Planaria EST Database

DrC_00259

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00259
         (674 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q8K3J1|NUIM_MOUSE  NADH-ubiquinone oxidoreductase 23 kDa ...   300   2e-81
sp|Q60HE3|NUIM_MACFA  NADH-ubiquinone oxidoreductase 23 kDa ...   299   4e-81
sp|O00217|NUIM_HUMAN  NADH-ubiquinone oxidoreductase 23 kDa ...   297   1e-80
sp|P42028|NUIM_BOVIN  NADH-ubiquinone oxidoreductase 23 kDa ...   295   7e-80
sp|Q22619|NUIM_CAEEL  NADH-ubiquinone oxidoreductase 23 kDa ...   288   7e-78
sp|Q12644|NUIM_NEUCR  NADH-ubiquinone oxidoreductase 23 kDa ...   263   2e-70
sp|Q42599|NUIM_ARATH  NADH-ubiquinone oxidoreductase 23 kDa ...   263   3e-70
sp|O21233|NUIM_RECAM  NADH-ubiquinone oxidoreductase subunit 8    261   1e-69
sp|P80269|NUIM_SOLTU  NADH-ubiquinone oxidoreductase 23 kDa ...   261   2e-69
sp|O24143|NUIM_TOBAC  NADH-ubiquinone oxidoreductase 23 kDa ...   258   8e-69

>sp|Q8K3J1|NUIM_MOUSE NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial
           precursor (Complex I-23KD) (CI-23KD) (TYKY subunit)
          Length = 212

 Score =  300 bits (769), Expect = 2e-81
 Identities = 141/177 (79%), Positives = 157/177 (88%), Gaps = 2/177 (1%)
 Frame = +2

Query: 86  YKTISKKGEATT--LKEAVDNGMNLYALTELFKGLGVTVGMIFREPATINYPFEKGPLSP 259
           YK ++KK + +   +K A DN   +   TEL +GLG+T+  +FREPATINYPFEKGPLSP
Sbjct: 36  YKYVNKKEQESEVDMKSATDNAARILMWTELIRGLGMTLSYLFREPATINYPFEKGPLSP 95

Query: 260 RFRGEHALRRYPSGEERCIACKLCEAICPAQAITIEAETRADGSRRTTRYDIDMTKCIFC 439
           RFRGEHALRRYPSGEERCIACKLCEAICPAQAITIEAE RADGSRRTTRYDIDMTKCI+C
Sbjct: 96  RFRGEHALRRYPSGEERCIACKLCEAICPAQAITIEAEPRADGSRRTTRYDIDMTKCIYC 155

Query: 440 GFCQEACPVDAIVEGPNFEYSTETHEELLYNKEKLLDNGDRWEPEIQANIEADYVYR 610
           GFCQEACPVDAIVEGPNFE+STETHEELLYNKEKLL+NGD+WE EI ANI+ADY+YR
Sbjct: 156 GFCQEACPVDAIVEGPNFEFSTETHEELLYNKEKLLNNGDKWEAEIAANIQADYLYR 212

>sp|Q60HE3|NUIM_MACFA NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial
           precursor (Complex I-23KD) (CI-23KD) (TYKY subunit)
          Length = 210

 Score =  299 bits (766), Expect = 4e-81
 Identities = 138/175 (78%), Positives = 153/175 (87%)
 Frame = +2

Query: 86  YKTISKKGEATTLKEAVDNGMNLYALTELFKGLGVTVGMIFREPATINYPFEKGPLSPRF 265
           YK ++ +   T +K   D        TELF+GLG+T+  +FREPATINYPFEKGPLSPRF
Sbjct: 36  YKYVNMQESKTDMKSVTDRAARTLLWTELFRGLGMTLSYLFREPATINYPFEKGPLSPRF 95

Query: 266 RGEHALRRYPSGEERCIACKLCEAICPAQAITIEAETRADGSRRTTRYDIDMTKCIFCGF 445
           RGEHALRRYPSGEERCIACKLCEA+CPAQAITIEAE RADGSRRTTRYDIDMTKCI+CGF
Sbjct: 96  RGEHALRRYPSGEERCIACKLCEAVCPAQAITIEAEPRADGSRRTTRYDIDMTKCIYCGF 155

Query: 446 CQEACPVDAIVEGPNFEYSTETHEELLYNKEKLLDNGDRWEPEIQANIEADYVYR 610
           CQEACPVDAIVEGPNFE+STETHEELLYNKEKLL+NGD+WE EI ANI+ADY+YR
Sbjct: 156 CQEACPVDAIVEGPNFEFSTETHEELLYNKEKLLNNGDKWEAEIAANIQADYLYR 210

>sp|O00217|NUIM_HUMAN NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial
           precursor (Complex I-23KD) (CI-23KD) (TYKY subunit)
          Length = 210

 Score =  297 bits (761), Expect = 1e-80
 Identities = 138/175 (78%), Positives = 152/175 (86%)
 Frame = +2

Query: 86  YKTISKKGEATTLKEAVDNGMNLYALTELFKGLGVTVGMIFREPATINYPFEKGPLSPRF 265
           YK ++ +     +K   D        TELF+GLG+T+  +FREPATINYPFEKGPLSPRF
Sbjct: 36  YKYVNMQDPEMDMKSVTDRAARTLLWTELFRGLGMTLSYLFREPATINYPFEKGPLSPRF 95

Query: 266 RGEHALRRYPSGEERCIACKLCEAICPAQAITIEAETRADGSRRTTRYDIDMTKCIFCGF 445
           RGEHALRRYPSGEERCIACKLCEAICPAQAITIEAE RADGSRRTTRYDIDMTKCI+CGF
Sbjct: 96  RGEHALRRYPSGEERCIACKLCEAICPAQAITIEAEPRADGSRRTTRYDIDMTKCIYCGF 155

Query: 446 CQEACPVDAIVEGPNFEYSTETHEELLYNKEKLLDNGDRWEPEIQANIEADYVYR 610
           CQEACPVDAIVEGPNFE+STETHEELLYNKEKLL+NGD+WE EI ANI+ADY+YR
Sbjct: 156 CQEACPVDAIVEGPNFEFSTETHEELLYNKEKLLNNGDKWEAEIAANIQADYLYR 210

>sp|P42028|NUIM_BOVIN NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial
           precursor (Complex I-23KD) (CI-23KD) (TYKY subunit)
          Length = 212

 Score =  295 bits (755), Expect = 7e-80
 Identities = 136/175 (77%), Positives = 152/175 (86%)
 Frame = +2

Query: 86  YKTISKKGEATTLKEAVDNGMNLYALTELFKGLGVTVGMIFREPATINYPFEKGPLSPRF 265
           YK ++ +  +  +K   D        TEL +GLG+T+  +FREPATINYPFEKGPLSPRF
Sbjct: 38  YKYVNLREPSMDMKSVTDRAAQTLLWTELIRGLGMTLSYLFREPATINYPFEKGPLSPRF 97

Query: 266 RGEHALRRYPSGEERCIACKLCEAICPAQAITIEAETRADGSRRTTRYDIDMTKCIFCGF 445
           RGEHALRRYPSGEERCIACKLCEA+CPAQAITIEAE RADGSRRTTRYDIDMTKCI+CGF
Sbjct: 98  RGEHALRRYPSGEERCIACKLCEAVCPAQAITIEAEPRADGSRRTTRYDIDMTKCIYCGF 157

Query: 446 CQEACPVDAIVEGPNFEYSTETHEELLYNKEKLLDNGDRWEPEIQANIEADYVYR 610
           CQEACPVDAIVEGPNFE+STETHEELLYNKEKLL+NGD+WE EI ANI+ADY+YR
Sbjct: 158 CQEACPVDAIVEGPNFEFSTETHEELLYNKEKLLNNGDKWEAEIAANIQADYLYR 212

>sp|Q22619|NUIM_CAEEL NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial
           precursor (Complex I-23KD) (CI-23KD)
          Length = 212

 Score =  288 bits (738), Expect = 7e-78
 Identities = 138/184 (75%), Positives = 155/184 (84%), Gaps = 1/184 (0%)
 Frame = +2

Query: 62  STQSKLNCYKTISKKGEAT-TLKEAVDNGMNLYALTELFKGLGVTVGMIFREPATINYPF 238
           +T  K + YK +    E   TL   ++ G++    TELF+G GV +G +F EPATINYPF
Sbjct: 29  NTIQKRSNYKFVGMPNETDGTLAGDLNYGLHNVFFTELFRGFGVMLGHVFMEPATINYPF 88

Query: 239 EKGPLSPRFRGEHALRRYPSGEERCIACKLCEAICPAQAITIEAETRADGSRRTTRYDID 418
           EKGPLS RFRGEHALRRYPSGEERCIACKLCEAICPAQAITIEAETR DGSRRTTRYDID
Sbjct: 89  EKGPLSSRFRGEHALRRYPSGEERCIACKLCEAICPAQAITIEAETRPDGSRRTTRYDID 148

Query: 419 MTKCIFCGFCQEACPVDAIVEGPNFEYSTETHEELLYNKEKLLDNGDRWEPEIQANIEAD 598
           MTKCI+CG CQEACPVDAIVEGPNFEYSTETHEELLYNKEKLL NGDRWEPE+ +N++A+
Sbjct: 149 MTKCIYCGLCQEACPVDAIVEGPNFEYSTETHEELLYNKEKLLLNGDRWEPELASNLQAE 208

Query: 599 YVYR 610
           Y+YR
Sbjct: 209 YLYR 212

>sp|Q12644|NUIM_NEUCR NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial
           precursor (Complex I-23KD) (CI-23KD)
          Length = 219

 Score =  263 bits (673), Expect = 2e-70
 Identities = 122/162 (75%), Positives = 136/162 (83%)
 Frame = +2

Query: 125 KEAVDNGMNLYALTELFKGLGVTVGMIFREPATINYPFEKGPLSPRFRGEHALRRYPSGE 304
           +  +D     + LTE+F+G+ V +   FR P TI YPFEKGP+SPRFRGEHALRRYPSGE
Sbjct: 58  EHVLDKNGRYFLLTEMFRGMYVAMEQFFRPPYTIYYPFEKGPISPRFRGEHALRRYPSGE 117

Query: 305 ERCIACKLCEAICPAQAITIEAETRADGSRRTTRYDIDMTKCIFCGFCQEACPVDAIVEG 484
           ERCIACKLCEA+CPAQAITIEAE RADGSRRTTRYDIDMTKCI+CGFCQE+CPVDAIVE 
Sbjct: 118 ERCIACKLCEAVCPAQAITIEAEERADGSRRTTRYDIDMTKCIYCGFCQESCPVDAIVES 177

Query: 485 PNFEYSTETHEELLYNKEKLLDNGDRWEPEIQANIEADYVYR 610
           PN EY+TET EELLYNKEKLL NGD+WEPE+ A I AD  YR
Sbjct: 178 PNAEYATETREELLYNKEKLLSNGDKWEPELAAAIRADSPYR 219

>sp|Q42599|NUIM_ARATH NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial
           precursor (Complex I-23KD) (CI-23KD) (Complex I-28.5KD)
           (CI-28.5KD)
          Length = 222

 Score =  263 bits (672), Expect = 3e-70
 Identities = 120/158 (75%), Positives = 137/158 (86%)
 Frame = +2

Query: 137 DNGMNLYALTELFKGLGVTVGMIFREPATINYPFEKGPLSPRFRGEHALRRYPSGEERCI 316
           +  +N   LTE+ +GL +T+   F    TINYPFEKGPLSPRFRGEHALRRYP+GEERCI
Sbjct: 65  ERSINTLFLTEMVRGLSLTLKYFFDPKVTINYPFEKGPLSPRFRGEHALRRYPTGEERCI 124

Query: 317 ACKLCEAICPAQAITIEAETRADGSRRTTRYDIDMTKCIFCGFCQEACPVDAIVEGPNFE 496
           ACKLCEA+CPAQAITIEAE R DGSRRTTRYDIDMTKCI+CGFCQEACPVDAIVEGPNFE
Sbjct: 125 ACKLCEAVCPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFE 184

Query: 497 YSTETHEELLYNKEKLLDNGDRWEPEIQANIEADYVYR 610
           ++TETHEELLY+KEKLL+NGDRWE EI  N+ ++ +YR
Sbjct: 185 FATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 222

>sp|O21233|NUIM_RECAM NADH-ubiquinone oxidoreductase subunit 8
          Length = 162

 Score =  261 bits (667), Expect = 1e-69
 Identities = 120/150 (80%), Positives = 134/150 (89%)
 Frame = +2

Query: 161 LTELFKGLGVTVGMIFREPATINYPFEKGPLSPRFRGEHALRRYPSGEERCIACKLCEAI 340
           LTEL KG+ +T+   FR+  T+NYPFEKGPLSPRFRGEHALRRY +GEERCIACKLCEAI
Sbjct: 13  LTELVKGMSLTLDYFFRKKVTLNYPFEKGPLSPRFRGEHALRRYQTGEERCIACKLCEAI 72

Query: 341 CPAQAITIEAETRADGSRRTTRYDIDMTKCIFCGFCQEACPVDAIVEGPNFEYSTETHEE 520
           CPAQAITIE+E R DGSRRTTRYDIDMTKCI+CGFCQEACPVDAIVEGPNFE++TETHEE
Sbjct: 73  CPAQAITIESEPRIDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFATETHEE 132

Query: 521 LLYNKEKLLDNGDRWEPEIQANIEADYVYR 610
           LLY+KEKLL NGDRWE EI AN+  + +YR
Sbjct: 133 LLYDKEKLLQNGDRWETEIAANLANEALYR 162

>sp|P80269|NUIM_SOLTU NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial
           precursor (Complex I-23KD) (CI-23KD) (Complex I-28.5KD)
           (CI-28.5KD)
          Length = 229

 Score =  261 bits (666), Expect = 2e-69
 Identities = 120/158 (75%), Positives = 137/158 (86%)
 Frame = +2

Query: 137 DNGMNLYALTELFKGLGVTVGMIFREPATINYPFEKGPLSPRFRGEHALRRYPSGEERCI 316
           +  +N   LTE+ +GL +T+   F +  TINYPFEKGPLSPRFRGEHALRRY +GEERCI
Sbjct: 72  ERSINTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEHALRRYATGEERCI 131

Query: 317 ACKLCEAICPAQAITIEAETRADGSRRTTRYDIDMTKCIFCGFCQEACPVDAIVEGPNFE 496
           ACKLCEAICPAQAITIEAE R DGSRRTTRYDIDMTKCI+CGFCQEACPVDAIVEGPNFE
Sbjct: 132 ACKLCEAICPAQAITIEAEEREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFE 191

Query: 497 YSTETHEELLYNKEKLLDNGDRWEPEIQANIEADYVYR 610
           ++TETHEELLY+KEKLL+NGDRWE EI  N+ ++ +YR
Sbjct: 192 FATETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 229

>sp|O24143|NUIM_TOBAC NADH-ubiquinone oxidoreductase 23 kDa subunit, mitochondrial
           precursor (Complex I-23KD) (CI-23KD) (Complex I-28.5KD)
           (CI-28.5KD)
          Length = 230

 Score =  258 bits (660), Expect = 8e-69
 Identities = 119/155 (76%), Positives = 135/155 (87%)
 Frame = +2

Query: 146 MNLYALTELFKGLGVTVGMIFREPATINYPFEKGPLSPRFRGEHALRRYPSGEERCIACK 325
           +N   LTE+ +GL +T+   F +  TINYPFEKGPLSPRFRGE  LRRYP+GEERCIACK
Sbjct: 76  INTLFLTEMVRGLMLTLKYFFEKKVTINYPFEKGPLSPRFRGEQPLRRYPTGEERCIACK 135

Query: 326 LCEAICPAQAITIEAETRADGSRRTTRYDIDMTKCIFCGFCQEACPVDAIVEGPNFEYST 505
           LCEAICPAQAITIEAE R DGSRRTTRYDIDMTKCI+CGFCQEACPVDAIVEGPNFE++T
Sbjct: 136 LCEAICPAQAITIEAEAREDGSRRTTRYDIDMTKCIYCGFCQEACPVDAIVEGPNFEFAT 195

Query: 506 ETHEELLYNKEKLLDNGDRWEPEIQANIEADYVYR 610
           ETHEELLY+KEKLL+NGDRWE EI  N+ ++ +YR
Sbjct: 196 ETHEELLYDKEKLLENGDRWETEIAENLRSESLYR 230

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,915,650
Number of Sequences: 369166
Number of extensions: 1493742
Number of successful extensions: 4676
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3760
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4345
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5684835195
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00259

  1. Dr_sW_007_F06
  2. Dr_sW_005_C17