Planaria EST Database


DrC_01165

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01165
         (807 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9FZ36|M3K2_ARATH  Mitogen-activated protein kinase kinas...   117   5e-26
sp|O22040|M3K1_ARATH  Mitogen-activated protein kinase kinas...   116   8e-26
sp|O22042|M3K3_ARATH  Mitogen-activated protein kinase kinas...   115   2e-25
sp|Q99683|M3K5_HUMAN  Mitogen-activated protein kinase kinas...   109   8e-24
sp|P23561|STE11_YEAST  Serine/threonine-protein kinase STE11      102   1e-21
sp|O95382|M3K6_HUMAN  Mitogen-activated protein kinase kinas...   102   1e-21
sp|Q99759|M3K3_HUMAN  Mitogen-activated protein kinase kinas...   100   4e-21
sp|O08648|M3K4_MOUSE  Mitogen-activated protein kinase kinas...   100   5e-21
sp|Q9Y6R4|M3K4_HUMAN  Mitogen-activated protein kinase kinas...   100   5e-21
sp|Q61084|M3K3_MOUSE  Mitogen-activated protein kinase kinas...   100   6e-21
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
           NPK1-related protein kinase 2)
          Length = 651

 Score =  117 bits (292), Expect = 5e-26
 Identities = 72/196 (36%), Positives = 102/196 (52%), Gaps = 3/196 (1%)
 Frame = +3

Query: 207 GSISEKISLNNYQPLPVETIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI* 386
           GSIS    L  +   P   ++ YT Q+L GL++LH   I+HRDIK               
Sbjct: 156 GSISSL--LEKFGAFPESVVRTYTNQLLLGLEYLHNHAIMHRDIK--------------- 198

Query: 387 IGANILITKQDCIKIVDFGLSRFLQTVSRLE-KKTFCGTVHYTSPEVINDESYGFPADIW 563
            GANIL+  Q CIK+ DFG S+ +  ++ +   K+  GT ++ +PEVI    + F ADIW
Sbjct: 199 -GANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPYWMAPEVILQTGHSFSADIW 257

Query: 564 SVGATVFEMATGRPPYHSAKNEIAVAVKIATTKRLDYSKTLEDN--KETKQFIDLMTKAD 737
           SVG TV EM TG+ P+     EIA    I TTK       + DN   +   F+    + +
Sbjct: 258 SVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKS---HPPIPDNISSDANDFLLKCLQQE 314

Query: 738 PNKRKTADQLLNYHYL 785
           PN R TA +LL + ++
Sbjct: 315 PNLRPTASELLKHPFV 330
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
           NPK1-related protein kinase 1)
          Length = 666

 Score =  116 bits (290), Expect = 8e-26
 Identities = 72/195 (36%), Positives = 104/195 (53%), Gaps = 2/195 (1%)
 Frame = +3

Query: 207 GSISEKISLNNYQPLPVETIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI* 386
           GSIS    L  + P P   ++ YT+Q+L GL++LH   I+HRDIK               
Sbjct: 157 GSISSL--LEKFGPFPESVVRTYTRQLLLGLEYLHNHAIMHRDIK--------------- 199

Query: 387 IGANILITKQDCIKIVDFGLSRFLQTVSRLE-KKTFCGTVHYTSPEVINDESYGFPADIW 563
            GANIL+  + CIK+ DFG S+ +  ++ +   K+  GT ++ +PEVI    + F ADIW
Sbjct: 200 -GANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTPYWMAPEVILQTGHSFSADIW 258

Query: 564 SVGATVFEMATGRPPYHSAKNEIAVAVKIATTK-RLDYSKTLEDNKETKQFIDLMTKADP 740
           SVG TV EM TG+ P+     E+A    I TTK       TL  + + K F+    +  P
Sbjct: 259 SVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTL--SSDAKDFLLKCLQEVP 316

Query: 741 NKRKTADQLLNYHYL 785
           N R TA +LL + ++
Sbjct: 317 NLRPTASELLKHPFV 331
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
           NPK1-related protein kinase 3)
          Length = 651

 Score =  115 bits (287), Expect = 2e-25
 Identities = 71/195 (36%), Positives = 106/195 (54%), Gaps = 2/195 (1%)
 Frame = +3

Query: 207 GSISEKISLNNYQPLPVETIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI* 386
           GSIS    L  +   P   I +YTKQ+L GL++LH + I+HRDIK               
Sbjct: 156 GSISSL--LEKFGSFPEPVIIMYTKQLLLGLEYLHNNGIMHRDIK--------------- 198

Query: 387 IGANILITKQDCIKIVDFGLSRFLQTVSRLE-KKTFCGTVHYTSPEVINDESYGFPADIW 563
            GANIL+  + CI++ DFG S+ +  ++ +   K+  GT ++ +PEVI    + F ADIW
Sbjct: 199 -GANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPYWMAPEVILQTGHSFSADIW 257

Query: 564 SVGATVFEMATGRPPYHSAKNEIAVAVKIATTKRLDYSKTLED-NKETKQFIDLMTKADP 740
           SVG TV EMATG+PP+     + A  + I  TK   +    ED + E K F+      +P
Sbjct: 258 SVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKA--HPPIPEDLSPEAKDFLMKCLHKEP 315

Query: 741 NKRKTADQLLNYHYL 785
           + R +A +LL + ++
Sbjct: 316 SLRLSATELLQHPFV 330
>sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinase kinase 5 (MAPK/ERK kinase
            kinase 5) (MEK kinase 5) (MEKK 5) (Apoptosis
            signal-regulating kinase 1) (ASK-1)
          Length = 1374

 Score =  109 bits (273), Expect = 8e-24
 Identities = 71/181 (39%), Positives = 100/181 (55%), Gaps = 4/181 (2%)
 Frame = +3

Query: 258  ETIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI*IGANILI-TKQDCIKIV 434
            +TI  YTKQIL GLK+LH + I+HRDIK                G N+LI T    +KI 
Sbjct: 778  QTIGFYTKQILEGLKYLHDNQIVHRDIK----------------GDNVLINTYSGVLKIS 821

Query: 435  DFGLSRFLQTVSRLEKKTFCGTVHYTSPEVIN--DESYGFPADIWSVGATVFEMATGRPP 608
            DFG S+ L  ++    +TF GT+ Y +PE+I+     YG  ADIWS+G T+ EMATG+PP
Sbjct: 822  DFGTSKRLAGINPC-TETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPP 880

Query: 609  YHSAKNEIAVAVKIATTK-RLDYSKTLEDNKETKQFIDLMTKADPNKRKTADQLLNYHYL 785
            ++      A   K+   K   +  +++  + E K FI    + DP+KR  A+ LL   +L
Sbjct: 881  FYELGEPQAAMFKVGMFKVHPEIPESM--SAEAKAFILKCFEPDPDKRACANDLLVDEFL 938

Query: 786  K 788
            K
Sbjct: 939  K 939
>sp|P23561|STE11_YEAST Serine/threonine-protein kinase STE11
          Length = 738

 Score =  102 bits (254), Expect = 1e-21
 Identities = 69/195 (35%), Positives = 100/195 (51%), Gaps = 2/195 (1%)
 Frame = +3

Query: 207  GSISEKISLNNYQPLPVETIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI* 386
            GS+S    LNNY P     I  +T+QIL G+ +LH+ NIIHRDIK               
Sbjct: 560  GSVSSM--LNNYGPFEESLITNFTRQILIGVAYLHKKNIIHRDIK--------------- 602

Query: 387  IGANILITKQDCIKIVDFGLSRFLQTVSRLEKK--TFCGTVHYTSPEVINDESYGFPADI 560
             GANILI  + C+KI DFG+S+ L  +++ + K  +  G+V + SPEV+   +    ADI
Sbjct: 603  -GANILIDIKGCVKITDFGISKKLSPLNKKQNKRASLQGSVFWMSPEVVKQTATTAKADI 661

Query: 561  WSVGATVFEMATGRPPYHSAKNEIAVAVKIATTKRLDYSKTLEDNKETKQFIDLMTKADP 740
            WS G  V EM TG+ P+    +++    KI T    +         E K F+    + D 
Sbjct: 662  WSTGCVVIEMFTGKHPFPDF-SQMQAIFKIGTNTTPEIPSWA--TSEGKNFLRKAFELDY 718

Query: 741  NKRKTADQLLNYHYL 785
              R +A +LL + +L
Sbjct: 719  QYRPSALELLQHPWL 733
>sp|O95382|M3K6_HUMAN Mitogen-activated protein kinase kinase kinase 6
          Length = 1011

 Score =  102 bits (254), Expect = 1e-21
 Identities = 67/179 (37%), Positives = 95/179 (53%), Gaps = 3/179 (1%)
 Frame = +3

Query: 261 TIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI*IGANILI-TKQDCIKIVD 437
           TI  YT+QIL GL +LH ++I+HRDIK                G N+LI T    +KI D
Sbjct: 470 TISFYTRQILQGLGYLHDNHIVHRDIK----------------GDNVLINTFSGLLKISD 513

Query: 438 FGLSRFLQTVSRLEKKTFCGTVHYTSPEVIND--ESYGFPADIWSVGATVFEMATGRPPY 611
           FG S+ L  ++   + TF GT+ Y +PE+I+     YG  ADIWS+G TV EMATGRPP+
Sbjct: 514 FGTSKRLAGITPCTE-TFTGTLQYMAPEIIDQGPRGYGKAADIWSLGCTVIEMATGRPPF 572

Query: 612 HSAKNEIAVAVKIATTKRLDYSKTLEDNKETKQFIDLMTKADPNKRKTADQLLNYHYLK 788
           H   +  A   ++   K +        + E + F+    + DP  R +A  LL   +L+
Sbjct: 573 HELGSPQAAMFQVGMYK-VHPPMPSSLSAEAQAFLLRTFEPDPRLRASAQTLLGDPFLQ 630
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
           kinase 3) (MEK kinase 3) (MEKK 3)
          Length = 626

 Score =  100 bits (250), Expect = 4e-21
 Identities = 65/191 (34%), Positives = 100/191 (52%), Gaps = 2/191 (1%)
 Frame = +3

Query: 216 SEKISLNNYQPLPVETIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI*IGA 395
           S K  L  Y  L     + YT+QIL G+ +LH + I+HRDIK                GA
Sbjct: 450 SVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIK----------------GA 493

Query: 396 NILITKQDCIKIVDFGLSRFLQTV--SRLEKKTFCGTVHYTSPEVINDESYGFPADIWSV 569
           NIL      +K+ DFG S+ LQT+  S    ++  GT ++ SPEVI+ E YG  AD+WS+
Sbjct: 494 NILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVTGTPYWMSPEVISGEGYGRKADVWSL 553

Query: 570 GATVFEMATGRPPYHSAKNEIAVAVKIATTKRLDYSKTLEDNKETKQFIDLMTKADPNKR 749
           G TV EM T +PP+  A+ E   A+    T+  +       ++  + F+  +   +  +R
Sbjct: 554 GCTVVEMLTEKPPW--AEYEAMAAIFKIATQPTNPQLPSHISEHGRDFLRRIF-VEARQR 610

Query: 750 KTADQLLNYHY 782
            +A++LL +H+
Sbjct: 611 PSAEELLTHHF 621
>sp|O08648|M3K4_MOUSE Mitogen-activated protein kinase kinase kinase 4 (MAPK/ERK kinase
            kinase 4) (MEK kinase 4) (MEKK 4)
          Length = 1597

 Score =  100 bits (249), Expect = 5e-21
 Identities = 65/186 (34%), Positives = 94/186 (50%), Gaps = 6/186 (3%)
 Frame = +3

Query: 249  LPVETIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI*IGANILITKQDCIK 428
            L    I+LYTKQI   +  LH+  I+HRDIK                GANI +T    IK
Sbjct: 1424 LQEHVIRLYTKQITVAINVLHEHGIVHRDIK----------------GANIFLTSSGLIK 1467

Query: 429  IVDFGLSRFLQTVSRL---EKKTFCGTVHYTSPEVIN---DESYGFPADIWSVGATVFEM 590
            + DFG S  L+  ++    E  +  GT  Y +PEVI     E +G  ADIWS+G  V EM
Sbjct: 1468 LGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEM 1527

Query: 591  ATGRPPYHSAKNEIAVAVKIATTKRLDYSKTLEDNKETKQFIDLMTKADPNKRKTADQLL 770
             TG+ P+H  ++   +  K+    +    + L  + E K F+    ++DP  R TA QLL
Sbjct: 1528 VTGKRPWHEYEHNFQIMYKVGMGHKPPIPERL--SPEGKAFLSHCLESDPKIRWTASQLL 1585

Query: 771  NYHYLK 788
            ++ ++K
Sbjct: 1586 DHAFVK 1591
>sp|Q9Y6R4|M3K4_HUMAN Mitogen-activated protein kinase kinase kinase 4 (MAPK/ERK kinase
            kinase 4) (MEK kinase 4) (MEKK 4) (MAP three kinase 1)
          Length = 1607

 Score =  100 bits (249), Expect = 5e-21
 Identities = 64/186 (34%), Positives = 94/186 (50%), Gaps = 6/186 (3%)
 Frame = +3

Query: 249  LPVETIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI*IGANILITKQDCIK 428
            L    I+LY+KQI   +  LH+  I+HRDIK                GANI +T    IK
Sbjct: 1434 LQEHVIRLYSKQITIAINVLHEHGIVHRDIK----------------GANIFLTSSGLIK 1477

Query: 429  IVDFGLSRFLQTVSRL---EKKTFCGTVHYTSPEVIN---DESYGFPADIWSVGATVFEM 590
            + DFG S  L+  ++    E  +  GT  Y +PEVI     E +G  ADIWS+G  V EM
Sbjct: 1478 LGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEM 1537

Query: 591  ATGRPPYHSAKNEIAVAVKIATTKRLDYSKTLEDNKETKQFIDLMTKADPNKRKTADQLL 770
             TG+ P+H  ++   +  K+    +    + L  + E K F+    ++DP  R TA QLL
Sbjct: 1538 VTGKRPWHEYEHNFQIMYKVGMGHKPPIPERL--SPEGKDFLSHCLESDPKMRWTASQLL 1595

Query: 771  NYHYLK 788
            ++ ++K
Sbjct: 1596 DHSFVK 1601
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
           kinase 3) (MEK kinase 3) (MEKK 3)
          Length = 626

 Score =  100 bits (248), Expect = 6e-21
 Identities = 65/191 (34%), Positives = 100/191 (52%), Gaps = 2/191 (1%)
 Frame = +3

Query: 216 SEKISLNNYQPLPVETIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI*IGA 395
           S K  L  Y  L     + YT+QIL G+ +LH + I+HRDIK                GA
Sbjct: 450 SVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIK----------------GA 493

Query: 396 NILITKQDCIKIVDFGLSRFLQTV--SRLEKKTFCGTVHYTSPEVINDESYGFPADIWSV 569
           NIL      +K+ DFG S+ LQT+  S    ++  GT ++ SPEVI+ E YG  AD+WS+
Sbjct: 494 NILRDSAGNVKLGDFGASKRLQTICMSGTGIRSVTGTPYWMSPEVISGEGYGRKADVWSL 553

Query: 570 GATVFEMATGRPPYHSAKNEIAVAVKIATTKRLDYSKTLEDNKETKQFIDLMTKADPNKR 749
           G TV EM T +PP+  A+ E   A+    T+  +       ++  + F+  +   +  +R
Sbjct: 554 GCTVVEMLTEKPPW--AEYEAMAAIFKIATQPTNPQLPSHISEHGRDFLRRIF-VEARQR 610

Query: 750 KTADQLLNYHY 782
            +A++LL +H+
Sbjct: 611 PSAEELLTHHF 621
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 73,949,670
Number of Sequences: 369166
Number of extensions: 1323346
Number of successful extensions: 7395
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4849
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5076
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7666799535
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)