Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_015_D11
(493 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P70318|TIAR_MOUSE Nucleolysin TIAR (TIA-1 related protein) 89 6e-18
sp|Q01085|TIAR_HUMAN Nucleolysin TIAR (TIA-1 related protein) 88 9e-18
sp|P52912|TIA1_MOUSE Nucleolysin TIA-1 (RNA-binding protein... 88 1e-17
sp|P31483|TIA1_HUMAN Nucleolysin TIA-1 isoform p40 (RNA-bin... 88 1e-17
sp|O60176|YG41_SCHPO Hypothetical RNA-binding protein C23E6... 59 8e-09
sp|P32588|PUB1_YEAST Nuclear and cytoplasmic polyadenylated... 55 1e-07
sp|Q00539|NAM8_YEAST NAM8 protein 52 6e-07
sp|Q09702|NRD1_SCHPO Negative regulator of differentiation ... 51 2e-06
sp|P92204|NELFE_DROME Negative elongation factor E 48 1e-05
sp|P70372|ELAV1_MOUSE ELAV-like protein 1 (Hu-antigen R) (H... 47 2e-05
>sp|P70318|TIAR_MOUSE Nucleolysin TIAR (TIA-1 related protein)
Length = 392
Score = 89.0 bits (219), Expect = 6e-18
Identities = 47/111 (42%), Positives = 64/111 (57%), Gaps = 2/111 (1%)
Frame = +1
Query: 4 IKCNWATRN--GLDGDQFIKYTPRPYELVYKESPLTNTNVYIAGIPEGLTEELVRVHFEE 177
I+ NWATR Q +E V +S N VY GI GLT++L+R F
Sbjct: 185 IRTNWATRKPPAPKSTQETNTKQLRFEDVVNQSSPKNCTVYCGGIASGLTDQLMRQTFSP 244
Query: 178 FGRIDAVKVYPEKAHAFINFVTHESAARAISQRHGYKINDNVIKCNWGKEN 330
FG+I ++V+PEK ++F+ F THESAA AI +G I +V+KC WGKE+
Sbjct: 245 FGQIMEIRVFPEKGYSFVRFSTHESAAHAIVSVNGTTIEGHVVKCYWGKES 295
>sp|Q01085|TIAR_HUMAN Nucleolysin TIAR (TIA-1 related protein)
Length = 375
Score = 88.2 bits (217), Expect = 9e-18
Identities = 47/111 (42%), Positives = 64/111 (57%), Gaps = 2/111 (1%)
Frame = +1
Query: 4 IKCNWATRN--GLDGDQFIKYTPRPYELVYKESPLTNTNVYIAGIPEGLTEELVRVHFEE 177
I+ NWATR Q +E V +S N VY GI GLT++L+R F
Sbjct: 168 IRTNWATRKPPAPKSTQENNTKQLRFEDVVNQSSPKNCTVYCGGIASGLTDQLMRQTFSP 227
Query: 178 FGRIDAVKVYPEKAHAFINFVTHESAARAISQRHGYKINDNVIKCNWGKEN 330
FG+I ++V+PEK ++F+ F THESAA AI +G I +V+KC WGKE+
Sbjct: 228 FGQIMEIRVFPEKGYSFVRFSTHESAAHAIVSVNGTTIEGHVVKCYWGKES 278
>sp|P52912|TIA1_MOUSE Nucleolysin TIA-1 (RNA-binding protein TIA-1)
Length = 386
Score = 87.8 bits (216), Expect = 1e-17
Identities = 48/125 (38%), Positives = 69/125 (55%), Gaps = 2/125 (1%)
Frame = +1
Query: 4 IKCNWATRNGLDGDQFIKYTPRP--YELVYKESPLTNTNVYIAGIPEGLTEELVRVHFEE 177
I+ NWATR + + Y+ V +S N VY G+ GLTE+L+R F
Sbjct: 177 IRTNWATRKPPAPKSTYESNTKQLSYDEVVSQSSPNNCTVYCGGVTSGLTEQLMRQTFSP 236
Query: 178 FGRIDAVKVYPEKAHAFINFVTHESAARAISQRHGYKINDNVIKCNWGKENFGFSITPLP 357
FG+I ++V+P+K ++F+ F +HESAA AI +G I +V+KC WGKE I P+
Sbjct: 237 FGQIMEIRVFPDKGYSFVRFSSHESAAHAIVSVNGTTIEGHVVKCYWGKETLDM-INPVQ 295
Query: 358 VQPAI 372
Q I
Sbjct: 296 QQNQI 300
>sp|P31483|TIA1_HUMAN Nucleolysin TIA-1 isoform p40 (RNA-binding protein TIA-1)
(p40-TIA-1) [Contains: Nucleolysin TIA-1 isoform p15
(p15-TIA-1)]
Length = 386
Score = 87.8 bits (216), Expect = 1e-17
Identities = 48/125 (38%), Positives = 70/125 (56%), Gaps = 2/125 (1%)
Frame = +1
Query: 4 IKCNWATRNGLDGDQFIKYTPRP--YELVYKESPLTNTNVYIAGIPEGLTEELVRVHFEE 177
I+ NWATR + + Y+ V +S +N VY G+ GLTE+L+R F
Sbjct: 177 IRTNWATRKPPAPKSTYESNTKQLSYDEVVNQSSPSNCTVYCGGVTSGLTEQLMRQTFSP 236
Query: 178 FGRIDAVKVYPEKAHAFINFVTHESAARAISQRHGYKINDNVIKCNWGKENFGFSITPLP 357
FG+I ++V+P+K ++F+ F +HESAA AI +G I +V+KC WGKE I P+
Sbjct: 237 FGQIMEIRVFPDKGYSFVRFNSHESAAHAIVSVNGTTIEGHVVKCYWGKETLDM-INPVQ 295
Query: 358 VQPAI 372
Q I
Sbjct: 296 QQNQI 300
>sp|O60176|YG41_SCHPO Hypothetical RNA-binding protein C23E6.01c in chromosome II
Length = 473
Score = 58.5 bits (140), Expect = 8e-09
Identities = 26/73 (35%), Positives = 46/73 (63%)
Frame = +1
Query: 106 NTNVYIAGIPEGLTEELVRVHFEEFGRIDAVKVYPEKAHAFINFVTHESAARAISQRHGY 285
N+ V++ G+ + ++EE ++ F+ FG I VK+ P K F+ FV +SA AI+Q GY
Sbjct: 302 NSTVFVGGLSKFVSEEELKYLFQNFGEIVYVKIPPGKGCGFVQFVNRQSAEIAINQLQGY 361
Query: 286 KINDNVIKCNWGK 324
+ ++ I+ +WG+
Sbjct: 362 PLGNSRIRLSWGR 374
>sp|P32588|PUB1_YEAST Nuclear and cytoplasmic polyadenylated RNA-binding protein PUB1
(ARS consensus binding protein ACBP-60) (Poly(U)-binding
protein) (Poly uridylate-binding protein)
Length = 453
Score = 54.7 bits (130), Expect = 1e-07
Identities = 31/92 (33%), Positives = 42/92 (45%)
Frame = +1
Query: 64 PRPYELVYKESPLTNTNVYIAGIPEGLTEELVRVHFEEFGRIDAVKVYPEKAHAFINFVT 243
P+ + + + +P T YI IP TE + F+ FG I K YPEK FI + T
Sbjct: 326 PQAVDHIIRSAPPRVTTAYIGNIPHFATEADLIPLFQNFGFILDFKHYPEKGCCFIKYDT 385
Query: 244 HESAARAISQRHGYKINDNVIKCNWGKENFGF 339
HE AA I + ++ WGKE F
Sbjct: 386 HEQAAVCIVALANFPFQGRNLRTGWGKERSNF 417
Score = 40.8 bits (94), Expect = 0.002
Identities = 18/73 (24%), Positives = 41/73 (56%), Gaps = 5/73 (6%)
Frame = +1
Query: 115 VYIAGIPEGLTEELVRVHFEEFGRIDAVKVYPEK-----AHAFINFVTHESAARAISQRH 279
+Y+ + + +TE++++ +F+ G I +K+ +K +AF+ + A A+ +
Sbjct: 77 LYVGNLDKAITEDILKQYFQVGGPIANIKIMIDKNNKNVNYAFVEYHQSHDANIALQTLN 136
Query: 280 GYKINDNVIKCNW 318
G +I +N++K NW
Sbjct: 137 GKQIENNIVKINW 149
>sp|Q00539|NAM8_YEAST NAM8 protein
Length = 523
Score = 52.4 bits (124), Expect = 6e-07
Identities = 25/73 (34%), Positives = 43/73 (58%)
Frame = +1
Query: 106 NTNVYIAGIPEGLTEELVRVHFEEFGRIDAVKVYPEKAHAFINFVTHESAARAISQRHGY 285
NT V+I G+ +TE+ +R +F+ FG I VK+ K F+ +V SA AI+ G+
Sbjct: 312 NTTVFIGGLSSLVTEDELRAYFQPFGTIVYVKIPVGKCCGFVQYVDRLSAEAAIAGMQGF 371
Query: 286 KINDNVIKCNWGK 324
I ++ ++ +WG+
Sbjct: 372 PIANSRVRLSWGR 384
>sp|Q09702|NRD1_SCHPO Negative regulator of differentiation 1 (Multicopy suppressor of
sporulation protein msa2)
Length = 529
Score = 50.8 bits (120), Expect = 2e-06
Identities = 34/114 (29%), Positives = 53/114 (46%), Gaps = 3/114 (2%)
Frame = +1
Query: 4 IKCNWATRNGLDGDQFIKYTPRPYELVYKESPLTNTNVYIAGIPEGLTEELVRVHFEEFG 183
+K W +G P P + + + N+YI + LT E ++ FEEFG
Sbjct: 389 LKIGWGKHSG----------PLPSNIALAVAGGASRNIYIGNADDSLTIERLKEDFEEFG 438
Query: 184 RIDAVKVYPEKAHAFINFVTHESAARA---ISQRHGYKINDNVIKCNWGKENFG 336
I+ V + EK AF+NF + SA A I Q+ GY+ + ++GK+ G
Sbjct: 439 EIEYVNFFREKNCAFVNFTSLASAINAIDRIKQKKGYE----NYRISYGKDRCG 488
Score = 47.8 bits (112), Expect = 1e-05
Identities = 19/54 (35%), Positives = 35/54 (64%)
Frame = +1
Query: 112 NVYIAGIPEGLTEELVRVHFEEFGRIDAVKVYPEKAHAFINFVTHESAARAISQ 273
NV++ +P G+TE+ +R E FG ID +K+ E+ AF++F+ +A +A+ +
Sbjct: 207 NVFLGNLPNGITEDEIREDLEPFGPIDQIKIVTERNIAFVHFLNIAAAIKAVQE 260
Score = 32.7 bits (73), Expect = 0.47
Identities = 28/91 (30%), Positives = 39/91 (42%), Gaps = 2/91 (2%)
Frame = +1
Query: 106 NTNVYIAGIPEGLTEELVRVHFEEFGRIDAVKVYPEKAHAFINFVTHESAAR--AISQRH 279
N VYI I T E + + G + ++ EK F+ FV SA R +S H
Sbjct: 322 NRTVYIGNIHADTTIEEI-CNAVRGGLLHNIRYLQEKHICFVTFVDPVSAFRFFEMSNIH 380
Query: 280 GYKINDNVIKCNWGKENFGFSITPLPVQPAI 372
G I + +K WGK + PLP A+
Sbjct: 381 GLVIRNRRLKIGWGKHS-----GPLPSNIAL 406
Score = 30.0 bits (66), Expect = 3.1
Identities = 23/85 (27%), Positives = 38/85 (44%), Gaps = 2/85 (2%)
Frame = +1
Query: 115 VYIAGIPEGLTEELVRVHFEEFGRIDAVKVYPEKAHAFINFV--THESAARAISQRHGYK 288
VY+ +P + + + G I++ + PEK AFI+F+ +H +A +
Sbjct: 117 VYVGNLPPNTPIDEI-LSCVRTGPIESAWILPEKNCAFISFLDPSHATAFFQDAALKRLT 175
Query: 289 INDNVIKCNWGKENFGFSITPLPVQ 363
I +K WGK + S L VQ
Sbjct: 176 IRGTEVKVGWGKNSASNSSVLLAVQ 200
>sp|P92204|NELFE_DROME Negative elongation factor E
Length = 280
Score = 47.8 bits (112), Expect = 1e-05
Identities = 23/75 (30%), Positives = 41/75 (54%)
Frame = +1
Query: 70 PYELVYKESPLTNTNVYIAGIPEGLTEELVRVHFEEFGRIDAVKVYPEKAHAFINFVTHE 249
P + E P ++++G +TE+ ++ F ++G I V + EK+ F++F E
Sbjct: 153 PMDTAQPEKPRAGNTIFVSG--NKVTEDFLKKTFNDYGTIVNVSMEIEKSRGFVSFAKPE 210
Query: 250 SAARAISQRHGYKIN 294
SA RAI++ HG +N
Sbjct: 211 SADRAIAEIHGKNVN 225
>sp|P70372|ELAV1_MOUSE ELAV-like protein 1 (Hu-antigen R) (HuR) (Elav-like generic
protein) (MelG)
Length = 326
Score = 47.4 bits (111), Expect = 2e-05
Identities = 22/78 (28%), Positives = 44/78 (56%), Gaps = 6/78 (7%)
Frame = +1
Query: 109 TNVYIAGIPEGLTEELVRVHFEEFGRIDAVKVYPEKA------HAFINFVTHESAARAIS 270
TN+ + +P+ +T+E +R F G +++ K+ +K + F+N+VT + A RAIS
Sbjct: 20 TNLIVNYLPQNMTQEELRSLFSSIGEVESAKLIRDKVAGHSLGYGFVNYVTAKDAERAIS 79
Query: 271 QRHGYKINDNVIKCNWGK 324
+G ++ IK ++ +
Sbjct: 80 TLNGLRLQSKTIKVSYAR 97
Score = 35.8 bits (81), Expect = 0.056
Identities = 22/69 (31%), Positives = 35/69 (50%), Gaps = 6/69 (8%)
Frame = +1
Query: 100 LTNTNVYIAGIPEGLTEELVRVHFEEFGRIDAVKVYPE------KAHAFINFVTHESAAR 261
+ + N+YI+G+P +T++ V F FGRI +V + + AFI F A
Sbjct: 103 IKDANLYISGLPRTMTQKDVEDMFSRFGRIINSRVLVDQTTGLSRGVAFIRFDKRSEAEE 162
Query: 262 AISQRHGYK 288
AI+ G+K
Sbjct: 163 AITSFIGHK 171
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 52,094,239
Number of Sequences: 369166
Number of extensions: 978484
Number of successful extensions: 2784
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2654
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2768
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 3020232610
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)