Planaria EST Database


DrC_00535

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00535
         (1007 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q09543|2AAA_CAEEL  Probable protein phosphatase PP2A regu...    59   2e-08
sp|P54612|2AAA_PIG  Serine/threonine protein phosphatase 2A,...    47   7e-05
sp|P30153|2AAA_HUMAN  Serine/threonine protein phosphatase 2...    47   7e-05
sp|P36179|2AAA_DROME  Protein phosphatase PP2A, 65 kDa regul...    47   9e-05
sp|Q9UT08|2AAA_SCHPO  Protein phosphatase PP2A regulatory su...    46   2e-04
sp|P36875|2AAA_PEA  Protein phosphatase PP2A regulatory subu...    42   0.003
sp|P30154|2AAB_HUMAN  Serine/threonine protein phosphatase 2...    41   0.006
sp|Q9P1A2|PP4RL_HUMAN  Serine/threonine phosphatase 4 regula...    40   0.008
sp|P54613|2AAB_PIG  Serine/threonine protein phosphatase 2A,...    39   0.018
sp|Q9VKB9|RBGPR_DROME  Rab3 GTPase-activating protein regula...    31   6.4  
>sp|Q09543|2AAA_CAEEL Probable protein phosphatase PP2A regulatory subunit (Protein
           phosphatase PP2A regulatory subunit A)
          Length = 590

 Score = 58.9 bits (141), Expect = 2e-08
 Identities = 46/180 (25%), Positives = 84/180 (46%)
 Frame = +1

Query: 7   DKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGRF 186
           D  WR+R  +V  + L+   + +    + LL   L+WL D V++IR+ +  I+  +T +F
Sbjct: 416 DGKWRVRLAIVQFMPLLASQLGQEFFDEKLLPLCLNWLTDHVFSIREASTLIMKELTQKF 475

Query: 187 PGLLAEKLVNEKIFKELFEDVNYHFRQCWCFLIEAICKSQPKDCKSDLLTSFLTNHINNL 366
            G  A   +  K+ ++L +D NY  R    F +  + ++  ++         +   I  +
Sbjct: 476 GGQWASTNIVPKM-QKLQKDTNYLQRMTCLFCLNTLSEAMTQE--------QILKEIMPI 526

Query: 367 LLQMIEKDPVPNVQISAIRSIKLLSVYLDKTFXXXXXXXXXXXXSTSSSDIDVRDFSDTA 546
           +  ++E D VPNV+ +A +S+K +   L  +                 SD DVR FS+ A
Sbjct: 527 VKDLVE-DDVPNVRFNAAKSLKRIGKNLTPSTLTSEVKPLLEKLG-KDSDFDVRYFSEEA 584
>sp|P54612|2AAA_PIG Serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit
           A, alpha isoform (PP2A, subunit A, PR65-alpha isoform)
           (PP2A, subunit A, R1-alpha isoform)
 sp|Q76MZ3|2AAA_MOUSE Serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit
           A, alpha isoform (PP2A, subunit A, PR65-alpha isoform)
           (PP2A, subunit A, R1-alpha isoform)
          Length = 589

 Score = 47.4 bits (111), Expect = 7e-05
 Identities = 42/181 (23%), Positives = 75/181 (41%)
 Frame = +1

Query: 7   DKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGRF 186
           D  WR+R  ++  + L+   +      + L    ++WL D V+ IR+     +  +  +F
Sbjct: 414 DAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKF 473

Query: 187 PGLLAEKLVNEKIFKELFEDVNYHFRQCWCFLIEAICKSQPKDCKSDLLTSFLTNHINNL 366
               A   +  K+   +  D NY  R    F I  + +     C  D+     T H+   
Sbjct: 474 GKEWAHATIIPKVL-AMSGDPNYLHRMTTLFCINVLSEV----CGQDI----TTKHMLPT 524

Query: 367 LLQMIEKDPVPNVQISAIRSIKLLSVYLDKTFXXXXXXXXXXXXSTSSSDIDVRDFSDTA 546
           +L+M   DPV NV+ +  +S++ +   LD +              T   D+DV+ F+  A
Sbjct: 525 VLRM-AGDPVANVRFNVAKSLQKIGPILDNS-TLQSEVKPILEKLTQDQDVDVKYFAQEA 582

Query: 547 L 549
           L
Sbjct: 583 L 583
>sp|P30153|2AAA_HUMAN Serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit
           A, alpha isoform (PP2A, subunit A, PR65-alpha isoform)
           (PP2A, subunit A, R1-alpha isoform) (Medium tumor
           antigen-associated 61 kDa protein)
          Length = 589

 Score = 47.4 bits (111), Expect = 7e-05
 Identities = 42/181 (23%), Positives = 75/181 (41%)
 Frame = +1

Query: 7   DKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGRF 186
           D  WR+R  ++  + L+   +      + L    ++WL D V+ IR+     +  +  +F
Sbjct: 414 DAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKF 473

Query: 187 PGLLAEKLVNEKIFKELFEDVNYHFRQCWCFLIEAICKSQPKDCKSDLLTSFLTNHINNL 366
               A   +  K+   +  D NY  R    F I  + +     C  D+     T H+   
Sbjct: 474 GKEWAHATIIPKVL-AMSGDPNYLHRMTTLFCINVLSEV----CGQDI----TTKHMLPT 524

Query: 367 LLQMIEKDPVPNVQISAIRSIKLLSVYLDKTFXXXXXXXXXXXXSTSSSDIDVRDFSDTA 546
           +L+M   DPV NV+ +  +S++ +   LD +              T   D+DV+ F+  A
Sbjct: 525 VLRM-AGDPVANVRFNVAKSLQKIGPILDNS-TLQSEVKPILEKLTQDQDVDVKYFAQEA 582

Query: 547 L 549
           L
Sbjct: 583 L 583
>sp|P36179|2AAA_DROME Protein phosphatase PP2A, 65 kDa regulatory subunit (Protein
           phosphatase PP2A regulatory subunit A) (PR65)
          Length = 591

 Score = 47.0 bits (110), Expect = 9e-05
 Identities = 37/184 (20%), Positives = 78/184 (42%)
 Frame = +1

Query: 7   DKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGRF 186
           D  WR+R  ++  +  +   + +      L    + WL D V+ IR+     +  +  +F
Sbjct: 416 DSKWRVRLAIIEYMPALAGQLGQEFFDQKLRGLCMGWLNDHVYAIREAATLNMKKLVEQF 475

Query: 187 PGLLAEKLVNEKIFKELFEDVNYHFRQCWCFLIEAICKSQPKDCKSDLLTSFLTNHINNL 366
               AE+ +   I   +  + NY  R    F +  + +     C +D+ T  L   +   
Sbjct: 476 GAPWAEQAIIPMIL-VMSRNKNYLHRMTCLFCLNVLAEV----CGTDITTKLLLPTV--- 527

Query: 367 LLQMIEKDPVPNVQISAIRSIKLLSVYLDKTFXXXXXXXXXXXXSTSSSDIDVRDFSDTA 546
              ++  DPV NV+ +  ++++ +S +L+ +             +T  +D+DV+ F+  A
Sbjct: 528 --LLLAADPVANVRFNVAKTLQKISPFLEASVIDAQVKPTLDKLNT-DTDVDVKHFAAQA 584

Query: 547 LKDI 558
           +  I
Sbjct: 585 IAGI 588
>sp|Q9UT08|2AAA_SCHPO Protein phosphatase PP2A regulatory subunit A (PR65) (Protein
           phosphatase 2A 65 kDa regulatory subunit)
          Length = 590

 Score = 45.8 bits (107), Expect = 2e-04
 Identities = 43/186 (23%), Positives = 78/186 (41%), Gaps = 4/186 (2%)
 Frame = +1

Query: 7   DKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGRF 186
           DK WR+R  ++  + L+   +      + +    +SWL D V++IR+   + +  +T  F
Sbjct: 412 DKQWRVRLAIIDYIPLLAQQLGVEFFNEKMGNLCMSWLEDHVYSIREAAIKNLRKLTEIF 471

Query: 187 PGLLAEKLVNEKIFKELFEDVNYHFRQCWCFLIEAICKSQPKDCKSDLLTSFLTNHINNL 366
               A + +  K F  +    NY +R    F I  I  +        L    +   I   
Sbjct: 472 GLEWATETIIPK-FLAMRSHPNYLYRMTTIFAISEIAPA--------LNAEVIEKQILPT 522

Query: 367 LLQMIEKDPVPNVQISAIRSIKLLSVYL----DKTFXXXXXXXXXXXXSTSSSDIDVRDF 534
           L Q++  DP+PN++ +  ++ ++L   L    D T              T  +D DV+ F
Sbjct: 523 LEQLV-NDPIPNIRFNVAKAFEVLKPVLAAGGDSTVYEQQIIPLLEQL-TKDNDPDVQYF 580

Query: 535 SDTALK 552
           +  AL+
Sbjct: 581 ATQALE 586
>sp|P36875|2AAA_PEA Protein phosphatase PP2A regulatory subunit A (PR65)
          Length = 395

 Score = 42.0 bits (97), Expect = 0.003
 Identities = 40/186 (21%), Positives = 82/186 (44%), Gaps = 4/186 (2%)
 Frame = +1

Query: 7   DKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGR- 183
           D++WR+R  ++  + L+   +      D L    + WL+D  ++IR      +  +    
Sbjct: 215 DRHWRVRLAIIEYIPLLASQLGVGFFDDKLGALIMQWLKDKEYSIRNAAANNVKRLAAEE 274

Query: 184 -FPGLLAEKLVNEKIFKELFEDVNYHFRQCWCFLIEAICKSQPKDCKSDLLTSFLTNHIN 360
             P    + ++ + +  ++  D +Y +R     ++ AI    P      +L S +T+   
Sbjct: 275 FGPEWAMQHIIPQVL--DMINDPHYLYRMT---ILHAISLLAP------VLGSEITS--T 321

Query: 361 NLLLQMI--EKDPVPNVQISAIRSIKLLSVYLDKTFXXXXXXXXXXXXSTSSSDIDVRDF 534
           NLL  ++   KD VPN++ +  + ++ L   +D++             S    D+DVR F
Sbjct: 322 NLLPLVVNASKDRVPNIKFNVAKVLQSLIPIVDESVVESTIRPCLVELS-EDPDVDVRFF 380

Query: 535 SDTALK 552
           +  AL+
Sbjct: 381 ASQALQ 386

 Score = 34.3 bits (77), Expect = 0.58
 Identities = 37/145 (25%), Positives = 61/145 (42%), Gaps = 4/145 (2%)
 Frame = +1

Query: 7   DKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGRF 186
           DK+WR+R  V   L  +   V     +  L+   +  LRD V  +R       G ++ +F
Sbjct: 59  DKSWRVRYMVANQLYELCEAVGPDSTKTELVPAYVRLLRDNVAEVRIAAA---GKVS-KF 114

Query: 187 PGLLAEKLVNEKIF---KELFEDVNYHFRQCWCFLIEAICKSQPKDCK-SDLLTSFLTNH 354
             +L+ +L  + I    KEL  D + H R     +I  +     KD     LL  FL+  
Sbjct: 115 SRILSPELAIQHILPCVKELSTDSSQHVRSALASVIMGMAPVLGKDATIEQLLPIFLS-- 172

Query: 355 INNLLLQMIEKDPVPNVQISAIRSI 429
                   + KD  P+V+++ I  +
Sbjct: 173 --------LLKDEFPDVRLNIISKL 189
>sp|P30154|2AAB_HUMAN Serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit
           A, beta isoform (PP2A, subunit A, PR65-beta isoform)
           (PP2A, subunit A, R1-beta isoform)
          Length = 601

 Score = 40.8 bits (94), Expect = 0.006
 Identities = 39/181 (21%), Positives = 76/181 (41%)
 Frame = +1

Query: 7   DKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGRF 186
           D  WR+R  ++  + L+   +      + L    ++WL D V+ IR+     +  +  +F
Sbjct: 426 DAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATNNLMKLVQKF 485

Query: 187 PGLLAEKLVNEKIFKELFEDVNYHFRQCWCFLIEAICKSQPKDCKSDLLTSFLTNHINNL 366
               A+  +  K+   +  D NY  R    F I A+ ++    C  ++     T  +  +
Sbjct: 486 GTEWAQNTIVPKVL-VMANDPNYLHRMTTLFCINALSEA----CGQEI----TTKQMLPI 536

Query: 367 LLQMIEKDPVPNVQISAIRSIKLLSVYLDKTFXXXXXXXXXXXXSTSSSDIDVRDFSDTA 546
           +L+M   D V NV+ +  +S++ +   LD T                  D+DV+ F+  A
Sbjct: 537 VLKM-AGDQVANVRFNVAKSLQKIGPILD-TNALQGEVKPVLQKLGQDEDMDVKYFAQEA 594

Query: 547 L 549
           +
Sbjct: 595 I 595
>sp|Q9P1A2|PP4RL_HUMAN Serine/threonine phosphatase 4 regulatory subunit 1-like
          Length = 438

 Score = 40.4 bits (93), Expect = 0.008
 Identities = 41/157 (26%), Positives = 72/157 (45%), Gaps = 9/157 (5%)
 Frame = +1

Query: 4   SDKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGR 183
           + +NWR R  +   L+LI+ L       D L+   L    D V  +R  + +++ +I  +
Sbjct: 242 NSRNWRFRYELAEQLILILELYSPNDVYDYLMHIALKLCADQVSEVRWISFKLVVAILQK 301

Query: 184 F----PGLLAEKLVNEKI--FKELFEDVNYHFRQCWCFLIEAICKSQPKDCKSDLLTSFL 345
           F       L    +NE I  F+   + V    RQ + F+ +A+     K+C    +  F+
Sbjct: 302 FYSNSESALGLNFINELIIRFRHCSKWVG---RQAFAFICQAVVS---KECVP--VDQFM 353

Query: 346 TNHINNLLLQMIEKDPVPNVQI---SAIRSIKLLSVY 447
            + + +LL   +  DPVPNV++    A+R + L   Y
Sbjct: 354 EHLLPSLL--SLASDPVPNVRVLLAKALRQMLLEKAY 388
>sp|P54613|2AAB_PIG Serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit
           A, beta isoform (PP2A, subunit A, PR65-beta isoform)
           (PP2A, subunit A, R1-beta isoform)
          Length = 602

 Score = 39.3 bits (90), Expect = 0.018
 Identities = 38/181 (20%), Positives = 75/181 (41%)
 Frame = +1

Query: 7   DKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGRF 186
           D  WR+R  ++  + L+   +      + L    ++WL D V+ IR+     +  +  +F
Sbjct: 427 DAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATNNLMKLVQKF 486

Query: 187 PGLLAEKLVNEKIFKELFEDVNYHFRQCWCFLIEAICKSQPKDCKSDLLTSFLTNHINNL 366
               A+  +  K+   +  D NY  R    F I  + ++    C  ++     T  +  +
Sbjct: 487 GTEWAQNTIVPKVL-VMANDPNYLHRMTTLFCINVLSEA----CGQEI----TTKQMLPI 537

Query: 367 LLQMIEKDPVPNVQISAIRSIKLLSVYLDKTFXXXXXXXXXXXXSTSSSDIDVRDFSDTA 546
           +L+M   D V NV+ +  +S++ +   LD T                  D+DV+ F+  A
Sbjct: 538 VLKM-AGDQVANVRFNVAKSLQKIGPILD-TDALQEEVKPVLQKLGQDEDMDVKYFAQEA 595

Query: 547 L 549
           +
Sbjct: 596 I 596
>sp|Q9VKB9|RBGPR_DROME Rab3 GTPase-activating protein regulatory subunit
          Length = 1341

 Score = 30.8 bits (68), Expect = 6.4
 Identities = 22/71 (30%), Positives = 35/71 (49%), Gaps = 5/71 (7%)
 Frame = +1

Query: 22   IRSGVVASLMLIVPLVDEACCRDSL-----LLTTLSWLRDPVWNIRQGTCEIIGSITGRF 186
            + SGV+ SLM    +V  +    SL      L  L+  R P W ++ G C ++ + T +F
Sbjct: 962  LESGVLLSLMSWQYMVQWSKQLSSLDHLKAALLCLNQFRTPDWALKHGICCMLWNATLKF 1021

Query: 187  PGLLAEKLVNE 219
            P   A KL+ +
Sbjct: 1022 PLQAAAKLIQK 1032
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 110,789,037
Number of Sequences: 369166
Number of extensions: 2157769
Number of successful extensions: 4718
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4518
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4715
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 10718264480
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00535

  1. Dr_sW_013_N13
  2. Dr_sW_006_P22