Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_004_E21
(880 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q8BXQ2|PIGT_MOUSE GPI transamidase component PIG-T precu... 146 8e-35
sp|Q969N2|PIGT_HUMAN GPI transamidase component PIG-T precu... 141 2e-33
sp|P38875|GP16_YEAST GPI transamidase component GPI16 precu... 80 7e-15
sp|O94380|GPI16_SCHPO GPI transamidase component PIG-T homo... 66 1e-10
sp|Q03058|CAT_STRAG Chloramphenicol acetyltransferase (CAT) 32 2.3
sp|P36882|CAT4_STAAU Chloramphenicol acetyltransferase (CAT) 32 3.0
sp|P58742|AAAS_MOUSE Aladin (Adracalin) 31 5.2
sp|P06135|CAT3_STAAU Chloramphenicol acetyltransferase (CAT) 31 5.2
sp|P00486|CAT2_STAAU Chloramphenicol acetyltransferase (CAT) 31 5.2
sp|Q8XUZ8|RPOB_RALSO DNA-directed RNA polymerase beta chain... 31 5.2
>sp|Q8BXQ2|PIGT_MOUSE GPI transamidase component PIG-T precursor
(Phosphatidylinositol-glycan biosynthesis, class T
protein) (Neuronal development-associated protein 7)
Length = 582
Score = 146 bits (368), Expect = 8e-35
Identities = 102/302 (33%), Positives = 153/302 (50%), Gaps = 18/302 (5%)
Frame = +2
Query: 8 WGISPNSLPSESAELWAWFEPFTKEIE--WNHLSHALSGLFCTSLNKLSNHKEVISPKWS 181
WG PS AELW WF+ +++ W LS+ LSG+FC SLN + + ++P S
Sbjct: 103 WGPPFLQAPS-GAELWVWFQDTVTDVDKSWRELSNVLSGIFCASLNFI-DATNTVTPTAS 160
Query: 182 FSPEGLTHKFRNDSLIRKAVLSGEAICTENLTPWIKLLPCKKLAGLAQLLKPSRLFSSHF 361
F P GL + +D +R AVL E +CTENLTPW KLLPC AGL+ LLK RLF + +
Sbjct: 161 FKPLGLAND-TDDYFLRYAVLpreVVCTENLTPWKKLLPCSSKAGLSVLLKADRLFHTSY 219
Query: 362 TSITMNVKKICLTNDCTEIGRLLHQTITLVLDRKLFGNYYNEWDLIELLNTKSEIEVCPL 541
S ++++ IC CT I L QT+++V D + G +W L + +++ E CPL
Sbjct: 220 HSQAVHIRPICRNAHCTSISWELRQTLSVVFDAFITGQGKKDWSLFRMF-SRTLTEACPL 278
Query: 542 AEISRIYVHGFG---PVDTSKIKP------TGVIQSNQLVY-----YDLATFNKTFNKIN 679
A S +YV G +T ++ P VI + Y +D A N + N +N
Sbjct: 279 ASQSLVYVDITGYSQDNETLEVSPPPTSTYQDVILGTRKTYAVYDLFDTAMINNSRN-LN 337
Query: 680 VKLSGVWPVDDINHLSRNCPF-QFTKFATGIGKVQGGIKATI-KSQSKNSIRVDYLESMP 853
++L P D + + PF ++ +G G +G + + S + V L+ +P
Sbjct: 338 IQLKWKRPPD---NEALPVPFLHAQRYVSGYGLQKGELSTLLYNSHPYRAFPVLLLDVVP 394
Query: 854 WY 859
WY
Sbjct: 395 WY 396
>sp|Q969N2|PIGT_HUMAN GPI transamidase component PIG-T precursor
(Phosphatidylinositol-glycan biosynthesis, class T
protein)
Length = 578
Score = 141 bits (356), Expect = 2e-33
Identities = 93/298 (31%), Positives = 141/298 (47%), Gaps = 14/298 (4%)
Frame = +2
Query: 8 WGISPNSLPSESAELWAWFEPFTKEIE--WNHLSHALSGLFCTSLNKLSNHKEVISPKWS 181
WG PS AELW WF+ +++ W LS+ LSG+FC SLN + + ++P S
Sbjct: 99 WGPPFLQAPS-GAELWVWFQDTVTDVDKSWKELSNVLSGIFCASLNFIDS-TNTVTPTAS 156
Query: 182 FSPEGLTHKFRNDSLIRKAVLSGEAICTENLTPWIKLLPCKKLAGLAQLLKPSRLFSSHF 361
F P GL + + +R AVL E +CTENLTPW KLLPC AGL+ LLK RLF + +
Sbjct: 157 FKPLGLAND-TDHYFLRYAVLpreVVCTENLTPWKKLLPCSSKAGLSVLLKADRLFHTSY 215
Query: 362 TSITMNVKKICLTNDCTEIGRLLHQTITLVLDRKLFGNYYNEWDLIELLNTKSEIEVCPL 541
S ++++ +C CT I L QT+++V D + G +W L + +++ E CPL
Sbjct: 216 HSQAVHIRPVCRNARCTSISWELRQTLSVVFDAFITGQGKKDWSLFRMF-SRTLTEPCPL 274
Query: 542 AEISRIYVH-----------GFGPVDTSKIKPTGVIQSNQLVYYDLATFNKTFNKINVKL 688
A SR+YV P T+ + + YDL N N+ +
Sbjct: 275 ASESRVYVDITTYNQDNETLEVHPPPTTTYQDVILGTRKTYAIYDLLDTAMINNSRNLNI 334
Query: 689 SGVWPVDDINHLSRNCPFQFTKFATGIGKVQGGIKATI-KSQSKNSIRVDYLESMPWY 859
W N ++ +G G +G + + + + V L+++PWY
Sbjct: 335 QLKWKRPPENEAPPVPFLHAQRYVSGYGLQKGELSTLLYNTHPYRAFPVLLLDTVPWY 392
>sp|P38875|GP16_YEAST GPI transamidase component GPI16 precursor
Length = 602
Score = 80.1 bits (196), Expect = 7e-15
Identities = 50/137 (36%), Positives = 76/137 (55%), Gaps = 8/137 (5%)
Frame = +2
Query: 8 WGISPNSLPSESA---ELWAWFEPFTKEI---EWNHLSHALSGLFCTSLNKLSNHKEVIS 169
WG P++ A ELW+ + +E W LS++LSGLFC+SLN + +
Sbjct: 103 WGQLPHAGKEAGASGVELWSQVQAMDQEQAFHNWKKLSNSLSGLFCSSLNFIDESRTTF- 161
Query: 170 PKWSFSPEGLTHKFRNDS--LIRKAVLSGEAICTENLTPWIKLLPCKKLAGLAQLLKPSR 343
P+ S++ + F + + +A L E ICTENLTP+IKLLP + +GL LL +
Sbjct: 162 PRRSYASDIGAPLFNSTEKLYLMRASLPNEPICTENLTPFIKLLPTRGKSGLTSLLDGHK 221
Query: 344 LFSSHFTSITMNVKKIC 394
LF S + SI++++ IC
Sbjct: 222 LFDSLWNSISLDIATIC 238
Score = 33.1 bits (74), Expect = 1.0
Identities = 11/41 (26%), Positives = 24/41 (58%)
Frame = +2
Query: 737 PFQFTKFATGIGKVQGGIKATIKSQSKNSIRVDYLESMPWY 859
P ++ TG G+ +GG++ + + +++ Y ES+PW+
Sbjct: 386 PIHVSRSLTGNGQDRGGMRIVFHNDNDTPVKLIYFESLPWF 426
>sp|O94380|GPI16_SCHPO GPI transamidase component PIG-T homolog precursor
Length = 545
Score = 65.9 bits (159), Expect = 1e-10
Identities = 46/133 (34%), Positives = 66/133 (49%), Gaps = 6/133 (4%)
Frame = +2
Query: 2 EKWGISPNS---LPSESAELWAWFEPFTKEIEWNHLSHALSGLFCTSLNKLSN---HKEV 163
E W SP++ E+WA+ W L++ LSGL C SLN + + ++
Sbjct: 98 ENWKESPDNGFYSGGLGFEVWAFMANDPSMKYWLKLTNQLSGLLCASLNYIDSSNTYQPQ 157
Query: 164 ISPKWSFSPEGLTHKFRNDSLIRKAVLSGEAICTENLTPWIKLLPCKKLAGLAQLLKPSR 343
+S SFS T F A L E +CTENL+P KLLPCK+ AG+A LL
Sbjct: 158 LSYPGSFSFSNNTQYF--------ASLPQEDVCTENLSPLFKLLPCKRKAGIASLLDSHL 209
Query: 344 LFSSHFTSITMNV 382
F + + S +++V
Sbjct: 210 FFDTDWHSFSIDV 222
>sp|Q03058|CAT_STRAG Chloramphenicol acetyltransferase (CAT)
Length = 215
Score = 32.0 bits (71), Expect = 2.3
Identities = 17/50 (34%), Positives = 26/50 (52%), Gaps = 1/50 (2%)
Frame = +2
Query: 581 VDTSKIKPTGVIQSNQLVYYD-LATFNKTFNKINVKLSGVWPVDDINHLS 727
V+ +K+ TG+ N+L Y+D L FNK K + +W D N +S
Sbjct: 61 VNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWTESDKNFIS 110
>sp|P36882|CAT4_STAAU Chloramphenicol acetyltransferase (CAT)
Length = 215
Score = 31.6 bits (70), Expect = 3.0
Identities = 27/102 (26%), Positives = 45/102 (44%), Gaps = 11/102 (10%)
Frame = +2
Query: 455 DRK-LFGNYYNEWDLIELLNTKSEIEVCPLAEISRIYVHGFGP---------VDTSKIKP 604
DRK F +Y+N+ + EI++ ++S+ + P V+ +K+
Sbjct: 12 DRKEYFEHYFNQQTTYSITK---EIDITLFKDMSKKKGYEIYPSLIYAIMEVVNKNKVFR 68
Query: 605 TGVIQSNQLVYYD-LATFNKTFNKINVKLSGVWPVDDINHLS 727
TG+ N+L Y+D L FNK K + +W D N S
Sbjct: 69 TGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWTESDNNFTS 110
>sp|P58742|AAAS_MOUSE Aladin (Adracalin)
Length = 546
Score = 30.8 bits (68), Expect = 5.2
Identities = 20/64 (31%), Positives = 30/64 (46%), Gaps = 1/64 (1%)
Frame = +2
Query: 593 KIKPTGVIQSNQLVYYDLATFNKTFNKINVKLSGVWPVDDINHLSRN-CPFQFTKFATGI 769
++ P G+IQ L TF+ +FNK LS W I H+ QF +F+ +
Sbjct: 440 ELLPCGIIQGEPGAQAQLITFHPSFNK-GALLSVCWSTGRITHIPLYFVNAQFPRFSPVL 498
Query: 770 GKVQ 781
G+ Q
Sbjct: 499 GRAQ 502
>sp|P06135|CAT3_STAAU Chloramphenicol acetyltransferase (CAT)
Length = 215
Score = 30.8 bits (68), Expect = 5.2
Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = +2
Query: 581 VDTSKIKPTGVIQSNQLVYYD-LATFNKTFNKINVKLSGVWPVDDINHLS 727
V+ +K+ TG+ N+L Y+D L FNK K + +W D N S
Sbjct: 61 VNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWTESDNNFTS 110
>sp|P00486|CAT2_STAAU Chloramphenicol acetyltransferase (CAT)
Length = 215
Score = 30.8 bits (68), Expect = 5.2
Identities = 17/50 (34%), Positives = 25/50 (50%), Gaps = 1/50 (2%)
Frame = +2
Query: 581 VDTSKIKPTGVIQSNQLVYYD-LATFNKTFNKINVKLSGVWPVDDINHLS 727
V+ +K+ TG+ N+L Y+D L FNK K + +W D N S
Sbjct: 61 VNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWTESDNNFTS 110
>sp|Q8XUZ8|RPOB_RALSO DNA-directed RNA polymerase beta chain (RNAP beta subunit)
(Transcriptase beta chain) (RNA polymerase beta subunit)
Length = 1368
Score = 30.8 bits (68), Expect = 5.2
Identities = 18/44 (40%), Positives = 23/44 (52%)
Frame = +2
Query: 416 IGRLLHQTITLVLDRKLFGNYYNEWDLIELLNTKSEIEVCPLAE 547
IG +L RKL G YNE +E L++ S+ EV LAE
Sbjct: 1147 IGNMLKAQAKAAEMRKLLGQIYNESGKVEDLDSLSDAEVLELAE 1190
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 94,661,761
Number of Sequences: 369166
Number of extensions: 1783377
Number of successful extensions: 4354
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4234
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4344
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8742211660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)