Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_003_K07
(906 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P25386|USO1_YEAST Intracellular protein transport protei... 40 0.009
sp|Q898C7|CLPB_CLOTE Chaperone clpB 40 0.012
sp|O88597|BCN1_MOUSE Beclin-1 (Coiled-coil myosin-like BCL2... 40 0.012
sp|Q97WH0|RAD50_SULSO DNA double-strand break repair rad50 ... 39 0.026
sp|Q5SX40|MYH1_MOUSE Myosin-1 (Myosin heavy chain, skeletal... 38 0.034
sp|Q58651|YC54_METJA Hypothetical protein MJ1254 38 0.045
sp|Q15075|EEA1_HUMAN Early endosome antigen 1 (Endosome-ass... 37 0.058
sp|P12845|MYO2_CAEEL Myosin-2 (Myosin heavy chain C) (MHC C) 37 0.058
sp|P47597|CLPB_MYCGE Chaperone clpB 37 0.058
sp|P27895|CIN8_YEAST Kinesin-like protein CIN8 37 0.058
>sp|P25386|USO1_YEAST Intracellular protein transport protein USO1 (Int-1)
Length = 1790
Score = 40.0 bits (92), Expect = 0.009
Identities = 28/130 (21%), Positives = 63/130 (48%), Gaps = 4/130 (3%)
Frame = +2
Query: 56 KALFDEGFPMDTNVDAEEVDRLKNELDKSERCRQTLELRFAETKDDSEK----NKQLIQS 223
+ L +EG T +E+++ L++EL + + + T+ + EK N +L++
Sbjct: 1369 RKLLNEGSSTITQEYSEKINTLEDELIRLQNENELKAKEIDNTRSELEKVSLSNDELLEE 1428
Query: 224 QIKEIQSLKDQISEYRTKYHREQDRGDYYKKDFDKLYRDLKEIDKLSVGLGKYISESVER 403
+ I+SL+D+I Y+ K R ++ ++D + LKE + + + E +++
Sbjct: 1429 KQNTIKSLQDEILSYKDKITRNDEKLLSIERDNKRDLESLKEQLRAAQESKAKVEEGLKK 1488
Query: 404 FKKTDKVDKA 433
++ +KA
Sbjct: 1489 LEEESSKEKA 1498
Score = 31.6 bits (70), Expect = 3.2
Identities = 30/127 (23%), Positives = 64/127 (50%), Gaps = 13/127 (10%)
Frame = +2
Query: 89 TNVDAEEVDRLKNELDKSERCRQTLELRFAET----KDDSEKNKQL---IQSQIKEIQSL 247
+N+ E+D L ++ + ++ +T E E+ + ++ K K+L + KE+ L
Sbjct: 1218 SNLKKSEIDALNLQIKELKKKNETNEASLLESIKSVESETVKIKELQDECNFKEKEVSEL 1277
Query: 248 KDQI---SEYRTKYHREQDRGDYYKKDFDKLYRDLK-EIDKL-SVGLGKYISES-VERFK 409
+D++ + +KY Q + K++ D +LK +++K+ ++ K SES + R K
Sbjct: 1278 EDKLKASEDKNSKYLELQKESEKIKEELDAKTTELKIQLEKITNLSKAKEKSESELSRLK 1337
Query: 410 KTDKVDK 430
KT ++
Sbjct: 1338 KTSSEER 1344
>sp|Q898C7|CLPB_CLOTE Chaperone clpB
Length = 865
Score = 39.7 bits (91), Expect = 0.012
Identities = 37/139 (26%), Positives = 65/139 (46%), Gaps = 15/139 (10%)
Frame = +2
Query: 29 YIRELAMGNKA--LFDEGFPMDTNVDAEEVDRLKNELDKSERCRQTLEL-RFAETKDDSE 199
YI + + +KA L DE M E+D L ELD +R +E+ + A K+
Sbjct: 384 YITDRYLPDKAIDLIDEAGAMIRT----EIDSLPTELDSIKRKIFQMEIEKEALAKEKDS 439
Query: 200 KNKQLIQSQIKEIQSLKDQISEYRTKYHREQD----------RGDYYKKDFDKLYR--DL 343
++K+ ++ KE+ +LK++ E KY +E++ + D K +K R DL
Sbjct: 440 RSKERLEDLEKELSNLKEKDKEMTAKYEKEKEQIINMRNLKQKLDEVKGQLEKAEREYDL 499
Query: 344 KEIDKLSVGLGKYISESVE 400
++ +L G+ I +E
Sbjct: 500 NKVAELKYGIIPGIKSQIE 518
>sp|O88597|BCN1_MOUSE Beclin-1 (Coiled-coil myosin-like BCL2-interacting protein)
Length = 448
Score = 39.7 bits (91), Expect = 0.012
Identities = 25/109 (22%), Positives = 55/109 (50%), Gaps = 5/109 (4%)
Frame = +2
Query: 107 EVDRLKNELDKSERCRQTLELRFAETK-----DDSEKNKQLIQSQIKEIQSLKDQISEYR 271
E+ NE D + R+ EL E + +D EKN++++ ++++Q+ +++ +
Sbjct: 165 EILEQMNEDDSEQLQRELKELALEEERLIQELEDVEKNRKVVAENLEKVQAEAERLDQEE 224
Query: 272 TKYHREQDRGDYYKKDFDKLYRDLKEIDKLSVGLGKYISESVERFKKTD 418
+YHRE +K+ +L +LK ++ +Y +++ KKT+
Sbjct: 225 AQYHREYSE---FKRQQLELDDELKSVENQV----RYAQIQLDKLKKTN 266
>sp|Q97WH0|RAD50_SULSO DNA double-strand break repair rad50 ATPase
Length = 864
Score = 38.5 bits (88), Expect = 0.026
Identities = 29/109 (26%), Positives = 51/109 (46%), Gaps = 1/109 (0%)
Frame = +2
Query: 107 EVDRLKNELDKSERCRQTLELRFAETKDDSEKNKQLIQSQIKEIQSLKDQISEYRTKYHR 286
E+DR++ + + ++ + R E K D EK + I++ K I+ +KDQ EY
Sbjct: 177 ELDRIEQDYNNFKKTVEEKRARVLELKKDKEKLEDEIKNLEKRIKDIKDQFDEY------ 230
Query: 287 EQDRGDYYKKDFDKLYRDLKEIDKLSVGLGKYISESVERFKK-TDKVDK 430
E+ R Y K L K+ G ++ S+E +K T+ +D+
Sbjct: 231 EKKRNQYLK---------LTTTLKIKEGELNELNRSIEELRKQTENMDQ 270
>sp|Q5SX40|MYH1_MOUSE Myosin-1 (Myosin heavy chain, skeletal muscle, adult 1)
Length = 1942
Score = 38.1 bits (87), Expect = 0.034
Identities = 32/119 (26%), Positives = 55/119 (46%), Gaps = 7/119 (5%)
Frame = +2
Query: 101 AEEVDRLKNELDKSERCRQTLELRFAETK---DDSEKNKQLIQSQI----KEIQSLKDQI 259
A+ V L ++D +R +Q LE +E K DD N ++I K ++L+DQ+
Sbjct: 1204 ADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNMEVISKSKGNLEKMCRTLEDQV 1263
Query: 260 SEYRTKYHREQDRGDYYKKDFDKLYRDLKEIDKLSVGLGKYISESVERFKKTDKVDKAV 436
SE +TK +Q + I++L+ G+ +ES E ++ D+ D V
Sbjct: 1264 SELKTKEEEQQ-----------------RLINELTAQRGRLQTESGEYSRQLDEKDSLV 1305
Score = 30.4 bits (67), Expect = 7.1
Identities = 33/133 (24%), Positives = 57/133 (42%)
Frame = +2
Query: 20 DLDYIRELAMGNKALFDEGFPMDTNVDAEEVDRLKNELDKSERCRQTLELRFAETKDDSE 199
DL+ + G+ L E MD D +++D +L K E L+ + +D +
Sbjct: 1054 DLERAKRKLEGDLKLAQES-TMDVENDKQQLDE---KLKKKEFEMSNLQSKI----EDEQ 1105
Query: 200 KNKQLIQSQIKEIQSLKDQISEYRTKYHREQDRGDYYKKDFDKLYRDLKEIDKLSVGLGK 379
+Q +IKE+Q+ +I E + E+ +K L R+L+EI + G
Sbjct: 1106 ALGMQLQKKIKELQA---RIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGG 1162
Query: 380 YISESVERFKKTD 418
S +E KK +
Sbjct: 1163 ATSAQIEMNKKRE 1175
>sp|Q58651|YC54_METJA Hypothetical protein MJ1254
Length = 469
Score = 37.7 bits (86), Expect = 0.045
Identities = 24/102 (23%), Positives = 51/102 (50%)
Frame = +2
Query: 107 EVDRLKNELDKSERCRQTLELRFAETKDDSEKNKQLIQSQIKEIQSLKDQISEYRTKYHR 286
E+ LKN+L + E + L ++ + +D K + + ++ + I L ++ISEY ++ +
Sbjct: 288 ELTSLKNKLKEKEEEIKNLAIKIKDLEDKLSKANKNLLNKDEIISVLNERISEYESQIQK 347
Query: 287 EQDRGDYYKKDFDKLYRDLKEIDKLSVGLGKYISESVERFKK 412
D YK+ + L + ++ + K + L + E + KK
Sbjct: 348 LLDENIIYKEKIESLNKYIETLKKENDKLKDKVRELSDIAKK 389
>sp|Q15075|EEA1_HUMAN Early endosome antigen 1 (Endosome-associated protein p162) (Zinc
finger FYVE domain-containing protein 2)
Length = 1411
Score = 37.4 bits (85), Expect = 0.058
Identities = 32/142 (22%), Positives = 60/142 (42%), Gaps = 25/142 (17%)
Frame = +2
Query: 83 MDTNVDAEEVDRLKNELDKSERCRQTLELRFA----ETKDDSEKNKQLIQSQIKEIQSLK 250
+D +E ++ +K +L K E +Q L+ F ETK E+ IQ+ + E+Q +K
Sbjct: 786 LDLQKKSEALESIKQKLTKQEEEKQILKQDFETLSQETKIQHEELNNRIQTTVTELQKVK 845
Query: 251 ---------------------DQISEYRTKYHREQDRGDYYKKDFDKLYRDLKEIDKLSV 367
D + ++++ +E +G D +K ++LK +L V
Sbjct: 846 MEKEALMTELSTVKDKLSKVSDSLKNSKSEFEKENQKGKAAILDLEKTCKELKH--QLQV 903
Query: 368 GLGKYISESVERFKKTDKVDKA 433
+ + E E K +K +A
Sbjct: 904 QMENTLKEQKELKKSLEKEKEA 925
>sp|P12845|MYO2_CAEEL Myosin-2 (Myosin heavy chain C) (MHC C)
Length = 1947
Score = 37.4 bits (85), Expect = 0.058
Identities = 34/113 (30%), Positives = 54/113 (47%), Gaps = 7/113 (6%)
Frame = +2
Query: 95 VDAEEVDRLKNELDKSERCRQTLELRFAETKD-DSEKNKQLI-----QSQI-KEIQSLKD 253
V +E + L +L+ ER R+ +E E ++ ++E N Q + +SQ+ EI L
Sbjct: 1696 VAKQEQEELIVKLEALERARRVVESSVKEHQEHNNELNSQNVALAAAKSQLDNEIALLNS 1755
Query: 254 QISEYRTKYHREQDRGDYYKKDFDKLYRDLKEIDKLSVGLGKYISESVERFKK 412
I+E T+ +DRG D KL DL+ + S+ +ERFKK
Sbjct: 1756 DIAEAHTELSASEDRGRRAASDAAKLAEDLRHEQEQ--------SQQLERFKK 1800
>sp|P47597|CLPB_MYCGE Chaperone clpB
Length = 714
Score = 37.4 bits (85), Expect = 0.058
Identities = 37/126 (29%), Positives = 58/126 (46%), Gaps = 5/126 (3%)
Frame = +2
Query: 29 YIRELAMGNKA--LFDEGFP-MDTNVDAEEV--DRLKNELDKSERCRQTLELRFAETKDD 193
YI E + +KA L DE + T + +E V D LK E+ LE +A K D
Sbjct: 236 YINERNLPDKAIDLIDEAAAKIKTEMSSEPVAIDSLKREIIN-------LETEYAALKQD 288
Query: 194 SEKNKQLIQSQIKEIQSLKDQISEYRTKYHREQDRGDYYKKDFDKLYRDLKEIDKLSVGL 373
E + + QS+ + ++ LK Q+ + K + K DF+ + + KEI++ L
Sbjct: 289 KENDNK--QSKKEYLEKLKKQLDALKQKRDSLINEWKKEKADFENINKLKKEIEEFQTKL 346
Query: 374 GKYISE 391
Y SE
Sbjct: 347 ETYQSE 352
>sp|P27895|CIN8_YEAST Kinesin-like protein CIN8
Length = 1038
Score = 37.4 bits (85), Expect = 0.058
Identities = 24/87 (27%), Positives = 48/87 (55%)
Frame = +2
Query: 107 EVDRLKNELDKSERCRQTLELRFAETKDDSEKNKQLIQSQIKEIQSLKDQISEYRTKYHR 286
+++ KNE+ + +R ++L + A D K+K+ IQSQ +I+SLK I R + +
Sbjct: 567 DLESYKNEVQECKREIESLTSKNALLVKDKLKSKETIQSQNCQIESLKTTIDHLRAQLDK 626
Query: 287 EQDRGDYYKKDFDKLYRDLKEIDKLSV 367
Q + + DF+ + L E+ ++++
Sbjct: 627 -QHKTEIEISDFNNKLQKLTEVMQMAL 652
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 86,295,678
Number of Sequences: 369166
Number of extensions: 1495857
Number of successful extensions: 5835
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5414
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5812
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9174518830
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)