Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_G18
(921 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O94989|ARHGF_HUMAN Rho guanine nucleotide exchange facto... 111 2e-24
sp|Q12774|ARHG5_HUMAN Rho guanine nucleotide exchange facto... 91 5e-18
sp|P51862|ROM2_YEAST RHO1 GDP-GTP exchange protein 2 59 1e-08
sp|P53046|ROM1_YEAST RHO1 GDP-GTP exchange protein 1 (Prote... 57 6e-08
sp|Q9H8V3|ECT2_HUMAN ECT2 protein (Epithelial cell transfor... 49 3e-05
sp|Q92888|ARHG1_HUMAN Rho guanine nucleotide exchange facto... 47 6e-05
sp|Q07139|ECT2_MOUSE ECT2 protein (Epithelial cell transfor... 46 1e-04
sp|Q9Z1I6|ARHG1_RAT Rho guanine nucleotide exchange factor ... 45 2e-04
sp|Q9ULL1|PKHG1_HUMAN Pleckstrin homology domain containing... 44 5e-04
sp|Q61210|ARHG1_MOUSE Rho guanine nucleotide exchange facto... 44 8e-04
>sp|O94989|ARHGF_HUMAN Rho guanine nucleotide exchange factor 15 (Vsm-RhoGEF)
Length = 841
Score = 111 bits (278), Expect = 2e-24
Identities = 86/291 (29%), Positives = 140/291 (48%), Gaps = 10/291 (3%)
Frame = +3
Query: 9 VRSKGLAKHMKKEEIKYQESQFELISSEASYNRSLSVLVDTFYNSPQLSLTETEYFKPII 188
V++ GL + +E + QES FE+++SEASY RSL +L DTF S L T T
Sbjct: 402 VQASGLLDTLSPQERRMQESLFEVVTSEASYLRSLRLLTDTFVLSQALRDTLT------- 454
Query: 189 RPYQKEHLFSNILKIYAVSSSFLADLEHRFCESAKIIDLSDIVYKYSIKEFDVYVPYIQN 368
P LFSN+ ++ VS FLA L R S I DL D+V+ +++ F VYV Y++N
Sbjct: 455 -PRDHHTLFSNVQRVQGVSERFLATLLSRVRSSPHISDLCDVVHAHAVGPFSVYVDYVRN 513
Query: 369 QRFQSEAYAELKKDPNT--AKVFSELESQPECCFLNLDSFXXXXXXXXXXXXXXXKAMKG 542
Q++Q E Y+ L D N + L+S P+C L L SF + +
Sbjct: 514 QQYQEETYSRL-MDTNVRFSAELRRLQSLPKCERLPLPSFLLLPFQRITRLRMLLQNILR 572
Query: 543 NIQTNQKHICESIEKAISSMDKIIQKCSENKCHIDERKRMMEIYSCLDFKPGQNI----- 707
+ E+ +KA+ ++ KII++CS + + + ++ + L F + +
Sbjct: 573 QTEEGSSR-QENAQKALGAVSKIIERCSAEVGRMKQTEELIRLTQRLRFHKVKALPLVSW 631
Query: 708 ---REPLGKLIHEGEVRRTSKNSTKRQFKDYWFILTSEFLLMTKKSKGKEM 851
E G+L G R +++ +F +L S+ LL+T+ G+ +
Sbjct: 632 SRRLEFQGELTELGCRRGGVLFASRPRFTPLCLLLFSDLLLITQPKSGQRL 682
>sp|Q12774|ARHG5_HUMAN Rho guanine nucleotide exchange factor 5 (Guanine nucleotide
regulatory protein TIM) (Oncogene TIM) (p60 TIM)
(Transforming immortalized mammary oncogene)
Length = 519
Score = 90.9 bits (224), Expect = 5e-18
Identities = 65/229 (28%), Positives = 110/229 (48%), Gaps = 1/229 (0%)
Frame = +3
Query: 9 VRSKGLAKHMKKEEIKYQESQFELISSEASYNRSLSVLVDTFYNSPQLSLTETEYFKPII 188
VR+ + M E+ K QE +FELI SEASY RSL++ VD F S L T +
Sbjct: 81 VRNSTVLLSMTHEDQKLQEVKFELIVSEASYLRSLNIAVDHFQLSTSLRAT--------L 132
Query: 189 RPYQKEHLFSNILKIYAVSSSFLADLEHRFCESAKIIDLSDIVYKYSIKEFDVYVPYIQN 368
+ + LFS + + VS++FL+DLE F + + D+V ++ VY+PY+ N
Sbjct: 133 SNQEHQWLFSRLQDVRDVSATFLSDLEENFENNIFSFQVCDVVLNHAPDFRRVYLPYVTN 192
Query: 369 QRFQSEAYAEL-KKDPNTAKVFSELESQPECCFLNLDSFXXXXXXXXXXXXXXXKAMKGN 545
Q +Q + L + N +V +LES P C L+L SF + +
Sbjct: 193 QTYQERTFQSLMNSNSNFREVLEKLESDPVCQRLSLKSFLILPFQRITRLKLLLQNILKR 252
Query: 546 IQTNQKHICESIEKAISSMDKIIQKCSENKCHIDERKRMMEIYSCLDFK 692
Q E+ KA +++++I+ C+ N + + ++ + ++F+
Sbjct: 253 TQPGSSEEAEA-TKAHHALEQLIRDCNNNVQSMRRTEELIYLSQKIEFE 300
>sp|P51862|ROM2_YEAST RHO1 GDP-GTP exchange protein 2
Length = 1356
Score = 59.3 bits (142), Expect = 1e-08
Identities = 63/272 (23%), Positives = 116/272 (42%), Gaps = 5/272 (1%)
Frame = +3
Query: 30 KHMKKEEIKYQESQFELISSEASYNRSLSVLVDTFYNSPQLSLTETEYFKPIIRPYQKEH 209
+++ K+EIK QE+ +E+ +E ++ +SL + DTF + L ET IR +H
Sbjct: 651 ENLPKKEIKRQEAIYEVYITEKNFVKSLEITRDTFMKT----LAETNIISADIRKNFIKH 706
Query: 210 LFSNILKIYAVSSSFLADLEHRFCESAKIIDLSDIVYKYSIKEFDVYVPYIQNQRFQSEA 389
+F++I IY+V+ FL L R S + + DIV ++ I F+ +V Y+ ++ + ++
Sbjct: 707 VFAHINDIYSVNRRFLKALTDRQRSSPVVRGIGDIVLRF-IPFFEPFVSYVASRPY-AKY 764
Query: 390 YAELKKDPNTAKVFSELESQPECCFLNLDSFXXXXXXXXXXXXXXXK-AMKGNIQTNQKH 566
E ++ N + + +DSF K MK K
Sbjct: 765 LIETQRSVNPYFARFDDDMMSSSLRHGIDSFLSQGVSRPGRYMLLVKEIMKSTDPEKDKS 824
Query: 567 ICESIEKAISSMDKIIQKCSENKCHIDERKRMMEIYSCLDFK---PGQNIREPLGKLIHE 737
E + KA+ ++ +++ + +R + + + FK + + K+ HE
Sbjct: 825 DYEDLSKAMDALRDFMKRIDQASGAAQDRHDVKLLKQKILFKNEYVNLGLNDERRKIKHE 884
Query: 738 GEV-RRTSKNSTKRQFKDYWFILTSEFLLMTK 830
G + R+ S D F L LL K
Sbjct: 885 GILSRKELSKSDGTVVGDIQFYLLDNMLLFLK 916
>sp|P53046|ROM1_YEAST RHO1 GDP-GTP exchange protein 1 (Protein kinase C suppressor SKC1)
Length = 1155
Score = 57.4 bits (137), Expect = 6e-08
Identities = 40/144 (27%), Positives = 75/144 (52%), Gaps = 1/144 (0%)
Frame = +3
Query: 18 KGLAKHMKKEEIKYQESQFELISSEASYNRSLSVLVDTFYNSPQLSLTETEYFKPIIRPY 197
K + + + K++IK QE+ +EL ++E + +SL ++ DTF L ET +R
Sbjct: 452 KSVWESLSKQQIKRQEAIYELFTTEKKFVKSLEIIRDTFMK----KLLETNIIPSDVRIN 507
Query: 198 QKEHLFSNILKIYAVSSSFLADLEHRFCESAKIIDLSDIVYKYSIKEFDVYVPYIQNQRF 377
+H+F++I +IY+V+ FL L R S ++DI +Y + FD ++ YI ++ +
Sbjct: 508 FVKHVFAHINEIYSVNREFLKALAQRQSLSPICPGIADIFLQY-LPFFDPFLSYIASRPY 566
Query: 378 QSEAY-AELKKDPNTAKVFSELES 446
+ +PN A+ E+ +
Sbjct: 567 AKYLIETQRSVNPNFARFDDEVSN 590
>sp|Q9H8V3|ECT2_HUMAN ECT2 protein (Epithelial cell transforming sequence 2 oncogene)
Length = 883
Score = 48.5 bits (114), Expect = 3e-05
Identities = 48/229 (20%), Positives = 99/229 (43%), Gaps = 2/229 (0%)
Frame = +3
Query: 15 SKGLAKHMKKEEIKYQESQFELISSEASYNRSLSVLVDTFYNSPQLSLTET-EYFKPIIR 191
SK K+ ++Q ++ EL +E++Y L+ ++ F Q+ L E + PI+
Sbjct: 409 SKSSTPVPSKQSARWQVAK-ELYQTESNYVNILATIIQLF----QVPLEEEGQRGGPILA 463
Query: 192 PYQKEHLFSNILKIYAVSSSFLADLEHRFCESAKIIDLSDIVYKYSIKEFDVYVPYIQNQ 371
P + + +F +I I+ V + DLE + + DI KYS Y P++
Sbjct: 464 PEEIKTIFGSIPDIFDVHTKIKDDLEDLIVNWDESKSIGDIFLKYSKDLVKTYPPFVNFF 523
Query: 372 RFQSEAYAELKKDPNTAKVFSEL-ESQPECCFLNLDSFXXXXXXXXXXXXXXXKAMKGNI 548
E + +K F ++ +++PEC +L +K +
Sbjct: 524 EMSKETIIKCEKQKPRFHAFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKH- 582
Query: 549 QTNQKHICESIEKAISSMDKIIQKCSENKCHIDERKRMMEIYSCLDFKP 695
++ ++EKAI S+ +++ +E+K + +K++ ++ +D P
Sbjct: 583 TADENPDKSTLEKAIGSLKEVMTHINEDKRKTEAQKQIFDVVYEVDGCP 631
>sp|Q92888|ARHG1_HUMAN Rho guanine nucleotide exchange factor 1 (p115-RhoGEF) (p115RhoGEF)
(115 kDa guanine nucleotide exchange factor) (Sub1.5)
Length = 912
Score = 47.4 bits (111), Expect = 6e-05
Identities = 64/289 (22%), Positives = 121/289 (41%), Gaps = 23/289 (7%)
Frame = +3
Query: 36 MKKEEIKYQESQFELISSEASYNRSLSVLVDTFYNSPQLSLTETEYFKPIIRPYQK-EHL 212
+ K ++K QE EL+ +EA++ R L VL D F+ + E +F P ++ +++
Sbjct: 410 LPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFFQP----MAECLFF-----PLEELQNI 460
Query: 213 FSNILKIYAVSSSFLADLEHRFCESAKIID-LSDIVYKYSIKEFD---------VYVPYI 362
F ++ ++ V S FL L R ES +I+ + D++ + FD + +
Sbjct: 461 FPSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVL----LARFDGAEGSWFQKISSRFC 516
Query: 363 QNQRFQSEAY-AELKKDPNTAKVFSELESQPECCFLNLDSFXXXXXXXXXXXXXXXKAMK 539
Q F E A+ +KDP E ES+P C L L +++
Sbjct: 517 SRQSFALEQLKAKQRKDPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIG 576
Query: 540 GNIQTNQKHICESIEKAISSMDKIIQKCSENKCHIDERKRMMEIYSCLDFKPGQNIREPL 719
N T + E +E A +I+ ++ +++ R+ + LD + +P+
Sbjct: 577 QN--TEEPTEREKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLSHLRQSSDPM 634
Query: 720 -----------GKLIHEGEVRRTSKNSTKRQFKDYWFILTSEFLLMTKK 833
KL+HEG + + TK + + +L + LL+ ++
Sbjct: 635 LSEFKNLDITKKKLVHEGPL---TWRVTKDKAVEVHVLLLDDLLLLLQR 680
>sp|Q07139|ECT2_MOUSE ECT2 protein (Epithelial cell transforming sequence 2 oncogene)
Length = 738
Score = 46.2 bits (108), Expect = 1e-04
Identities = 45/220 (20%), Positives = 96/220 (43%), Gaps = 2/220 (0%)
Frame = +3
Query: 42 KEEIKYQESQFELISSEASYNRSLSVLVDTFYNSPQLSLTET-EYFKPIIRPYQKEHLFS 218
K+ ++Q ++ EL +E++Y L+ ++ F Q+ L E + PI+ P + + +F
Sbjct: 274 KQSARWQVAK-ELYQTESNYVNILATIIQLF----QVPLEEEGQRGGPILAPEEIKTIFG 328
Query: 219 NILKIYAVSSSFLADLEHRFCESAKIIDLSDIVYKYSIKEFDVYVPYIQNQRFQSEAYAE 398
+I I+ V DLE + + DI KY+ Y P++ E +
Sbjct: 329 SIPDIFDVHMKIKDDLEDLIANWDESRSIGDIFLKYAKDLVKTYPPFVNFFEMSKEMIIK 388
Query: 399 LKKDPNTAKVFSEL-ESQPECCFLNLDSFXXXXXXXXXXXXXXXKAMKGNIQTNQKHICE 575
+K F ++ +++PEC +L +K + ++
Sbjct: 389 CEKQKPRFHAFLKINQAKPECGRQSLVELLIRPVQRLPSVALLLNDLKKH-TADENPDKS 447
Query: 576 SIEKAISSMDKIIQKCSENKCHIDERKRMMEIYSCLDFKP 695
++EKAI S+ +++ +E+K + +K++ ++ +D P
Sbjct: 448 TLEKAIGSLKEVMTHINEDKRKTEAQKQIFDVVYEVDGCP 487
>sp|Q9Z1I6|ARHG1_RAT Rho guanine nucleotide exchange factor 1 (Lbc's second cousin)
Length = 919
Score = 45.4 bits (106), Expect = 2e-04
Identities = 63/292 (21%), Positives = 122/292 (41%), Gaps = 23/292 (7%)
Frame = +3
Query: 36 MKKEEIKYQESQFELISSEASYNRSLSVLVDTFYNSPQLSLTETEYFKPIIRPYQK-EHL 212
+ K ++K QE EL+ +EA++ R L VL D FY + E +F P ++ +++
Sbjct: 408 LPKNQVKRQEVISELLVTEAAHVRMLRVLHDLFYQP----MAEGGFF-----PLEELQNI 458
Query: 213 FSNILKIYAVSSSFLADLEHRFCESAKIID-LSDIVYKYSIKEFD---------VYVPYI 362
F ++ ++ V S FL L R ES +I+ + D++ + FD + +
Sbjct: 459 FPSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVL----LARFDGAEGSWFQKISSRFC 514
Query: 363 QNQRFQSEAY-AELKKDPNTAKVFSELESQPECCFLNLDSFXXXXXXXXXXXXXXXKAMK 539
Q F E A+ +K+P E ES+P C L L +++
Sbjct: 515 SRQSFALEQLKAKQRKEPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIG 574
Query: 540 GNIQTNQKHICESIEKAISSMDKIIQKCSENKCHIDERKRMMEIYSCLDFKPGQNIREPL 719
N T + +E A +I+ ++ +++ R+ + LD + +P+
Sbjct: 575 QN--TEEPAERAKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLTHLRQSNDPM 632
Query: 720 -----------GKLIHEGEVRRTSKNSTKRQFKDYWFILTSEFLLMTKKSKG 842
KL+HEG + T + + + + + +L LL+ ++ +G
Sbjct: 633 LSEFKNLDITKKKLVHEGPL--TWRLTKDKAVEVHVLLLDDLLLLLQRQDEG 682
>sp|Q9ULL1|PKHG1_HUMAN Pleckstrin homology domain containing family G member 1
Length = 1385
Score = 44.3 bits (103), Expect = 5e-04
Identities = 56/253 (22%), Positives = 104/253 (41%)
Frame = +3
Query: 75 ELISSEASYNRSLSVLVDTFYNSPQLSLTETEYFKPIIRPYQKEHLFSNILKIYAVSSSF 254
E++ +E +Y + L +V+ + + + + K + ++ LF NI IY +S
Sbjct: 120 EILETERTYVQDLKSIVEDYLDCIR------DQTKLPLGTEERSALFGNIQDIYHFNSEL 173
Query: 255 LADLEHRFCESAKIIDLSDIVYKYSIKEFDVYVPYIQNQRFQSEAYAELKKDPNTAKVFS 434
L DLE+ CE+ + V K +EF +Y Y N E ++ AK F
Sbjct: 174 LQDLEN--CENDPVAIAECFVSK--SEEFHIYTQYCTNYPRSVAVLTECMRNKILAKFFR 229
Query: 435 ELESQPECCFLNLDSFXXXXXXXXXXXXXXXKAMKGNIQTNQKHICESIEKAISSMDKII 614
E + + L L S+ ++ ++ + + + + AI +M ++
Sbjct: 230 ERQETLKHS-LPLGSYLLKPVQRILKYHLLLHEIENHLDKDTEGY-DVVLDAIDTMQRVA 287
Query: 615 QKCSENKCHIDERKRMMEIYSCLDFKPGQNIREPLGKLIHEGEVRRTSKNSTKRQFKDYW 794
++ K + R+ EI S L G ++ G+L+ EG R +R +
Sbjct: 288 WHINDMKRKHEHAVRLQEIQSLLTNWKGPDLTS-YGELVLEGTFR------IQRAKNERT 340
Query: 795 FILTSEFLLMTKK 833
L + LL+TKK
Sbjct: 341 LFLFDKLLLITKK 353
>sp|Q61210|ARHG1_MOUSE Rho guanine nucleotide exchange factor 1 (Lymphoid blast crisis-like
2) (Lbc's second cousin)
Length = 920
Score = 43.5 bits (101), Expect = 8e-04
Identities = 61/288 (21%), Positives = 121/288 (42%), Gaps = 22/288 (7%)
Frame = +3
Query: 36 MKKEEIKYQESQFELISSEASYNRSLSVLVDTFYNSPQLSLTETEYFKPIIRPYQKEHLF 215
+ K ++K QE EL+ +EA++ R L VL D FY + + +F P+ + +++F
Sbjct: 409 LPKSQVKRQEVISELLVTEAAHVRMLRVLHDLFYQP----MADGGFF-PLD---ELQNIF 460
Query: 216 SNILKIYAVSSSFLADLEHRFCESAKIID-LSDIVYKYSIKEFD---------VYVPYIQ 365
++ ++ V S FL L R ES +I+ + D++ + FD + +
Sbjct: 461 PSLDELIEVHSLFLDRLMKRRQESGYLIEEIGDVL----LARFDGAEGSWFQKISSRFCS 516
Query: 366 NQRFQSEAY-AELKKDPNTAKVFSELESQPECCFLNLDSFXXXXXXXXXXXXXXXKAMKG 542
Q F E A+ +K+P E ES+P C L L +++
Sbjct: 517 RQSFALEQLKAKQRKEPRFCAFVQEAESRPRCRRLQLKDMIPTEMQRLTKYPLLLQSIGQ 576
Query: 543 NIQTNQKHICESIEKAISSMDKIIQKCSENKCHIDERKRMMEIYSCLDFKPGQNIREPL- 719
N + + + +E A +I+ ++ +++ R+ + LD + +P+
Sbjct: 577 NTEESTER--GKVELAAECCREILHHVNQAVRDMEDLLRLKDYQRRLDLTHLRQSSDPML 634
Query: 720 ----------GKLIHEGEVRRTSKNSTKRQFKDYWFILTSEFLLMTKK 833
KL+HEG + + TK + + +L + LL+ ++
Sbjct: 635 SEFKNLDITKKKLVHEGPL---TWRVTKDKAIEVHVLLLDDLLLLLQR 679
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 96,861,142
Number of Sequences: 369166
Number of extensions: 1845698
Number of successful extensions: 5876
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5604
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5867
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 9414689480
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)