Planaria EST Database

DrC_00233

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00233
         (828 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q92122|KPYK_XENLA  Pyruvate kinase, muscle isozyme (Cytos...   254   2e-67
sp|O62619|KPYK_DROME  Pyruvate kinase (PK)                        250   4e-66
sp|P14618|KPYM_HUMAN  Pyruvate kinase, isozymes M1/M2 (Pyruv...   249   7e-66
sp|P11980|KPYM_RAT  Pyruvate kinase, isozymes M1/M2 (Pyruvat...   247   3e-65
sp|P30613|KPYR_HUMAN  Pyruvate kinase, isozymes R/L (R-type/...   244   3e-64
sp|P52480|KPYM_MOUSE  Pyruvate kinase, isozyme M2                 243   4e-64
sp|P11974|KPYM_RABIT  Pyruvate kinase, isozymes M1/M2 (Pyruv...   243   6e-64
sp|Q29536|KPYR_CANFA  Pyruvate kinase, isozyme R                  242   8e-64
sp|P11979|KPYM_FELCA  Pyruvate kinase, isozyme M1 (Pyruvate ...   241   2e-63
sp|P53657|KPYR_MOUSE  Pyruvate kinase, isozymes R/L (L-PK)        237   3e-62

>sp|Q92122|KPYK_XENLA Pyruvate kinase, muscle isozyme (Cytosolic thyroid hormone binding
           protein) (CTHBP)
          Length = 527

 Score =  254 bits (649), Expect = 2e-67
 Identities = 122/212 (57%), Positives = 159/212 (75%)
 Frame = +3

Query: 3   RVGKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVM 182
           R GKPVICATQMLESMI KP+PT+AE SDVANA+LDGADC+MLSGETAKG YPLEAV++ 
Sbjct: 315 RAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQ 374

Query: 183 HSICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSA 362
           H+I REAE ++F  + FE ++R      D T +TAV AVEA+  C + AIIV+T +GRSA
Sbjct: 375 HAIAREAEAAIFHRQLFEELRRVSPLTRDPTEATAVGAVEASFKCSSGAIIVLTKSGRSA 434

Query: 363 HLIAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAF 542
           HL+++YRP  PI++VTR+  TARQ HL+RG+ P+ Y E   + W ED+D R+++      
Sbjct: 435 HLLSRYRPRAPIISVTRNGQTARQAHLYRGIFPVLYREAVHEAWAEDVDSRVNFAMDIGK 494

Query: 543 RRGFIKLNDFVIVVTGWKAGAGSTNTLRVLRI 638
            RGF K  D VIV+TGW+ G+G TNT+RV+ +
Sbjct: 495 ARGFFKSGDVVIVLTGWRPGSGFTNTMRVVPV 526

>sp|O62619|KPYK_DROME Pyruvate kinase (PK)
          Length = 533

 Score =  250 bits (638), Expect = 4e-66
 Identities = 121/213 (56%), Positives = 156/213 (73%)
 Frame = +3

Query: 3   RVGKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVM 182
           + GKPVICATQMLESM+ KP+PT+AEISDVANA+LDGADCVMLSGETAKG+YPLE V  M
Sbjct: 322 KAGKPVICATQMLESMVKKPRPTRAEISDVANAVLDGADCVMLSGETAKGEYPLECVLTM 381

Query: 183 HSICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSA 362
              C+EAE +++    F  + R   G  D +H+ A+AAVEAA    A+AI+VITT+G+SA
Sbjct: 382 AKTCKEAEAALWHQNLFNDLVRGA-GTIDASHAAAIAAVEAATKAKASAIVVITTSGKSA 440

Query: 363 HLIAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAF 542
             ++KYRP CPI+ VTR   TARQ HL+RGL P+ Y E  + DW +D+D R+ +      
Sbjct: 441 FQVSKYRPRCPIIAVTRFAQTARQAHLYRGLVPLIYKEPGLGDWLKDVDVRVQFGLQVGK 500

Query: 543 RRGFIKLNDFVIVVTGWKAGAGSTNTLRVLRIE 641
           + GFIK  D V+VVTGWK G+G TNT+R++ +E
Sbjct: 501 KNGFIKTGDSVVVVTGWKQGSGFTNTIRIVTVE 533

>sp|P14618|KPYM_HUMAN Pyruvate kinase, isozymes M1/M2 (Pyruvate kinase muscle isozyme)
           (Cytosolic thyroid hormone-binding protein) (CTHBP)
           (THBP1)
          Length = 531

 Score =  249 bits (636), Expect = 7e-66
 Identities = 120/212 (56%), Positives = 157/212 (74%)
 Frame = +3

Query: 3   RVGKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVM 182
           R GKPVICATQMLESMI KP+PT+AE SDVANA+LDGADC+MLSGETAKG YPLEAV++ 
Sbjct: 319 RAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQ 378

Query: 183 HSICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSA 362
           H I REAE +++  + FE ++R     +D T +TAV AVEA+  C + AIIV+T +GRSA
Sbjct: 379 HLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSA 438

Query: 363 HLIAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAF 542
           H +A+YRP  PI+ VTR+P TARQ HL+RG+ P+   +   + W ED+D R+++      
Sbjct: 439 HQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGK 498

Query: 543 RRGFIKLNDFVIVVTGWKAGAGSTNTLRVLRI 638
            RGF K  D VIV+TGW+ G+G TNT+RV+ +
Sbjct: 499 ARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPV 530

>sp|P11980|KPYM_RAT Pyruvate kinase, isozymes M1/M2 (Pyruvate kinase muscle isozyme)
          Length = 531

 Score =  247 bits (630), Expect = 3e-65
 Identities = 120/212 (56%), Positives = 153/212 (72%)
 Frame = +3

Query: 3   RVGKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVM 182
           R GKPVICATQMLESMI KP+PT+AE SDVANA+LDGADC+MLSGETAKG YPLEAV++ 
Sbjct: 319 RAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQ 378

Query: 183 HSICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSA 362
           H I REAE +VF    FE + R     TD   + A+ +VEA+  C AAA+IV+T +GRSA
Sbjct: 379 HLIAREAEAAVFHRLLFEELARASSQSTDPLEAMAMGSVEASYKCLAAALIVLTESGRSA 438

Query: 363 HLIAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAF 542
           H +A+YRP  PI+ VTR+P TARQ HL+RG+ P+   +  +  W ED+D R++       
Sbjct: 439 HQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLDAWAEDVDLRVNLAMNVGK 498

Query: 543 RRGFIKLNDFVIVVTGWKAGAGSTNTLRVLRI 638
            RGF K  D VIV+TGW+ G+G TNT+RV+ +
Sbjct: 499 ARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPV 530

>sp|P30613|KPYR_HUMAN Pyruvate kinase, isozymes R/L (R-type/L-type pyruvate kinase) (Red
           cell/liver pyruvate kinase)
          Length = 574

 Score =  244 bits (622), Expect = 3e-64
 Identities = 119/210 (56%), Positives = 153/210 (72%)
 Frame = +3

Query: 9   GKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVMHS 188
           GKPV+CATQMLESMI KP+PT+AE SDVANA+LDGADC+MLSGETAKG +P+EAV++ H+
Sbjct: 364 GKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHA 423

Query: 189 ICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSAHL 368
           I REAE +V+  + FE ++R      D T  TA+ AVEAA  C AAAIIV+TTTGRSA L
Sbjct: 424 IAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQL 483

Query: 369 IAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAFRR 548
           +++YRP   ++ VTR    ARQ HL RG+ P+ Y E     W +D+DRR+ +       R
Sbjct: 484 LSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLR 543

Query: 549 GFIKLNDFVIVVTGWKAGAGSTNTLRVLRI 638
           GF+++ D VIVVTGW+ G+G TN +RVL I
Sbjct: 544 GFLRVGDLVIVVTGWRPGSGYTNIMRVLSI 573

>sp|P52480|KPYM_MOUSE Pyruvate kinase, isozyme M2
          Length = 531

 Score =  243 bits (621), Expect = 4e-64
 Identities = 117/212 (55%), Positives = 154/212 (72%)
 Frame = +3

Query: 3   RVGKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVM 182
           R GKPVIC+TQMLE MI KP+PT+AE SDVANA+LDGADC+MLSGETAKG YPLEAV++ 
Sbjct: 319 RAGKPVICSTQMLEIMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQ 378

Query: 183 HSICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSA 362
           H I REAE +++  + FE ++R     +D T + AV AVEA+  C + AIIV+T +GRSA
Sbjct: 379 HLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGAIIVLTKSGRSA 438

Query: 363 HLIAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAF 542
           H +A+YRP  PI+ VTR+P TARQ HL+RG+ P+   +  +  W ED+D R++       
Sbjct: 439 HQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGK 498

Query: 543 RRGFIKLNDFVIVVTGWKAGAGSTNTLRVLRI 638
            RGF K  D VIV+TGW+ G+G TNT+RV+ +
Sbjct: 499 ARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPV 530

>sp|P11974|KPYM_RABIT Pyruvate kinase, isozymes M1/M2 (Pyruvate kinase muscle isozyme)
          Length = 531

 Score =  243 bits (619), Expect = 6e-64
 Identities = 118/212 (55%), Positives = 154/212 (72%)
 Frame = +3

Query: 3   RVGKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVM 182
           R GKPVICATQMLESMI KP+PT+AE SDVANA+LDGADC+MLSGETAKG YPLEAV++ 
Sbjct: 319 RAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQ 378

Query: 183 HSICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSA 362
           H I REAE ++F  + FE + R+    TD   + A+ +VEA+  C AAA+IV+T +GRSA
Sbjct: 379 HLIAREAEAAMFHRKLFEELARSSSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSA 438

Query: 363 HLIAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAF 542
           H +A+YRP  PI+ VTR+  TARQ HL+RG+ P+   +   + W ED+D R++       
Sbjct: 439 HQVARYRPRAPIIAVTRNHQTARQAHLYRGIFPVVCKDPVQEAWAEDVDLRVNLAMNVGK 498

Query: 543 RRGFIKLNDFVIVVTGWKAGAGSTNTLRVLRI 638
            RGF K  D VIV+TGW+ G+G TNT+RV+ +
Sbjct: 499 ARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPV 530

>sp|Q29536|KPYR_CANFA Pyruvate kinase, isozyme R
          Length = 519

 Score =  242 bits (618), Expect = 8e-64
 Identities = 118/210 (56%), Positives = 153/210 (72%)
 Frame = +3

Query: 9   GKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVMHS 188
           GKPV+CATQMLESMI KP+PT+AE SDVANA+LDGADC+MLSGETAKG++P+EAV++ H 
Sbjct: 309 GKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGKFPVEAVKMQHR 368

Query: 189 ICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSAHL 368
           I REAE +V+  + FE ++R      D T  TA+A VEAA  C AAAIIV+T TGRSA L
Sbjct: 369 IAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIATVEAAFKCCAAAIIVLTKTGRSAQL 428

Query: 369 IAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAFRR 548
           +++YRP   ++ VTR    ARQ HL RG+ P+ Y+E     W +D+DRR+ +       R
Sbjct: 429 LSRYRPRAAVIAVTRSAQAARQAHLCRGVFPLLYSEPPEAIWADDVDRRVQFGIESGKLR 488

Query: 549 GFIKLNDFVIVVTGWKAGAGSTNTLRVLRI 638
           GF+++ D VIVVTGW+ G+G TN +RVL I
Sbjct: 489 GFLRVGDLVIVVTGWRPGSGYTNIMRVLSI 518

>sp|P11979|KPYM_FELCA Pyruvate kinase, isozyme M1 (Pyruvate kinase muscle isozyme)
          Length = 531

 Score =  241 bits (614), Expect = 2e-63
 Identities = 118/212 (55%), Positives = 153/212 (72%)
 Frame = +3

Query: 3   RVGKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVM 182
           R GKPVICATQMLESMI KP+PT+AE SDVANA+LDGADC+MLSGETAKG YPLEAV++ 
Sbjct: 319 RAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQ 378

Query: 183 HSICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSA 362
           H I REAE ++F  + FE + R     TD   + A+ +VEA+  C AAA+IV+T +GRSA
Sbjct: 379 HLIAREAEAAMFHRKLFEELVRGSSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSA 438

Query: 363 HLIAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAF 542
           H +A+YRP  PI+ VTR+  TARQ HL+RG+ P+   +   + W ED+D R++       
Sbjct: 439 HQVARYRPRAPIIAVTRNHQTARQAHLYRGIFPVVCKDPVQEAWAEDVDLRVNLAMNVGK 498

Query: 543 RRGFIKLNDFVIVVTGWKAGAGSTNTLRVLRI 638
            RGF K  D VIV+TGW+ G+G TNT+RV+ +
Sbjct: 499 ARGFFKHGDVVIVLTGWRPGSGFTNTMRVVPV 530

>sp|P53657|KPYR_MOUSE Pyruvate kinase, isozymes R/L (L-PK)
          Length = 574

 Score =  237 bits (604), Expect = 3e-62
 Identities = 116/210 (55%), Positives = 151/210 (71%)
 Frame = +3

Query: 9   GKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVMHS 188
           GKPV+CATQMLESMI K +PT+AE SDVANA+LDGADC+MLSGETAKG +P+EAV++ H+
Sbjct: 364 GKPVVCATQMLESMITKARPTRAETSDVANAVLDGADCIMLSGETAKGSFPVEAVKMQHA 423

Query: 189 ICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSAHL 368
           I REAE +V+  + FE ++R      D T  TA+ AVEA+  C AAAIIV+T TGRSA L
Sbjct: 424 IAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEASFKCCAAAIIVLTKTGRSAQL 483

Query: 369 IAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAFRR 548
           +++YRP   ++ VTR    ARQ HL RG+ P+ Y E     W +D+DRR+ +       R
Sbjct: 484 LSRYRPRAAVIAVTRSAQAARQVHLSRGVFPLLYREPPEAVWADDVDRRVQFGIESGKLR 543

Query: 549 GFIKLNDFVIVVTGWKAGAGSTNTLRVLRI 638
           GF+++ D VIVVTGW+ G+G TN +RVL I
Sbjct: 544 GFLRVGDLVIVVTGWRPGSGYTNIMRVLTI 573

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 90,272,777
Number of Sequences: 369166
Number of extensions: 1712785
Number of successful extensions: 5153
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4886
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5107
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8004331590
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00233

  1. Dr_sW_006_L20
  2. Dr_sW_008_N23