Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_013_N13
(776 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q09543|2AAA_CAEEL Probable protein phosphatase PP2A regu... 59 1e-08
sp|P54612|2AAA_PIG Serine/threonine protein phosphatase 2A,... 47 4e-05
sp|P30153|2AAA_HUMAN Serine/threonine protein phosphatase 2... 47 4e-05
sp|P36179|2AAA_DROME Protein phosphatase PP2A, 65 kDa regul... 47 6e-05
sp|Q9UT08|2AAA_SCHPO Protein phosphatase PP2A regulatory su... 46 1e-04
sp|P36875|2AAA_PEA Protein phosphatase PP2A regulatory subu... 42 0.002
sp|P30154|2AAB_HUMAN Serine/threonine protein phosphatase 2... 41 0.004
sp|Q9P1A2|PP4RL_HUMAN Serine/threonine phosphatase 4 regula... 40 0.005
sp|P54613|2AAB_PIG Serine/threonine protein phosphatase 2A,... 39 0.012
sp|Q9VKB9|RBGPR_DROME Rab3 GTPase-activating protein regula... 31 4.3
>sp|Q09543|2AAA_CAEEL Probable protein phosphatase PP2A regulatory subunit (Protein
phosphatase PP2A regulatory subunit A)
Length = 590
Score = 58.9 bits (141), Expect = 1e-08
Identities = 46/180 (25%), Positives = 84/180 (46%)
Frame = +1
Query: 7 DKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGRF 186
D WR+R +V + L+ + + + LL L+WL D V++IR+ + I+ +T +F
Sbjct: 416 DGKWRVRLAIVQFMPLLASQLGQEFFDEKLLPLCLNWLTDHVFSIREASTLIMKELTQKF 475
Query: 187 PGLLAEKLVNEKIFKELFEDVNYHFRQCWCFLIEAICKSQPKDCKSDLLTSFLTNHINNL 366
G A + K+ ++L +D NY R F + + ++ ++ + I +
Sbjct: 476 GGQWASTNIVPKM-QKLQKDTNYLQRMTCLFCLNTLSEAMTQE--------QILKEIMPI 526
Query: 367 LLQMIEKDPVPNVQISAIRSIKLLSVYLDKTFXXXXXXXXXXXXSTSSSDIDVRDFSDTA 546
+ ++E D VPNV+ +A +S+K + L + SD DVR FS+ A
Sbjct: 527 VKDLVE-DDVPNVRFNAAKSLKRIGKNLTPSTLTSEVKPLLEKLG-KDSDFDVRYFSEEA 584
Score = 29.6 bits (65), Expect = 9.6
Identities = 23/89 (25%), Positives = 38/89 (42%), Gaps = 5/89 (5%)
Frame = +1
Query: 1 FSDKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITG 180
F DK+WR+R V L+ I ++ E L+ + L+DP +R + +
Sbjct: 254 FDDKSWRVRYMVAEKLIEIQNVLGEDMDTTHLVNMYTNLLKDPEGEVRCAATQRLQEFAL 313
Query: 181 RFP-----GLLAEKLVNEKIFKELFEDVN 252
P ++ L+N + KEL D N
Sbjct: 314 NLPEDKRQNIICNSLLN--VAKELVTDGN 340
>sp|P54612|2AAA_PIG Serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit
A, alpha isoform (PP2A, subunit A, PR65-alpha isoform)
(PP2A, subunit A, R1-alpha isoform)
sp|Q76MZ3|2AAA_MOUSE Serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit
A, alpha isoform (PP2A, subunit A, PR65-alpha isoform)
(PP2A, subunit A, R1-alpha isoform)
Length = 589
Score = 47.4 bits (111), Expect = 4e-05
Identities = 42/181 (23%), Positives = 75/181 (41%)
Frame = +1
Query: 7 DKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGRF 186
D WR+R ++ + L+ + + L ++WL D V+ IR+ + + +F
Sbjct: 414 DAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKF 473
Query: 187 PGLLAEKLVNEKIFKELFEDVNYHFRQCWCFLIEAICKSQPKDCKSDLLTSFLTNHINNL 366
A + K+ + D NY R F I + + C D+ T H+
Sbjct: 474 GKEWAHATIIPKVL-AMSGDPNYLHRMTTLFCINVLSEV----CGQDI----TTKHMLPT 524
Query: 367 LLQMIEKDPVPNVQISAIRSIKLLSVYLDKTFXXXXXXXXXXXXSTSSSDIDVRDFSDTA 546
+L+M DPV NV+ + +S++ + LD + T D+DV+ F+ A
Sbjct: 525 VLRM-AGDPVANVRFNVAKSLQKIGPILDNS-TLQSEVKPILEKLTQDQDVDVKYFAQEA 582
Query: 547 L 549
L
Sbjct: 583 L 583
>sp|P30153|2AAA_HUMAN Serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit
A, alpha isoform (PP2A, subunit A, PR65-alpha isoform)
(PP2A, subunit A, R1-alpha isoform) (Medium tumor
antigen-associated 61 kDa protein)
Length = 589
Score = 47.4 bits (111), Expect = 4e-05
Identities = 42/181 (23%), Positives = 75/181 (41%)
Frame = +1
Query: 7 DKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGRF 186
D WR+R ++ + L+ + + L ++WL D V+ IR+ + + +F
Sbjct: 414 DAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATSNLKKLVEKF 473
Query: 187 PGLLAEKLVNEKIFKELFEDVNYHFRQCWCFLIEAICKSQPKDCKSDLLTSFLTNHINNL 366
A + K+ + D NY R F I + + C D+ T H+
Sbjct: 474 GKEWAHATIIPKVL-AMSGDPNYLHRMTTLFCINVLSEV----CGQDI----TTKHMLPT 524
Query: 367 LLQMIEKDPVPNVQISAIRSIKLLSVYLDKTFXXXXXXXXXXXXSTSSSDIDVRDFSDTA 546
+L+M DPV NV+ + +S++ + LD + T D+DV+ F+ A
Sbjct: 525 VLRM-AGDPVANVRFNVAKSLQKIGPILDNS-TLQSEVKPILEKLTQDQDVDVKYFAQEA 582
Query: 547 L 549
L
Sbjct: 583 L 583
>sp|P36179|2AAA_DROME Protein phosphatase PP2A, 65 kDa regulatory subunit (Protein
phosphatase PP2A regulatory subunit A) (PR65)
Length = 591
Score = 47.0 bits (110), Expect = 6e-05
Identities = 37/184 (20%), Positives = 78/184 (42%)
Frame = +1
Query: 7 DKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGRF 186
D WR+R ++ + + + + L + WL D V+ IR+ + + +F
Sbjct: 416 DSKWRVRLAIIEYMPALAGQLGQEFFDQKLRGLCMGWLNDHVYAIREAATLNMKKLVEQF 475
Query: 187 PGLLAEKLVNEKIFKELFEDVNYHFRQCWCFLIEAICKSQPKDCKSDLLTSFLTNHINNL 366
AE+ + I + + NY R F + + + C +D+ T L +
Sbjct: 476 GAPWAEQAIIPMIL-VMSRNKNYLHRMTCLFCLNVLAEV----CGTDITTKLLLPTV--- 527
Query: 367 LLQMIEKDPVPNVQISAIRSIKLLSVYLDKTFXXXXXXXXXXXXSTSSSDIDVRDFSDTA 546
++ DPV NV+ + ++++ +S +L+ + +T +D+DV+ F+ A
Sbjct: 528 --LLLAADPVANVRFNVAKTLQKISPFLEASVIDAQVKPTLDKLNT-DTDVDVKHFAAQA 584
Query: 547 LKDI 558
+ I
Sbjct: 585 IAGI 588
>sp|Q9UT08|2AAA_SCHPO Protein phosphatase PP2A regulatory subunit A (PR65) (Protein
phosphatase 2A 65 kDa regulatory subunit)
Length = 590
Score = 45.8 bits (107), Expect = 1e-04
Identities = 43/186 (23%), Positives = 78/186 (41%), Gaps = 4/186 (2%)
Frame = +1
Query: 7 DKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGRF 186
DK WR+R ++ + L+ + + + +SWL D V++IR+ + + +T F
Sbjct: 412 DKQWRVRLAIIDYIPLLAQQLGVEFFNEKMGNLCMSWLEDHVYSIREAAIKNLRKLTEIF 471
Query: 187 PGLLAEKLVNEKIFKELFEDVNYHFRQCWCFLIEAICKSQPKDCKSDLLTSFLTNHINNL 366
A + + K F + NY +R F I I + L + I
Sbjct: 472 GLEWATETIIPK-FLAMRSHPNYLYRMTTIFAISEIAPA--------LNAEVIEKQILPT 522
Query: 367 LLQMIEKDPVPNVQISAIRSIKLLSVYL----DKTFXXXXXXXXXXXXSTSSSDIDVRDF 534
L Q++ DP+PN++ + ++ ++L L D T T +D DV+ F
Sbjct: 523 LEQLV-NDPIPNIRFNVAKAFEVLKPVLAAGGDSTVYEQQIIPLLEQL-TKDNDPDVQYF 580
Query: 535 SDTALK 552
+ AL+
Sbjct: 581 ATQALE 586
>sp|P36875|2AAA_PEA Protein phosphatase PP2A regulatory subunit A (PR65)
Length = 395
Score = 42.0 bits (97), Expect = 0.002
Identities = 40/186 (21%), Positives = 82/186 (44%), Gaps = 4/186 (2%)
Frame = +1
Query: 7 DKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGR- 183
D++WR+R ++ + L+ + D L + WL+D ++IR + +
Sbjct: 215 DRHWRVRLAIIEYIPLLASQLGVGFFDDKLGALIMQWLKDKEYSIRNAAANNVKRLAAEE 274
Query: 184 -FPGLLAEKLVNEKIFKELFEDVNYHFRQCWCFLIEAICKSQPKDCKSDLLTSFLTNHIN 360
P + ++ + + ++ D +Y +R ++ AI P +L S +T+
Sbjct: 275 FGPEWAMQHIIPQVL--DMINDPHYLYRMT---ILHAISLLAP------VLGSEITS--T 321
Query: 361 NLLLQMI--EKDPVPNVQISAIRSIKLLSVYLDKTFXXXXXXXXXXXXSTSSSDIDVRDF 534
NLL ++ KD VPN++ + + ++ L +D++ S D+DVR F
Sbjct: 322 NLLPLVVNASKDRVPNIKFNVAKVLQSLIPIVDESVVESTIRPCLVELS-EDPDVDVRFF 380
Query: 535 SDTALK 552
+ AL+
Sbjct: 381 ASQALQ 386
Score = 34.3 bits (77), Expect = 0.39
Identities = 37/145 (25%), Positives = 61/145 (42%), Gaps = 4/145 (2%)
Frame = +1
Query: 7 DKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGRF 186
DK+WR+R V L + V + L+ + LRD V +R G ++ +F
Sbjct: 59 DKSWRVRYMVANQLYELCEAVGPDSTKTELVPAYVRLLRDNVAEVRIAAA---GKVS-KF 114
Query: 187 PGLLAEKLVNEKIF---KELFEDVNYHFRQCWCFLIEAICKSQPKDCK-SDLLTSFLTNH 354
+L+ +L + I KEL D + H R +I + KD LL FL+
Sbjct: 115 SRILSPELAIQHILPCVKELSTDSSQHVRSALASVIMGMAPVLGKDATIEQLLPIFLS-- 172
Query: 355 INNLLLQMIEKDPVPNVQISAIRSI 429
+ KD P+V+++ I +
Sbjct: 173 --------LLKDEFPDVRLNIISKL 189
>sp|P30154|2AAB_HUMAN Serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit
A, beta isoform (PP2A, subunit A, PR65-beta isoform)
(PP2A, subunit A, R1-beta isoform)
Length = 601
Score = 40.8 bits (94), Expect = 0.004
Identities = 39/181 (21%), Positives = 76/181 (41%)
Frame = +1
Query: 7 DKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGRF 186
D WR+R ++ + L+ + + L ++WL D V+ IR+ + + +F
Sbjct: 426 DAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATNNLMKLVQKF 485
Query: 187 PGLLAEKLVNEKIFKELFEDVNYHFRQCWCFLIEAICKSQPKDCKSDLLTSFLTNHINNL 366
A+ + K+ + D NY R F I A+ ++ C ++ T + +
Sbjct: 486 GTEWAQNTIVPKVL-VMANDPNYLHRMTTLFCINALSEA----CGQEI----TTKQMLPI 536
Query: 367 LLQMIEKDPVPNVQISAIRSIKLLSVYLDKTFXXXXXXXXXXXXSTSSSDIDVRDFSDTA 546
+L+M D V NV+ + +S++ + LD T D+DV+ F+ A
Sbjct: 537 VLKM-AGDQVANVRFNVAKSLQKIGPILD-TNALQGEVKPVLQKLGQDEDMDVKYFAQEA 594
Query: 547 L 549
+
Sbjct: 595 I 595
>sp|Q9P1A2|PP4RL_HUMAN Serine/threonine phosphatase 4 regulatory subunit 1-like
Length = 438
Score = 40.4 bits (93), Expect = 0.005
Identities = 41/157 (26%), Positives = 72/157 (45%), Gaps = 9/157 (5%)
Frame = +1
Query: 4 SDKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGR 183
+ +NWR R + L+LI+ L D L+ L D V +R + +++ +I +
Sbjct: 242 NSRNWRFRYELAEQLILILELYSPNDVYDYLMHIALKLCADQVSEVRWISFKLVVAILQK 301
Query: 184 F----PGLLAEKLVNEKI--FKELFEDVNYHFRQCWCFLIEAICKSQPKDCKSDLLTSFL 345
F L +NE I F+ + V RQ + F+ +A+ K+C + F+
Sbjct: 302 FYSNSESALGLNFINELIIRFRHCSKWVG---RQAFAFICQAVVS---KECVP--VDQFM 353
Query: 346 TNHINNLLLQMIEKDPVPNVQI---SAIRSIKLLSVY 447
+ + +LL + DPVPNV++ A+R + L Y
Sbjct: 354 EHLLPSLL--SLASDPVPNVRVLLAKALRQMLLEKAY 388
>sp|P54613|2AAB_PIG Serine/threonine protein phosphatase 2A, 65 kDa regulatory subunit
A, beta isoform (PP2A, subunit A, PR65-beta isoform)
(PP2A, subunit A, R1-beta isoform)
Length = 602
Score = 39.3 bits (90), Expect = 0.012
Identities = 38/181 (20%), Positives = 75/181 (41%)
Frame = +1
Query: 7 DKNWRIRSGVVASLMLIVPLVDEACCRDSLLLTTLSWLRDPVWNIRQGTCEIIGSITGRF 186
D WR+R ++ + L+ + + L ++WL D V+ IR+ + + +F
Sbjct: 427 DAKWRVRLAIIEYMPLLAGQLGVEFFDEKLNSLCMAWLVDHVYAIREAATNNLMKLVQKF 486
Query: 187 PGLLAEKLVNEKIFKELFEDVNYHFRQCWCFLIEAICKSQPKDCKSDLLTSFLTNHINNL 366
A+ + K+ + D NY R F I + ++ C ++ T + +
Sbjct: 487 GTEWAQNTIVPKVL-VMANDPNYLHRMTTLFCINVLSEA----CGQEI----TTKQMLPI 537
Query: 367 LLQMIEKDPVPNVQISAIRSIKLLSVYLDKTFXXXXXXXXXXXXSTSSSDIDVRDFSDTA 546
+L+M D V NV+ + +S++ + LD T D+DV+ F+ A
Sbjct: 538 VLKM-AGDQVANVRFNVAKSLQKIGPILD-TDALQEEVKPVLQKLGQDEDMDVKYFAQEA 595
Query: 547 L 549
+
Sbjct: 596 I 596
>sp|Q9VKB9|RBGPR_DROME Rab3 GTPase-activating protein regulatory subunit
Length = 1341
Score = 30.8 bits (68), Expect = 4.3
Identities = 22/71 (30%), Positives = 35/71 (49%), Gaps = 5/71 (7%)
Frame = +1
Query: 22 IRSGVVASLMLIVPLVDEACCRDSL-----LLTTLSWLRDPVWNIRQGTCEIIGSITGRF 186
+ SGV+ SLM +V + SL L L+ R P W ++ G C ++ + T +F
Sbjct: 962 LESGVLLSLMSWQYMVQWSKQLSSLDHLKAALLCLNQFRTPDWALKHGICCMLWNATLKF 1021
Query: 187 PGLLAEKLVNE 219
P A KL+ +
Sbjct: 1022 PLQAAAKLIQK 1032
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 87,943,274
Number of Sequences: 369166
Number of extensions: 1743826
Number of successful extensions: 3839
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3706
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3837
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7260540000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)