Planaria EST Database


DrC_01177

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01177
         (921 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O94989|ARHGF_HUMAN  Rho guanine nucleotide exchange facto...   111   2e-24
sp|Q12774|ARHG5_HUMAN  Rho guanine nucleotide exchange facto...    91   5e-18
sp|P51862|ROM2_YEAST  RHO1 GDP-GTP exchange protein 2              59   1e-08
sp|P53046|ROM1_YEAST  RHO1 GDP-GTP exchange protein 1 (Prote...    57   6e-08
sp|Q9H8V3|ECT2_HUMAN  ECT2 protein (Epithelial cell transfor...    49   3e-05
sp|Q92888|ARHG1_HUMAN  Rho guanine nucleotide exchange facto...    47   6e-05
sp|Q07139|ECT2_MOUSE  ECT2 protein (Epithelial cell transfor...    46   1e-04
sp|Q9Z1I6|ARHG1_RAT  Rho guanine nucleotide exchange factor ...    45   2e-04
sp|Q9ULL1|PKHG1_HUMAN  Pleckstrin homology domain containing...    44   5e-04
sp|Q61210|ARHG1_MOUSE  Rho guanine nucleotide exchange facto...    44   8e-04
>sp|O94989|ARHGF_HUMAN Rho guanine nucleotide exchange factor 15 (Vsm-RhoGEF)
          Length = 841

 Score =  111 bits (278), Expect = 2e-24
 Identities = 86/291 (29%), Positives = 140/291 (48%), Gaps = 10/291 (3%)
 Frame = +3

Query: 9    VRSKGLAKHMKKEEIKYQESQFELISSEASYNRSLSVLVDTFYNSPQLSLTETEYFKPII 188
            V++ GL   +  +E + QES FE+++SEASY RSL +L DTF  S  L  T T       
Sbjct: 402  VQASGLLDTLSPQERRMQESLFEVVTSEASYLRSLRLLTDTFVLSQALRDTLT------- 454

Query: 189  RPYQKEHLFSNILKIYAVSSSFLADLEHRFCESAKIIDLSDIVYKYSIKEFDVYVPYIQN 368
             P     LFSN+ ++  VS  FLA L  R   S  I DL D+V+ +++  F VYV Y++N
Sbjct: 455  -PRDHHTLFSNVQRVQGVSERFLATLLSRVRSSPHISDLCDVVHAHAVGPFSVYVDYVRN 513

Query: 369  QRFQSEAYAELKKDPNT--AKVFSELESQPECCFLNLDSFXXXXXXXXXXXXXXXKAMKG 542
            Q++Q E Y+ L  D N   +     L+S P+C  L L SF               + +  
Sbjct: 514  QQYQEETYSRL-MDTNVRFSAELRRLQSLPKCERLPLPSFLLLPFQRITRLRMLLQNILR 572

Query: 543  NIQTNQKHICESIEKAISSMDKIIQKCSENKCHIDERKRMMEIYSCLDFKPGQNI----- 707
              +       E+ +KA+ ++ KII++CS     + + + ++ +   L F   + +     
Sbjct: 573  QTEEGSSR-QENAQKALGAVSKIIERCSAEVGRMKQTEELIRLTQRLRFHKVKALPLVSW 631

Query: 708  ---REPLGKLIHEGEVRRTSKNSTKRQFKDYWFILTSEFLLMTKKSKGKEM 851
                E  G+L   G  R     +++ +F     +L S+ LL+T+   G+ +
Sbjct: 632  SRRLEFQGELTELGCRRGGVLFASRPRFTPLCLLLFSDLLLITQPKSGQRL 682
>sp|Q12774|ARHG5_HUMAN Rho guanine nucleotide exchange factor 5 (Guanine nucleotide
           regulatory protein TIM) (Oncogene TIM) (p60 TIM)
           (Transforming immortalized mammary oncogene)
          Length = 519

 Score = 90.9 bits (224), Expect = 5e-18
 Identities = 65/229 (28%), Positives = 110/229 (48%), Gaps = 1/229 (0%)
 Frame = +3

Query: 9   VRSKGLAKHMKKEEIKYQESQFELISSEASYNRSLSVLVDTFYNSPQLSLTETEYFKPII 188
           VR+  +   M  E+ K QE +FELI SEASY RSL++ VD F  S  L  T        +
Sbjct: 81  VRNSTVLLSMTHEDQKLQEVKFELIVSEASYLRSLNIAVDHFQLSTSLRAT--------L 132

Query: 189 RPYQKEHLFSNILKIYAVSSSFLADLEHRFCESAKIIDLSDIVYKYSIKEFDVYVPYIQN 368
              + + LFS +  +  VS++FL+DLE  F  +     + D+V  ++     VY+PY+ N
Sbjct: 133 SNQEHQWLFSRLQDVRDVSATFLSDLEENFENNIFSFQVCDVVLNHAPDFRRVYLPYVTN 192

Query: 369 QRFQSEAYAEL-KKDPNTAKVFSELESQPECCFLNLDSFXXXXXXXXXXXXXXXKAMKGN 545
           Q +Q   +  L   + N  +V  +LES P C  L+L SF               + +   
Sbjct: 193 QTYQERTFQSLMNSNSNFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKR 252

Query: 546 IQTNQKHICESIEKAISSMDKIIQKCSENKCHIDERKRMMEIYSCLDFK 692
            Q       E+  KA  +++++I+ C+ N   +   + ++ +   ++F+
Sbjct: 253 TQPGSSEEAEA-TKAHHALEQLIRDCNNNVQSMRRTEELIYLSQKIEFE 300
>sp|P51862|ROM2_YEAST RHO1 GDP-GTP exchange protein 2
          Length = 1356

 Score = 59.3 bits (142), Expect = 1e-08
 Identities = 63/272 (23%), Positives = 116/272 (42%), Gaps = 5/272 (1%)
 Frame = +3

Query: 30   KHMKKEEIKYQESQFELISSEASYNRSLSVLVDTFYNSPQLSLTETEYFKPIIRPYQKEH 209
            +++ K+EIK QE+ +E+  +E ++ +SL +  DTF  +    L ET      IR    +H
Sbjct: 651  ENLPKKEIKRQEAIYEVYITEKNFVKSLEITRDTFMKT----LAETNIISADIRKNFIKH 706

Query: 210  LFSNILKIYAVSSSFLADLEHRFCESAKIIDLSDIVYKYSIKEFDVYVPYIQNQRFQSEA 389
            +F++I  IY+V+  FL  L  R   S  +  + DIV ++ I  F+ +V Y+ ++ + ++ 
Sbjct: 707  VFAHINDIYSVNRRFLKALTDRQRSSPVVRGIGDIVLRF-IPFFEPFVSYVASRPY-AKY 764

Query: 390  YAELKKDPNTAKVFSELESQPECCFLNLDSFXXXXXXXXXXXXXXXK-AMKGNIQTNQKH 566
              E ++  N      + +         +DSF               K  MK       K 
Sbjct: 765  LIETQRSVNPYFARFDDDMMSSSLRHGIDSFLSQGVSRPGRYMLLVKEIMKSTDPEKDKS 824

Query: 567  ICESIEKAISSMDKIIQKCSENKCHIDERKRMMEIYSCLDFK---PGQNIREPLGKLIHE 737
              E + KA+ ++   +++  +      +R  +  +   + FK       + +   K+ HE
Sbjct: 825  DYEDLSKAMDALRDFMKRIDQASGAAQDRHDVKLLKQKILFKNEYVNLGLNDERRKIKHE 884

Query: 738  GEV-RRTSKNSTKRQFKDYWFILTSEFLLMTK 830
            G + R+    S      D  F L    LL  K
Sbjct: 885  GILSRKELSKSDGTVVGDIQFYLLDNMLLFLK 916
>sp|P53046|ROM1_YEAST RHO1 GDP-GTP exchange protein 1 (Protein kinase C suppressor SKC1)
          Length = 1155

 Score = 57.4 bits (137), Expect = 6e-08
 Identities = 40/144 (27%), Positives = 75/144 (52%), Gaps = 1/144 (0%)
 Frame = +3

Query: 18  KGLAKHMKKEEIKYQESQFELISSEASYNRSLSVLVDTFYNSPQLSLTETEYFKPIIRPY 197
           K + + + K++IK QE+ +EL ++E  + +SL ++ DTF       L ET      +R  
Sbjct: 452 KSVWESLSKQQIKRQEAIYELFTTEKKFVKSLEIIRDTFMK----KLLETNIIPSDVRIN 507

Query: 198 QKEHLFSNILKIYAVSSSFLADLEHRFCESAKIIDLSDIVYKYSIKEFDVYVPYIQNQRF 377
             +H+F++I +IY+V+  FL  L  R   S     ++DI  +Y +  FD ++ YI ++ +
Sbjct: 508 FVKHVFAHINEIYSVNREFLKALAQRQSLSPICPGIADIFLQY-LPFFDPFLSYIASRPY 566

Query: 378 QSEAY-AELKKDPNTAKVFSELES 446
                  +   +PN A+   E+ +
Sbjct: 567 AKYLIETQRSVNPNFARFDDEVSN 590
>sp|Q9H8V3|ECT2_HUMAN ECT2 protein (Epithelial cell transforming sequence 2 oncogene)
          Length = 883

 Score = 48.5 bits (114), Expect = 3e-05
 Identities = 48/229 (20%), Positives = 99/229 (43%), Gaps = 2/229 (0%)
 Frame = +3

Query: 15   SKGLAKHMKKEEIKYQESQFELISSEASYNRSLSVLVDTFYNSPQLSLTET-EYFKPIIR 191
            SK       K+  ++Q ++ EL  +E++Y   L+ ++  F    Q+ L E  +   PI+ 
Sbjct: 409  SKSSTPVPSKQSARWQVAK-ELYQTESNYVNILATIIQLF----QVPLEEEGQRGGPILA 463

Query: 192  PYQKEHLFSNILKIYAVSSSFLADLEHRFCESAKIIDLSDIVYKYSIKEFDVYVPYIQNQ 371
            P + + +F +I  I+ V +    DLE       +   + DI  KYS      Y P++   
Sbjct: 464  PEEIKTIFGSIPDIFDVHTKIKDDLEDLIVNWDESKSIGDIFLKYSKDLVKTYPPFVNFF 523

Query: 372  RFQSEAYAELKKDPNTAKVFSEL-ESQPECCFLNLDSFXXXXXXXXXXXXXXXKAMKGNI 548
                E   + +K       F ++ +++PEC   +L                    +K + 
Sbjct: 524  EMSKETIIKCEKQKPRFHAFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKH- 582

Query: 549  QTNQKHICESIEKAISSMDKIIQKCSENKCHIDERKRMMEIYSCLDFKP 695
              ++     ++EKAI S+ +++   +E+K   + +K++ ++   +D  P
Sbjct: 583  TADENPDKSTLEKAIGSLKEVMTHINEDKRKTEAQKQIFDVVYEVDGCP 631
>sp|Q92888|ARHG1_HUMAN Rho guanine nucleotide exchange factor 1 (p115-RhoGEF) (p115RhoGEF)
            (115 kDa guanine nucleotide exchange factor) (Sub1.5)
          Length = 912

 Score = 47.4 bits (111), Expect = 6e-05
 Identities = 64/289 (22%), Positives = 121/289 (41%), Gaps = 23/289 (7%)
 Frame = +3

Query: 36   MKKEEIKYQESQFELISSEASYNRSLSVLVDTFYNSPQLSLTETEYFKPIIRPYQK-EHL 212
            + K ++K QE   EL+ +EA++ R L VL D F+      + E  +F     P ++ +++
Sbjct: 410  LPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFFQP----MAECLFF-----PLEELQNI 460

Query: 213  FSNILKIYAVSSSFLADLEHRFCESAKIID-LSDIVYKYSIKEFD---------VYVPYI 362
            F ++ ++  V S FL  L  R  ES  +I+ + D++    +  FD         +   + 
Sbjct: 461  FPSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVL----LARFDGAEGSWFQKISSRFC 516

Query: 363  QNQRFQSEAY-AELKKDPNTAKVFSELESQPECCFLNLDSFXXXXXXXXXXXXXXXKAMK 539
              Q F  E   A+ +KDP       E ES+P C  L L                  +++ 
Sbjct: 517  SRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIG 576

Query: 540  GNIQTNQKHICESIEKAISSMDKIIQKCSENKCHIDERKRMMEIYSCLDFKPGQNIREPL 719
             N  T +    E +E A     +I+   ++    +++  R+ +    LD    +   +P+
Sbjct: 577  QN--TEEPTEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLSHLRQSSDPM 634

Query: 720  -----------GKLIHEGEVRRTSKNSTKRQFKDYWFILTSEFLLMTKK 833
                        KL+HEG +   +   TK +  +   +L  + LL+ ++
Sbjct: 635  LSEFKNLDITKKKLVHEGPL---TWRVTKDKAVEVHVLLLDDLLLLLQR 680
>sp|Q07139|ECT2_MOUSE ECT2 protein (Epithelial cell transforming sequence 2 oncogene)
          Length = 738

 Score = 46.2 bits (108), Expect = 1e-04
 Identities = 45/220 (20%), Positives = 96/220 (43%), Gaps = 2/220 (0%)
 Frame = +3

Query: 42  KEEIKYQESQFELISSEASYNRSLSVLVDTFYNSPQLSLTET-EYFKPIIRPYQKEHLFS 218
           K+  ++Q ++ EL  +E++Y   L+ ++  F    Q+ L E  +   PI+ P + + +F 
Sbjct: 274 KQSARWQVAK-ELYQTESNYVNILATIIQLF----QVPLEEEGQRGGPILAPEEIKTIFG 328

Query: 219 NILKIYAVSSSFLADLEHRFCESAKIIDLSDIVYKYSIKEFDVYVPYIQNQRFQSEAYAE 398
           +I  I+ V      DLE       +   + DI  KY+      Y P++       E   +
Sbjct: 329 SIPDIFDVHMKIKDDLEDLIANWDESRSIGDIFLKYAKDLVKTYPPFVNFFEMSKEMIIK 388

Query: 399 LKKDPNTAKVFSEL-ESQPECCFLNLDSFXXXXXXXXXXXXXXXKAMKGNIQTNQKHICE 575
            +K       F ++ +++PEC   +L                    +K +   ++     
Sbjct: 389 CEKQKPRFHAFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKH-TADENPDKS 447

Query: 576 SIEKAISSMDKIIQKCSENKCHIDERKRMMEIYSCLDFKP 695
           ++EKAI S+ +++   +E+K   + +K++ ++   +D  P
Sbjct: 448 TLEKAIGSLKEVMTHINEDKRKTEAQKQIFDVVYEVDGCP 487
>sp|Q9Z1I6|ARHG1_RAT Rho guanine nucleotide exchange factor 1 (Lbc's second cousin)
          Length = 919

 Score = 45.4 bits (106), Expect = 2e-04
 Identities = 63/292 (21%), Positives = 122/292 (41%), Gaps = 23/292 (7%)
 Frame = +3

Query: 36   MKKEEIKYQESQFELISSEASYNRSLSVLVDTFYNSPQLSLTETEYFKPIIRPYQK-EHL 212
            + K ++K QE   EL+ +EA++ R L VL D FY      + E  +F     P ++ +++
Sbjct: 408  LPKNQVKRQEVISELLVTEAAHVRMLRVLHDLFYQP----MAEGGFF-----PLEELQNI 458

Query: 213  FSNILKIYAVSSSFLADLEHRFCESAKIID-LSDIVYKYSIKEFD---------VYVPYI 362
            F ++ ++  V S FL  L  R  ES  +I+ + D++    +  FD         +   + 
Sbjct: 459  FPSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVL----LARFDGAEGSWFQKISSRFC 514

Query: 363  QNQRFQSEAY-AELKKDPNTAKVFSELESQPECCFLNLDSFXXXXXXXXXXXXXXXKAMK 539
              Q F  E   A+ +K+P       E ES+P C  L L                  +++ 
Sbjct: 515  SRQSFALEQLKAKQRKEPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIG 574

Query: 540  GNIQTNQKHICESIEKAISSMDKIIQKCSENKCHIDERKRMMEIYSCLDFKPGQNIREPL 719
             N  T +      +E A     +I+   ++    +++  R+ +    LD    +   +P+
Sbjct: 575  QN--TEEPAERAKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLTHLRQSNDPM 632

Query: 720  -----------GKLIHEGEVRRTSKNSTKRQFKDYWFILTSEFLLMTKKSKG 842
                        KL+HEG +  T + +  +  + +  +L    LL+ ++ +G
Sbjct: 633  LSEFKNLDITKKKLVHEGPL--TWRLTKDKAVEVHVLLLDDLLLLLQRQDEG 682
>sp|Q9ULL1|PKHG1_HUMAN Pleckstrin homology domain containing family G member 1
          Length = 1385

 Score = 44.3 bits (103), Expect = 5e-04
 Identities = 56/253 (22%), Positives = 104/253 (41%)
 Frame = +3

Query: 75  ELISSEASYNRSLSVLVDTFYNSPQLSLTETEYFKPIIRPYQKEHLFSNILKIYAVSSSF 254
           E++ +E +Y + L  +V+ + +  +      +  K  +   ++  LF NI  IY  +S  
Sbjct: 120 EILETERTYVQDLKSIVEDYLDCIR------DQTKLPLGTEERSALFGNIQDIYHFNSEL 173

Query: 255 LADLEHRFCESAKIIDLSDIVYKYSIKEFDVYVPYIQNQRFQSEAYAELKKDPNTAKVFS 434
           L DLE+  CE+  +      V K   +EF +Y  Y  N         E  ++   AK F 
Sbjct: 174 LQDLEN--CENDPVAIAECFVSK--SEEFHIYTQYCTNYPRSVAVLTECMRNKILAKFFR 229

Query: 435 ELESQPECCFLNLDSFXXXXXXXXXXXXXXXKAMKGNIQTNQKHICESIEKAISSMDKII 614
           E +   +   L L S+                 ++ ++  + +   + +  AI +M ++ 
Sbjct: 230 ERQETLKHS-LPLGSYLLKPVQRILKYHLLLHEIENHLDKDTEGY-DVVLDAIDTMQRVA 287

Query: 615 QKCSENKCHIDERKRMMEIYSCLDFKPGQNIREPLGKLIHEGEVRRTSKNSTKRQFKDYW 794
              ++ K   +   R+ EI S L    G ++    G+L+ EG  R       +R   +  
Sbjct: 288 WHINDMKRKHEHAVRLQEIQSLLTNWKGPDLTS-YGELVLEGTFR------IQRAKNERT 340

Query: 795 FILTSEFLLMTKK 833
             L  + LL+TKK
Sbjct: 341 LFLFDKLLLITKK 353
>sp|Q61210|ARHG1_MOUSE Rho guanine nucleotide exchange factor 1 (Lymphoid blast crisis-like
            2) (Lbc's second cousin)
          Length = 920

 Score = 43.5 bits (101), Expect = 8e-04
 Identities = 61/288 (21%), Positives = 121/288 (42%), Gaps = 22/288 (7%)
 Frame = +3

Query: 36   MKKEEIKYQESQFELISSEASYNRSLSVLVDTFYNSPQLSLTETEYFKPIIRPYQKEHLF 215
            + K ++K QE   EL+ +EA++ R L VL D FY      + +  +F P+    + +++F
Sbjct: 409  LPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQP----MADGGFF-PLD---ELQNIF 460

Query: 216  SNILKIYAVSSSFLADLEHRFCESAKIID-LSDIVYKYSIKEFD---------VYVPYIQ 365
             ++ ++  V S FL  L  R  ES  +I+ + D++    +  FD         +   +  
Sbjct: 461  PSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVL----LARFDGAEGSWFQKISSRFCS 516

Query: 366  NQRFQSEAY-AELKKDPNTAKVFSELESQPECCFLNLDSFXXXXXXXXXXXXXXXKAMKG 542
             Q F  E   A+ +K+P       E ES+P C  L L                  +++  
Sbjct: 517  RQSFALEQLKAKQRKEPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQ 576

Query: 543  NIQTNQKHICESIEKAISSMDKIIQKCSENKCHIDERKRMMEIYSCLDFKPGQNIREPL- 719
            N + + +     +E A     +I+   ++    +++  R+ +    LD    +   +P+ 
Sbjct: 577  NTEESTER--GKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLTHLRQSSDPML 634

Query: 720  ----------GKLIHEGEVRRTSKNSTKRQFKDYWFILTSEFLLMTKK 833
                       KL+HEG +   +   TK +  +   +L  + LL+ ++
Sbjct: 635  SEFKNLDITKKKLVHEGPL---TWRVTKDKAIEVHVLLLDDLLLLLQR 679
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 96,861,142
Number of Sequences: 369166
Number of extensions: 1845698
Number of successful extensions: 5876
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5604
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5867
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9414689480
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)