Planaria EST Database


DrC_00326

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00326
         (708 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O95169|NIAM_HUMAN  NADH-ubiquinone oxidoreductase ASHI su...    99   1e-20
sp|Q02372|NIAM_BOVIN  NADH-ubiquinone oxidoreductase ASHI su...    95   2e-19
sp|Q9D6J5|NIAM_MOUSE  NADH-ubiquinone oxidoreductase ASHI su...    90   7e-18
sp|Q04464|VGLB_SHV1  Glycoprotein B precursor                      35   0.20 
sp|Q25390|SCRA_LIMPO  Alpha-scruin                                 33   0.74 
sp|P39352|YJHB_ECOLI  Hypothetical metabolite transport prot...    32   2.2  
sp|Q8CPC7|TKT_STAES  Transketolase (TK)                            31   2.8  
sp|Q5HPJ9|TKT_STAEQ  Transketolase                                 31   2.8  
sp|P26307|VIV1_MAIZE  Regulatory protein viviparous-1              30   4.8  
sp|Q99767|APBA2_HUMAN  Amyloid beta A4 precursor protein-bin...    30   8.2  
>sp|O95169|NIAM_HUMAN NADH-ubiquinone oxidoreductase ASHI subunit, mitochondrial
           precursor (Complex I-ASHI) (CI-ASHI)
          Length = 186

 Score = 98.6 bits (244), Expect = 1e-20
 Identities = 49/148 (33%), Positives = 76/148 (51%)
 Frame = +3

Query: 96  IQKLISRNINYYNYQWKPGPYPQNAEERAQAAKKYGLRPDDYMPYPDDGLAMGDYPYVKP 275
           +  L +R  ++      PGPYP+  EERA AAKKY +R +DY PYPDDG+  GDYP +  
Sbjct: 21  VMPLGARTASHMTKDMFPGPYPRTPEERAAAAKKYNMRVEDYEPYPDDGMGYGDYPKLPD 80

Query: 276 FNLDNRDPWEFYDYYPERRNYNEVLGIYNEAYRGGQSDPEPMTQFAPKVNWKMSLFYMTV 455
            +   RDPW  +D    R N+ E +  + + Y   + D  P       V+W +    +  
Sbjct: 81  RSQHERDPWYSWDQPGLRLNWGEPMHWHLDMYNRNRVDTSP-----TPVSWHVMCMQLFG 135

Query: 456 PFMVLCLLCYIDNRFKYFNPVMRKQFPY 539
               +  +C++ + +  + PV  KQ+PY
Sbjct: 136 FLAFMIFMCWVGDVYPVYQPVGPKQYPY 163
>sp|Q02372|NIAM_BOVIN NADH-ubiquinone oxidoreductase ASHI subunit, mitochondrial
           precursor (Complex I-ASHI) (CI-ASHI)
          Length = 186

 Score = 94.7 bits (234), Expect = 2e-19
 Identities = 51/158 (32%), Positives = 78/158 (49%)
 Frame = +3

Query: 66  LRIITKKNCKIQKLISRNINYYNYQWKPGPYPQNAEERAQAAKKYGLRPDDYMPYPDDGL 245
           +R + K    +  L +R  ++      PGPYP+  EERA AAKKY +R +DY PYPDDG 
Sbjct: 11  VRWLQKAARNVVPLGARTASHITKDMLPGPYPKTPEERAAAAKKYNMRVEDYEPYPDDGT 70

Query: 246 AMGDYPYVKPFNLDNRDPWEFYDYYPERRNYNEVLGIYNEAYRGGQSDPEPMTQFAPKVN 425
             GDYP +   +   RDPW  +D+   R N+ E +    + Y   + D  P       VN
Sbjct: 71  GYGDYPKLPDRSQQERDPWYDWDHPDLRLNWGEPMHWDLDMYIRNRVDTSP-----TPVN 125

Query: 426 WKMSLFYMTVPFMVLCLLCYIDNRFKYFNPVMRKQFPY 539
           W +   ++      +  + ++   +  + PV  KQ+PY
Sbjct: 126 WNLMCKHLFGFVAFMLFMFWVGETYPAYQPVGPKQYPY 163
>sp|Q9D6J5|NIAM_MOUSE NADH-ubiquinone oxidoreductase ASHI subunit, mitochondrial
           precursor (Complex I-ASHI) (CI-ASHI)
          Length = 186

 Score = 89.7 bits (221), Expect = 7e-18
 Identities = 50/133 (37%), Positives = 72/133 (54%), Gaps = 2/133 (1%)
 Frame = +3

Query: 147 PGPYPQNAEERAQAAKKYGLRPDDYMPYPDDGLAMGDYPYVKPFNLDNRDPWEFYDYYPE 326
           PG YP+  EERA AAKKY +R +DY PYPDDG+  GDYP +   +   RDPW  +D+   
Sbjct: 38  PGSYPRTPEERAAAAKKYNMRVEDYEPYPDDGMGYGDYPMLPNRSQHERDPWYQWDHSEL 97

Query: 327 RRNYNEVLGIYNEAYRGGQSDPEPMTQFAPKVNWKMSLFYM--TVPFMVLCLLCYIDNRF 500
           R N+ E +    + Y   + D  P       V+W +   ++   V FMV   + ++ + F
Sbjct: 98  RMNWGEPIHWDLDMYIRNRVDTSP-----TPVSWDVMCKHLFGFVAFMV--FMFWVGHVF 150

Query: 501 KYFNPVMRKQFPY 539
             + PV  KQ+PY
Sbjct: 151 PSYQPVGPKQYPY 163
>sp|Q04464|VGLB_SHV1 Glycoprotein B precursor
          Length = 920

 Score = 35.0 bits (79), Expect = 0.20
 Identities = 20/61 (32%), Positives = 30/61 (49%)
 Frame = +3

Query: 225 PYPDDGLAMGDYPYVKPFNLDNRDPWEFYDYYPERRNYNEVLGIYNEAYRGGQSDPEPMT 404
           PY +  LA GD+ YV PF     D    Y+ Y   R + +V G +      G++ PEP+ 
Sbjct: 294 PYSNFVLATGDFVYVSPFYGLGEDAHREYNAYSADR-FKQVDGFFPRDLDSGETAPEPVV 352

Query: 405 Q 407
           +
Sbjct: 353 R 353
>sp|Q25390|SCRA_LIMPO Alpha-scruin
          Length = 918

 Score = 33.1 bits (74), Expect = 0.74
 Identities = 14/38 (36%), Positives = 20/38 (52%)
 Frame = +3

Query: 291 RDPWEFYDYYPERRNYNEVLGIYNEAYRGGQSDPEPMT 404
           +D WEF+ Y P+ RNY+      +  Y  G  DP+  T
Sbjct: 618 KDRWEFFGYMPQPRNYHAAAYYRSAIYITGGYDPDVRT 655
>sp|P39352|YJHB_ECOLI Hypothetical metabolite transport protein yjhB
          Length = 405

 Score = 31.6 bits (70), Expect = 2.2
 Identities = 20/61 (32%), Positives = 27/61 (44%)
 Frame = +3

Query: 297 PWEFYDYYPERRNYNEVLGIYNEAYRGGQSDPEPMTQFAPKVNWKMSLFYMTVPFMVLCL 476
           P   YDY+P +        IYN    GG + P   T  +      +SLF +TV F  L +
Sbjct: 328 PKFIYDYFPTKLRGLGTGLIYNLGATGGMAAPVLATYISGYYGLGVSLFIVTVAFSALLI 387

Query: 477 L 479
           L
Sbjct: 388 L 388
>sp|Q8CPC7|TKT_STAES Transketolase (TK)
          Length = 662

 Score = 31.2 bits (69), Expect = 2.8
 Identities = 22/71 (30%), Positives = 31/71 (43%)
 Frame = +3

Query: 159 PQNAEERAQAAKKYGLRPDDYMPYPDDGLAMGDYPYVKPFNLDNRDPWEFYDYYPERRNY 338
           P   EERA   K+YGL P+     P+D   +     +K  N +N + W            
Sbjct: 264 PLGEEERALTFKEYGLDPEKRFNVPEDVYEIFKSTMLKRAN-ENEEAW------------ 310

Query: 339 NEVLGIYNEAY 371
           N +L  Y+EAY
Sbjct: 311 NNMLKNYSEAY 321
>sp|Q5HPJ9|TKT_STAEQ Transketolase
          Length = 662

 Score = 31.2 bits (69), Expect = 2.8
 Identities = 22/71 (30%), Positives = 31/71 (43%)
 Frame = +3

Query: 159 PQNAEERAQAAKKYGLRPDDYMPYPDDGLAMGDYPYVKPFNLDNRDPWEFYDYYPERRNY 338
           P   EERA   K+YGL P+     P+D   +     +K  N +N + W            
Sbjct: 264 PLGEEERALTFKEYGLDPEKRFNVPEDVYEIFKSTMLKRAN-ENEEAW------------ 310

Query: 339 NEVLGIYNEAY 371
           N +L  Y+EAY
Sbjct: 311 NNMLKNYSEAY 321
>sp|P26307|VIV1_MAIZE Regulatory protein viviparous-1
          Length = 691

 Score = 30.4 bits (67), Expect = 4.8
 Identities = 10/24 (41%), Positives = 15/24 (62%)
 Frame = +3

Query: 210 PDDYMPYPDDGLAMGDYPYVKPFN 281
           P  Y+P+P  G + G YP  +PF+
Sbjct: 335 PQQYVPFPPPGASTGSYPMPQPFS 358
>sp|Q99767|APBA2_HUMAN Amyloid beta A4 precursor protein-binding family A member 2
           (Neuron-specific X11L protein) (Neuronal
           Munc18-1-interacting protein 2) (Mint-2) (Adapter
           protein X11beta)
          Length = 749

 Score = 29.6 bits (65), Expect = 8.2
 Identities = 28/113 (24%), Positives = 44/113 (38%), Gaps = 12/113 (10%)
 Frame = +3

Query: 150 GPYPQNAEERAQAAKKYGLRPDDYMPYPDDGLAMGDYPYVKPFNLDNRDPWE-------- 305
           GP+P   E  A+ ++ Y   PD  +P P+D          +P  L+  D  E        
Sbjct: 146 GPHPHGHE--AEGSQDY---PDGQLPIPED----------EPSVLEAHDQEEDGHYCASK 190

Query: 306 --FYDYYPERRNYNEVLGIYNEAYRGG--QSDPEPMTQFAPKVNWKMSLFYMT 452
             + DYYPE  N N     Y      G  +   E + Q   ++   +S+  +T
Sbjct: 191 EGYQDYYPEEANGNTGASPYRLRRGDGDLEDQEEDIDQIVAEIKMSLSMTSIT 243
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 76,695,192
Number of Sequences: 369166
Number of extensions: 1616201
Number of successful extensions: 3590
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3489
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3587
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6219306880
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00326

  1. Dr_sW_016_J02
  2. Dr_sW_008_J13