Planaria EST Database

DrC_00549

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00549
         (625 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P24032|MLRN_CHICK  Myosin regulatory light chain 2, smoot...   223   3e-58
sp|P19105|MLRM_HUMAN  Myosin regulatory light chain 2, nonsa...   223   4e-58
sp|P18666|MLRB_RAT  Myosin regulatory light chain 2-B, smoot...   222   7e-58
sp|Q09510|MLRH_CAEEL  Probable myosin regulatory light chain      222   7e-58
sp|Q9CQ19|MLRN_MOUSE  Myosin regulatory light chain 2, smoot...   221   9e-58
sp|P29269|MLRN_PIG  Myosin regulatory light chain 2, smooth ...   221   9e-58
sp|P24844|MLRN_HUMAN  Myosin regulatory light chain 2, smoot...   221   9e-58
sp|P02612|MLRM_CHICK  Myosin regulatory light chain 2, smoot...   221   2e-57
sp|P13832|MLRA_RAT  Myosin regulatory light chain 2-A, smoot...   217   2e-56
sp|Q64122|MLRN_RAT  Myosin regulatory light chain 2, smooth ...   212   5e-55

>sp|P24032|MLRN_CHICK Myosin regulatory light chain 2, smooth muscle minor isoform (G1)
           (DTNB) (MLC-2) (Isoform L20-B1)
          Length = 172

 Score =  223 bits (568), Expect = 3e-58
 Identities = 107/169 (63%), Positives = 136/169 (80%), Gaps = 1/169 (0%)
 Frame = +2

Query: 41  ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLG 220
           A TKT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLG
Sbjct: 6   AKTKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLG 62

Query: 221 KDANEAYIEEMLKQAAGTINFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVSEEH 400
           K+  + Y++ M+ +A G INFTMFLT+FGEK+ G+DPED I NAFACFD + TGF+ E++
Sbjct: 63  KNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGFIQEDY 122

Query: 401 LRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 544
           LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct: 123 LRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171

>sp|P19105|MLRM_HUMAN Myosin regulatory light chain 2, nonsarcomeric (Myosin RLC)
 sp|Q5RC34|MLRM_PONPY Myosin regulatory light chain 2, nonsarcomeric (Myosin RLC)
          Length = 171

 Score =  223 bits (567), Expect = 4e-58
 Identities = 104/170 (61%), Positives = 137/170 (80%), Gaps = 1/170 (0%)
 Frame = +2

Query: 38  MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSL 217
           M+S +T+ K + R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SL
Sbjct: 1   MSSKRTKTKTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASL 60

Query: 218 GKDANEAYIEEMLKQAAGTINFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVSEE 397
           GK+  + Y++ M+ +A G INFTMFLT+FGEK+ G+DPED I NAFACFD + TG + E+
Sbjct: 61  GKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQED 120

Query: 398 HLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 544
           +LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct: 121 YLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 170

>sp|P18666|MLRB_RAT Myosin regulatory light chain 2-B, smooth muscle isoform (Myosin
           RLC-B)
          Length = 172

 Score =  222 bits (565), Expect = 7e-58
 Identities = 107/169 (63%), Positives = 136/169 (80%), Gaps = 1/169 (0%)
 Frame = +2

Query: 41  ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLG 220
           A TKT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLG
Sbjct: 6   AKTKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLG 62

Query: 221 KDANEAYIEEMLKQAAGTINFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVSEEH 400
           K+  +AY++ M+ +A G INFTMFLT+FGEK+ G+DPED I NAFACFD + TG + E++
Sbjct: 63  KNPTDAYLDAMMNEAPGRINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDY 122

Query: 401 LRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 544
           LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct: 123 LRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171

>sp|Q09510|MLRH_CAEEL Probable myosin regulatory light chain
          Length = 172

 Score =  222 bits (565), Expect = 7e-58
 Identities = 107/171 (62%), Positives = 138/171 (80%), Gaps = 2/171 (1%)
 Frame = +2

Query: 38  MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSL 217
           MAS KT  + R R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +M  SL
Sbjct: 1   MASRKTVNR-RQRPQRATSNVFAMFDQAQIQEFKEAFNMIDQNRDGFIDQEDLKDMFASL 59

Query: 218 GKDANEAYIEEMLKQAAGT--INFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVS 391
           GK+  E +I+ M+ +A G   INFTMFLTLFGEK+TG+DPE+ I NAF CFD D++G ++
Sbjct: 60  GKEVTEQFIDSMINEAPGAQPINFTMFLTLFGEKLTGTDPEEVIRNAFQCFDEDNSGKLN 119

Query: 392 EEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 544
           EEHLR+L+T+MG+R+++EQVDELF  API  G+F+Y EFT+M+KHG K++D
Sbjct: 120 EEHLRELLTTMGERYSEEQVDELFRDAPIKGGQFDYVEFTRMLKHGTKDKD 170

>sp|Q9CQ19|MLRN_MOUSE Myosin regulatory light chain 2, smooth muscle isoform (Myosin RLC)
          Length = 172

 Score =  221 bits (564), Expect = 9e-58
 Identities = 106/169 (62%), Positives = 135/169 (79%), Gaps = 1/169 (0%)
 Frame = +2

Query: 41  ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLG 220
           A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLG
Sbjct: 6   AKAKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLG 62

Query: 221 KDANEAYIEEMLKQAAGTINFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVSEEH 400
           K+  + Y+E M+ +A G INFTMFLT+FGEK+ G+DPED I NAFACFD + +GF+ E+H
Sbjct: 63  KNPTDEYLEGMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDH 122

Query: 401 LRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 544
           LR+L+T+MGDR+TDE+VDE++  API   G FNY EFT+++KHG K++D
Sbjct: 123 LRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKD 171

>sp|P29269|MLRN_PIG Myosin regulatory light chain 2, smooth muscle isoform (Myosin RLC)
           (LC20)
          Length = 171

 Score =  221 bits (564), Expect = 9e-58
 Identities = 106/169 (62%), Positives = 135/169 (79%), Gaps = 1/169 (0%)
 Frame = +2

Query: 41  ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLG 220
           A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLG
Sbjct: 5   AKAKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLG 61

Query: 221 KDANEAYIEEMLKQAAGTINFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVSEEH 400
           K+  + Y+E M+ +A G INFTMFLT+FGEK+ G+DPED I NAFACFD + +GF+ E+H
Sbjct: 62  KNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDH 121

Query: 401 LRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 544
           LR+L+T+MGDR+TDE+VDE++  API   G FNY EFT+++KHG K++D
Sbjct: 122 LRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKD 170

>sp|P24844|MLRN_HUMAN Myosin regulatory light chain 2, smooth muscle isoform (Myosin RLC)
           (LC20)
          Length = 172

 Score =  221 bits (564), Expect = 9e-58
 Identities = 106/169 (62%), Positives = 135/169 (79%), Gaps = 1/169 (0%)
 Frame = +2

Query: 41  ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLG 220
           A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLG
Sbjct: 6   AKAKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLG 62

Query: 221 KDANEAYIEEMLKQAAGTINFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVSEEH 400
           K+  + Y+E M+ +A G INFTMFLT+FGEK+ G+DPED I NAFACFD + +GF+ E+H
Sbjct: 63  KNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDH 122

Query: 401 LRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 544
           LR+L+T+MGDR+TDE+VDE++  API   G FNY EFT+++KHG K++D
Sbjct: 123 LRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKD 171

>sp|P02612|MLRM_CHICK Myosin regulatory light chain 2, smooth muscle major isoform (G1)
           (DTNB) (MLC-2) (Isoform L20-A)
          Length = 172

 Score =  221 bits (562), Expect = 2e-57
 Identities = 105/169 (62%), Positives = 135/169 (79%), Gaps = 1/169 (0%)
 Frame = +2

Query: 41  ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLG 220
           A  KT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML S+G
Sbjct: 6   AKAKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASMG 62

Query: 221 KDANEAYIEEMLKQAAGTINFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVSEEH 400
           K+  + Y+E M+ +A G INFTMFLT+FGEK+ G+DPED I NAFACFD + +GF+ E+H
Sbjct: 63  KNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDH 122

Query: 401 LRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 544
           LR+L+T+MGDR+TDE+VDE++  API   G FNY EFT+++KHG K++D
Sbjct: 123 LRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKD 171

>sp|P13832|MLRA_RAT Myosin regulatory light chain 2-A, smooth muscle isoform (Myosin
           RLC-A)
          Length = 172

 Score =  217 bits (552), Expect = 2e-56
 Identities = 104/169 (61%), Positives = 134/169 (79%), Gaps = 1/169 (0%)
 Frame = +2

Query: 41  ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLG 220
           A TKT KK   R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML S+G
Sbjct: 6   AKTKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASMG 62

Query: 221 KDANEAYIEEMLKQAAGTINFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVSEEH 400
           K+  + Y++ M+ +A G INFTMFLT+FGEK+ G+DPED I NAFACFD +  G + E++
Sbjct: 63  KNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEAIGTIQEDY 122

Query: 401 LRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 544
           LR+L+T+MGDR+TDE+VDEL+  API   G FNY EFT+++KHG K++D
Sbjct: 123 LRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171

>sp|Q64122|MLRN_RAT Myosin regulatory light chain 2, smooth muscle isoform (Myosin RLC)
          Length = 171

 Score =  212 bits (540), Expect = 5e-55
 Identities = 101/168 (60%), Positives = 130/168 (77%)
 Frame = +2

Query: 41  ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLG 220
           A  KT KK   R Q  TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLG
Sbjct: 6   AKAKTTKK---RPQSATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLG 62

Query: 221 KDANEAYIEEMLKQAAGTINFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVSEEH 400
           K+  +  +E M+ +A G INFTMFLT+FGEK+ G+DPED I NAFACFD + +GF+ E+H
Sbjct: 63  KNPTDEXLEGMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDH 122

Query: 401 LRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 544
           LR+L+T+MGDR+TDE+VDE++       G FNY EFT+++KHG K++D
Sbjct: 123 LRELLTTMGDRFTDEEVDEMYRERIDKKGNFNYVEFTRILKHGAKDKD 170

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 63,305,225
Number of Sequences: 369166
Number of extensions: 1213129
Number of successful extensions: 4332
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3763
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4005
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 4926080070
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00549

  1. Dr_sW_014_E04
  2. Dr_sW_023_M12