGKKDWSLFRMF-SRTLTEACPL 278

Query: 542 AEISRIYVHGFG---PVDTSKIKP------TGVIQSNQLVY-----YDLATFNKTFNKIN 679
           A  S +YV   G     +T ++ P        VI   +  Y     +D A  N + N +N
Sbjct: 279 ASQSLVYVDITGYSQDNETLEVSPPPTSTYQDVILGTRKTYAVYDLFDTAMINNSRN-LN 337

Query: 680 VKLSGVWPVDDINHLSRNCPF-QFTKFATGIGKVQGGIKATI-KSQSKNSIRVDYLESMP 853
           ++L    P D   + +   PF    ++ +G G  +G +   +  S    +  V  L+ +P
Sbjct: 338 IQLKWKRPPD---NEALPVPFLHAQRYVSGYGLQKGELSTLLYNSHPYRAFPVLLLDVVP 394

Query: 854 WY 859
           WY
Sbjct: 395 WY 396
>sp|Q969N2|PIGT_HUMAN GPI transamidase component PIG-T precursor
           (Phosphatidylinositol-glycan biosynthesis, class T
           protein)
          Length = 578

 Score =  141 bits (356), Expect = 2e-33
 Identities = 93/298 (31%), Positives = 141/298 (47%), Gaps = 14/298 (4%)
 Frame = +2

Query: 8   WGISPNSLPSESAELWAWFEPFTKEIE--WNHLSHALSGLFCTSLNKLSNHKEVISPKWS 181
           WG      PS  AELW WF+    +++  W  LS+ LSG+FC SLN + +    ++P  S
Sbjct: 99  WGPPFLQAPS-GAELWVWFQDTVTDVDKSWKELSNVLSGIFCASLNFIDS-TNTVTPTAS 156

Query: 182 FSPEGLTHKFRNDSLIRKAVLSGEAICTENLTPWIKLLPCKKLAGLAQLLKPSRLFSSHF 361
           F P GL +   +   +R AVL  E +CTENLTPW KLLPC   AGL+ LLK  RLF + +
Sbjct: 157 FKPLGLAND-TDHYFLRYAVLpreVVCTENLTPWKKLLPCSSKAGLSVLLKADRLFHTSY 215

Query: 362 TSITMNVKKICLTNDCTEIGRLLHQTITLVLDRKLFGNYYNEWDLIELLNTKSEIEVCPL 541
            S  ++++ +C    CT I   L QT+++V D  + G    +W L  +  +++  E CPL
Sbjct: 216 HSQAVHIRPVCRNARCTSISWELRQTLSVVFDAFITGQGKKDWSLFRMF-SRTLTEPCPL 274

Query: 542 AEISRIYVH-----------GFGPVDTSKIKPTGVIQSNQLVYYDLATFNKTFNKINVKL 688
           A  SR+YV               P  T+  +   +        YDL       N  N+ +
Sbjct: 275 ASESRVYVDITTYNQDNETLEVHPPPTTTYQDVILGTRKTYAIYDLLDTAMINNSRNLNI 334

Query: 689 SGVWPVDDINHLSRNCPFQFTKFATGIGKVQGGIKATI-KSQSKNSIRVDYLESMPWY 859
              W     N           ++ +G G  +G +   +  +    +  V  L+++PWY
Sbjct: 335 QLKWKRPPENEAPPVPFLHAQRYVSGYGLQKGELSTLLYNTHPYRAFPVLLLDTVPWY 392
>sp|P38875|GP16_YEAST GPI transamidase component GPI16 precursor
          Length = 602

 Score = 80.1 bits (196), Expect = 7e-15
 Identities = 50/137 (36%), Positives = 76/137 (55%), Gaps = 8/137 (5%)
 Frame = +2

Query: 8   WGISPNSLPSESA---ELWAWFEPFTKEI---EWNHLSHALSGLFCTSLNKLSNHKEVIS 169
           WG  P++     A   ELW+  +   +E     W  LS++LSGLFC+SLN +   +    
Sbjct: 103 WGQLPHAGKEAGASGVELWSQVQAMDQEQAFHNWKKLSNSLSGLFCSSLNFIDESRTTF- 161

Query: 170 PKWSFSPEGLTHKFRNDS--LIRKAVLSGEAICTENLTPWIKLLPCKKLAGLAQLLKPSR 343
           P+ S++ +     F +     + +A L  E ICTENLTP+IKLLP +  +GL  LL   +
Sbjct: 162 PRRSYASDIGAPLFNSTEKLYLMRASLPNEPICTENLTPFIKLLPTRGKSGLTSLLDGHK 221

Query: 344 LFSSHFTSITMNVKKIC 394
           LF S + SI++++  IC
Sbjct: 222 LFDSLWNSISLDIATIC 238

 Score = 33.1 bits (74), Expect = 1.0
 Identities = 11/41 (26%), Positives = 24/41 (58%)
 Frame = +2

Query: 737 PFQFTKFATGIGKVQGGIKATIKSQSKNSIRVDYLESMPWY 859
           P   ++  TG G+ +GG++    + +   +++ Y ES+PW+
Sbjct: 386 PIHVSRSLTGNGQDRGGMRIVFHNDNDTPVKLIYFESLPWF 426
>sp|O94380|GPI16_SCHPO GPI transamidase component PIG-T homolog precursor
          Length = 545

 Score = 65.9 bits (159), Expect = 1e-10
 Identities = 46/133 (34%), Positives = 66/133 (49%), Gaps = 6/133 (4%)
 Frame = +2

Query: 2   EKWGISPNS---LPSESAELWAWFEPFTKEIEWNHLSHALSGLFCTSLNKLSN---HKEV 163
           E W  SP++         E+WA+         W  L++ LSGL C SLN + +   ++  
Sbjct: 98  ENWKESPDNGFYSGGLGFEVWAFMANDPSMKYWLKLTNQLSGLLCASLNYIDSSNTYQPQ 157

Query: 164 ISPKWSFSPEGLTHKFRNDSLIRKAVLSGEAICTENLTPWIKLLPCKKLAGLAQLLKPSR 343
           +S   SFS    T  F        A L  E +CTENL+P  KLLPCK+ AG+A LL    
Sbjct: 158 LSYPGSFSFSNNTQYF--------ASLPQEDVCTENLSPLFKLLPCKRKAGIASLLDSHL 209

Query: 344 LFSSHFTSITMNV 382
            F + + S +++V
Sbjct: 210 FFDTDWHSFSIDV 222
>sp|Q03058|CAT_STRAG Chloramphenicol acetyltransferase (CAT)
          Length = 215

 Score = 32.0 bits (71), Expect = 2.3
 Identities = 17/50 (34%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
 Frame = +2

Query: 581 VDTSKIKPTGVIQSNQLVYYD-LATFNKTFNKINVKLSGVWPVDDINHLS 727
           V+ +K+  TG+   N+L Y+D L      FNK   K + +W   D N +S
Sbjct: 61  VNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWTESDKNFIS 110
>sp|P36882|CAT4_STAAU Chloramphenicol acetyltransferase (CAT)
          Length = 215

 Score = 31.6 bits (70), Expect = 3.0
 Identities = 27/102 (26%), Positives = 45/102 (44%), Gaps = 11/102 (10%)
 Frame = +2

Query: 455 DRK-LFGNYYNEWDLIELLNTKSEIEVCPLAEISRIYVHGFGP---------VDTSKIKP 604
           DRK  F +Y+N+     +     EI++    ++S+   +   P         V+ +K+  
Sbjct: 12  DRKEYFEHYFNQQTTYSITK---EIDITLFKDMSKKKGYEIYPSLIYAIMEVVNKNKVFR 68

Query: 605 TGVIQSNQLVYYD-LATFNKTFNKINVKLSGVWPVDDINHLS 727
           TG+   N+L Y+D L      FNK   K + +W   D N  S
Sbjct: 69  TGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWTESDNNFTS 110
>sp|P58742|AAAS_MOUSE Aladin (Adracalin)
          Length = 546

 Score = 30.8 bits (68), Expect = 5.2
 Identities = 20/64 (31%), Positives = 30/64 (46%), Gaps = 1/64 (1%)
 Frame = +2

Query: 593 KIKPTGVIQSNQLVYYDLATFNKTFNKINVKLSGVWPVDDINHLSRN-CPFQFTKFATGI 769
           ++ P G+IQ        L TF+ +FNK    LS  W    I H+       QF +F+  +
Sbjct: 440 ELLPCGIIQGEPGAQAQLITFHPSFNK-GALLSVCWSTGRITHIPLYFVNAQFPRFSPVL 498

Query: 770 GKVQ 781
           G+ Q
Sbjct: 499 GRAQ 502
>sp|P06135|CAT3_STAAU Chloramphenicol acetyltransferase (CAT)
          Length = 215

 Score = 30.8 bits (68), Expect = 5.2
 Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
 Frame = +2

Query: 581 VDTSKIKPTGVIQSNQLVYYD-LATFNKTFNKINVKLSGVWPVDDINHLS 727
           V+ +K+  TG+   N+L Y+D L      FNK   K + +W   D N  S
Sbjct: 61  VNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWTESDNNFTS 110
>sp|P00486|CAT2_STAAU Chloramphenicol acetyltransferase (CAT)
          Length = 215

 Score = 30.8 bits (68), Expect = 5.2
 Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
 Frame = +2

Query: 581 VDTSKIKPTGVIQSNQLVYYD-LATFNKTFNKINVKLSGVWPVDDINHLS 727
           V+ +K+  TG+   N+L Y+D L      FNK   K + +W   D N  S
Sbjct: 61  VNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWTESDNNFTS 110
>sp|Q8XUZ8|RPOB_RALSO DNA-directed RNA polymerase beta chain (RNAP beta subunit)
            (Transcriptase beta chain) (RNA polymerase beta subunit)
          Length = 1368

 Score = 30.8 bits (68), Expect = 5.2
 Identities = 18/44 (40%), Positives = 23/44 (52%)
 Frame = +2

Query: 416  IGRLLHQTITLVLDRKLFGNYYNEWDLIELLNTKSEIEVCPLAE 547
            IG +L         RKL G  YNE   +E L++ S+ EV  LAE
Sbjct: 1147 IGNMLKAQAKAAEMRKLLGQIYNESGKVEDLDSLSDAEVLELAE 1190
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 94,661,761
Number of Sequences: 369166
Number of extensions: 1783377
Number of successful extensions: 4354
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4234
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4344
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8742211660
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)