Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_001_E02
(807 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 117 5e-26
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 116 8e-26
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 115 2e-25
sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinas... 109 8e-24
sp|P23561|STE11_YEAST Serine/threonine-protein kinase STE11 102 1e-21
sp|O95382|M3K6_HUMAN Mitogen-activated protein kinase kinas... 102 1e-21
sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinas... 100 4e-21
sp|O08648|M3K4_MOUSE Mitogen-activated protein kinase kinas... 100 5e-21
sp|Q9Y6R4|M3K4_HUMAN Mitogen-activated protein kinase kinas... 100 5e-21
sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinas... 100 6e-21
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 117 bits (292), Expect = 5e-26
Identities = 72/196 (36%), Positives = 102/196 (52%), Gaps = 3/196 (1%)
Frame = +3
Query: 207 GSISEKISLNNYQPLPVETIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI* 386
GSIS L + P ++ YT Q+L GL++LH I+HRDIK
Sbjct: 156 GSISSL--LEKFGAFPESVVRTYTNQLLLGLEYLHNHAIMHRDIK--------------- 198
Query: 387 IGANILITKQDCIKIVDFGLSRFLQTVSRLE-KKTFCGTVHYTSPEVINDESYGFPADIW 563
GANIL+ Q CIK+ DFG S+ + ++ + K+ GT ++ +PEVI + F ADIW
Sbjct: 199 -GANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPYWMAPEVILQTGHSFSADIW 257
Query: 564 SVGATVFEMATGRPPYHSAKNEIAVAVKIATTKRLDYSKTLEDN--KETKQFIDLMTKAD 737
SVG TV EM TG+ P+ EIA I TTK + DN + F+ + +
Sbjct: 258 SVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKS---HPPIPDNISSDANDFLLKCLQQE 314
Query: 738 PNKRKTADQLLNYHYL 785
PN R TA +LL + ++
Sbjct: 315 PNLRPTASELLKHPFV 330
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 116 bits (290), Expect = 8e-26
Identities = 72/195 (36%), Positives = 104/195 (53%), Gaps = 2/195 (1%)
Frame = +3
Query: 207 GSISEKISLNNYQPLPVETIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI* 386
GSIS L + P P ++ YT+Q+L GL++LH I+HRDIK
Sbjct: 157 GSISSL--LEKFGPFPESVVRTYTRQLLLGLEYLHNHAIMHRDIK--------------- 199
Query: 387 IGANILITKQDCIKIVDFGLSRFLQTVSRLE-KKTFCGTVHYTSPEVINDESYGFPADIW 563
GANIL+ + CIK+ DFG S+ + ++ + K+ GT ++ +PEVI + F ADIW
Sbjct: 200 -GANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTPYWMAPEVILQTGHSFSADIW 258
Query: 564 SVGATVFEMATGRPPYHSAKNEIAVAVKIATTK-RLDYSKTLEDNKETKQFIDLMTKADP 740
SVG TV EM TG+ P+ E+A I TTK TL + + K F+ + P
Sbjct: 259 SVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIPDTL--SSDAKDFLLKCLQEVP 316
Query: 741 NKRKTADQLLNYHYL 785
N R TA +LL + ++
Sbjct: 317 NLRPTASELLKHPFV 331
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 115 bits (287), Expect = 2e-25
Identities = 71/195 (36%), Positives = 106/195 (54%), Gaps = 2/195 (1%)
Frame = +3
Query: 207 GSISEKISLNNYQPLPVETIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI* 386
GSIS L + P I +YTKQ+L GL++LH + I+HRDIK
Sbjct: 156 GSISSL--LEKFGSFPEPVIIMYTKQLLLGLEYLHNNGIMHRDIK--------------- 198
Query: 387 IGANILITKQDCIKIVDFGLSRFLQTVSRLE-KKTFCGTVHYTSPEVINDESYGFPADIW 563
GANIL+ + CI++ DFG S+ + ++ + K+ GT ++ +PEVI + F ADIW
Sbjct: 199 -GANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPYWMAPEVILQTGHSFSADIW 257
Query: 564 SVGATVFEMATGRPPYHSAKNEIAVAVKIATTKRLDYSKTLED-NKETKQFIDLMTKADP 740
SVG TV EMATG+PP+ + A + I TK + ED + E K F+ +P
Sbjct: 258 SVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKA--HPPIPEDLSPEAKDFLMKCLHKEP 315
Query: 741 NKRKTADQLLNYHYL 785
+ R +A +LL + ++
Sbjct: 316 SLRLSATELLQHPFV 330
>sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinase kinase 5 (MAPK/ERK kinase
kinase 5) (MEK kinase 5) (MEKK 5) (Apoptosis
signal-regulating kinase 1) (ASK-1)
Length = 1374
Score = 109 bits (273), Expect = 8e-24
Identities = 71/181 (39%), Positives = 100/181 (55%), Gaps = 4/181 (2%)
Frame = +3
Query: 258 ETIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI*IGANILI-TKQDCIKIV 434
+TI YTKQIL GLK+LH + I+HRDIK G N+LI T +KI
Sbjct: 778 QTIGFYTKQILEGLKYLHDNQIVHRDIK----------------GDNVLINTYSGVLKIS 821
Query: 435 DFGLSRFLQTVSRLEKKTFCGTVHYTSPEVIN--DESYGFPADIWSVGATVFEMATGRPP 608
DFG S+ L ++ +TF GT+ Y +PE+I+ YG ADIWS+G T+ EMATG+PP
Sbjct: 822 DFGTSKRLAGINPC-TETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPP 880
Query: 609 YHSAKNEIAVAVKIATTK-RLDYSKTLEDNKETKQFIDLMTKADPNKRKTADQLLNYHYL 785
++ A K+ K + +++ + E K FI + DP+KR A+ LL +L
Sbjct: 881 FYELGEPQAAMFKVGMFKVHPEIPESM--SAEAKAFILKCFEPDPDKRACANDLLVDEFL 938
Query: 786 K 788
K
Sbjct: 939 K 939
>sp|P23561|STE11_YEAST Serine/threonine-protein kinase STE11
Length = 738
Score = 102 bits (254), Expect = 1e-21
Identities = 69/195 (35%), Positives = 100/195 (51%), Gaps = 2/195 (1%)
Frame = +3
Query: 207 GSISEKISLNNYQPLPVETIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI* 386
GS+S LNNY P I +T+QIL G+ +LH+ NIIHRDIK
Sbjct: 560 GSVSSM--LNNYGPFEESLITNFTRQILIGVAYLHKKNIIHRDIK--------------- 602
Query: 387 IGANILITKQDCIKIVDFGLSRFLQTVSRLEKK--TFCGTVHYTSPEVINDESYGFPADI 560
GANILI + C+KI DFG+S+ L +++ + K + G+V + SPEV+ + ADI
Sbjct: 603 -GANILIDIKGCVKITDFGISKKLSPLNKKQNKRASLQGSVFWMSPEVVKQTATTAKADI 661
Query: 561 WSVGATVFEMATGRPPYHSAKNEIAVAVKIATTKRLDYSKTLEDNKETKQFIDLMTKADP 740
WS G V EM TG+ P+ +++ KI T + E K F+ + D
Sbjct: 662 WSTGCVVIEMFTGKHPFPDF-SQMQAIFKIGTNTTPEIPSWA--TSEGKNFLRKAFELDY 718
Query: 741 NKRKTADQLLNYHYL 785
R +A +LL + +L
Sbjct: 719 QYRPSALELLQHPWL 733
>sp|O95382|M3K6_HUMAN Mitogen-activated protein kinase kinase kinase 6
Length = 1011
Score = 102 bits (254), Expect = 1e-21
Identities = 67/179 (37%), Positives = 95/179 (53%), Gaps = 3/179 (1%)
Frame = +3
Query: 261 TIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI*IGANILI-TKQDCIKIVD 437
TI YT+QIL GL +LH ++I+HRDIK G N+LI T +KI D
Sbjct: 470 TISFYTRQILQGLGYLHDNHIVHRDIK----------------GDNVLINTFSGLLKISD 513
Query: 438 FGLSRFLQTVSRLEKKTFCGTVHYTSPEVIND--ESYGFPADIWSVGATVFEMATGRPPY 611
FG S+ L ++ + TF GT+ Y +PE+I+ YG ADIWS+G TV EMATGRPP+
Sbjct: 514 FGTSKRLAGITPCTE-TFTGTLQYMAPEIIDQGPRGYGKAADIWSLGCTVIEMATGRPPF 572
Query: 612 HSAKNEIAVAVKIATTKRLDYSKTLEDNKETKQFIDLMTKADPNKRKTADQLLNYHYLK 788
H + A ++ K + + E + F+ + DP R +A LL +L+
Sbjct: 573 HELGSPQAAMFQVGMYK-VHPPMPSSLSAEAQAFLLRTFEPDPRLRASAQTLLGDPFLQ 630
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 100 bits (250), Expect = 4e-21
Identities = 65/191 (34%), Positives = 100/191 (52%), Gaps = 2/191 (1%)
Frame = +3
Query: 216 SEKISLNNYQPLPVETIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI*IGA 395
S K L Y L + YT+QIL G+ +LH + I+HRDIK GA
Sbjct: 450 SVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIK----------------GA 493
Query: 396 NILITKQDCIKIVDFGLSRFLQTV--SRLEKKTFCGTVHYTSPEVINDESYGFPADIWSV 569
NIL +K+ DFG S+ LQT+ S ++ GT ++ SPEVI+ E YG AD+WS+
Sbjct: 494 NILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVTGTPYWMSPEVISGEGYGRKADVWSL 553
Query: 570 GATVFEMATGRPPYHSAKNEIAVAVKIATTKRLDYSKTLEDNKETKQFIDLMTKADPNKR 749
G TV EM T +PP+ A+ E A+ T+ + ++ + F+ + + +R
Sbjct: 554 GCTVVEMLTEKPPW--AEYEAMAAIFKIATQPTNPQLPSHISEHGRDFLRRIF-VEARQR 610
Query: 750 KTADQLLNYHY 782
+A++LL +H+
Sbjct: 611 PSAEELLTHHF 621
>sp|O08648|M3K4_MOUSE Mitogen-activated protein kinase kinase kinase 4 (MAPK/ERK kinase
kinase 4) (MEK kinase 4) (MEKK 4)
Length = 1597
Score = 100 bits (249), Expect = 5e-21
Identities = 65/186 (34%), Positives = 94/186 (50%), Gaps = 6/186 (3%)
Frame = +3
Query: 249 LPVETIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI*IGANILITKQDCIK 428
L I+LYTKQI + LH+ I+HRDIK GANI +T IK
Sbjct: 1424 LQEHVIRLYTKQITVAINVLHEHGIVHRDIK----------------GANIFLTSSGLIK 1467
Query: 429 IVDFGLSRFLQTVSRL---EKKTFCGTVHYTSPEVIN---DESYGFPADIWSVGATVFEM 590
+ DFG S L+ ++ E + GT Y +PEVI E +G ADIWS+G V EM
Sbjct: 1468 LGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEM 1527
Query: 591 ATGRPPYHSAKNEIAVAVKIATTKRLDYSKTLEDNKETKQFIDLMTKADPNKRKTADQLL 770
TG+ P+H ++ + K+ + + L + E K F+ ++DP R TA QLL
Sbjct: 1528 VTGKRPWHEYEHNFQIMYKVGMGHKPPIPERL--SPEGKAFLSHCLESDPKIRWTASQLL 1585
Query: 771 NYHYLK 788
++ ++K
Sbjct: 1586 DHAFVK 1591
>sp|Q9Y6R4|M3K4_HUMAN Mitogen-activated protein kinase kinase kinase 4 (MAPK/ERK kinase
kinase 4) (MEK kinase 4) (MEKK 4) (MAP three kinase 1)
Length = 1607
Score = 100 bits (249), Expect = 5e-21
Identities = 64/186 (34%), Positives = 94/186 (50%), Gaps = 6/186 (3%)
Frame = +3
Query: 249 LPVETIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI*IGANILITKQDCIK 428
L I+LY+KQI + LH+ I+HRDIK GANI +T IK
Sbjct: 1434 LQEHVIRLYSKQITIAINVLHEHGIVHRDIK----------------GANIFLTSSGLIK 1477
Query: 429 IVDFGLSRFLQTVSRL---EKKTFCGTVHYTSPEVIN---DESYGFPADIWSVGATVFEM 590
+ DFG S L+ ++ E + GT Y +PEVI E +G ADIWS+G V EM
Sbjct: 1478 LGDFGCSVKLKNNAQTMPGEVNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCVVIEM 1537
Query: 591 ATGRPPYHSAKNEIAVAVKIATTKRLDYSKTLEDNKETKQFIDLMTKADPNKRKTADQLL 770
TG+ P+H ++ + K+ + + L + E K F+ ++DP R TA QLL
Sbjct: 1538 VTGKRPWHEYEHNFQIMYKVGMGHKPPIPERL--SPEGKDFLSHCLESDPKMRWTASQLL 1595
Query: 771 NYHYLK 788
++ ++K
Sbjct: 1596 DHSFVK 1601
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 100 bits (248), Expect = 6e-21
Identities = 65/191 (34%), Positives = 100/191 (52%), Gaps = 2/191 (1%)
Frame = +3
Query: 216 SEKISLNNYQPLPVETIKLYTKQILSGLKFLHQSNIIHRDIKSIYLFKSSLFDFLI*IGA 395
S K L Y L + YT+QIL G+ +LH + I+HRDIK GA
Sbjct: 450 SVKDQLKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRDIK----------------GA 493
Query: 396 NILITKQDCIKIVDFGLSRFLQTV--SRLEKKTFCGTVHYTSPEVINDESYGFPADIWSV 569
NIL +K+ DFG S+ LQT+ S ++ GT ++ SPEVI+ E YG AD+WS+
Sbjct: 494 NILRDSAGNVKLGDFGASKRLQTICMSGTGIRSVTGTPYWMSPEVISGEGYGRKADVWSL 553
Query: 570 GATVFEMATGRPPYHSAKNEIAVAVKIATTKRLDYSKTLEDNKETKQFIDLMTKADPNKR 749
G TV EM T +PP+ A+ E A+ T+ + ++ + F+ + + +R
Sbjct: 554 GCTVVEMLTEKPPW--AEYEAMAAIFKIATQPTNPQLPSHISEHGRDFLRRIF-VEARQR 610
Query: 750 KTADQLLNYHY 782
+A++LL +H+
Sbjct: 611 PSAEELLTHHF 621
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 73,949,670
Number of Sequences: 369166
Number of extensions: 1323346
Number of successful extensions: 7395
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4849
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5076
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7666799535
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)