Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_023_M12
(624 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P24032|MLRN_CHICK Myosin regulatory light chain 2, smoot... 223 3e-58
sp|P19105|MLRM_HUMAN Myosin regulatory light chain 2, nonsa... 223 4e-58
sp|P18666|MLRB_RAT Myosin regulatory light chain 2-B, smoot... 222 7e-58
sp|Q09510|MLRH_CAEEL Probable myosin regulatory light chain 222 7e-58
sp|Q9CQ19|MLRN_MOUSE Myosin regulatory light chain 2, smoot... 221 9e-58
sp|P29269|MLRN_PIG Myosin regulatory light chain 2, smooth ... 221 9e-58
sp|P24844|MLRN_HUMAN Myosin regulatory light chain 2, smoot... 221 9e-58
sp|P02612|MLRM_CHICK Myosin regulatory light chain 2, smoot... 221 2e-57
sp|P13832|MLRA_RAT Myosin regulatory light chain 2-A, smoot... 217 2e-56
sp|Q64122|MLRN_RAT Myosin regulatory light chain 2, smooth ... 212 5e-55
>sp|P24032|MLRN_CHICK Myosin regulatory light chain 2, smooth muscle minor isoform (G1)
(DTNB) (MLC-2) (Isoform L20-B1)
Length = 172
Score = 223 bits (568), Expect = 3e-58
Identities = 107/169 (63%), Positives = 136/169 (80%), Gaps = 1/169 (0%)
Frame = +2
Query: 41 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLG 220
A TKT KK R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLG
Sbjct: 6 AKTKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLG 62
Query: 221 KDANEAYIEEMLKQAAGTINFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVSEEH 400
K+ + Y++ M+ +A G INFTMFLT+FGEK+ G+DPED I NAFACFD + TGF+ E++
Sbjct: 63 KNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGFIQEDY 122
Query: 401 LRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 544
LR+L+T+MGDR+TDE+VDEL+ API G FNY EFT+++KHG K++D
Sbjct: 123 LRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171
>sp|P19105|MLRM_HUMAN Myosin regulatory light chain 2, nonsarcomeric (Myosin RLC)
sp|Q5RC34|MLRM_PONPY Myosin regulatory light chain 2, nonsarcomeric (Myosin RLC)
Length = 171
Score = 223 bits (567), Expect = 4e-58
Identities = 104/170 (61%), Positives = 137/170 (80%), Gaps = 1/170 (0%)
Frame = +2
Query: 38 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSL 217
M+S +T+ K + R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SL
Sbjct: 1 MSSKRTKTKTKKRPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASL 60
Query: 218 GKDANEAYIEEMLKQAAGTINFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVSEE 397
GK+ + Y++ M+ +A G INFTMFLT+FGEK+ G+DPED I NAFACFD + TG + E+
Sbjct: 61 GKNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQED 120
Query: 398 HLRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 544
+LR+L+T+MGDR+TDE+VDEL+ API G FNY EFT+++KHG K++D
Sbjct: 121 YLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 170
>sp|P18666|MLRB_RAT Myosin regulatory light chain 2-B, smooth muscle isoform (Myosin
RLC-B)
Length = 172
Score = 222 bits (565), Expect = 7e-58
Identities = 107/169 (63%), Positives = 136/169 (80%), Gaps = 1/169 (0%)
Frame = +2
Query: 41 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLG 220
A TKT KK R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLG
Sbjct: 6 AKTKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLG 62
Query: 221 KDANEAYIEEMLKQAAGTINFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVSEEH 400
K+ +AY++ M+ +A G INFTMFLT+FGEK+ G+DPED I NAFACFD + TG + E++
Sbjct: 63 KNPTDAYLDAMMNEAPGRINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDY 122
Query: 401 LRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 544
LR+L+T+MGDR+TDE+VDEL+ API G FNY EFT+++KHG K++D
Sbjct: 123 LRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171
>sp|Q09510|MLRH_CAEEL Probable myosin regulatory light chain
Length = 172
Score = 222 bits (565), Expect = 7e-58
Identities = 107/171 (62%), Positives = 138/171 (80%), Gaps = 2/171 (1%)
Frame = +2
Query: 38 MASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSL 217
MAS KT + R R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +M SL
Sbjct: 1 MASRKTVNR-RQRPQRATSNVFAMFDQAQIQEFKEAFNMIDQNRDGFIDQEDLKDMFASL 59
Query: 218 GKDANEAYIEEMLKQAAGT--INFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVS 391
GK+ E +I+ M+ +A G INFTMFLTLFGEK+TG+DPE+ I NAF CFD D++G ++
Sbjct: 60 GKEVTEQFIDSMINEAPGAQPINFTMFLTLFGEKLTGTDPEEVIRNAFQCFDEDNSGKLN 119
Query: 392 EEHLRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 544
EEHLR+L+T+MG+R+++EQVDELF API G+F+Y EFT+M+KHG K++D
Sbjct: 120 EEHLRELLTTMGERYSEEQVDELFRDAPIKGGQFDYVEFTRMLKHGTKDKD 170
>sp|Q9CQ19|MLRN_MOUSE Myosin regulatory light chain 2, smooth muscle isoform (Myosin RLC)
Length = 172
Score = 221 bits (564), Expect = 9e-58
Identities = 106/169 (62%), Positives = 135/169 (79%), Gaps = 1/169 (0%)
Frame = +2
Query: 41 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLG 220
A KT KK R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLG
Sbjct: 6 AKAKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLG 62
Query: 221 KDANEAYIEEMLKQAAGTINFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVSEEH 400
K+ + Y+E M+ +A G INFTMFLT+FGEK+ G+DPED I NAFACFD + +GF+ E+H
Sbjct: 63 KNPTDEYLEGMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDH 122
Query: 401 LRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 544
LR+L+T+MGDR+TDE+VDE++ API G FNY EFT+++KHG K++D
Sbjct: 123 LRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKD 171
>sp|P29269|MLRN_PIG Myosin regulatory light chain 2, smooth muscle isoform (Myosin RLC)
(LC20)
Length = 171
Score = 221 bits (564), Expect = 9e-58
Identities = 106/169 (62%), Positives = 135/169 (79%), Gaps = 1/169 (0%)
Frame = +2
Query: 41 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLG 220
A KT KK R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLG
Sbjct: 5 AKAKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLG 61
Query: 221 KDANEAYIEEMLKQAAGTINFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVSEEH 400
K+ + Y+E M+ +A G INFTMFLT+FGEK+ G+DPED I NAFACFD + +GF+ E+H
Sbjct: 62 KNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDH 121
Query: 401 LRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 544
LR+L+T+MGDR+TDE+VDE++ API G FNY EFT+++KHG K++D
Sbjct: 122 LRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKD 170
>sp|P24844|MLRN_HUMAN Myosin regulatory light chain 2, smooth muscle isoform (Myosin RLC)
(LC20)
Length = 172
Score = 221 bits (564), Expect = 9e-58
Identities = 106/169 (62%), Positives = 135/169 (79%), Gaps = 1/169 (0%)
Frame = +2
Query: 41 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLG 220
A KT KK R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLG
Sbjct: 6 AKAKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLG 62
Query: 221 KDANEAYIEEMLKQAAGTINFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVSEEH 400
K+ + Y+E M+ +A G INFTMFLT+FGEK+ G+DPED I NAFACFD + +GF+ E+H
Sbjct: 63 KNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDH 122
Query: 401 LRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 544
LR+L+T+MGDR+TDE+VDE++ API G FNY EFT+++KHG K++D
Sbjct: 123 LRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKD 171
>sp|P02612|MLRM_CHICK Myosin regulatory light chain 2, smooth muscle major isoform (G1)
(DTNB) (MLC-2) (Isoform L20-A)
Length = 172
Score = 221 bits (562), Expect = 2e-57
Identities = 105/169 (62%), Positives = 135/169 (79%), Gaps = 1/169 (0%)
Frame = +2
Query: 41 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLG 220
A KT KK R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML S+G
Sbjct: 6 AKAKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASMG 62
Query: 221 KDANEAYIEEMLKQAAGTINFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVSEEH 400
K+ + Y+E M+ +A G INFTMFLT+FGEK+ G+DPED I NAFACFD + +GF+ E+H
Sbjct: 63 KNPTDEYLEGMMSEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDH 122
Query: 401 LRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 544
LR+L+T+MGDR+TDE+VDE++ API G FNY EFT+++KHG K++D
Sbjct: 123 LRELLTTMGDRFTDEEVDEMYREAPIDKKGNFNYVEFTRILKHGAKDKD 171
>sp|P13832|MLRA_RAT Myosin regulatory light chain 2-A, smooth muscle isoform (Myosin
RLC-A)
Length = 172
Score = 217 bits (552), Expect = 2e-56
Identities = 104/169 (61%), Positives = 134/169 (79%), Gaps = 1/169 (0%)
Frame = +2
Query: 41 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLG 220
A TKT KK R QR TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML S+G
Sbjct: 6 AKTKTTKK---RPQRATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASMG 62
Query: 221 KDANEAYIEEMLKQAAGTINFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVSEEH 400
K+ + Y++ M+ +A G INFTMFLT+FGEK+ G+DPED I NAFACFD + G + E++
Sbjct: 63 KNPTDEYLDAMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEAIGTIQEDY 122
Query: 401 LRDLMTSMGDRWTDEQVDELFHGAPI-SNGKFNYREFTKMIKHGKKEED 544
LR+L+T+MGDR+TDE+VDEL+ API G FNY EFT+++KHG K++D
Sbjct: 123 LRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKD 171
>sp|Q64122|MLRN_RAT Myosin regulatory light chain 2, smooth muscle isoform (Myosin RLC)
Length = 171
Score = 212 bits (540), Expect = 5e-55
Identities = 101/168 (60%), Positives = 130/168 (77%)
Frame = +2
Query: 41 ASTKTRKKHRPRTQRYTSNVFSMFSEPQIQEFKEAFNMIDQNKDGFIDHDDLVEMLTSLG 220
A KT KK R Q TSNVF+MF + QIQEFKEAFNMIDQN+DGFID +DL +ML SLG
Sbjct: 6 AKAKTTKK---RPQSATSNVFAMFDQSQIQEFKEAFNMIDQNRDGFIDKEDLHDMLASLG 62
Query: 221 KDANEAYIEEMLKQAAGTINFTMFLTLFGEKMTGSDPEDTILNAFACFDPDDTGFVSEEH 400
K+ + +E M+ +A G INFTMFLT+FGEK+ G+DPED I NAFACFD + +GF+ E+H
Sbjct: 63 KNPTDEXLEGMMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEASGFIHEDH 122
Query: 401 LRDLMTSMGDRWTDEQVDELFHGAPISNGKFNYREFTKMIKHGKKEED 544
LR+L+T+MGDR+TDE+VDE++ G FNY EFT+++KHG K++D
Sbjct: 123 LRELLTTMGDRFTDEEVDEMYRERIDKKGNFNYVEFTRILKHGAKDKD 170
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 63,050,268
Number of Sequences: 369166
Number of extensions: 1203716
Number of successful extensions: 4322
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3753
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3995
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 4926080070
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)