Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_006_L20
(808 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q92122|KPYK_XENLA Pyruvate kinase, muscle isozyme (Cytos... 254 2e-67
sp|O62619|KPYK_DROME Pyruvate kinase (PK) 250 4e-66
sp|P14618|KPYM_HUMAN Pyruvate kinase, isozymes M1/M2 (Pyruv... 249 6e-66
sp|P11980|KPYM_RAT Pyruvate kinase, isozymes M1/M2 (Pyruvat... 247 3e-65
sp|P30613|KPYR_HUMAN Pyruvate kinase, isozymes R/L (R-type/... 244 3e-64
sp|P52480|KPYM_MOUSE Pyruvate kinase, isozyme M2 243 3e-64
sp|P11974|KPYM_RABIT Pyruvate kinase, isozymes M1/M2 (Pyruv... 243 6e-64
sp|Q29536|KPYR_CANFA Pyruvate kinase, isozyme R 242 8e-64
sp|P11979|KPYM_FELCA Pyruvate kinase, isozyme M1 (Pyruvate ... 241 2e-63
sp|P53657|KPYR_MOUSE Pyruvate kinase, isozymes R/L (L-PK) 237 3e-62
>sp|Q92122|KPYK_XENLA Pyruvate kinase, muscle isozyme (Cytosolic thyroid hormone binding
protein) (CTHBP)
Length = 527
Score = 254 bits (649), Expect = 2e-67
Identities = 122/212 (57%), Positives = 159/212 (75%)
Frame = +3
Query: 3 RVGKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVM 182
R GKPVICATQMLESMI KP+PT+AE SDVANA+LDGADC+MLSGETAKG YPLEAV++
Sbjct: 315 RAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQ 374
Query: 183 HSICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSA 362
H+I REAE ++F + FE ++R D T +TAV AVEA+ C + AIIV+T +GRSA
Sbjct: 375 HAIAREAEAAIFHRQLFEELRRVSPLTRDPTEATAVGAVEASFKCSSGAIIVLTKSGRSA 434
Query: 363 HLIAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAF 542
HL+++YRP PI++VTR+ TARQ HL+RG+ P+ Y E + W ED+D R+++
Sbjct: 435 HLLSRYRPRAPIISVTRNGQTARQAHLYRGIFPVLYREAVHEAWAEDVDSRVNFAMDIGK 494
Query: 543 RRGFIKLNDFVIVVTGWKAGAGSTNTLRVLRI 638
RGF K D VIV+TGW+ G+G TNT+RV+ +
Sbjct: 495 ARGFFKSGDVVIVLTGWRPGSGFTNTMRVVPV 526
>sp|O62619|KPYK_DROME Pyruvate kinase (PK)
Length = 533
Score = 250 bits (638), Expect = 4e-66
Identities = 121/213 (56%), Positives = 156/213 (73%)
Frame = +3
Query: 3 RVGKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVM 182
+ GKPVICATQMLESM+ KP+PT+AEISDVANA+LDGADCVMLSGETAKG+YPLE V M
Sbjct: 322 KAGKPVICATQMLESMVKKPRPTRAEISDVANAVLDGADCVMLSGETAKGEYPLECVLTM 381
Query: 183 HSICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSA 362
C+EAE +++ F + R G D +H+ A+AAVEAA A+AI+VITT+G+SA
Sbjct: 382 AKTCKEAEAALWHQNLFNDLVRGA-GTIDASHAAAIAAVEAATKAKASAIVVITTSGKSA 440
Query: 363 HLIAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAF 542
++KYRP CPI+ VTR TARQ HL+RGL P+ Y E + DW +D+D R+ +
Sbjct: 441 FQVSKYRPRCPIIAVTRFAQTARQAHLYRGLVPLIYKEPGLGDWLKDVDVRVQFGLQVGK 500
Query: 543 RRGFIKLNDFVIVVTGWKAGAGSTNTLRVLRIE 641
+ GFIK D V+VVTGWK G+G TNT+R++ +E
Sbjct: 501 KNGFIKTGDSVVVVTGWKQGSGFTNTIRIVTVE 533
>sp|P14618|KPYM_HUMAN Pyruvate kinase, isozymes M1/M2 (Pyruvate kinase muscle isozyme)
(Cytosolic thyroid hormone-binding protein) (CTHBP)
(THBP1)
Length = 531
Score = 249 bits (636), Expect = 6e-66
Identities = 120/212 (56%), Positives = 157/212 (74%)
Frame = +3
Query: 3 RVGKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVM 182
R GKPVICATQMLESMI KP+PT+AE SDVANA+LDGADC+MLSGETAKG YPLEAV++
Sbjct: 319 RAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQ 378
Query: 183 HSICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSA 362
H I REAE +++ + FE ++R +D T +TAV AVEA+ C + AIIV+T +GRSA
Sbjct: 379 HLIAREAEAAIYHLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSA 438
Query: 363 HLIAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAF 542
H +A+YRP PI+ VTR+P TARQ HL+RG+ P+ + + W ED+D R+++
Sbjct: 439 HQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGK 498
Query: 543 RRGFIKLNDFVIVVTGWKAGAGSTNTLRVLRI 638
RGF K D VIV+TGW+ G+G TNT+RV+ +
Sbjct: 499 ARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPV 530
>sp|P11980|KPYM_RAT Pyruvate kinase, isozymes M1/M2 (Pyruvate kinase muscle isozyme)
Length = 531
Score = 247 bits (630), Expect = 3e-65
Identities = 120/212 (56%), Positives = 153/212 (72%)
Frame = +3
Query: 3 RVGKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVM 182
R GKPVICATQMLESMI KP+PT+AE SDVANA+LDGADC+MLSGETAKG YPLEAV++
Sbjct: 319 RAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQ 378
Query: 183 HSICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSA 362
H I REAE +VF FE + R TD + A+ +VEA+ C AAA+IV+T +GRSA
Sbjct: 379 HLIAREAEAAVFHRLLFEELARASSQSTDPLEAMAMGSVEASYKCLAAALIVLTESGRSA 438
Query: 363 HLIAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAF 542
H +A+YRP PI+ VTR+P TARQ HL+RG+ P+ + + W ED+D R++
Sbjct: 439 HQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLDAWAEDVDLRVNLAMNVGK 498
Query: 543 RRGFIKLNDFVIVVTGWKAGAGSTNTLRVLRI 638
RGF K D VIV+TGW+ G+G TNT+RV+ +
Sbjct: 499 ARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPV 530
>sp|P30613|KPYR_HUMAN Pyruvate kinase, isozymes R/L (R-type/L-type pyruvate kinase) (Red
cell/liver pyruvate kinase)
Length = 574
Score = 244 bits (622), Expect = 3e-64
Identities = 119/210 (56%), Positives = 153/210 (72%)
Frame = +3
Query: 9 GKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVMHS 188
GKPV+CATQMLESMI KP+PT+AE SDVANA+LDGADC+MLSGETAKG +P+EAV++ H+
Sbjct: 364 GKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGNFPVEAVKMQHA 423
Query: 189 ICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSAHL 368
I REAE +V+ + FE ++R D T TA+ AVEAA C AAAIIV+TTTGRSA L
Sbjct: 424 IAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLTTTGRSAQL 483
Query: 369 IAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAFRR 548
+++YRP ++ VTR ARQ HL RG+ P+ Y E W +D+DRR+ + R
Sbjct: 484 LSRYRPRAAVIAVTRSAQAARQVHLCRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLR 543
Query: 549 GFIKLNDFVIVVTGWKAGAGSTNTLRVLRI 638
GF+++ D VIVVTGW+ G+G TN +RVL I
Sbjct: 544 GFLRVGDLVIVVTGWRPGSGYTNIMRVLSI 573
>sp|P52480|KPYM_MOUSE Pyruvate kinase, isozyme M2
Length = 531
Score = 243 bits (621), Expect = 3e-64
Identities = 117/212 (55%), Positives = 154/212 (72%)
Frame = +3
Query: 3 RVGKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVM 182
R GKPVIC+TQMLE MI KP+PT+AE SDVANA+LDGADC+MLSGETAKG YPLEAV++
Sbjct: 319 RAGKPVICSTQMLEIMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQ 378
Query: 183 HSICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSA 362
H I REAE +++ + FE ++R +D T + AV AVEA+ C + AIIV+T +GRSA
Sbjct: 379 HLIAREAEAAIYHLQLFEELRRLAPITSDPTEAAAVGAVEASFKCCSGAIIVLTKSGRSA 438
Query: 363 HLIAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAF 542
H +A+YRP PI+ VTR+P TARQ HL+RG+ P+ + + W ED+D R++
Sbjct: 439 HQVARYRPRAPIIAVTRNPQTARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGK 498
Query: 543 RRGFIKLNDFVIVVTGWKAGAGSTNTLRVLRI 638
RGF K D VIV+TGW+ G+G TNT+RV+ +
Sbjct: 499 ARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPV 530
>sp|P11974|KPYM_RABIT Pyruvate kinase, isozymes M1/M2 (Pyruvate kinase muscle isozyme)
Length = 531
Score = 243 bits (619), Expect = 6e-64
Identities = 118/212 (55%), Positives = 154/212 (72%)
Frame = +3
Query: 3 RVGKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVM 182
R GKPVICATQMLESMI KP+PT+AE SDVANA+LDGADC+MLSGETAKG YPLEAV++
Sbjct: 319 RAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQ 378
Query: 183 HSICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSA 362
H I REAE ++F + FE + R+ TD + A+ +VEA+ C AAA+IV+T +GRSA
Sbjct: 379 HLIAREAEAAMFHRKLFEELARSSSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSA 438
Query: 363 HLIAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAF 542
H +A+YRP PI+ VTR+ TARQ HL+RG+ P+ + + W ED+D R++
Sbjct: 439 HQVARYRPRAPIIAVTRNHQTARQAHLYRGIFPVVCKDPVQEAWAEDVDLRVNLAMNVGK 498
Query: 543 RRGFIKLNDFVIVVTGWKAGAGSTNTLRVLRI 638
RGF K D VIV+TGW+ G+G TNT+RV+ +
Sbjct: 499 ARGFFKKGDVVIVLTGWRPGSGFTNTMRVVPV 530
>sp|Q29536|KPYR_CANFA Pyruvate kinase, isozyme R
Length = 519
Score = 242 bits (618), Expect = 8e-64
Identities = 118/210 (56%), Positives = 153/210 (72%)
Frame = +3
Query: 9 GKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVMHS 188
GKPV+CATQMLESMI KP+PT+AE SDVANA+LDGADC+MLSGETAKG++P+EAV++ H
Sbjct: 309 GKPVVCATQMLESMITKPRPTRAETSDVANAVLDGADCIMLSGETAKGKFPVEAVKMQHR 368
Query: 189 ICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSAHL 368
I REAE +V+ + FE ++R D T TA+A VEAA C AAAIIV+T TGRSA L
Sbjct: 369 IAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIATVEAAFKCCAAAIIVLTKTGRSAQL 428
Query: 369 IAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAFRR 548
+++YRP ++ VTR ARQ HL RG+ P+ Y+E W +D+DRR+ + R
Sbjct: 429 LSRYRPRAAVIAVTRSAQAARQAHLCRGVFPLLYSEPPEAIWADDVDRRVQFGIESGKLR 488
Query: 549 GFIKLNDFVIVVTGWKAGAGSTNTLRVLRI 638
GF+++ D VIVVTGW+ G+G TN +RVL I
Sbjct: 489 GFLRVGDLVIVVTGWRPGSGYTNIMRVLSI 518
>sp|P11979|KPYM_FELCA Pyruvate kinase, isozyme M1 (Pyruvate kinase muscle isozyme)
Length = 531
Score = 241 bits (614), Expect = 2e-63
Identities = 118/212 (55%), Positives = 153/212 (72%)
Frame = +3
Query: 3 RVGKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVM 182
R GKPVICATQMLESMI KP+PT+AE SDVANA+LDGADC+MLSGETAKG YPLEAV++
Sbjct: 319 RAGKPVICATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQ 378
Query: 183 HSICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSA 362
H I REAE ++F + FE + R TD + A+ +VEA+ C AAA+IV+T +GRSA
Sbjct: 379 HLIAREAEAAMFHRKLFEELVRGSSHSTDLMEAMAMGSVEASYKCLAAALIVLTESGRSA 438
Query: 363 HLIAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAF 542
H +A+YRP PI+ VTR+ TARQ HL+RG+ P+ + + W ED+D R++
Sbjct: 439 HQVARYRPRAPIIAVTRNHQTARQAHLYRGIFPVVCKDPVQEAWAEDVDLRVNLAMNVGK 498
Query: 543 RRGFIKLNDFVIVVTGWKAGAGSTNTLRVLRI 638
RGF K D VIV+TGW+ G+G TNT+RV+ +
Sbjct: 499 ARGFFKHGDVVIVLTGWRPGSGFTNTMRVVPV 530
>sp|P53657|KPYR_MOUSE Pyruvate kinase, isozymes R/L (L-PK)
Length = 574
Score = 237 bits (604), Expect = 3e-62
Identities = 116/210 (55%), Positives = 151/210 (71%)
Frame = +3
Query: 9 GKPVICATQMLESMINKPKPTKAEISDVANAILDGADCVMLSGETAKGQYPLEAVQVMHS 188
GKPV+CATQMLESMI K +PT+AE SDVANA+LDGADC+MLSGETAKG +P+EAV++ H+
Sbjct: 364 GKPVVCATQMLESMITKARPTRAETSDVANAVLDGADCIMLSGETAKGSFPVEAVKMQHA 423
Query: 189 ICREAENSVFQSRFFEAIKRTIDGPTDQTHSTAVAAVEAAQGCHAAAIIVITTTGRSAHL 368
I REAE +V+ + FE ++R D T TA+ AVEA+ C AAAIIV+T TGRSA L
Sbjct: 424 IAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEASFKCCAAAIIVLTKTGRSAQL 483
Query: 369 IAKYRPTCPILTVTRHPITARQCHLWRGLHPIYYTEERIKDWTEDMDRRIHYTFTYAFRR 548
+++YRP ++ VTR ARQ HL RG+ P+ Y E W +D+DRR+ + R
Sbjct: 484 LSRYRPRAAVIAVTRSAQAARQVHLSRGVFPLLYREPPEAVWADDVDRRVQFGIESGKLR 543
Query: 549 GFIKLNDFVIVVTGWKAGAGSTNTLRVLRI 638
GF+++ D VIVVTGW+ G+G TN +RVL I
Sbjct: 544 GFLRVGDLVIVVTGWRPGSGYTNIMRVLTI 573
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 88,661,908
Number of Sequences: 369166
Number of extensions: 1691143
Number of successful extensions: 5059
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4804
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5013
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7666799535
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)