Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_J11
(798 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q96T60|PNKP_HUMAN Bifunctional polynucleotide phosphatas... 139 9e-33
sp|Q9JLV6|PNKP_MOUSE Bifunctional polynucleotide phosphatas... 136 8e-32
sp|Q19683|YZR5_CAEEL Hypothetical protein F21D5.5 in chromo... 124 2e-28
sp|O13911|PNK1_SCHPO Bifunctional polynucleotide phosphatas... 70 7e-12
sp|P21286|Y033_NPVAC Hypothetical 20.8 kDa protein in FGF-V... 40 0.010
sp|Q9Y6K8|KAD5_HUMAN Adenylate kinase isoenzyme 5 (ATP-AMP ... 34 0.53
sp|P06855|KIPN_BPT4 Polynucleotide kinase (PNK) (Polynucleo... 33 0.90
sp|P41476|PNKL_NPVAC Putative bifunctional polynucleotide k... 33 1.2
sp|Q03796|TPP1_YEAST Polynucleotide 3'-phosphatase (2'(3')-... 33 1.2
sp|Q920P5|KAD5_MOUSE Adenylate kinase isoenzyme 5 (ATP-AMP ... 33 1.2
>sp|Q96T60|PNKP_HUMAN Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
[Includes: Polynucleotide 3'-phosphatase
(2'(3')-polynucleotidase); Polynucleotide
5'-hydroxyl-kinase ]
Length = 521
Score = 139 bits (350), Expect = 9e-33
Identities = 82/201 (40%), Positives = 111/201 (55%), Gaps = 1/201 (0%)
Frame = +3
Query: 198 AGRVADWAP-KMKKDFSNSDRLFALNCHLKFYTPEAFFLQQEEGEFKMPEFHPAETVKNC 374
AGR A+WAP + KKDFS +DRLFALN L F TPE FFL+ F++P F P TV
Sbjct: 291 AGRPANWAPGRKKKDFSCADRLFALNLGLPFATPEEFFLKWPAAGFELPAFDP-RTVSRS 349
Query: 375 PTLMIKYDKFQKSASDIKDILPKDSSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRD 554
L + + SAS +++ VG+PG+GKSTF KK+L G+ +NRD
Sbjct: 350 GPLCLPESRALLSASP------------EVVVAVGFPGAGKSTFLKKHLVSAGYVHVNRD 397
Query: 555 TLKTWQKCVAETQSLFXXXXXXXXXXTNPDLATRDRYISAAVECGVKHIICCIMDVSEDQ 734
TL +WQ+CV ++ TNPD A+R RY+ A GV C + + +Q
Sbjct: 398 TLGSWQRCVTTCETALKQGKRVAIDNTNPDAASRARYVQCARAAGVP-CRCFLFTATLEQ 456
Query: 735 SRHNEHFRQLTADHHDHVSEM 797
+RHN FR++T H VS+M
Sbjct: 457 ARHNNRFREMTDSSHIPVSDM 477
>sp|Q9JLV6|PNKP_MOUSE Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
[Includes: Polynucleotide 3'-phosphatase
(2'(3')-polynucleotidase); Polynucleotide
5'-hydroxyl-kinase ]
Length = 522
Score = 136 bits (342), Expect = 8e-32
Identities = 83/201 (41%), Positives = 117/201 (58%), Gaps = 1/201 (0%)
Frame = +3
Query: 198 AGRVADWAP-KMKKDFSNSDRLFALNCHLKFYTPEAFFLQQEEGEFKMPEFHPAETVKNC 374
AGR+A+WAP + KKDFS +DRLFALN L F TPE FFL+ F++P F P T+ +
Sbjct: 290 AGRLANWAPGRKKKDFSCADRLFALNVGLPFATPEEFFLKWPAARFELPAFDP-RTISSA 348
Query: 375 PTLMIKYDKFQKSASDIKDILPKDSSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRD 554
L + +S+S L + EVV+ VG+PG+GKSTF +++L G+ +NRD
Sbjct: 349 GPLYLP-----ESSS-----LLSPNPEVVVA--VGFPGAGKSTFIQEHLVSAGYVHVNRD 396
Query: 555 TLKTWQKCVAETQSLFXXXXXXXXXXTNPDLATRDRYISAAVECGVKHIICCIMDVSEDQ 734
TL +WQ+CV+ Q+ TNPD+ +R RYI A + GV C + +Q
Sbjct: 397 TLGSWQRCVSSCQAALRQGKRVVIDNTNPDVPSRARYIQCAKDAGVP-CRCFNFCATIEQ 455
Query: 735 SRHNEHFRQLTADHHDHVSEM 797
+RHN FR++T H VS+M
Sbjct: 456 ARHNNRFREMTDPSHAPVSDM 476
>sp|Q19683|YZR5_CAEEL Hypothetical protein F21D5.5 in chromosome IV
Length = 407
Score = 124 bits (312), Expect = 2e-28
Identities = 82/204 (40%), Positives = 109/204 (53%), Gaps = 4/204 (1%)
Frame = +3
Query: 198 AGRVADWAPKMKKDFSNSDRLFALNCHLKFYTPEAFFLQQEEGEFKMPEFHPAETVKNCP 377
AGR+ + + KKD S +DR FA N +KF TPE FF G+ K+ E P
Sbjct: 176 AGRIKTTS-RPKKDHSYADRFFAANVGVKFQTPEEFF-----GKSKVDE----------P 219
Query: 378 TLMIKYDKFQKSASDIKDILPKD----SSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVI 545
+D + +I ++ P D SSE +ILMVG+PGSGKSTF K + ++
Sbjct: 220 WGPPNFDPKNLFSEEITELEPHDAQLKSSEKEIILMVGFPGSGKSTFAKMLGHQHDYKIV 279
Query: 546 NRDTLKTWQKCVAETQSLFXXXXXXXXXXTNPDLATRDRYISAAVECGVKHIICCIMDVS 725
NRDT+ TWQKCVA T+S T+PDL +R RYI A E GV I C M+ S
Sbjct: 280 NRDTIGTWQKCVAATRSYLADGKSVVIDNTSPDLESRKRYIDVAKELGVP-IRCFEMNCS 338
Query: 726 EDQSRHNEHFRQLTADHHDHVSEM 797
+ ++HN FR LT D+ +S M
Sbjct: 339 MEHAQHNIRFRVLTDDNAAEISSM 362
>sp|O13911|PNK1_SCHPO Bifunctional polynucleotide phosphatase/kinase (Polynucleotide
kinase-3'-phosphatase) (DNA 5'-kinase/3'-phosphatase)
[Includes: Polynucleotide 3'-phosphatase
(2'(3')-polynucleotidase); Polynucleotide
5'-hydroxy-kinase ]
Length = 421
Score = 70.1 bits (170), Expect = 7e-12
Identities = 56/196 (28%), Positives = 85/196 (43%), Gaps = 14/196 (7%)
Frame = +3
Query: 237 DFSNSDRLFALNCHLKFYTPEAFFLQQEEGEFKMPEFHPAE-TVKNCPTLMIKYDKFQKS 413
D +++D FA N +KF TPE FFL FHP V+N + + K
Sbjct: 197 DHNSTDLKFAENIGIKFETPEQFFLGHSFVPPNFESFHPKNYLVRNSSSHPYHFKK---- 252
Query: 414 ASDIKDILPKDSSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRDTLKTWQKCV-AET 590
S ++++VG+P SGKST + + G+ +N+D LKT KC+ A
Sbjct: 253 -----------SEHQEIVVLVGFPSSGKSTLAESQIVTQGYERVNQDILKTKSKCIKAAI 301
Query: 591 QSL------------FXXXXXXXXXXTNPDLATRDRYISAAVECGVKHIICCIMDVSEDQ 734
++L TNP + +R +I A E + I C + SE+
Sbjct: 302 EALKKEKSVVIGMYSIISTTYAISDNTNPTIESRKMWIDIAQEFEIP-IRCIHLQSSEEL 360
Query: 735 SRHNEHFRQLTADHHD 782
+RHN FR + HH+
Sbjct: 361 ARHNNVFRYI---HHN 373
>sp|P21286|Y033_NPVAC Hypothetical 20.8 kDa protein in FGF-VUBI intergenic region (ORF 1)
Length = 182
Score = 39.7 bits (91), Expect = 0.010
Identities = 17/25 (68%), Positives = 21/25 (84%)
Frame = +3
Query: 231 KKDFSNSDRLFALNCHLKFYTPEAF 305
+KDFS+SDR+FA N L+FYTPE F
Sbjct: 151 QKDFSDSDRVFAKNLSLQFYTPEQF 175
>sp|Q9Y6K8|KAD5_HUMAN Adenylate kinase isoenzyme 5 (ATP-AMP transphosphorylase)
Length = 198
Score = 33.9 bits (76), Expect = 0.53
Identities = 15/40 (37%), Positives = 24/40 (60%)
Frame = +3
Query: 441 KDSSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRDTL 560
+D + +I ++G PGSGK T C+K +E GF ++ L
Sbjct: 6 EDLRKCKIIFIIGGPGSGKGTQCEKLVEKYGFTHLSTGEL 45
>sp|P06855|KIPN_BPT4 Polynucleotide kinase (PNK) (Polynucleotide 5'-hydroxy-kinase)
Length = 301
Score = 33.1 bits (74), Expect = 0.90
Identities = 16/32 (50%), Positives = 24/32 (75%), Gaps = 1/32 (3%)
Frame = +3
Query: 462 LILMVGYPGSGKSTFCKKYL-EPLGFNVINRD 554
+IL +G PGSGKST+ ++++ + GF INRD
Sbjct: 4 IILTIGCPGSGKSTWAREFIAKNPGFYNINRD 35
>sp|P41476|PNKL_NPVAC Putative bifunctional polynucleotide kinase/RNA ligase [Includes:
Polynucleotide kinase (PNK) (Polynucleotide
5'-hydroxy-kinase); RNA ligase ]
Length = 694
Score = 32.7 bits (73), Expect = 1.2
Identities = 25/82 (30%), Positives = 43/82 (52%), Gaps = 1/82 (1%)
Frame = +3
Query: 321 EGEF-KMPEFHPAETVKNCPTLMIKYDKFQKSASDIKDILPKDSSEVVLILMVGYPGSGK 497
E EF K+ E + +KN +IK+ +++ ++ K S L++++G GSGK
Sbjct: 352 ESEFLKLTEPEKIKMIKN----IIKFVSTKQALNNKLAPTIKLPSSKQLLVLIGISGSGK 407
Query: 498 STFCKKYLEPLGFNVINRDTLK 563
ST+ K G+ INRD ++
Sbjct: 408 STYAKSL---KGYTEINRDDVR 426
>sp|Q03796|TPP1_YEAST Polynucleotide 3'-phosphatase (2'(3')-polynucleotidase) (DNA 3'
phosphatase) (Three prime phosphatase)
Length = 238
Score = 32.7 bits (73), Expect = 1.2
Identities = 15/26 (57%), Positives = 18/26 (69%)
Frame = +3
Query: 231 KKDFSNSDRLFALNCHLKFYTPEAFF 308
KKDFS+SD FA N H++F PE F
Sbjct: 211 KKDFSDSDIKFAENLHVEFKYPEEIF 236
>sp|Q920P5|KAD5_MOUSE Adenylate kinase isoenzyme 5 (ATP-AMP transphosphorylase)
Length = 193
Score = 32.7 bits (73), Expect = 1.2
Identities = 15/40 (37%), Positives = 23/40 (57%)
Frame = +3
Query: 441 KDSSEVVLILMVGYPGSGKSTFCKKYLEPLGFNVINRDTL 560
+D + +I ++G PGSGK T C+K E GF ++ L
Sbjct: 2 EDLRKCKIIFLMGGPGSGKGTQCEKLAEKYGFTHLSTGEL 41
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,042,573
Number of Sequences: 369166
Number of extensions: 1726197
Number of successful extensions: 5281
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5112
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5272
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7522142940
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)