Planaria EST Database


DrC_00201

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00201
         (609 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q7ZW41|RPB7_BRARE  DNA-directed RNA polymerase II 19 kDa ...   191   1e-48
sp|P62488|RPB7_MOUSE  DNA-directed RNA polymerase II 19 kDa ...   191   2e-48
sp|P38421|RPB7_ARATH  DNA-directed RNA polymerase II 19 kDa ...   149   4e-36
sp|P46279|RPB7_SOYBN  DNA-directed RNA polymerase II 19 kDa ...   147   2e-35
sp|O14459|RPB7_SCHPO  DNA-directed RNA polymerase II 19 kDa ...   125   8e-29
sp|P34087|RPB7_YEAST  DNA-directed RNA polymerase II 19 kDa ...   108   1e-23
sp|Q57840|RPOE1_METJA  DNA-directed RNA polymerase subunit E'      57   5e-08
sp|P35718|RPC25_YEAST  DNA-directed RNA polymerase III 25 kD...    46   8e-05
sp|Q9Y535|RPC8_HUMAN  DNA-directed RNA polymerase III subuni...    39   0.010
sp|Q9D2C6|RPC8_MOUSE  DNA-directed RNA polymerase III subuni...    38   0.018
>sp|Q7ZW41|RPB7_BRARE DNA-directed RNA polymerase II 19 kDa polypeptide (RPB7)
          Length = 172

 Score =  191 bits (485), Expect = 1e-48
 Identities = 88/172 (51%), Positives = 119/172 (69%)
 Frame = +1

Query: 46  IFYHLNLKHEINLHPKYLGPNIIETIKTKLLSEVEGICTPNYGFIIAVISIDKIGDGVVQ 225
           +FYH++L+HEI LHP+Y GPN++ T+K KL +EVEG CT  YGF+IAV +ID IG GV+Q
Sbjct: 1   MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQ 60

Query: 226 QGRGFTLIPIEYKAIVFRQFVGEVLDAVVYNVTKIGIFAMAGAFQIFISVYAVPQNFQYD 405
            GRGF L P++YKAIVFR F GEV+DAVV  V K+G+F   G    FIS +++P   ++D
Sbjct: 61  PGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFD 120

Query: 406 CDTVPPCYKTAXXXXXXXXXXXXRVRIVGLRPDACGILGVGTLAEDYLGLIN 561
            ++ PPCYKT             R++IVG R D   I  +G+L +DYLGL++
Sbjct: 121 PNSNPPCYKTVDEDVVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDDYLGLVS 172
>sp|P62488|RPB7_MOUSE DNA-directed RNA polymerase II 19 kDa polypeptide (RPB7)
 sp|Q5E9B8|RPB7_BOVIN DNA-directed RNA polymerase II 19 kDa polypeptide (RPB7)
 sp|P62489|RPB7_RAT DNA-directed RNA polymerase II 19 kDa polypeptide (RPB7)
 sp|P62487|RPB7_HUMAN DNA-directed RNA polymerase II 19 kDa polypeptide (RPB7)
          Length = 172

 Score =  191 bits (484), Expect = 2e-48
 Identities = 88/172 (51%), Positives = 119/172 (69%)
 Frame = +1

Query: 46  IFYHLNLKHEINLHPKYLGPNIIETIKTKLLSEVEGICTPNYGFIIAVISIDKIGDGVVQ 225
           +FYH++L+HEI LHP+Y GPN++ T+K KL +EVEG CT  YGF+IAV +ID IG GV+Q
Sbjct: 1   MFYHISLEHEILLHPRYFGPNLLNTVKQKLFTEVEGTCTGKYGFVIAVTTIDNIGAGVIQ 60

Query: 226 QGRGFTLIPIEYKAIVFRQFVGEVLDAVVYNVTKIGIFAMAGAFQIFISVYAVPQNFQYD 405
            GRGF L P++YKAIVFR F GEV+DAVV  V K+G+F   G    FIS +++P   ++D
Sbjct: 61  PGRGFVLYPVKYKAIVFRPFKGEVVDAVVTQVNKVGLFTEIGPMSCFISRHSIPSEMEFD 120

Query: 406 CDTVPPCYKTAXXXXXXXXXXXXRVRIVGLRPDACGILGVGTLAEDYLGLIN 561
            ++ PPCYKT             R++IVG R D   I  +G+L +DYLGL++
Sbjct: 121 PNSNPPCYKTMDEDIVIQQDDEIRLKIVGTRVDKNDIFAIGSLMDDYLGLVS 172
>sp|P38421|RPB7_ARATH DNA-directed RNA polymerase II 19 kDa polypeptide (RNA polymerase
           II subunit 5)
          Length = 176

 Score =  149 bits (377), Expect = 4e-36
 Identities = 68/172 (39%), Positives = 110/172 (63%)
 Frame = +1

Query: 46  IFYHLNLKHEINLHPKYLGPNIIETIKTKLLSEVEGICTPNYGFIIAVISIDKIGDGVVQ 225
           +F+H+ L+  + LHP++ G N+ E + +KL+ +VEG C+  +GF++A+  ID IG G+++
Sbjct: 1   MFFHIVLERNMQLHPRFFGRNLKENLVSKLMKDVEGTCSGRHGFVVAITGIDTIGKGLIR 60

Query: 226 QGRGFTLIPIEYKAIVFRQFVGEVLDAVVYNVTKIGIFAMAGAFQIFISVYAVPQNFQYD 405
            G GF   P++Y+ +VFR F GE+L+AVV  V K+G FA AG  QIF+S + +P + ++ 
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTLVNKMGFFAEAGPVQIFVSKHLIPDDMEFQ 120

Query: 406 CDTVPPCYKTAXXXXXXXXXXXXRVRIVGLRPDACGILGVGTLAEDYLGLIN 561
              +P  Y T+            R++I+G R DA  I  VGT+ +D+LG+IN
Sbjct: 121 AGDMPN-YTTSDGSVKIQKECEVRLKIIGTRVDATAIFCVGTIKDDFLGVIN 171
>sp|P46279|RPB7_SOYBN DNA-directed RNA polymerase II 19 kDa polypeptide (RNA polymerase
           II subunit 5)
          Length = 176

 Score =  147 bits (372), Expect = 2e-35
 Identities = 67/172 (38%), Positives = 110/172 (63%)
 Frame = +1

Query: 46  IFYHLNLKHEINLHPKYLGPNIIETIKTKLLSEVEGICTPNYGFIIAVISIDKIGDGVVQ 225
           +F+H+ L+  + LHP+Y G N+ + + +KL+ +VEG C+  +GF++AV  I+ IG G+++
Sbjct: 1   MFFHIVLERNMQLHPRYFGRNLRDNLVSKLMKDVEGTCSGRHGFVVAVTGIENIGKGLIR 60

Query: 226 QGRGFTLIPIEYKAIVFRQFVGEVLDAVVYNVTKIGIFAMAGAFQIFISVYAVPQNFQYD 405
            G GF   P++Y+ +VFR F GE+L+AVV  V K+G FA AG  QIF+S + +P + ++ 
Sbjct: 61  DGTGFVTFPVKYQCVVFRPFKGEILEAVVTMVNKMGFFAEAGPVQIFVSNHLIPDDMEFQ 120

Query: 406 CDTVPPCYKTAXXXXXXXXXXXXRVRIVGLRPDACGILGVGTLAEDYLGLIN 561
              +P  Y T+            R++I+G R DA  I  +GT+ +D+LG+IN
Sbjct: 121 SGDMPN-YTTSDGSVKIQKDSEVRLKIIGTRVDATEIFCIGTIKDDFLGVIN 171
>sp|O14459|RPB7_SCHPO DNA-directed RNA polymerase II 19 kDa polypeptide
          Length = 172

 Score =  125 bits (314), Expect = 8e-29
 Identities = 66/172 (38%), Positives = 100/172 (58%), Gaps = 2/172 (1%)
 Frame = +1

Query: 49  FYHLNLKHEINLHPKYLGPNIIETIKTKLLSEVEGICTPNYGFIIAVISIDKIG--DGVV 222
           F+   L   I+LHP Y GP + + +K KLL++VEG C+  YG+II V+  + I    G V
Sbjct: 3   FFLKELSLTISLHPSYFGPRMQDYLKAKLLADVEGTCSGQYGYIICVLDSNTIDIDKGRV 62

Query: 223 QQGRGFTLIPIEYKAIVFRQFVGEVLDAVVYNVTKIGIFAMAGAFQIFISVYAVPQNFQY 402
             G+GF    ++Y+A+++R F GEV+DA+V  V K+G FA  G   +F+S + VP + ++
Sbjct: 63  VPGQGFAEFEVKYRAVLWRPFRGEVVDAIVTTVNKMGFFANIGPLNVFVSSHLVPPDMKF 122

Query: 403 DCDTVPPCYKTAXXXXXXXXXXXXRVRIVGLRPDACGILGVGTLAEDYLGLI 558
           D    PP Y  +            R++IVG R DA  I  + T+ EDYLG++
Sbjct: 123 DPTANPPNY--SGEDQVIEKGSNVRLKIVGTRTDATEIFAIATMKEDYLGVL 172
>sp|P34087|RPB7_YEAST DNA-directed RNA polymerase II 19 kDa polypeptide (B16)
          Length = 171

 Score =  108 bits (270), Expect = 1e-23
 Identities = 58/176 (32%), Positives = 97/176 (55%), Gaps = 5/176 (2%)
 Frame = +1

Query: 46  IFYHLNLKHEINLHPKYLGPNIIETIKTKLLSEVEGICTPNYGFIIAVISIDKIGDGVVQ 225
           +F+  +L   I LHP + GP + + +KTKLL EVEG CT  +G+I+ V+  D I    +Q
Sbjct: 1   MFFIKDLSLNITLHPSFFGPRMKQYLKTKLLEEVEGSCTGKFGYILCVLDYDNID---IQ 57

Query: 226 QGR-----GFTLIPIEYKAIVFRQFVGEVLDAVVYNVTKIGIFAMAGAFQIFISVYAVPQ 390
           +GR     G     ++Y+A+VF+ F GEV+D  V + ++ G     G  ++F++ + +PQ
Sbjct: 58  RGRILPTDGSAEFNVKYRAVVFKPFKGEVVDGTVVSCSQHGFEVQVGPMKVFVTKHLMPQ 117

Query: 391 NFQYDCDTVPPCYKTAXXXXXXXXXXXXRVRIVGLRPDACGILGVGTLAEDYLGLI 558
           +  ++  + PP Y+++            RV+I G       I  +G++ EDYLG I
Sbjct: 118 DLTFNAGSNPPSYQSS--EDVITIKSRIRVKIEGCISQVSSIHAIGSIKEDYLGAI 171
>sp|Q57840|RPOE1_METJA DNA-directed RNA polymerase subunit E'
          Length = 187

 Score = 56.6 bits (135), Expect = 5e-08
 Identities = 29/117 (24%), Positives = 60/117 (51%), Gaps = 1/117 (0%)
 Frame = +1

Query: 58  LNLKHEINLHPKYLGPNIIETIKTKLLSEVEGICTPNYGFIIAVISIDKIGDGVVQQGRG 237
           L +   + + P+  G ++ ET+K  L+ + EG    + GF+++++ +  IG+G V  G G
Sbjct: 5   LEIADVVKVPPEEFGKDLKETVKKILMEKYEGRLDKDVGFVLSIVDVKDIGEGKVVHGDG 64

Query: 238 FTLIPIEYKAIVFRQFVGEVLDAVVYNVTKIGIFAMAGAFQIFISVYAVPQNF-QYD 405
               P+ ++ +V+   + E+++  V +V + G F   G     I V  +  ++  YD
Sbjct: 65  SAYHPVVFETLVYIPEMYELIEGEVVDVVEFGSFVRLGPLDGLIHVSQIMDDYVSYD 121
>sp|P35718|RPC25_YEAST DNA-directed RNA polymerase III 25 kDa polypeptide (C25)
          Length = 212

 Score = 45.8 bits (107), Expect = 8e-05
 Identities = 29/120 (24%), Positives = 56/120 (46%)
 Frame = +1

Query: 76  INLHPKYLGPNIIETIKTKLLSEVEGICTPNYGFIIAVISIDKIGDGVVQQGRGFTLIPI 255
           + + P     + I  I  +L ++      PN G  I +  +  + +G ++ G G + I +
Sbjct: 11  VRIPPDQFHRDTISAITHQLNNKFANKIIPNVGLCITIYDLLTVEEGQLKPGDGSSYINV 70

Query: 256 EYKAIVFRQFVGEVLDAVVYNVTKIGIFAMAGAFQIFISVYAVPQNFQYDCDTVPPCYKT 435
            ++A+VF+ F+GE++   +   T  GI        IF  ++ +PQN  ++      CY T
Sbjct: 71  TFRAVVFKPFLGEIVTGWISKCTAEGI--KVSLLGIFDDIF-IPQNMLFE-----GCYYT 122
>sp|Q9Y535|RPC8_HUMAN DNA-directed RNA polymerase III subunit 22.9 kDa polypeptide (RPC8)
          Length = 204

 Score = 38.9 bits (89), Expect = 0.010
 Identities = 27/104 (25%), Positives = 47/104 (45%), Gaps = 1/104 (0%)
 Frame = +1

Query: 97  LGPNIIETIKTKLLSEVEGICTPNYGFIIAVISIDKIGDGVVQQGRGFTLIPIEYKAIVF 276
           L  +I E +  KL ++V      N G  I +  I K+ D  V  G G +   + ++ +VF
Sbjct: 22  LNDSIAEELNKKLANKV----VYNVGLCICLFDITKLEDAYVFPGDGASHTKVHFRCVVF 77

Query: 277 RQFVGEVLDAVVYNVTKIGIFAMAGAF-QIFISVYAVPQNFQYD 405
             F+ E+L   +   +  G+    G F  I I   ++ Q  ++D
Sbjct: 78  HPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFD 121
>sp|Q9D2C6|RPC8_MOUSE DNA-directed RNA polymerase III subunit 22.9 kDa polypeptide (RPC8)
          Length = 204

 Score = 38.1 bits (87), Expect = 0.018
 Identities = 27/104 (25%), Positives = 47/104 (45%), Gaps = 1/104 (0%)
 Frame = +1

Query: 97  LGPNIIETIKTKLLSEVEGICTPNYGFIIAVISIDKIGDGVVQQGRGFTLIPIEYKAIVF 276
           L  +I E +  KL ++V      N G  I +  I K+ D  V  G G +   + ++ +VF
Sbjct: 22  LNDSIAEELNKKLANKV----VYNVGLCICLFDITKLEDAYVFPGDGASHTKVHFRYVVF 77

Query: 277 RQFVGEVLDAVVYNVTKIGIFAMAGAF-QIFISVYAVPQNFQYD 405
             F+ E+L   +   +  G+    G F  I I   ++ Q  ++D
Sbjct: 78  HPFLDEILIGKIKGCSPEGVHVSLGFFDDILIPPESLQQPAKFD 121
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 62,397,011
Number of Sequences: 369166
Number of extensions: 1174950
Number of successful extensions: 2943
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2857
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2937
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4748907085
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00201

  1. Dr_sW_006_A07
  2. Dr_sW_023_K21