Planaria EST Database


DrC_01032

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01032
         (480 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P49454|CENPF_HUMAN  CENP-F kinetochore protein (Centromer...    32   0.75 
sp|Q9LME2|Y1226_ARATH  Hypothetical protein At1g22260              31   1.3  
sp|O93794|TOP2_CANGA  DNA topoisomerase 2 (DNA topoisomerase...    31   1.3  
sp|P61430|Y1275_ARATH  Hypothetical protein At1g22275              30   2.2  
sp|Q26503|TPM_SCHHA  Tropomyosin                                   30   2.2  
sp|Q26519|TPM_SCHJA  Tropomyosin                                   30   2.9  
sp|O34525|SPPA_BACSU  Putative signal peptide peptidase sppA       30   3.7  
sp|Q8XHQ5|SYC_CLOPE  Cysteinyl-tRNA synthetase (Cysteine--tR...    30   3.7  
sp|Q890M4|SYC_CLOTE  Cysteinyl-tRNA synthetase (Cysteine--tR...    30   3.7  
sp|P42637|TPM1_SCHMA  Tropomyosin 1 (TMI) (Polypeptide 49)         30   3.7  
>sp|P49454|CENPF_HUMAN CENP-F kinetochore protein (Centromere protein F) (Mitosin) (AH
           antigen)
          Length = 3210

 Score = 32.0 bits (71), Expect = 0.75
 Identities = 28/115 (24%), Positives = 55/115 (47%), Gaps = 4/115 (3%)
 Frame = +1

Query: 46  IQKREMNDECKDGMDRLYDENDKAIQKLDADCDVAFVKIVNTYTAIYETWEK-KCGLDFD 222
           I+K ++ ++C+D + R   + D+A  K  A  +    K+    +   +  E  +C L+  
Sbjct: 330 IEKEKVLNKCRDELVRTTAQYDQASTKYTA-LEQKLKKLTEDLSCQRQNAESARCSLE-- 386

Query: 223 KMSKECYEEFKVKNEAPTKEINILDKECDNAYAKLVKDFNKAEAK---LKAECSK 378
           +  KE  +EF+ +     +    LD+EC    A+L ++  +A+     L+AE  K
Sbjct: 387 QKIKEKEKEFQEELSRQQRSFQTLDQECIQMKARLTQELQQAKNMHNVLQAELDK 441
>sp|Q9LME2|Y1226_ARATH Hypothetical protein At1g22260
          Length = 871

 Score = 31.2 bits (69), Expect = 1.3
 Identities = 24/95 (25%), Positives = 41/95 (43%)
 Frame = +1

Query: 85  MDRLYDENDKAIQKLDADCDVAFVKIVNTYTAIYETWEKKCGLDFDKMSKECYEEFKVKN 264
           +  L   ND+AI ++    DV   +I+N+     E   K     FDK   +C EE K   
Sbjct: 552 LKELSQRNDQAINEIRRKYDVEKHEIINSEKDKVEKIIKDLSNKFDKELSDCKEESK--- 608

Query: 265 EAPTKEINILDKECDNAYAKLVKDFNKAEAKLKAE 369
               +++  + +E  +    L ++    E  LKA+
Sbjct: 609 ----RQLLTIQEEHSSLILSLREEHESKELNLKAK 639
>sp|O93794|TOP2_CANGA DNA topoisomerase 2 (DNA topoisomerase II)
          Length = 1406

 Score = 31.2 bits (69), Expect = 1.3
 Identities = 24/85 (28%), Positives = 41/85 (48%), Gaps = 1/85 (1%)
 Frame = +1

Query: 88   DRLYDENDKAIQKLDADCDVAFVKIVNTYTAIYETWEKKCGLDFDKMSKECYEE-FKVKN 264
            D   +E+++A +K D D +      VN    ++ T+E   GL    ++KE YE+  K K 
Sbjct: 1082 DEDLEESEEATRKKDKDDE----STVNGPEELFGTYEYLLGLKIWSLTKERYEKLLKQKQ 1137

Query: 265  EAPTKEINILDKECDNAYAKLVKDF 339
            E  T+  N+L     + +   + DF
Sbjct: 1138 EKETELENLLKLSAKDLWNNDLDDF 1162
>sp|P61430|Y1275_ARATH Hypothetical protein At1g22275
          Length = 856

 Score = 30.4 bits (67), Expect = 2.2
 Identities = 22/95 (23%), Positives = 42/95 (44%)
 Frame = +1

Query: 85  MDRLYDENDKAIQKLDADCDVAFVKIVNTYTAIYETWEKKCGLDFDKMSKECYEEFKVKN 264
           +  L   ND+AI ++    DV   +I+N+     E   K+    +DK   +C EE K   
Sbjct: 552 LKELSQRNDQAINEIRRKYDVEKHEIINSEKDKVEKIIKELSTKYDKGLSDCKEESK--- 608

Query: 265 EAPTKEINILDKECDNAYAKLVKDFNKAEAKLKAE 369
               +++  + +E  +    + ++    E  LKA+
Sbjct: 609 ----RQLLTIQEEHSSRILNIREEHESKELNLKAK 639
>sp|Q26503|TPM_SCHHA Tropomyosin
          Length = 284

 Score = 30.4 bits (67), Expect = 2.2
 Identities = 33/153 (21%), Positives = 69/153 (45%), Gaps = 30/153 (19%)
 Frame = +1

Query: 16  ECRYRMRKNEIQKREMNDECKDGMDRLYDENDKA-------------IQKLDADCDVAFV 156
           E   +M++ +I   E+ +  ++ M++L + + +A             I+ L+ D +V+  
Sbjct: 45  ELNTKMKQAQIDCDEVQETLQEQMNKLEETDKRATNAEAEVAAMTRRIRLLEEDLEVSSS 104

Query: 157 KIVNTYTAIYETWEKKCGLDFDKMSKECYEEFKVKNEAPTKEINIL-------------- 294
           ++  T T + E  +        + S+   ++ ++++ A  + +N L              
Sbjct: 105 RLTETLTKLEEASKTA------EESERGRKDLEIRSIADDERLNQLEDQQKEAKYIAEDA 158

Query: 295 DKECDNAYAKLV---KDFNKAEAKLKAECSKSV 384
           D++ D A  KL     DF +AEA+L+A  SK V
Sbjct: 159 DRKYDEAARKLAIAEVDFKRAEARLEAAESKIV 191
>sp|Q26519|TPM_SCHJA Tropomyosin
          Length = 284

 Score = 30.0 bits (66), Expect = 2.9
 Identities = 33/153 (21%), Positives = 68/153 (44%), Gaps = 30/153 (19%)
 Frame = +1

Query: 16  ECRYRMRKNEIQKREMNDECKDGMDRLYDENDKA-------------IQKLDADCDVAFV 156
           E   +M++ +I   E+ +  ++ M++L +   +A             I+ L+ D +V+  
Sbjct: 45  ELNTKMKQAQIDCDEVQETLQEQMNKLEETEKRATNAEAQVAAMTRRIRLLEEDLEVSSS 104

Query: 157 KIVNTYTAIYETWEKKCGLDFDKMSKECYEEFKVKNEAPTKEINIL-------------- 294
           ++  T T + E  +        + S+   ++ ++++ A  + +N L              
Sbjct: 105 RLTETLTKLEEASKTA------EESERGRKDLEIRSIADDERLNQLEDQQKEAKYIAEDA 158

Query: 295 DKECDNAYAKLV---KDFNKAEAKLKAECSKSV 384
           D++ D A  KL     DF +AEA+L+A  SK V
Sbjct: 159 DRKYDEAARKLAIAEVDFERAEARLEAAESKIV 191
>sp|O34525|SPPA_BACSU Putative signal peptide peptidase sppA
          Length = 335

 Score = 29.6 bits (65), Expect = 3.7
 Identities = 18/66 (27%), Positives = 28/66 (42%), Gaps = 1/66 (1%)
 Frame = +1

Query: 184 YETWEKKCGLDFDKMSKECYEEFKVKNEAPTKEI-NILDKECDNAYAKLVKDFNKAEAKL 360
           Y     K G+ F+ +    +++    +   TKE  NI+    DN+Y   V   +K     
Sbjct: 179 YSKLADKLGISFETIKSGAHKDIMSPSREMTKEEKNIMQSMVDNSYEGFVDVISKGRGMP 238

Query: 361 KAECSK 378
           KAE  K
Sbjct: 239 KAEVKK 244
>sp|Q8XHQ5|SYC_CLOPE Cysteinyl-tRNA synthetase (Cysteine--tRNA ligase) (CysRS)
          Length = 466

 Score = 29.6 bits (65), Expect = 3.7
 Identities = 16/50 (32%), Positives = 29/50 (58%)
 Frame = +1

Query: 40  NEIQKREMNDECKDGMDRLYDENDKAIQKLDADCDVAFVKIVNTYTAIYE 189
           +E+ + EMN+E K  ++ L    +K I+K+D D + A     +  TAI++
Sbjct: 330 DEVSREEMNEEEKAYLESLNKYKEKYIEKMDDDFNTA-----DAITAIFD 374
>sp|Q890M4|SYC_CLOTE Cysteinyl-tRNA synthetase (Cysteine--tRNA ligase) (CysRS)
          Length = 465

 Score = 29.6 bits (65), Expect = 3.7
 Identities = 12/37 (32%), Positives = 25/37 (67%)
 Frame = +1

Query: 40  NEIQKREMNDECKDGMDRLYDENDKAIQKLDADCDVA 150
           NE+++ ++N+E K  + +L +  +K I+K+D D + A
Sbjct: 329 NEVKRNDLNEEEKKYIKKLDEYREKYIEKMDDDFNTA 365
>sp|P42637|TPM1_SCHMA Tropomyosin 1 (TMI) (Polypeptide 49)
          Length = 284

 Score = 29.6 bits (65), Expect = 3.7
 Identities = 33/153 (21%), Positives = 68/153 (44%), Gaps = 30/153 (19%)
 Frame = +1

Query: 16  ECRYRMRKNEIQKREMNDECKDGMDRLYDENDKA-------------IQKLDADCDVAFV 156
           E   +M++ +I   E  +  ++ M++L + + +A             I+ L+ D +V+  
Sbjct: 45  ELNNKMKQAQIDCDEAQETLQEQMNKLEETDKRATNAEAEVAAMTRRIRLLEEDLEVSSS 104

Query: 157 KIVNTYTAIYETWEKKCGLDFDKMSKECYEEFKVKNEAPTKEINIL-------------- 294
           ++  T T + E  +        + S+   ++ ++++ A  + +N L              
Sbjct: 105 RLTETLTKLEEASKTA------EESERGRKDLEIRSIADDERLNQLEDQQKEAKYIAEDA 158

Query: 295 DKECDNAYAKLV---KDFNKAEAKLKAECSKSV 384
           D++ D A  KL     DF +AEA+L+A  SK V
Sbjct: 159 DRKYDEAARKLAIAEVDFERAEARLEAAESKIV 191
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 40,924,871
Number of Sequences: 369166
Number of extensions: 666145
Number of successful extensions: 2365
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2291
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2364
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 2822184570
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_01032

  1. Dr_sW_026_B12
  2. Dr_sW_001_O20