Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_026_B12
(417 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O93794|TOP2_CANGA DNA topoisomerase 2 (DNA topoisomerase... 33 0.23
sp|P49454|CENPF_HUMAN CENP-F kinetochore protein (Centromer... 32 0.52
sp|Q26503|TPM_SCHHA Tropomyosin 31 0.89
sp|Q9LME2|Y1226_ARATH Hypothetical protein At1g22260 31 1.2
sp|Q26519|TPM_SCHJA Tropomyosin 31 1.2
sp|P61430|Y1275_ARATH Hypothetical protein At1g22275 30 1.5
sp|P42637|TPM1_SCHMA Tropomyosin 1 (TMI) (Polypeptide 49) 30 1.5
sp|P16952|SSP5_STRGN Agglutinin receptor precursor (SSP-5) 30 1.5
sp|Q8XHQ5|SYC_CLOPE Cysteinyl-tRNA synthetase (Cysteine--tR... 30 2.6
sp|Q890M4|SYC_CLOTE Cysteinyl-tRNA synthetase (Cysteine--tR... 30 2.6
>sp|O93794|TOP2_CANGA DNA topoisomerase 2 (DNA topoisomerase II)
Length = 1406
Score = 33.1 bits (74), Expect = 0.23
Identities = 25/85 (29%), Positives = 41/85 (48%), Gaps = 1/85 (1%)
Frame = +1
Query: 88 DRLYDENDKAIQKLDADCDVAFVKIVNTYTAIYETWEKKCGLDFDKMSKECYEE-FKVKN 264
D +E+++A +K D D + VN ++ T+E GL ++KE YE+ K K
Sbjct: 1082 DEDLEESEEATRKKDKDDE----STVNGPEELFGTYEYLLGLKIWSLTKERYEKLLKQKQ 1137
Query: 265 EAPTKEINILDKECDDAYAKLVKDF 339
E T+ N+L D + + DF
Sbjct: 1138 EKETELENLLKLSAKDLWNNDLDDF 1162
>sp|P49454|CENPF_HUMAN CENP-F kinetochore protein (Centromere protein F) (Mitosin) (AH
antigen)
Length = 3210
Score = 32.0 bits (71), Expect = 0.52
Identities = 28/115 (24%), Positives = 55/115 (47%), Gaps = 4/115 (3%)
Frame = +1
Query: 46 IQKREMNDECKDGMDRLYDENDKAIQKLDADCDVAFVKIVNTYTAIYETWEK-KCGLDFD 222
I+K ++ ++C+D + R + D+A K A + K+ + + E +C L+
Sbjct: 330 IEKEKVLNKCRDELVRTTAQYDQASTKYTA-LEQKLKKLTEDLSCQRQNAESARCSLE-- 386
Query: 223 KMSKECYEEFKVKNEAPTKEINILDKECDDAYAKLVKDFNKAEAK---LKAECSK 378
+ KE +EF+ + + LD+EC A+L ++ +A+ L+AE K
Sbjct: 387 QKIKEKEKEFQEELSRQQRSFQTLDQECIQMKARLTQELQQAKNMHNVLQAELDK 441
>sp|Q26503|TPM_SCHHA Tropomyosin
Length = 284
Score = 31.2 bits (69), Expect = 0.89
Identities = 33/153 (21%), Positives = 70/153 (45%), Gaps = 30/153 (19%)
Frame = +1
Query: 16 ECRYRMRKNEIQKREMNDECKDGMDRLYDENDKA-------------IQKLDADCDVAFV 156
E +M++ +I E+ + ++ M++L + + +A I+ L+ D +V+
Sbjct: 45 ELNTKMKQAQIDCDEVQETLQEQMNKLEETDKRATNAEAEVAAMTRRIRLLEEDLEVSSS 104
Query: 157 KIVNTYTAIYETWEKKCGLDFDKMSKECYEEFKVKNEAPTKEINIL-------------- 294
++ T T + E + + S+ ++ ++++ A + +N L
Sbjct: 105 RLTETLTKLEEASKTA------EESERGRKDLEIRSIADDERLNQLEDQQKEAKYIAEDA 158
Query: 295 DKECDDAYAKLV---KDFNKAEAKLKAECSKSV 384
D++ D+A KL DF +AEA+L+A SK V
Sbjct: 159 DRKYDEAARKLAIAEVDFKRAEARLEAAESKIV 191
>sp|Q9LME2|Y1226_ARATH Hypothetical protein At1g22260
Length = 871
Score = 30.8 bits (68), Expect = 1.2
Identities = 24/95 (25%), Positives = 40/95 (42%)
Frame = +1
Query: 85 MDRLYDENDKAIQKLDADCDVAFVKIVNTYTAIYETWEKKCGLDFDKMSKECYEEFKVKN 264
+ L ND+AI ++ DV +I+N+ E K FDK +C EE K
Sbjct: 552 LKELSQRNDQAINEIRRKYDVEKHEIINSEKDKVEKIIKDLSNKFDKELSDCKEESK--- 608
Query: 265 EAPTKEINILDKECDDAYAKLVKDFNKAEAKLKAE 369
+++ + +E L ++ E LKA+
Sbjct: 609 ----RQLLTIQEEHSSLILSLREEHESKELNLKAK 639
>sp|Q26519|TPM_SCHJA Tropomyosin
Length = 284
Score = 30.8 bits (68), Expect = 1.2
Identities = 33/153 (21%), Positives = 69/153 (45%), Gaps = 30/153 (19%)
Frame = +1
Query: 16 ECRYRMRKNEIQKREMNDECKDGMDRLYDENDKA-------------IQKLDADCDVAFV 156
E +M++ +I E+ + ++ M++L + +A I+ L+ D +V+
Sbjct: 45 ELNTKMKQAQIDCDEVQETLQEQMNKLEETEKRATNAEAQVAAMTRRIRLLEEDLEVSSS 104
Query: 157 KIVNTYTAIYETWEKKCGLDFDKMSKECYEEFKVKNEAPTKEINIL-------------- 294
++ T T + E + + S+ ++ ++++ A + +N L
Sbjct: 105 RLTETLTKLEEASKTA------EESERGRKDLEIRSIADDERLNQLEDQQKEAKYIAEDA 158
Query: 295 DKECDDAYAKLV---KDFNKAEAKLKAECSKSV 384
D++ D+A KL DF +AEA+L+A SK V
Sbjct: 159 DRKYDEAARKLAIAEVDFERAEARLEAAESKIV 191
>sp|P61430|Y1275_ARATH Hypothetical protein At1g22275
Length = 856
Score = 30.4 bits (67), Expect = 1.5
Identities = 24/110 (21%), Positives = 46/110 (41%), Gaps = 16/110 (14%)
Frame = +1
Query: 85 MDRLYDENDKAIQKLDADCDVAFVKIVNTYTAIYETWEKKCGLDFDKMSKECYEEFK--- 255
+ L ND+AI ++ DV +I+N+ E K+ +DK +C EE K
Sbjct: 552 LKELSQRNDQAINEIRRKYDVEKHEIINSEKDKVEKIIKELSTKYDKGLSDCKEESKRQL 611
Query: 256 -------------VKNEAPTKEINILDKECDDAYAKLVKDFNKAEAKLKA 366
++ E +KE+N+ K + ++ N+ + ++ A
Sbjct: 612 LTIQEEHSSRILNIREEHESKELNLKAKYDQELRQNQIQAENELKERITA 661
>sp|P42637|TPM1_SCHMA Tropomyosin 1 (TMI) (Polypeptide 49)
Length = 284
Score = 30.4 bits (67), Expect = 1.5
Identities = 33/153 (21%), Positives = 69/153 (45%), Gaps = 30/153 (19%)
Frame = +1
Query: 16 ECRYRMRKNEIQKREMNDECKDGMDRLYDENDKA-------------IQKLDADCDVAFV 156
E +M++ +I E + ++ M++L + + +A I+ L+ D +V+
Sbjct: 45 ELNNKMKQAQIDCDEAQETLQEQMNKLEETDKRATNAEAEVAAMTRRIRLLEEDLEVSSS 104
Query: 157 KIVNTYTAIYETWEKKCGLDFDKMSKECYEEFKVKNEAPTKEINIL-------------- 294
++ T T + E + + S+ ++ ++++ A + +N L
Sbjct: 105 RLTETLTKLEEASKTA------EESERGRKDLEIRSIADDERLNQLEDQQKEAKYIAEDA 158
Query: 295 DKECDDAYAKLV---KDFNKAEAKLKAECSKSV 384
D++ D+A KL DF +AEA+L+A SK V
Sbjct: 159 DRKYDEAARKLAIAEVDFERAEARLEAAESKIV 191
>sp|P16952|SSP5_STRGN Agglutinin receptor precursor (SSP-5)
Length = 1500
Score = 30.4 bits (67), Expect = 1.5
Identities = 30/106 (28%), Positives = 44/106 (41%), Gaps = 22/106 (20%)
Frame = +1
Query: 118 IQKLDADCDVAFVKIVN-------TYTAIYETWEKKCGLDFD--------KMSKECYEEF 252
+ K AD A VK+V T T + +K+ + D K + E Y++
Sbjct: 96 LDKAVADAKSAGVKVVQDETKDKGTATTATDNAQKQDEIKSDYAKQAEEIKTTTEAYKKE 155
Query: 253 KVKNEAPTKEINILDKECDDAYAKLVKD-------FNKAEAKLKAE 369
++A T +IN +K DD Y K +K N A A KAE
Sbjct: 156 VAAHQAETDKINAENKAADDKYQKDLKSHQEEVEKINTANATAKAE 201
>sp|Q8XHQ5|SYC_CLOPE Cysteinyl-tRNA synthetase (Cysteine--tRNA ligase) (CysRS)
Length = 466
Score = 29.6 bits (65), Expect = 2.6
Identities = 16/50 (32%), Positives = 29/50 (58%)
Frame = +1
Query: 40 NEIQKREMNDECKDGMDRLYDENDKAIQKLDADCDVAFVKIVNTYTAIYE 189
+E+ + EMN+E K ++ L +K I+K+D D + A + TAI++
Sbjct: 330 DEVSREEMNEEEKAYLESLNKYKEKYIEKMDDDFNTA-----DAITAIFD 374
>sp|Q890M4|SYC_CLOTE Cysteinyl-tRNA synthetase (Cysteine--tRNA ligase) (CysRS)
Length = 465
Score = 29.6 bits (65), Expect = 2.6
Identities = 12/37 (32%), Positives = 25/37 (67%)
Frame = +1
Query: 40 NEIQKREMNDECKDGMDRLYDENDKAIQKLDADCDVA 150
NE+++ ++N+E K + +L + +K I+K+D D + A
Sbjct: 329 NEVKRNDLNEEEKKYIKKLDEYREKYIEKMDDDFNTA 365
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 36,861,822
Number of Sequences: 369166
Number of extensions: 610830
Number of successful extensions: 2257
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2178
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2256
length of database: 68,354,980
effective HSP length: 99
effective length of database: 50,066,215
effective search space used: 1952582385
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)