Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= DrC_01106 (796 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|P52912|TIA1_MOUSE Nucleolysin TIA-1 (RNA-binding protein... 102 9e-22 sp|P31483|TIA1_HUMAN Nucleolysin TIA-1 isoform p40 (RNA-bin... 102 9e-22 sp|P70318|TIAR_MOUSE Nucleolysin TIAR (TIA-1 related protein) 101 2e-21 sp|Q01085|TIAR_HUMAN Nucleolysin TIAR (TIA-1 related protein) 100 3e-21 sp|O60176|YG41_SCHPO Hypothetical RNA-binding protein C23E6... 59 2e-08 sp|Q09702|NRD1_SCHPO Negative regulator of differentiation ... 56 1e-07 sp|P32588|PUB1_YEAST Nuclear and cytoplasmic polyadenylated... 55 3e-07 sp|Q00539|NAM8_YEAST NAM8 protein 52 1e-06 sp|Q8SX83|SPEN_DROME Split ends protein 50 9e-06 sp|P92204|NELFE_DROME Negative elongation factor E 48 4e-05
>sp|P52912|TIA1_MOUSE Nucleolysin TIA-1 (RNA-binding protein TIA-1) Length = 386 Score = 102 bits (255), Expect = 9e-22 Identities = 56/143 (39%), Positives = 79/143 (55%), Gaps = 2/143 (1%) Frame = +2 Query: 8 DAERAIEKMHKTWFHNRTIKCNWATRNGLDGDQFIKYTPRP--YELVYKESPLTNTNVYI 181 DAE AI++M W R I+ NWATR + + Y+ V +S N VY Sbjct: 159 DAENAIQQMGGQWLGGRQIRTNWATRKPPAPKSTYESNTKQLSYDEVVSQSSPNNCTVYC 218 Query: 182 AGIPEGLTEELVRVHFEEFGRIDAVKVYPEKAHAFINFVTHESAARAISQRHGYKINDNV 361 G+ GLTE+L+R F FG+I ++V+P+K ++F+ F +HESAA AI +G I +V Sbjct: 219 GGVTSGLTEQLMRQTFSPFGQIMEIRVFPDKGYSFVRFSSHESAAHAIVSVNGTTIEGHV 278 Query: 362 IKCNWGKENFGFSITPLPVQPAI 430 +KC WGKE I P+ Q I Sbjct: 279 VKCYWGKETLDM-INPVQQQNQI 300
>sp|P31483|TIA1_HUMAN Nucleolysin TIA-1 isoform p40 (RNA-binding protein TIA-1) (p40-TIA-1) [Contains: Nucleolysin TIA-1 isoform p15 (p15-TIA-1)] Length = 386 Score = 102 bits (255), Expect = 9e-22 Identities = 56/143 (39%), Positives = 80/143 (55%), Gaps = 2/143 (1%) Frame = +2 Query: 8 DAERAIEKMHKTWFHNRTIKCNWATRNGLDGDQFIKYTPRP--YELVYKESPLTNTNVYI 181 DAE AI++M W R I+ NWATR + + Y+ V +S +N VY Sbjct: 159 DAENAIQQMGGQWLGGRQIRTNWATRKPPAPKSTYESNTKQLSYDEVVNQSSPSNCTVYC 218 Query: 182 AGIPEGLTEELVRVHFEEFGRIDAVKVYPEKAHAFINFVTHESAARAISQRHGYKINDNV 361 G+ GLTE+L+R F FG+I ++V+P+K ++F+ F +HESAA AI +G I +V Sbjct: 219 GGVTSGLTEQLMRQTFSPFGQIMEIRVFPDKGYSFVRFNSHESAAHAIVSVNGTTIEGHV 278 Query: 362 IKCNWGKENFGFSITPLPVQPAI 430 +KC WGKE I P+ Q I Sbjct: 279 VKCYWGKETLDM-INPVQQQNQI 300
>sp|P70318|TIAR_MOUSE Nucleolysin TIAR (TIA-1 related protein) Length = 392 Score = 101 bits (252), Expect = 2e-21 Identities = 55/129 (42%), Positives = 72/129 (55%), Gaps = 2/129 (1%) Frame = +2 Query: 8 DAERAIEKMHKTWFHNRTIKCNWATRN--GLDGDQFIKYTPRPYELVYKESPLTNTNVYI 181 DAE AI M W R I+ NWATR Q +E V +S N VY Sbjct: 167 DAENAIVHMGGQWLGGRQIRTNWATRKPPAPKSTQETNTKQLRFEDVVNQSSPKNCTVYC 226 Query: 182 AGIPEGLTEELVRVHFEEFGRIDAVKVYPEKAHAFINFVTHESAARAISQRHGYKINDNV 361 GI GLT++L+R F FG+I ++V+PEK ++F+ F THESAA AI +G I +V Sbjct: 227 GGIASGLTDQLMRQTFSPFGQIMEIRVFPEKGYSFVRFSTHESAAHAIVSVNGTTIEGHV 286 Query: 362 IKCNWGKEN 388 +KC WGKE+ Sbjct: 287 VKCYWGKES 295
>sp|Q01085|TIAR_HUMAN Nucleolysin TIAR (TIA-1 related protein) Length = 375 Score = 100 bits (250), Expect = 3e-21 Identities = 55/129 (42%), Positives = 72/129 (55%), Gaps = 2/129 (1%) Frame = +2 Query: 8 DAERAIEKMHKTWFHNRTIKCNWATRN--GLDGDQFIKYTPRPYELVYKESPLTNTNVYI 181 DAE AI M W R I+ NWATR Q +E V +S N VY Sbjct: 150 DAENAIVHMGGQWLGGRQIRTNWATRKPPAPKSTQENNTKQLRFEDVVNQSSPKNCTVYC 209 Query: 182 AGIPEGLTEELVRVHFEEFGRIDAVKVYPEKAHAFINFVTHESAARAISQRHGYKINDNV 361 GI GLT++L+R F FG+I ++V+PEK ++F+ F THESAA AI +G I +V Sbjct: 210 GGIASGLTDQLMRQTFSPFGQIMEIRVFPEKGYSFVRFSTHESAAHAIVSVNGTTIEGHV 269 Query: 362 IKCNWGKEN 388 +KC WGKE+ Sbjct: 270 VKCYWGKES 278
>sp|O60176|YG41_SCHPO Hypothetical RNA-binding protein C23E6.01c in chromosome II Length = 473 Score = 58.5 bits (140), Expect = 2e-08 Identities = 26/73 (35%), Positives = 46/73 (63%) Frame = +2 Query: 164 NTNVYIAGIPEGLTEELVRVHFEEFGRIDAVKVYPEKAHAFINFVTHESAARAISQRHGY 343 N+ V++ G+ + ++EE ++ F+ FG I VK+ P K F+ FV +SA AI+Q GY Sbjct: 302 NSTVFVGGLSKFVSEEELKYLFQNFGEIVYVKIPPGKGCGFVQFVNRQSAEIAINQLQGY 361 Query: 344 KINDNVIKCNWGK 382 + ++ I+ +WG+ Sbjct: 362 PLGNSRIRLSWGR 374
>sp|Q09702|NRD1_SCHPO Negative regulator of differentiation 1 (Multicopy suppressor of sporulation protein msa2) Length = 529 Score = 56.2 bits (134), Expect = 1e-07 Identities = 37/127 (29%), Positives = 58/127 (45%), Gaps = 3/127 (2%) Frame = +2 Query: 23 IEKMHKTWFHNRTIKCNWATRNGLDGDQFIKYTPRPYELVYKESPLTNTNVYIAGIPEGL 202 + +H NR +K W +G P P + + + N+YI + L Sbjct: 376 MSNIHGLVIRNRRLKIGWGKHSG----------PLPSNIALAVAGGASRNIYIGNADDSL 425 Query: 203 TEELVRVHFEEFGRIDAVKVYPEKAHAFINFVTHESAARA---ISQRHGYKINDNVIKCN 373 T E ++ FEEFG I+ V + EK AF+NF + SA A I Q+ GY+ + + Sbjct: 426 TIERLKEDFEEFGEIEYVNFFREKNCAFVNFTSLASAINAIDRIKQKKGYE----NYRIS 481 Query: 374 WGKENFG 394 +GK+ G Sbjct: 482 YGKDRCG 488
Score = 47.8 bits (112), Expect = 4e-05 Identities = 19/54 (35%), Positives = 35/54 (64%) Frame = +2 Query: 170 NVYIAGIPEGLTEELVRVHFEEFGRIDAVKVYPEKAHAFINFVTHESAARAISQ 331 NV++ +P G+TE+ +R E FG ID +K+ E+ AF++F+ +A +A+ + Sbjct: 207 NVFLGNLPNGITEDEIREDLEPFGPIDQIKIVTERNIAFVHFLNIAAAIKAVQE 260
Score = 30.0 bits (66), Expect = 7.6 Identities = 23/85 (27%), Positives = 38/85 (44%), Gaps = 2/85 (2%) Frame = +2 Query: 173 VYIAGIPEGLTEELVRVHFEEFGRIDAVKVYPEKAHAFINFV--THESAARAISQRHGYK 346 VY+ +P + + + G I++ + PEK AFI+F+ +H +A + Sbjct: 117 VYVGNLPPNTPIDEI-LSCVRTGPIESAWILPEKNCAFISFLDPSHATAFFQDAALKRLT 175 Query: 347 INDNVIKCNWGKENFGFSITPLPVQ 421 I +K WGK + S L VQ Sbjct: 176 IRGTEVKVGWGKNSASNSSVLLAVQ 200
>sp|P32588|PUB1_YEAST Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1 (ARS consensus binding protein ACBP-60) (Poly(U)-binding protein) (Poly uridylate-binding protein) Length = 453 Score = 54.7 bits (130), Expect = 3e-07 Identities = 31/92 (33%), Positives = 42/92 (45%) Frame = +2 Query: 122 PRPYELVYKESPLTNTNVYIAGIPEGLTEELVRVHFEEFGRIDAVKVYPEKAHAFINFVT 301 P+ + + + +P T YI IP TE + F+ FG I K YPEK FI + T Sbjct: 326 PQAVDHIIRSAPPRVTTAYIGNIPHFATEADLIPLFQNFGFILDFKHYPEKGCCFIKYDT 385 Query: 302 HESAARAISQRHGYKINDNVIKCNWGKENFGF 397 HE AA I + ++ WGKE F Sbjct: 386 HEQAAVCIVALANFPFQGRNLRTGWGKERSNF 417
Score = 40.8 bits (94), Expect = 0.004 Identities = 18/73 (24%), Positives = 41/73 (56%), Gaps = 5/73 (6%) Frame = +2 Query: 173 VYIAGIPEGLTEELVRVHFEEFGRIDAVKVYPEK-----AHAFINFVTHESAARAISQRH 337 +Y+ + + +TE++++ +F+ G I +K+ +K +AF+ + A A+ + Sbjct: 77 LYVGNLDKAITEDILKQYFQVGGPIANIKIMIDKNNKNVNYAFVEYHQSHDANIALQTLN 136 Query: 338 GYKINDNVIKCNW 376 G +I +N++K NW Sbjct: 137 GKQIENNIVKINW 149
>sp|Q00539|NAM8_YEAST NAM8 protein Length = 523 Score = 52.4 bits (124), Expect = 1e-06 Identities = 25/73 (34%), Positives = 43/73 (58%) Frame = +2 Query: 164 NTNVYIAGIPEGLTEELVRVHFEEFGRIDAVKVYPEKAHAFINFVTHESAARAISQRHGY 343 NT V+I G+ +TE+ +R +F+ FG I VK+ K F+ +V SA AI+ G+ Sbjct: 312 NTTVFIGGLSSLVTEDELRAYFQPFGTIVYVKIPVGKCCGFVQYVDRLSAEAAIAGMQGF 371 Query: 344 KINDNVIKCNWGK 382 I ++ ++ +WG+ Sbjct: 372 PIANSRVRLSWGR 384
>sp|Q8SX83|SPEN_DROME Split ends protein Length = 5560 Score = 49.7 bits (117), Expect = 9e-06 Identities = 35/129 (27%), Positives = 59/129 (45%), Gaps = 3/129 (2%) Frame = +2 Query: 5 DDAERAIEKMHKTWFHNRTIKCNWATRNGLDGDQFIKYTPRPYELVYKE-SPLTNTNVYI 181 DD E+A+E H F I+ ++ ++F RPYE E P + ++I Sbjct: 606 DDVEKALEVSHDKHFFGCKIEVEPYQGYDVEDNEF-----RPYEAELDEYHPKSTRTLFI 660 Query: 182 AGIPEGLTEELVRVHFEEFGRIDAVKVYPE--KAHAFINFVTHESAARAISQRHGYKIND 355 + + +T +R HFE FG I + + + A+AF + S +A+ + G + Sbjct: 661 GNLEKDITAGELRSHFEAFGEIIEIDIKKQGLNAYAFCQYSDIVSVVKAMRKMDGEHLGS 720 Query: 356 NVIKCNWGK 382 N IK +GK Sbjct: 721 NRIKLGFGK 729
>sp|P92204|NELFE_DROME Negative elongation factor E Length = 280 Score = 47.8 bits (112), Expect = 4e-05 Identities = 23/75 (30%), Positives = 41/75 (54%) Frame = +2 Query: 128 PYELVYKESPLTNTNVYIAGIPEGLTEELVRVHFEEFGRIDAVKVYPEKAHAFINFVTHE 307 P + E P ++++G +TE+ ++ F ++G I V + EK+ F++F E Sbjct: 153 PMDTAQPEKPRAGNTIFVSG--NKVTEDFLKKTFNDYGTIVNVSMEIEKSRGFVSFAKPE 210 Query: 308 SAARAISQRHGYKIN 352 SA RAI++ HG +N Sbjct: 211 SADRAIAEIHGKNVN 225
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 86,037,812 Number of Sequences: 369166 Number of extensions: 1651487 Number of successful extensions: 4600 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 4394 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 4564 length of database: 68,354,980 effective HSP length: 109 effective length of database: 48,218,865 effective search space used: 7473924075 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)