Planaria EST Database

DrC_01135

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01135
         (801 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q96T60|PNKP_HUMAN  Bifunctional polynucleotide phosphatas...   139   9e-33
sp|Q9JLV6|PNKP_MOUSE  Bifunctional polynucleotide phosphatas...   136   8e-32
sp|Q19683|YZR5_CAEEL  Hypothetical protein F21D5.5 in chromo...   124   2e-28
sp|O13911|PNK1_SCHPO  Bifunctional polynucleotide phosphatas...    70   7e-12
sp|P21286|Y033_NPVAC  Hypothetical 20.8 kDa protein in FGF-V...    40   0.010
sp|Q9Y6K8|KAD5_HUMAN  Adenylate kinase isoenzyme 5 (ATP-AMP ...    34   0.53 
sp|P06855|KIPN_BPT4  Polynucleotide kinase (PNK) (Polynucleo...    33   0.91 
sp|P41476|PNKL_NPVAC  Putative bifunctional polynucleotide k...    33   1.2  
sp|Q03796|TPP1_YEAST  Polynucleotide 3'-phosphatase (2'(3')-...    33   1.2  
sp|Q920P5|KAD5_MOUSE  Adenylate kinase isoenzyme 5 (ATP-AMP ...    33   1.2  

>sp|Q96T60|PNKP_HUMAN Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
           kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
           [Includes: Polynucleotide 3'-phosphatase
           (2'(3')-polynucleotidase); Polynucleotide
           5'-hydroxyl-kinase ]
          Length = 521

 Score =  139 bits (350), Expect = 9e-33
 Identities = 83/201 (41%), Positives = 112/201 (55%), Gaps = 1/201 (0%)
 Frame = -3

Query: 601 AGRVADWAP-KMKKDFSNSDRLFALNCHLKFYTPEAFFLQQEEGEFKMPEFHPAETVKNC 425
           AGR A+WAP + KKDFS +DRLFALN  L F TPE FFL+     F++P F P  TV   
Sbjct: 291 AGRPANWAPGRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDP-RTVSRS 349

Query: 424 PTLMIKYDKFQKSASDIKDILPKDSSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRD 245
             L +   +   SAS              +++ VG+PG+GKSTF KK+L   G+  +NRD
Sbjct: 350 GPLCLPESRALLSASP------------EVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRD 397

Query: 244 TLKTWQKCVAETQSLFXXXXXXXXXNTNPDLATRDRYISAAVECGVKHIICCIMDVSEDQ 65
           TL +WQ+CV   ++           NTNPD A+R RY+  A   GV    C +   + +Q
Sbjct: 398 TLGSWQRCVTTCETALKQGKRVAIDNTNPDAASRARYVQCARAAGVP-CRCFLFTATLEQ 456

Query: 64  SRHNEHFRQLTADHHDHVSEM 2
           +RHN  FR++T   H  VS+M
Sbjct: 457 ARHNNRFREMTDSSHIPVSDM 477

>sp|Q9JLV6|PNKP_MOUSE Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
           kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
           [Includes: Polynucleotide 3'-phosphatase
           (2'(3')-polynucleotidase); Polynucleotide
           5'-hydroxyl-kinase ]
          Length = 522

 Score =  136 bits (342), Expect = 8e-32
 Identities = 84/201 (41%), Positives = 118/201 (58%), Gaps = 1/201 (0%)
 Frame = -3

Query: 601 AGRVADWAP-KMKKDFSNSDRLFALNCHLKFYTPEAFFLQQEEGEFKMPEFHPAETVKNC 425
           AGR+A+WAP + KKDFS +DRLFALN  L F TPE FFL+     F++P F P  T+ + 
Sbjct: 290 AGRLANWAPGRKKKDFSCADRLFALNVGLPFATPEEFFLKWPAARFELPAFDP-RTISSA 348

Query: 424 PTLMIKYDKFQKSASDIKDILPKDSSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRD 245
             L +      +S+S     L   + EVV+   VG+PG+GKSTF +++L   G+  +NRD
Sbjct: 349 GPLYLP-----ESSS-----LLSPNPEVVVA--VGFPGAGKSTFIQEHLVSAGYVHVNRD 396

Query: 244 TLKTWQKCVAETQSLFXXXXXXXXXNTNPDLATRDRYISAAVECGVKHIICCIMDVSEDQ 65
           TL +WQ+CV+  Q+           NTNPD+ +R RYI  A + GV    C     + +Q
Sbjct: 397 TLGSWQRCVSSCQAALRQGKRVVIDNTNPDVPSRARYIQCAKDAGVP-CRCFNFCATIEQ 455

Query: 64  SRHNEHFRQLTADHHDHVSEM 2
           +RHN  FR++T   H  VS+M
Sbjct: 456 ARHNNRFREMTDPSHAPVSDM 476

>sp|Q19683|YZR5_CAEEL Hypothetical protein F21D5.5 in chromosome IV
          Length = 407

 Score =  124 bits (312), Expect = 2e-28
 Identities = 83/204 (40%), Positives = 110/204 (53%), Gaps = 4/204 (1%)
 Frame = -3

Query: 601 AGRVADWAPKMKKDFSNSDRLFALNCHLKFYTPEAFFLQQEEGEFKMPEFHPAETVKNCP 422
           AGR+   + + KKD S +DR FA N  +KF TPE FF     G+ K+ E          P
Sbjct: 176 AGRIKTTS-RPKKDHSYADRFFAANVGVKFQTPEEFF-----GKSKVDE----------P 219

Query: 421 TLMIKYDKFQKSASDIKDILPKD----SSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVI 254
                +D     + +I ++ P D    SSE  +ILMVG+PGSGKSTF K       + ++
Sbjct: 220 WGPPNFDPKNLFSEEITELEPHDAQLKSSEKEIILMVGFPGSGKSTFAKMLGHQHDYKIV 279

Query: 253 NRDTLKTWQKCVAETQSLFXXXXXXXXXNTNPDLATRDRYISAAVECGVKHIICCIMDVS 74
           NRDT+ TWQKCVA T+S           NT+PDL +R RYI  A E GV  I C  M+ S
Sbjct: 280 NRDTIGTWQKCVAATRSYLADGKSVVIDNTSPDLESRKRYIDVAKELGVP-IRCFEMNCS 338

Query: 73  EDQSRHNEHFRQLTADHHDHVSEM 2
            + ++HN  FR LT D+   +S M
Sbjct: 339 MEHAQHNIRFRVLTDDNAAEISSM 362

>sp|O13911|PNK1_SCHPO Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
           kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
           [Includes: Polynucleotide 3'-phosphatase
           (2'(3')-polynucleotidase); Polynucleotide
           5'-hydroxy-kinase ]
          Length = 421

 Score = 70.1 bits (170), Expect = 7e-12
 Identities = 57/196 (29%), Positives = 86/196 (43%), Gaps = 14/196 (7%)
 Frame = -3

Query: 562 DFSNSDRLFALNCHLKFYTPEAFFLQQEEGEFKMPEFHPAE-TVKNCPTLMIKYDKFQKS 386
           D +++D  FA N  +KF TPE FFL           FHP    V+N  +    + K    
Sbjct: 197 DHNSTDLKFAENIGIKFETPEQFFLGHSFVPPNFESFHPKNYLVRNSSSHPYHFKK---- 252

Query: 385 ASDIKDILPKDSSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRDTLKTWQKCV-AET 209
                      S    ++++VG+P SGKST  +  +   G+  +N+D LKT  KC+ A  
Sbjct: 253 -----------SEHQEIVVLVGFPSSGKSTLAESQIVTQGYERVNQDILKTKSKCIKAAI 301

Query: 208 QSL------------FXXXXXXXXXNTNPDLATRDRYISAAVECGVKHIICCIMDVSEDQ 65
           ++L                      NTNP + +R  +I  A E  +  I C  +  SE+ 
Sbjct: 302 EALKKEKSVVIGMYSIISTTYAISDNTNPTIESRKMWIDIAQEFEIP-IRCIHLQSSEEL 360

Query: 64  SRHNEHFRQLTADHHD 17
           +RHN  FR +   HH+
Sbjct: 361 ARHNNVFRYI---HHN 373

>sp|P21286|Y033_NPVAC Hypothetical 20.8 kDa protein in FGF-VUBI intergenic region (ORF 1)
          Length = 182

 Score = 39.7 bits (91), Expect = 0.010
 Identities = 17/25 (68%), Positives = 21/25 (84%)
 Frame = -3

Query: 568 KKDFSNSDRLFALNCHLKFYTPEAF 494
           +KDFS+SDR+FA N  L+FYTPE F
Sbjct: 151 QKDFSDSDRVFAKNLSLQFYTPEQF 175

>sp|Q9Y6K8|KAD5_HUMAN Adenylate kinase isoenzyme 5 (ATP-AMP transphosphorylase)
          Length = 198

 Score = 33.9 bits (76), Expect = 0.53
 Identities = 15/40 (37%), Positives = 24/40 (60%)
 Frame = -3

Query: 358 KDSSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRDTL 239
           +D  +  +I ++G PGSGK T C+K +E  GF  ++   L
Sbjct: 6   EDLRKCKIIFIIGGPGSGKGTQCEKLVEKYGFTHLSTGEL 45

>sp|P06855|KIPN_BPT4 Polynucleotide kinase (PNK) (Polynucleotide 5'-hydroxy-kinase)
          Length = 301

 Score = 33.1 bits (74), Expect = 0.91
 Identities = 16/32 (50%), Positives = 24/32 (75%), Gaps = 1/32 (3%)
 Frame = -3

Query: 337 LILMVGYPGSGKSTFCKKYL-EPLGFNVINRD 245
           +IL +G PGSGKST+ ++++ +  GF  INRD
Sbjct: 4   IILTIGCPGSGKSTWAREFIAKNPGFYNINRD 35

>sp|P41476|PNKL_NPVAC Putative bifunctional polynucleotide kinase/RNA ligase [Includes:
           Polynucleotide kinase (PNK) (Polynucleotide
           5'-hydroxy-kinase); RNA ligase ]
          Length = 694

 Score = 32.7 bits (73), Expect = 1.2
 Identities = 25/82 (30%), Positives = 43/82 (52%), Gaps = 1/82 (1%)
 Frame = -3

Query: 478 EGEF-KMPEFHPAETVKNCPTLMIKYDKFQKSASDIKDILPKDSSEVVLILMVGYPGSGK 302
           E EF K+ E    + +KN    +IK+   +++ ++      K  S   L++++G  GSGK
Sbjct: 352 ESEFLKLTEPEKIKMIKN----IIKFVSTKQALNNKLAPTIKLPSSKQLLVLIGISGSGK 407

Query: 301 STFCKKYLEPLGFNVINRDTLK 236
           ST+ K      G+  INRD ++
Sbjct: 408 STYAKSL---KGYTEINRDDVR 426

>sp|Q03796|TPP1_YEAST Polynucleotide 3'-phosphatase (2'(3')-polynucleotidase) (DNA 3'
           phosphatase) (Three prime phosphatase)
          Length = 238

 Score = 32.7 bits (73), Expect = 1.2
 Identities = 15/26 (57%), Positives = 18/26 (69%)
 Frame = -3

Query: 568 KKDFSNSDRLFALNCHLKFYTPEAFF 491
           KKDFS+SD  FA N H++F  PE  F
Sbjct: 211 KKDFSDSDIKFAENLHVEFKYPEEIF 236

>sp|Q920P5|KAD5_MOUSE Adenylate kinase isoenzyme 5 (ATP-AMP transphosphorylase)
          Length = 193

 Score = 32.7 bits (73), Expect = 1.2
 Identities = 15/40 (37%), Positives = 23/40 (57%)
 Frame = -3

Query: 358 KDSSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRDTL 239
           +D  +  +I ++G PGSGK T C+K  E  GF  ++   L
Sbjct: 2   EDLRKCKIIFLMGGPGSGKGTQCEKLAEKYGFTHLSTGEL 41

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,285,553
Number of Sequences: 369166
Number of extensions: 1728193
Number of successful extensions: 5281
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5112
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5272
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7570361805
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_01135

  1. Dr_sW_028_J11
  2. Dr_sW_023_G08
  3. Dr_sW_001_F12
  4. Dr_sW_006_C21
  5. Dr_sW_015_C11