Planaria EST Database

DrC_00552

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00552
         (514 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q62217|SEM5A_MOUSE  Semaphorin-5A precursor (Semaphorin F...    30   2.6  
sp|Q7ZZV4|TSHB_ANGJA  Thyrotropin beta chain precursor (Thyr...    30   2.6  
sp|P98133|FBN1_BOVIN  Fibrillin-1 precursor (MP340)                30   3.3  
sp|Q92HI8|PPDK_RICCN  Pyruvate, phosphate dikinase (Pyruvate...    30   3.3  
sp|Q08127|TSHB_ANGAN  Thyrotropin beta chain precursor (Thyr...    30   4.4  
sp|P35555|FBN1_HUMAN  Fibrillin-1 precursor                        29   5.7  
sp|Q09272|YQG1_CAEEL  Hypothetical protein C41C4.1 in chromo...    29   5.7  
sp|P10820|PERF_MOUSE  Perforin 1 precursor (P1) (Lymphocyte ...    29   7.5  
sp|Q01253|YSCL_YEREN  Yop proteins translocation protein L         29   7.5  
sp|Q13591|SEM5A_HUMAN  Semaphorin-5A precursor (Semaphorin F...    29   7.5  

>sp|Q62217|SEM5A_MOUSE Semaphorin-5A precursor (Semaphorin F) (Sema F)
          Length = 1077

 Score = 30.4 bits (67), Expect = 2.6
 Identities = 30/124 (24%), Positives = 47/124 (37%), Gaps = 18/124 (14%)
 Frame = +1

Query: 112  RCRWCSQAYPDRFQCENCVQTNNNCCFDPRNINIVSPALSICRQQRSCTFINLSXXXXXX 291
            R R C   +P   QC      +  C FD   I  VS A S   +++ C   N+       
Sbjct: 916  RARQCILLFPVGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMFHMM 975

Query: 292  XXG-----FGCINCVLNFQNC-----------CVSPINP--LNNYIAQNFCNFIDDQQ*L 417
              G      GC+  +L +  C            + P++P  LN+ I  N  N +D    +
Sbjct: 976  AVGLSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAALNSSIT-NHINKLDKYDSV 1034

Query: 418  DFLK 429
            + +K
Sbjct: 1035 EAIK 1038

>sp|Q7ZZV4|TSHB_ANGJA Thyrotropin beta chain precursor (Thyroid-stimulating hormone beta
           subunit) (TSH-beta) (TSH-B)
          Length = 147

 Score = 30.4 bits (67), Expect = 2.6
 Identities = 16/48 (33%), Positives = 23/48 (47%), Gaps = 6/48 (12%)
 Frame = +1

Query: 136 YPDRFQCENCVQTNNNCCF------DPRNINIVSPALSICRQQRSCTF 261
           Y ++ +C+ CV  N   C       DP   N+V PA+     QR CT+
Sbjct: 30  YVEKPECDFCVAINTTICMGFCYSLDP---NVVGPAVKRLAVQRGCTY 74

>sp|P98133|FBN1_BOVIN Fibrillin-1 precursor (MP340)
          Length = 2871

 Score = 30.0 bits (66), Expect = 3.3
 Identities = 29/114 (25%), Positives = 42/114 (36%), Gaps = 11/114 (9%)
 Frame = +1

Query: 34   ACVEISETQLPETQNRFQQCPNYSMSRCRWCSQAY---PDRFQC---ENCVQTNNNC--- 186
            AC++I+E +L        +C N        C+  Y   PDR  C   + C   N  C   
Sbjct: 1152 ACIDINECELSAHLCPHGRCVNLIGKYQCACNPGYHSTPDRLFCVDIDECSIMNGGCETF 1211

Query: 187  CFDPRNINIVS--PALSICRQQRSCTFINLSXXXXXXXXGFGCINCVLNFQNCC 342
            C +       S  P  ++   QRSCT I+          G  C N    ++  C
Sbjct: 1212 CTNSEGSYECSCQPGFALMPDQRSCTDIDECEDNPNICDGGQCTNIPGEYRCLC 1265

>sp|Q92HI8|PPDK_RICCN Pyruvate, phosphate dikinase (Pyruvate, orthophosphate dikinase)
          Length = 882

 Score = 30.0 bits (66), Expect = 3.3
 Identities = 22/77 (28%), Positives = 38/77 (49%), Gaps = 1/77 (1%)
 Frame = +2

Query: 38  VWKSVKRNCQKLKIDFNNAQTIQCLVADGVLKLIQTDFNVKIAFKLITIAV-LIHETLIL 214
           V+K + +  QKL+  + + Q I+  + +  L ++QT    + A   I IAV ++ E LI 
Sbjct: 312 VFKELSQIAQKLEEHYLDMQDIEFTIENNKLYILQTRTAKRTAIAAINIAVQMVEEKLIS 371

Query: 215 CHQLCQYVDNKEAVLLL 265
             Q    +D +    LL
Sbjct: 372 KEQALMRIDPESLNQLL 388

>sp|Q08127|TSHB_ANGAN Thyrotropin beta chain precursor (Thyroid-stimulating hormone beta
           subunit) (TSH-beta) (TSH-B)
          Length = 147

 Score = 29.6 bits (65), Expect = 4.4
 Identities = 16/48 (33%), Positives = 23/48 (47%), Gaps = 6/48 (12%)
 Frame = +1

Query: 136 YPDRFQCENCVQTNNNCCF------DPRNINIVSPALSICRQQRSCTF 261
           Y ++ +C+ CV  N   C       DP   N+V PA+     QR CT+
Sbjct: 30  YVEKPECDFCVAINTTICMGFCYSLDP---NVVGPAVKRLVVQRGCTY 74

>sp|P35555|FBN1_HUMAN Fibrillin-1 precursor
          Length = 2871

 Score = 29.3 bits (64), Expect = 5.7
 Identities = 29/114 (25%), Positives = 42/114 (36%), Gaps = 11/114 (9%)
 Frame = +1

Query: 34   ACVEISETQLPETQNRFQQCPNYSMSRCRWCSQAY---PDRFQC---ENCVQTNNNC--- 186
            AC++I+E +L        +C N        C+  Y   PDR  C   + C   N  C   
Sbjct: 1152 ACIDINECELSAHLCPNGRCVNLIGKYQCACNPGYHSTPDRLFCVDIDECSIMNGGCETF 1211

Query: 187  CFDPRNINIVS--PALSICRQQRSCTFINLSXXXXXXXXGFGCINCVLNFQNCC 342
            C +       S  P  ++   QRSCT I+          G  C N    ++  C
Sbjct: 1212 CTNSEGSYECSCQPGFALMPDQRSCTDIDECEDNPNICDGGQCTNIPGEYRCLC 1265

>sp|Q09272|YQG1_CAEEL Hypothetical protein C41C4.1 in chromosome II
          Length = 329

 Score = 29.3 bits (64), Expect = 5.7
 Identities = 13/30 (43%), Positives = 19/30 (63%)
 Frame = +1

Query: 97  NYSMSRCRWCSQAYPDRFQCENCVQTNNNC 186
           ++  ++C + +Q YPDR QC NC Q N  C
Sbjct: 165 SFFSNKCIFEAQKYPDRKQCYNC-QGNVTC 193

>sp|P10820|PERF_MOUSE Perforin 1 precursor (P1) (Lymphocyte pore forming protein)
           (Cytolysin)
          Length = 554

 Score = 28.9 bits (63), Expect = 7.5
 Identities = 17/58 (29%), Positives = 26/58 (44%), Gaps = 12/58 (20%)
 Frame = +1

Query: 52  ETQLPETQNRFQQCPNYSMSRCRWCSQAYPDR------------FQCENCVQTNNNCC 189
           E Q P+ +   Q   +Y MSR RW + + P R             +C++   TN +CC
Sbjct: 350 EEQNPKREALRQAISHYIMSRARWQNCSRPCRSGQHKSSHDSCQCECQDSKVTNQDCC 407

>sp|Q01253|YSCL_YEREN Yop proteins translocation protein L
          Length = 223

 Score = 28.9 bits (63), Expect = 7.5
 Identities = 16/42 (38%), Positives = 23/42 (54%), Gaps = 2/42 (4%)
 Frame = +2

Query: 185 AVLIHETLILCHQLCQYVDNK--EAVLLLTCHNVKDVDNRDL 304
           A LIHET + C Q  ++V+ +  E VLL     + D D  D+
Sbjct: 85  ATLIHETQLQCQQFYRHVEQQMSEVVLLAVRKILNDYDQVDM 126

>sp|Q13591|SEM5A_HUMAN Semaphorin-5A precursor (Semaphorin F) (Sema F)
          Length = 1074

 Score = 28.9 bits (63), Expect = 7.5
 Identities = 29/121 (23%), Positives = 45/121 (37%), Gaps = 15/121 (12%)
 Frame = +1

Query: 112  RCRWCSQAYPDRFQCENCVQTNNNCCFDPRNINIVSPALSICRQQRSCTFINLSXXXXXX 291
            R R C   +P   QC      +  C FD   I  VS A S   +++ C   N+       
Sbjct: 916  RARQCILLFPMGSQCSGNTTESRPCVFDSNFIPEVSVARSSSVEEKRCGEFNMFHMIAVG 975

Query: 292  XXG--FGCINCVLNFQNC-----------CVSPIN--PLNNYIAQNFCNFIDDQQ*LDFL 426
                  GC+  +L +  C            + P++  PLN  I  N  N +D    ++ +
Sbjct: 976  LSSSILGCLLTLLVYTYCQRYQQQSHDATVIHPVSPAPLNTSIT-NHINKLDKYDSVEAI 1034

Query: 427  K 429
            K
Sbjct: 1035 K 1035

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 55,561,226
Number of Sequences: 369166
Number of extensions: 1032623
Number of successful extensions: 2283
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2210
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2282
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3304927425
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00552

  1. Dr_sW_014_F07
  2. Dr_sW_019_B01
  3. Dr_sW_024_J11
  4. Dr_sW_020_H10