Planaria EST Database

DrC_00358

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00358
         (1025 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O75586|MED6_HUMAN  RNA polymerase transcriptional regulat...   134   4e-31
sp|Q5RD94|MED6_PONPY  RNA polymerase transcriptional regulat...   134   4e-31
sp|Q9US45|PMC5_SCHPO  RNA polymerase II mediator complex pro...    79   2e-14
sp|P38782|MED6_YEAST  RNA polymerase II mediator complex pro...    70   7e-12
sp|P25558|BUD3_YEAST  Bud site selection protein BUD3              34   0.59 
sp|P34314|TLK1_CAEEL  Serine/threonine-protein kinase tousle...    34   0.59 
sp|Q99MA9|NKX61_MOUSE  Homeobox protein Nkx-6.1                    33   1.0  
sp|Q16665|HIF1A_HUMAN  Hypoxia-inducible factor 1 alpha (HIF...    33   1.3  
sp|O35762|NKX61_RAT  Homeobox protein Nkx-6.1                      32   2.2  
sp|P32770|NRP1_YEAST  Asparagine-rich protein (ARP protein)        32   2.2  

>sp|O75586|MED6_HUMAN RNA polymerase transcriptional regulation mediator, subunit 6
           homolog (Activator-recruited cofactor 33 kDa component)
           (ARC33) (NY-REN-28 antigen)
          Length = 246

 Score =  134 bits (337), Expect = 4e-31
 Identities = 69/181 (38%), Positives = 108/181 (59%), Gaps = 1/181 (0%)
 Frame = +1

Query: 292 LTTKWKNPMWDPNMLNSGNVLNYFCDRSNPFYNHQCNNEKAMMQKFSGD-ITSLQGEEYV 468
           L   W +  W P +LNSG+VL+YF +RSNPFY+  CNNE   MQ+ + + +  + G EY+
Sbjct: 11  LGISWVDSSWIP-ILNSGSVLDYFSERSNPFYDRTCNNEVVKMQRLTLEHLNQMVGIEYI 69

Query: 469 LFHAQDPILFIICKQRRKSNTQTTPLAYYYIVNGEVMQAPDVASLINARLQTSLYHMNET 648
           L HAQ+PILFII KQ+R+S  Q  PLA YYI+ G + QAPD+ S+IN+R+ T+++ +   
Sbjct: 70  LLHAQEPILFIIRKQQRQSPAQVIPLADYYIIAGVIYQAPDLGSVINSRVLTAVHGIQSA 129

Query: 649 FQSLLQQVQHHPSSGTYSWNDESGVKSNKFDSVEEHQRNFGNQFHTTNMTQLMSEWADNY 828
           F   +   ++HPS G Y W+ +   + +K     + +    + F    +  L+ +    +
Sbjct: 130 FDEAMSYCRYHPSKG-YWWHFKDHEEQDKVRPKAKRKEEPSSIFQRQRVDALLLDLRQKF 188

Query: 829 P 831
           P
Sbjct: 189 P 189

>sp|Q5RD94|MED6_PONPY RNA polymerase transcriptional regulation mediator, subunit 6
           homolog
          Length = 246

 Score =  134 bits (337), Expect = 4e-31
 Identities = 69/181 (38%), Positives = 108/181 (59%), Gaps = 1/181 (0%)
 Frame = +1

Query: 292 LTTKWKNPMWDPNMLNSGNVLNYFCDRSNPFYNHQCNNEKAMMQKFSGD-ITSLQGEEYV 468
           L   W +  W P +LNSG+VL+YF +RSNPFY+  CNNE   MQ+ + + +  + G EY+
Sbjct: 11  LGISWVDSSWIP-ILNSGSVLDYFSERSNPFYDRTCNNEVVKMQRLTLEHLNQMVGIEYI 69

Query: 469 LFHAQDPILFIICKQRRKSNTQTTPLAYYYIVNGEVMQAPDVASLINARLQTSLYHMNET 648
           L HAQ+PILFII KQ+R+S  Q  PLA YYI+ G + QAPD+ S+IN+R+ T+++ +   
Sbjct: 70  LLHAQEPILFIIRKQQRQSPAQVIPLADYYIIAGVIYQAPDLGSVINSRVLTAVHGIQSA 129

Query: 649 FQSLLQQVQHHPSSGTYSWNDESGVKSNKFDSVEEHQRNFGNQFHTTNMTQLMSEWADNY 828
           F   +   ++HPS G Y W+ +   + +K     + +    + F    +  L+ +    +
Sbjct: 130 FDEAMSYCRYHPSKG-YWWHFKEHEEQDKVRPKAKRKEEPSSIFQRQRVDALLLDLRQKF 188

Query: 829 P 831
           P
Sbjct: 189 P 189

>sp|Q9US45|PMC5_SCHPO RNA polymerase II mediator complex protein pmc5 (RNA polymerase II
           mediator complex protein med6) (RNA polymerase II
           transcriptional regulation mediator 6)
          Length = 216

 Score = 79.3 bits (194), Expect = 2e-14
 Identities = 45/142 (31%), Positives = 76/142 (53%), Gaps = 11/142 (7%)
 Frame = +1

Query: 301 KWKNPMWDPNM--LNSGNVLNYFCDRSNPFYNHQCNNEKAMMQ-KFS----GDITS---- 447
           +W+ P W  +M  L + NVL YF    +PFY+H+ NNE   MQ +F+    GD+ S    
Sbjct: 15  QWRMPEWVQSMGGLRTENVLEYFSQ--SPFYSHKSNNEMLKMQSQFNALDLGDLNSQLKR 72

Query: 448 LQGEEYVLFHAQDPILFIICKQRRKSNTQTTPLAYYYIVNGEVMQAPDVASLINARLQTS 627
           L G ++V+ H + P L++I KQ R +  +  PL  Y++ N  +  AP+  +L+  R+  +
Sbjct: 73  LTGIQFVIIHERPPFLWVIQKQNRLNENEVKPLTVYFVCNENIYMAPNAYTLLATRMLNA 132

Query: 628 LYHMNETFQSLLQQVQHHPSSG 693
            Y   +    + +  Q++P  G
Sbjct: 133 TYCFQKALTKIEKFPQYNPQEG 154

>sp|P38782|MED6_YEAST RNA polymerase II mediator complex protein MED6 (RNA polymerase II
           transcriptional regulation mediator 6)
          Length = 295

 Score = 70.5 bits (171), Expect = 7e-12
 Identities = 47/194 (24%), Positives = 80/194 (41%), Gaps = 61/194 (31%)
 Frame = +1

Query: 301 KWKNPMWDPNM-LNSGNVLNYFCDRSNPFYNHQCNNEKAMMQ------------------ 423
           +WK+P W     L + NVL+YF +  +PF++   NN+   MQ                  
Sbjct: 10  QWKSPEWIQVFGLRTENVLDYFAE--SPFFDKTSNNQVIKMQRQFSQLNDPNAAVNMTQN 67

Query: 424 ----------------------------------KFSGDITSLQGEEYVLFHAQDPILFI 501
                                             +   ++  L G EYVL   ++P  ++
Sbjct: 68  IMTLPDGKNGNLEEEFAYVDPARRQILFKYPMYMQLEEELMKLDGTEYVLSSVREPDFWV 127

Query: 502 ICKQRRKSNT--------QTTPLAYYYIVNGEVMQAPDVASLINARLQTSLYHMNETFQS 657
           I KQRR +N+        +  PL  YYI+   + Q+P +  ++ +RL ++ YH+N T +S
Sbjct: 128 IRKQRRTNNSGVGSAKGPEIIPLQDYYIIGANIYQSPTIFKIVQSRLMSTSYHLNSTLES 187

Query: 658 LLQQVQHHPSSGTY 699
           L   ++  PS G +
Sbjct: 188 LYDLIEFQPSQGVH 201

>sp|P25558|BUD3_YEAST Bud site selection protein BUD3
          Length = 1636

 Score = 34.3 bits (77), Expect = 0.59
 Identities = 33/148 (22%), Positives = 62/148 (41%), Gaps = 8/148 (5%)
 Frame = +1

Query: 490  ILFIICKQRRKSNTQTTPLAYYYIVNGEVMQAPDVASLINARLQTSLYHMNETFQSLLQQ 669
            IL ++ K   K    T+    + I+N   ++ P   S +N+ +   L  +NE   +L++ 
Sbjct: 683  ILDVLTKHDDKHIEVTSDNIVFTIINQLAIEIPICFSSLNSSMAKDLLCVNE---NLIKN 739

Query: 670  VQH------HPSSGTYSWNDESGVKSNKFDSVEEHQRNFG--NQFHTTNMTQLMSEWADN 825
            ++H      HPS+  +   +     ++ FD+  E +R++G    F +       S   DN
Sbjct: 740  LEHQLEEVKHPSTDEHRAVNSKLSGASDFDATHEKKRSYGTITTFRSYTSDLKDSPSGDN 799

Query: 826  YPCYLPQYNVLAMNPPAASSNTGRHISQ 909
                     +L + P   SS   R I +
Sbjct: 800  SNVTKETKEILPVKPTKKSSKKpreIQK 827

>sp|P34314|TLK1_CAEEL Serine/threonine-protein kinase tousled-like 1 (Tousled-like kinase
           1)
          Length = 965

 Score = 34.3 bits (77), Expect = 0.59
 Identities = 32/147 (21%), Positives = 50/147 (34%), Gaps = 7/147 (4%)
 Frame = +1

Query: 571 EVMQAPDVASLINARLQTSLYHMNETFQSLLQQVQHHPSSGTYSWNDESGVKSNKFDSVE 750
           + MQA    S+      + ++HM+     + QQ QHH      S+++             
Sbjct: 126 QCMQAMSEDSIEMRDYNSGVHHMHPHQMQMQQQQQHHQQQYNMSYHN------------- 172

Query: 751 EHQRNFGNQFHTTNMTQLMSEWADNYPCYLPQYNVLAMNPPAASSNTGRHISQTPSVG-- 924
            HQ+      +     Q   + A ++  Y PQ       P   S        Q  S    
Sbjct: 173 -HQQQMQQMHYHQQQQQYQQQQAQHHQMYAPQIQQQQQQPQQQSQQQSAQQPQQSSAALQ 231

Query: 925 SCTPRSNIATAAGPSDL-----GGQPQ 990
                SN+++A   SD      GG PQ
Sbjct: 232 HVNESSNLSSAGSISDREPEQHGGTPQ 258

>sp|Q99MA9|NKX61_MOUSE Homeobox protein Nkx-6.1
          Length = 365

 Score = 33.5 bits (75), Expect = 1.0
 Identities = 21/55 (38%), Positives = 27/55 (49%), Gaps = 1/55 (1%)
 Frame = +1

Query: 829 PCYLPQYNVLAMNPPAASSNTGRHISQTPSVGSCTPRSNIATAAGP-SDLGGQPQ 990
           P Y   Y  L   PP++SS++    S +P +GS  P      AAG  S LG  PQ
Sbjct: 34  PLYPAAYPPLPTGPPSSSSSSSSSSSPSPPLGSHNPGGLKPPAAGGLSSLGSPPQ 88

>sp|Q16665|HIF1A_HUMAN Hypoxia-inducible factor 1 alpha (HIF-1 alpha) (HIF1 alpha) (ARNT
           interacting protein) (Member of PAS protein 1) (MOP1)
          Length = 826

 Score = 33.1 bits (74), Expect = 1.3
 Identities = 18/61 (29%), Positives = 32/61 (52%), Gaps = 5/61 (8%)
 Frame = +1

Query: 586 PDVASLINARLQTSLYHMNETFQS-----LLQQVQHHPSSGTYSWNDESGVKSNKFDSVE 750
           P + +L NA+ +  + H    FQ+     LLQQ   H ++ + SW    G KS++ + +E
Sbjct: 708 PKILALQNAQRKRKMEHDGSLFQAVGIGTLLQQPDDHAATTSLSWKRVKGCKSSEQNGME 767

Query: 751 E 753
           +
Sbjct: 768 Q 768

>sp|O35762|NKX61_RAT Homeobox protein Nkx-6.1
          Length = 365

 Score = 32.3 bits (72), Expect = 2.2
 Identities = 20/55 (36%), Positives = 27/55 (49%), Gaps = 1/55 (1%)
 Frame = +1

Query: 829 PCYLPQYNVLAMNPPAASSNTGRHISQTPSVGSCTPRSNIATAAGP-SDLGGQPQ 990
           P Y   Y  L   PP++SS++    S +P +G+  P      AAG  S LG  PQ
Sbjct: 34  PLYPAAYPPLPTGPPSSSSSSSSSSSPSPPLGAHNPGGLKPPAAGGLSSLGSPPQ 88

>sp|P32770|NRP1_YEAST Asparagine-rich protein (ARP protein)
          Length = 719

 Score = 32.3 bits (72), Expect = 2.2
 Identities = 21/84 (25%), Positives = 45/84 (53%)
 Frame = +1

Query: 727 SNKFDSVEEHQRNFGNQFHTTNMTQLMSEWADNYPCYLPQYNVLAMNPPAASSNTGRHIS 906
           +N  ++V   + N  N+ ++ + + + S  A N+P   P++N++A N PAA +    H  
Sbjct: 391 ANSNNNVNSSRNNLNNRVNSGSSSNI-SNTAANHPYGAPEFNMIANNTPAALTYNRAHF- 448

Query: 907 QTPSVGSCTPRSNIATAAGPSDLG 978
             P++   + ++++  A  PS+ G
Sbjct: 449 --PAITPLSRQNSLNMA--PSNSG 468

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 113,654,573
Number of Sequences: 369166
Number of extensions: 2277765
Number of successful extensions: 5478
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5250
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5470
length of database: 68,354,980
effective HSP length: 111
effective length of database: 47,849,395
effective search space used: 11005360850
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00358

  1. Dr_sW_022_A05
  2. Dr_sW_009_H20
  3. Dr_sW_007_P24