Planaria EST Database


DrC_01346

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01346
         (906 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P25386|USO1_YEAST  Intracellular protein transport protei...    40   0.009
sp|Q898C7|CLPB_CLOTE  Chaperone clpB                               40   0.012
sp|O88597|BCN1_MOUSE  Beclin-1 (Coiled-coil myosin-like BCL2...    40   0.012
sp|Q97WH0|RAD50_SULSO  DNA double-strand break repair rad50 ...    39   0.026
sp|Q5SX40|MYH1_MOUSE  Myosin-1 (Myosin heavy chain, skeletal...    38   0.034
sp|Q58651|YC54_METJA  Hypothetical protein MJ1254                  38   0.045
sp|Q15075|EEA1_HUMAN  Early endosome antigen 1 (Endosome-ass...    37   0.058
sp|P12845|MYO2_CAEEL  Myosin-2 (Myosin heavy chain C) (MHC C)      37   0.058
sp|P47597|CLPB_MYCGE  Chaperone clpB                               37   0.058
sp|P27895|CIN8_YEAST  Kinesin-like protein CIN8                    37   0.058
>sp|P25386|USO1_YEAST Intracellular protein transport protein USO1 (Int-1)
          Length = 1790

 Score = 40.0 bits (92), Expect = 0.009
 Identities = 28/130 (21%), Positives = 63/130 (48%), Gaps = 4/130 (3%)
 Frame = +2

Query: 56   KALFDEGFPMDTNVDAEEVDRLKNELDKSERCRQTLELRFAETKDDSEK----NKQLIQS 223
            + L +EG    T   +E+++ L++EL + +   +        T+ + EK    N +L++ 
Sbjct: 1369 RKLLNEGSSTITQEYSEKINTLEDELIRLQNENELKAKEIDNTRSELEKVSLSNDELLEE 1428

Query: 224  QIKEIQSLKDQISEYRTKYHREQDRGDYYKKDFDKLYRDLKEIDKLSVGLGKYISESVER 403
            +   I+SL+D+I  Y+ K  R  ++    ++D  +    LKE  + +      + E +++
Sbjct: 1429 KQNTIKSLQDEILSYKDKITRNDEKLLSIERDNKRDLESLKEQLRAAQESKAKVEEGLKK 1488

Query: 404  FKKTDKVDKA 433
             ++    +KA
Sbjct: 1489 LEEESSKEKA 1498

 Score = 31.6 bits (70), Expect = 3.2
 Identities = 30/127 (23%), Positives = 64/127 (50%), Gaps = 13/127 (10%)
 Frame = +2

Query: 89   TNVDAEEVDRLKNELDKSERCRQTLELRFAET----KDDSEKNKQL---IQSQIKEIQSL 247
            +N+   E+D L  ++ + ++  +T E    E+    + ++ K K+L      + KE+  L
Sbjct: 1218 SNLKKSEIDALNLQIKELKKKNETNEASLLESIKSVESETVKIKELQDECNFKEKEVSEL 1277

Query: 248  KDQI---SEYRTKYHREQDRGDYYKKDFDKLYRDLK-EIDKL-SVGLGKYISES-VERFK 409
            +D++    +  +KY   Q   +  K++ D    +LK +++K+ ++   K  SES + R K
Sbjct: 1278 EDKLKASEDKNSKYLELQKESEKIKEELDAKTTELKIQLEKITNLSKAKEKSESELSRLK 1337

Query: 410  KTDKVDK 430
            KT   ++
Sbjct: 1338 KTSSEER 1344
>sp|Q898C7|CLPB_CLOTE Chaperone clpB
          Length = 865

 Score = 39.7 bits (91), Expect = 0.012
 Identities = 37/139 (26%), Positives = 65/139 (46%), Gaps = 15/139 (10%)
 Frame = +2

Query: 29  YIRELAMGNKA--LFDEGFPMDTNVDAEEVDRLKNELDKSERCRQTLEL-RFAETKDDSE 199
           YI +  + +KA  L DE   M       E+D L  ELD  +R    +E+ + A  K+   
Sbjct: 384 YITDRYLPDKAIDLIDEAGAMIRT----EIDSLPTELDSIKRKIFQMEIEKEALAKEKDS 439

Query: 200 KNKQLIQSQIKEIQSLKDQISEYRTKYHREQD----------RGDYYKKDFDKLYR--DL 343
           ++K+ ++   KE+ +LK++  E   KY +E++          + D  K   +K  R  DL
Sbjct: 440 RSKERLEDLEKELSNLKEKDKEMTAKYEKEKEQIINMRNLKQKLDEVKGQLEKAEREYDL 499

Query: 344 KEIDKLSVGLGKYISESVE 400
            ++ +L  G+   I   +E
Sbjct: 500 NKVAELKYGIIPGIKSQIE 518
>sp|O88597|BCN1_MOUSE Beclin-1 (Coiled-coil myosin-like BCL2-interacting protein)
          Length = 448

 Score = 39.7 bits (91), Expect = 0.012
 Identities = 25/109 (22%), Positives = 55/109 (50%), Gaps = 5/109 (4%)
 Frame = +2

Query: 107 EVDRLKNELDKSERCRQTLELRFAETK-----DDSEKNKQLIQSQIKEIQSLKDQISEYR 271
           E+    NE D  +  R+  EL   E +     +D EKN++++   ++++Q+  +++ +  
Sbjct: 165 EILEQMNEDDSEQLQRELKELALEEERLIQELEDVEKNRKVVAENLEKVQAEAERLDQEE 224

Query: 272 TKYHREQDRGDYYKKDFDKLYRDLKEIDKLSVGLGKYISESVERFKKTD 418
            +YHRE      +K+   +L  +LK ++       +Y    +++ KKT+
Sbjct: 225 AQYHREYSE---FKRQQLELDDELKSVENQV----RYAQIQLDKLKKTN 266
>sp|Q97WH0|RAD50_SULSO DNA double-strand break repair rad50 ATPase
          Length = 864

 Score = 38.5 bits (88), Expect = 0.026
 Identities = 29/109 (26%), Positives = 51/109 (46%), Gaps = 1/109 (0%)
 Frame = +2

Query: 107 EVDRLKNELDKSERCRQTLELRFAETKDDSEKNKQLIQSQIKEIQSLKDQISEYRTKYHR 286
           E+DR++ + +  ++  +    R  E K D EK +  I++  K I+ +KDQ  EY      
Sbjct: 177 ELDRIEQDYNNFKKTVEEKRARVLELKKDKEKLEDEIKNLEKRIKDIKDQFDEY------ 230

Query: 287 EQDRGDYYKKDFDKLYRDLKEIDKLSVGLGKYISESVERFKK-TDKVDK 430
           E+ R  Y K         L    K+  G    ++ S+E  +K T+ +D+
Sbjct: 231 EKKRNQYLK---------LTTTLKIKEGELNELNRSIEELRKQTENMDQ 270
>sp|Q5SX40|MYH1_MOUSE Myosin-1 (Myosin heavy chain, skeletal muscle, adult 1)
          Length = 1942

 Score = 38.1 bits (87), Expect = 0.034
 Identities = 32/119 (26%), Positives = 55/119 (46%), Gaps = 7/119 (5%)
 Frame = +2

Query: 101  AEEVDRLKNELDKSERCRQTLELRFAETK---DDSEKNKQLIQSQI----KEIQSLKDQI 259
            A+ V  L  ++D  +R +Q LE   +E K   DD   N ++I        K  ++L+DQ+
Sbjct: 1204 ADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMEVISKSKGNLEKMCRTLEDQV 1263

Query: 260  SEYRTKYHREQDRGDYYKKDFDKLYRDLKEIDKLSVGLGKYISESVERFKKTDKVDKAV 436
            SE +TK   +Q                 + I++L+   G+  +ES E  ++ D+ D  V
Sbjct: 1264 SELKTKEEEQQ-----------------RLINELTAQRGRLQTESGEYSRQLDEKDSLV 1305

 Score = 30.4 bits (67), Expect = 7.1
 Identities = 33/133 (24%), Positives = 57/133 (42%)
 Frame = +2

Query: 20   DLDYIRELAMGNKALFDEGFPMDTNVDAEEVDRLKNELDKSERCRQTLELRFAETKDDSE 199
            DL+  +    G+  L  E   MD   D +++D    +L K E     L+ +     +D +
Sbjct: 1054 DLERAKRKLEGDLKLAQES-TMDVENDKQQLDE---KLKKKEFEMSNLQSKI----EDEQ 1105

Query: 200  KNKQLIQSQIKEIQSLKDQISEYRTKYHREQDRGDYYKKDFDKLYRDLKEIDKLSVGLGK 379
                 +Q +IKE+Q+   +I E   +   E+      +K    L R+L+EI +     G 
Sbjct: 1106 ALGMQLQKKIKELQA---RIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGG 1162

Query: 380  YISESVERFKKTD 418
              S  +E  KK +
Sbjct: 1163 ATSAQIEMNKKRE 1175
>sp|Q58651|YC54_METJA Hypothetical protein MJ1254
          Length = 469

 Score = 37.7 bits (86), Expect = 0.045
 Identities = 24/102 (23%), Positives = 51/102 (50%)
 Frame = +2

Query: 107 EVDRLKNELDKSERCRQTLELRFAETKDDSEKNKQLIQSQIKEIQSLKDQISEYRTKYHR 286
           E+  LKN+L + E   + L ++  + +D   K  + + ++ + I  L ++ISEY ++  +
Sbjct: 288 ELTSLKNKLKEKEEEIKNLAIKIKDLEDKLSKANKNLLNKDEIISVLNERISEYESQIQK 347

Query: 287 EQDRGDYYKKDFDKLYRDLKEIDKLSVGLGKYISESVERFKK 412
             D    YK+  + L + ++ + K +  L   + E  +  KK
Sbjct: 348 LLDENIIYKEKIESLNKYIETLKKENDKLKDKVRELSDIAKK 389
>sp|Q15075|EEA1_HUMAN Early endosome antigen 1 (Endosome-associated protein p162) (Zinc
            finger FYVE domain-containing protein 2)
          Length = 1411

 Score = 37.4 bits (85), Expect = 0.058
 Identities = 32/142 (22%), Positives = 60/142 (42%), Gaps = 25/142 (17%)
 Frame = +2

Query: 83   MDTNVDAEEVDRLKNELDKSERCRQTLELRFA----ETKDDSEKNKQLIQSQIKEIQSLK 250
            +D    +E ++ +K +L K E  +Q L+  F     ETK   E+    IQ+ + E+Q +K
Sbjct: 786  LDLQKKSEALESIKQKLTKQEEEKQILKQDFETLSQETKIQHEELNNRIQTTVTELQKVK 845

Query: 251  ---------------------DQISEYRTKYHREQDRGDYYKKDFDKLYRDLKEIDKLSV 367
                                 D +   ++++ +E  +G     D +K  ++LK   +L V
Sbjct: 846  MEKEALMTELSTVKDKLSKVSDSLKNSKSEFEKENQKGKAAILDLEKTCKELKH--QLQV 903

Query: 368  GLGKYISESVERFKKTDKVDKA 433
             +   + E  E  K  +K  +A
Sbjct: 904  QMENTLKEQKELKKSLEKEKEA 925
>sp|P12845|MYO2_CAEEL Myosin-2 (Myosin heavy chain C) (MHC C)
          Length = 1947

 Score = 37.4 bits (85), Expect = 0.058
 Identities = 34/113 (30%), Positives = 54/113 (47%), Gaps = 7/113 (6%)
 Frame = +2

Query: 95   VDAEEVDRLKNELDKSERCRQTLELRFAETKD-DSEKNKQLI-----QSQI-KEIQSLKD 253
            V  +E + L  +L+  ER R+ +E    E ++ ++E N Q +     +SQ+  EI  L  
Sbjct: 1696 VAKQEQEELIVKLEALERARRVVESSVKEHQEHNNELNSQNVALAAAKSQLDNEIALLNS 1755

Query: 254  QISEYRTKYHREQDRGDYYKKDFDKLYRDLKEIDKLSVGLGKYISESVERFKK 412
             I+E  T+    +DRG     D  KL  DL+   +         S+ +ERFKK
Sbjct: 1756 DIAEAHTELSASEDRGRRAASDAAKLAEDLRHEQEQ--------SQQLERFKK 1800
>sp|P47597|CLPB_MYCGE Chaperone clpB
          Length = 714

 Score = 37.4 bits (85), Expect = 0.058
 Identities = 37/126 (29%), Positives = 58/126 (46%), Gaps = 5/126 (3%)
 Frame = +2

Query: 29  YIRELAMGNKA--LFDEGFP-MDTNVDAEEV--DRLKNELDKSERCRQTLELRFAETKDD 193
           YI E  + +KA  L DE    + T + +E V  D LK E+         LE  +A  K D
Sbjct: 236 YINERNLPDKAIDLIDEAAAKIKTEMSSEPVAIDSLKREIIN-------LETEYAALKQD 288

Query: 194 SEKNKQLIQSQIKEIQSLKDQISEYRTKYHREQDRGDYYKKDFDKLYRDLKEIDKLSVGL 373
            E + +  QS+ + ++ LK Q+   + K     +     K DF+ + +  KEI++    L
Sbjct: 289 KENDNK--QSKKEYLEKLKKQLDALKQKRDSLINEWKKEKADFENINKLKKEIEEFQTKL 346

Query: 374 GKYISE 391
             Y SE
Sbjct: 347 ETYQSE 352
>sp|P27895|CIN8_YEAST Kinesin-like protein CIN8
          Length = 1038

 Score = 37.4 bits (85), Expect = 0.058
 Identities = 24/87 (27%), Positives = 48/87 (55%)
 Frame = +2

Query: 107 EVDRLKNELDKSERCRQTLELRFAETKDDSEKNKQLIQSQIKEIQSLKDQISEYRTKYHR 286
           +++  KNE+ + +R  ++L  + A    D  K+K+ IQSQ  +I+SLK  I   R +  +
Sbjct: 567 DLESYKNEVQECKREIESLTSKNALLVKDKLKSKETIQSQNCQIESLKTTIDHLRAQLDK 626

Query: 287 EQDRGDYYKKDFDKLYRDLKEIDKLSV 367
            Q + +    DF+   + L E+ ++++
Sbjct: 627 -QHKTEIEISDFNNKLQKLTEVMQMAL 652
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,295,678
Number of Sequences: 369166
Number of extensions: 1495857
Number of successful extensions: 5835
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5414
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5812
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9174518830
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)