Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_03050
(844 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Con... 86 1e-16
sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Con... 84 5e-16
sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Con... 67 6e-11
sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1)... 61 3e-09
sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-... 59 2e-08
sp|P15285|PSPB_RABIT Pulmonary surfactant-associated protei... 53 9e-07
sp|P20097|SAP_CAVPO Saposin C (CO-beta-glucosidase) (Glucos... 53 1e-06
sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protei... 49 2e-05
sp|P07988|PSPB_HUMAN Pulmonary surfactant-associated protei... 48 3e-05
sp|P22355|PSPB_RAT Pulmonary surfactant-associated protein ... 47 5e-05
>sp|P26779|SAP_BOVIN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B (Sphingolipid activator protein 1) (SAP-1)
(Cerebroside sulfate activator) (CSAct) (Dispersin)
(Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 525
Score = 85.9 bits (211), Expect = 1e-16
Identities = 60/254 (23%), Positives = 109/254 (42%), Gaps = 33/254 (12%)
Frame = +1
Query: 37 CKKVPKVYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLCKSLEVKYGKEVNPLE-- 210
C ++ C ++++Y+ I+ + P EIC G C ++ K + P E
Sbjct: 231 CDRLGPGMSDMCKNYINQYSEVAIQMVMHMQP-KEICVLAGFCDEVKEMPMKTLVPAEVV 289
Query: 211 --------------------------CKACKLILHELIKEIGPDHSEETIKKALKIICKK 312
C+ C+ ++ E+ K I + +EE I AL +C K
Sbjct: 290 SENVIPALGLVEPIKKDPAPAKADIYCEVCEFVVKEVAKLIDNNRTEEEILHALDKVCSK 349
Query: 313 VPKVYHKKCDVFVDKYTNDIIKYIQENIPVDEICKKLGLCKSLEVKYGK-EVNPLE---- 477
+P ++C VD Y I+ + + + +C L LC S + V P +
Sbjct: 350 LPTSLAEQCQEVVDTYGRSILSILLDEASPELVCSMLHLCSSRGLPAATVRVMPRKDGGF 409
Query: 478 CKACKLILHELIKEIGPDHSEETIKKALKIICKKVPKVYHKKCDVFVDKYTNDIIKYIQK 657
C+ CK ++ L + + + ++E I AL+ C +P Y K+CD FV +Y +I+ + +
Sbjct: 410 CEVCKKLVGYLDRNLEKNSTKEQILAALEKGCSFLPDQYRKQCDQFVTEYEPVLIEILVE 469
Query: 658 NIPVDEICKKLGLC 699
+ +C K+G C
Sbjct: 470 VMDPSFVCLKIGAC 483
Score = 67.4 bits (163), Expect = 5e-11
Identities = 41/145 (28%), Positives = 69/145 (47%), Gaps = 5/145 (3%)
Frame = +1
Query: 13 IKKALKIICKKVPKVYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLCKSLEVKYGK 192
I AL +C K+P ++C VD Y I+ + + +C L LC S +
Sbjct: 339 ILHALDKVCSKLPTSLAEQCQEVVDTYGRSILSILLDEASPELVCSMLHLCSSRGLPAAT 398
Query: 193 -EVNPLE----CKACKLILHELIKEIGPDHSEETIKKALKIICKKVPKVYHKKCDVFVDK 357
V P + C+ CK ++ L + + + ++E I AL+ C +P Y K+CD FV +
Sbjct: 399 VRVMPRKDGGFCEVCKKLVGYLDRNLEKNSTKEQILAALEKGCSFLPDQYRKQCDQFVTE 458
Query: 358 YTNDIIKYIQENIPVDEICKKLGLC 432
Y +I+ + E + +C K+G C
Sbjct: 459 YEPVLIEILVEVMDPSFVCLKIGAC 483
Score = 55.1 bits (131), Expect = 2e-07
Identities = 62/303 (20%), Positives = 110/303 (36%), Gaps = 76/303 (25%)
Frame = +1
Query: 25 LKIICKKVPKV-YHKKCDVFVDKYTNDIIKYIQKNIP-VDEICKKLGLCKSLEVKYGKEV 198
L+ C +PK C VD Y I+ I+ + E+C L LC+SL+ +
Sbjct: 90 LERTCDWLPKPNMSASCKEIVDSYLPVILDMIKGQMSHPGEVCSALNLCESLQKHLAELN 149
Query: 199 NPLECKACKLILHELIKEIGP--------------DHSEETIKKALKIICKKVPK----- 321
+ + ++ ++ ++ + + P HS+ KKA +C+ +
Sbjct: 150 HQKQLESNQIPELDMAEVVAPFMANIPFLLYPQDGSHSKPQPKKANGNVCQDCIQLVTDV 209
Query: 322 --------------VYHKK-------------CDVFVDKYTNDIIKYIQENIPVDEICKK 420
V H K C ++++Y+ I+ + P EIC
Sbjct: 210 QEALRTNSTFVEALVDHAKEECDRLGPGMSDMCKNYINQYSEVAIQMVMHMQP-KEICVL 268
Query: 421 LGLCKSLEVKYGKEVNPLE----------------------------CKACKLILHELIK 516
G C ++ K + P E C+ C+ ++ E+ K
Sbjct: 269 AGFCDEVKEMPMKTLVPAEVVSENVIPALGLVEPIKKDPAPAKADIYCEVCEFVVKEVAK 328
Query: 517 EIGPDHSEETIKKALKIICKKVPKVYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGL 696
I + +EE I AL +C K+P ++C VD Y I+ + + +C L L
Sbjct: 329 LIDNNRTEEEILHALDKVCSKLPTSLAEQCQEVVDTYGRSILSILLDEASPELVCSMLHL 388
Query: 697 CKS 705
C S
Sbjct: 389 CSS 391
Score = 34.7 bits (78), Expect = 0.34
Identities = 27/93 (29%), Positives = 43/93 (46%), Gaps = 2/93 (2%)
Frame = +1
Query: 196 VNPLECKACKLILHELIKEIGPDHSEETIKKALKIICKKVPKV-YHKKCDVFVDKYTNDI 372
V L C CK ++ + + +E+ I L+ C +PK C VD Y I
Sbjct: 58 VKSLPCDICKDVITAAGNLLKDNATEQEILMYLERTCDWLPKPNMSASCKEIVDSYLPVI 117
Query: 373 IKYIQENIP-VDEICKKLGLCKSLEVKYGKEVN 468
+ I+ + E+C L LC+SL+ K+ E+N
Sbjct: 118 LDMIKGQMSHPGEVCSALNLCESLQ-KHLAELN 149
Score = 33.9 bits (76), Expect = 0.58
Identities = 24/85 (28%), Positives = 38/85 (44%), Gaps = 2/85 (2%)
Frame = +1
Query: 463 VNPLECKACKLILHELIKEIGPDHSEETIKKALKIICKKVPKV-YHKKCDVFVDKYTNDI 639
V L C CK ++ + + +E+ I L+ C +PK C VD Y I
Sbjct: 58 VKSLPCDICKDVITAAGNLLKDNATEQEILMYLERTCDWLPKPNMSASCKEIVDSYLPVI 117
Query: 640 IKYIQKNIP-VDEICKKLGLCKSLE 711
+ I+ + E+C L LC+SL+
Sbjct: 118 LDMIKGQMSHPGEVCSALNLCESLQ 142
>sp|P07602|SAP_HUMAN Proactivator polypeptide precursor [Contains: Saposin A (Protein
A); Saposin B-Val; Saposin B (Sphingolipid activator
protein 1) (SAP-1) (Cerebroside sulfate activator)
(CSAct) (Dispersin) (Sulfatide/GM1 activator); Saposin C
(Co-beta-glucosidase) (A1 activator) (Glucosylceramidase
activator) (Sphingolipid activator protein 2) (SAP-2);
Saposin D (Protein C) (Component C)]
Length = 524
Score = 84.0 bits (206), Expect = 5e-16
Identities = 46/170 (27%), Positives = 87/170 (51%), Gaps = 5/170 (2%)
Frame = +1
Query: 211 CKACKLILHELIKEIGPDHSEETIKKALKIICKKVPKVYHKKCDVFVDKYTNDIIKYIQE 390
C+ C+ ++ E+ K I + +E+ I A +C K+PK ++C VD Y + I+ + E
Sbjct: 315 CEVCEFLVKEVTKLIDNNKTEKEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLE 374
Query: 391 NIPVDEICKKLGLCK-----SLEVKYGKEVNPLECKACKLILHELIKEIGPDHSEETIKK 555
+ + +C L LC +L V + + C+ CK ++ L + + + +++ I
Sbjct: 375 EVSPELVCSMLHLCSGTRLPALTVHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILA 434
Query: 556 ALKIICKKVPKVYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLCKS 705
AL+ C +P Y K+CD FV +Y +I+ + + + +C K+G C S
Sbjct: 435 ALEKGCSFLPDPYQKQCDQFVAEYEPVLIEILVEVMDPSFVCLKIGACPS 484
Score = 70.5 bits (171), Expect = 6e-12
Identities = 40/149 (26%), Positives = 74/149 (49%), Gaps = 5/149 (3%)
Frame = +1
Query: 7 RTIKKALKIICKKVPKVYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLCK-----S 171
+ I A +C K+PK ++C VD Y + I+ + + + + +C L LC +
Sbjct: 336 KEILDAFDKMCSKLPKSLSEECQEVVDTYGSSILSILLEEVSPELVCSMLHLCSGTRLPA 395
Query: 172 LEVKYGKEVNPLECKACKLILHELIKEIGPDHSEETIKKALKIICKKVPKVYHKKCDVFV 351
L V + + C+ CK ++ L + + + +++ I AL+ C +P Y K+CD FV
Sbjct: 396 LTVHVTQPKDGGFCEVCKKLVGYLDRNLEKNSTKQEILAALEKGCSFLPDPYQKQCDQFV 455
Query: 352 DKYTNDIIKYIQENIPVDEICKKLGLCKS 438
+Y +I+ + E + +C K+G C S
Sbjct: 456 AEYEPVLIEILVEVMDPSFVCLKIGACPS 484
Score = 35.4 bits (80), Expect = 0.20
Identities = 29/93 (31%), Positives = 43/93 (46%), Gaps = 2/93 (2%)
Frame = +1
Query: 196 VNPLECKACKLILHELIKEIGPDHSEETIKKALKIICKKVPKV-YHKKCDVFVDKYTNDI 372
V L C CK ++ + + +EE I L+ C +PK C VD Y I
Sbjct: 58 VKSLPCDICKDVVTAAGDMLKDNATEEEILVYLEKTCDWLPKPNMSASCKEIVDSYLPVI 117
Query: 373 IKYIQ-ENIPVDEICKKLGLCKSLEVKYGKEVN 468
+ I+ E E+C L LC+SL+ K+ E+N
Sbjct: 118 LDIIKGEMSRPGEVCSALNLCESLQ-KHLAELN 149
Score = 34.7 bits (78), Expect = 0.34
Identities = 30/111 (27%), Positives = 48/111 (43%), Gaps = 3/111 (2%)
Frame = +1
Query: 388 ENIPVDEICKKLGLCKSLEVKYGKE-VNPLECKACKLILHELIKEIGPDHSEETIKKALK 564
+N+ C + C L+ + K V L C CK ++ + + +EE I L+
Sbjct: 34 QNVKTASDCGAVKHC--LQTVWNKPTVKSLPCDICKDVVTAAGDMLKDNATEEEILVYLE 91
Query: 565 IICKKVPKV-YHKKCDVFVDKYTNDIIKYIQKNIP-VDEICKKLGLCKSLE 711
C +PK C VD Y I+ I+ + E+C L LC+SL+
Sbjct: 92 KTCDWLPKPNMSASCKEIVDSYLPVILDIIKGEMSRPGEVCSALNLCESLQ 142
>sp|O13035|SAP_CHICK Proactivator polypeptide precursor [Contains: Saposin A; Saposin B;
Saposin C; Saposin D]
Length = 518
Score = 67.0 bits (162), Expect = 6e-11
Identities = 49/248 (19%), Positives = 111/248 (44%), Gaps = 27/248 (10%)
Frame = +1
Query: 37 CKKVPKVYHKKCDVFVDKYTNDIIKYIQ--KNIPVDEICKKLGLCKSL-----------E 177
C ++ C ++ +Y++ I+ + K+ +IC +G C S+ +
Sbjct: 229 CDRLGPGMSDMCKSYISEYSDLAIQMMMHMKDQQPKDICAMVGFCPSVKSVPLQTLVPAQ 288
Query: 178 VKYGKEVNPLE-----------CKACKLILHELIKEIGPDHSEETIKKALKIICKKVPKV 324
V + ++ +E C+ C+ ++ E+ + + +EE I ++++C +P
Sbjct: 289 VVHEVKMETVEKATVQEKTFSVCEICETMVKEVTGLLESNKTEEEIVHEMEVVCYLLPAS 348
Query: 325 YHKKCDVFVDKYTNDIIKYIQENIPVDEICKKLGLCKSLEV-KYGKEVNPLE--CKACKL 495
+C F++ Y +I + E + +C L C + + + V P C CK+
Sbjct: 349 VKDQCKDFIEVYGQALIDMLLEATNPEAVCVMLKCCAANKPPQQPVVVKPAGGFCDICKM 408
Query: 496 ILHELIKEIGPDHSEETIKKALKIICKKVPKVYHKKCDVFVDKYTNDIIKYIQKNIPVDE 675
I+ KE+ + + I+ L+ +C +P+ +C FV++Y +++ + + +
Sbjct: 409 IVAYADKELEKNATTTEIEALLEKVCHFLPESVSDQCVQFVEQYEPVVVQLLAEMMDPTF 468
Query: 676 ICKKLGLC 699
+C KLG+C
Sbjct: 469 VCTKLGVC 476
Score = 53.5 bits (127), Expect = 7e-07
Identities = 31/139 (22%), Positives = 67/139 (48%), Gaps = 3/139 (2%)
Frame = +1
Query: 25 LKIICKKVPKVYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLCKSLEV-KYGKEVN 201
++++C +P +C F++ Y +I + + + +C L C + + + V
Sbjct: 338 MEVVCYLLPASVKDQCKDFIEVYGQALIDMLLEATNPEAVCVMLKCCAANKPPQQPVVVK 397
Query: 202 PLE--CKACKLILHELIKEIGPDHSEETIKKALKIICKKVPKVYHKKCDVFVDKYTNDII 375
P C CK+I+ KE+ + + I+ L+ +C +P+ +C FV++Y ++
Sbjct: 398 PAGGFCDICKMIVAYADKELEKNATTTEIEALLEKVCHFLPESVSDQCVQFVEQYEPVVV 457
Query: 376 KYIQENIPVDEICKKLGLC 432
+ + E + +C KLG+C
Sbjct: 458 QLLAEMMDPTFVCTKLGVC 476
Score = 32.3 bits (72), Expect = 1.7
Identities = 13/51 (25%), Positives = 29/51 (56%)
Frame = +1
Query: 13 IKKALKIICKKVPKVYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLC 165
I+ L+ +C +P+ +C FV++Y +++ + + + +C KLG+C
Sbjct: 426 IEALLEKVCHFLPESVSDQCVQFVEQYEPVVVQLLAEMMDPTFVCTKLGVC 476
>sp|P10960|SAP_RAT Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 554
Score = 61.2 bits (147), Expect = 3e-09
Identities = 49/233 (21%), Positives = 95/233 (40%), Gaps = 40/233 (17%)
Frame = +1
Query: 127 IPVDEICKKLGLCKSLEVKYGKEV----NPLECKACKLILHELIKEIGPDHSEETIKKAL 294
+P E K + L Y ++V N + C+ C+L++ +L + I + +EE + K L
Sbjct: 282 VPATEAIKNILPALELTDPYEQDVIQAQNVIFCQVCQLVMRKLSELIINNATEELLIKGL 341
Query: 295 KIICKKVPKVYHKKCDVFVDKYTNDIIKYIQENIPVDEICKKLGLCKSLEVKYG------ 456
C +P KC + + ++ + + + +C + LC + G
Sbjct: 342 SKACSLLPAPASTKCQEVLVTFGPSLLDVLMHEVNPNFLCGVISLCSANPNLVGTLEQPA 401
Query: 457 --------KEVNPLE----------------------CKACKLILHELIKEIGPDHSEET 546
KE P + C+ CK ++ L + + ++E
Sbjct: 402 AAIVSALPKEPAPPKQPEEPKQSALRAHVPPQKNGGFCEVCKKLVIYLEHNLEKNSTKEE 461
Query: 547 IKKALKIICKKVPKVYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLCKS 705
I AL+ C +P Y K+CD FV +Y +++ + + + +C K+G+C S
Sbjct: 462 ILAALEKGCSFLPDPYQKQCDEFVAEYEPLLLEILVEVMDPSFVCSKIGVCPS 514
Score = 37.0 bits (84), Expect = 0.068
Identities = 17/53 (32%), Positives = 30/53 (56%)
Frame = +1
Query: 13 IKKALKIICKKVPKVYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLCKS 171
I AL+ C +P Y K+CD FV +Y +++ + + + +C K+G+C S
Sbjct: 462 ILAALEKGCSFLPDPYQKQCDEFVAEYEPLLLEILVEVMDPSFVCSKIGVCPS 514
Score = 32.3 bits (72), Expect = 1.7
Identities = 23/82 (28%), Positives = 36/82 (43%), Gaps = 2/82 (2%)
Frame = +1
Query: 472 LECKACKLILHELIKEIGPDHSEETIKKALKIICKKV-PKVYHKKCDVFVDKYTNDIIKY 648
L C CK ++ E + + +EE I L+ C + C VD Y I+
Sbjct: 61 LPCDICKTVVTEAGNLLKDNATEEEILHYLEKTCAWIHDSSLSASCKEVVDSYLPVILDM 120
Query: 649 IQKNIP-VDEICKKLGLCKSLE 711
I+ + E+C L LC+SL+
Sbjct: 121 IKGEMSNPGEVCSALNLCQSLQ 142
>sp|Q61207|SAP_MOUSE Sulfated glycoprotein 1 precursor (SGP-1) (Prosaposin)
Length = 557
Score = 58.9 bits (141), Expect = 2e-08
Identities = 41/205 (20%), Positives = 81/205 (39%), Gaps = 36/205 (17%)
Frame = +1
Query: 199 NPLECKACKLILHELIKEIGPDHSEETIKKALKIICKKVPKVYHKKCDVFVDKYTNDIIK 378
N + C+ C+ ++++ + I + +EE + K L C +P KC V + ++
Sbjct: 313 NVILCQTCQFVMNKFSELIVNNATEELLVKGLSNACALLPDPARTKCQEVVGTFGPSLLD 372
Query: 379 YIQENIPVDEICKKLGLC------------------------------------KSLEVK 450
+ +C +GLC +L
Sbjct: 373 IFIHEVNPSSLCGVIGLCAARPELVEALEQPAPAIVSALLKEPTPPKQPAQPKQSALPAH 432
Query: 451 YGKEVNPLECKACKLILHELIKEIGPDHSEETIKKALKIICKKVPKVYHKKCDVFVDKYT 630
+ N C+ CK ++ L + + ++E I AL+ C +P Y K+CD FV +Y
Sbjct: 433 VPPQKNGGFCEVCKKLVLYLEHNLEKNSTKEEILAALEKGCSFLPDPYQKQCDDFVAEYE 492
Query: 631 NDIIKYIQKNIPVDEICKKLGLCKS 705
+++ + + + +C K+G+C S
Sbjct: 493 PLLLEILVEVMDPGFVCSKIGVCPS 517
Score = 36.2 bits (82), Expect = 0.12
Identities = 17/53 (32%), Positives = 30/53 (56%)
Frame = +1
Query: 13 IKKALKIICKKVPKVYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLCKS 171
I AL+ C +P Y K+CD FV +Y +++ + + + +C K+G+C S
Sbjct: 465 ILAALEKGCSFLPDPYQKQCDDFVAEYEPLLLEILVEVMDPGFVCSKIGVCPS 517
Score = 32.0 bits (71), Expect = 2.2
Identities = 22/82 (26%), Positives = 37/82 (45%), Gaps = 2/82 (2%)
Frame = +1
Query: 472 LECKACKLILHELIKEIGPDHSEETIKKALKIICKKV-PKVYHKKCDVFVDKYTNDIIKY 648
L C CK ++ E + + ++E I L+ C+ + C VD Y I+
Sbjct: 61 LPCDICKTVVTEAGNLLKDNATQEEILHYLEKTCEWIHDSSLSASCKEVVDSYLPVILDM 120
Query: 649 IQKNIP-VDEICKKLGLCKSLE 711
I+ + E+C L LC+SL+
Sbjct: 121 IKGEMSNPGEVCSALNLCQSLQ 142
>sp|P15285|PSPB_RABIT Pulmonary surfactant-associated protein B precursor (SP-B) (6 kDa
protein) (Pulmonary surfactant-associated proteolipid
SPL(Phe))
Length = 370
Score = 53.1 bits (126), Expect = 9e-07
Identities = 59/270 (21%), Positives = 106/270 (39%), Gaps = 40/270 (14%)
Frame = +1
Query: 10 TIKKALKIICKKVP-KVYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLCK------ 168
TI+K L+ C +P K+ +C +D Y I Y Q I IC+ LGLC+
Sbjct: 92 TIRKFLEHECDVLPLKLLVPQCHHVLDVYFPLTITYFQSQINAKAICQHLGLCQPGSPEP 151
Query: 169 ----------------SLEVKYGKEVNPLECKACKLIL------HELIKEIGPDHSEETI 282
+L K G L + + L L+K I + +
Sbjct: 152 PLDPLPDKLVLPTLLGALPAKPGPHTQDLSAQRFPIPLPLCWLCRTLLKRIQAMIPKGVL 211
Query: 283 KKALKIICKKVPKVYHKKCDVFVDKYTNDIIKYIQENIPVDEICKKLGLCKSLE----VK 450
A+ +C VP V C ++YT +++ + ++ +C + C S++ V
Sbjct: 212 AMAVAQVCHVVPLVVGGICQCLAERYTVILLEVLLGHVLPQLVCGLVLRCSSVDSIGQVP 271
Query: 451 YGKEVNP-------LECKACKLILHELIKEIGPDHSEETIKKALKIICKKVPKVYHKKCD 609
E P EC+ C + + + SE+T +A+ C ++ ++C+
Sbjct: 272 PTLEALPGEWLPQDPECRLCMSVTTQ-----ARNISEQTRPQAVYHACLS-SQLDKQECE 325
Query: 610 VFVDKYTNDIIKYIQKNIPVDEICKKLGLC 699
FV+ +T ++ + + IC+ LG C
Sbjct: 326 QFVELHTPQLLSLLSRGWDARAICQALGAC 355
Score = 50.8 bits (120), Expect = 5e-06
Identities = 51/218 (23%), Positives = 87/218 (39%), Gaps = 29/218 (13%)
Frame = +1
Query: 145 CKKLGLCKSLEVKYGKEVNPLECKACKLILHELIKEIGPDHSEETIKKALKIICKKVP-K 321
CK LG C L+ +G C+ C+ I++ L K ++TI+K L+ C +P K
Sbjct: 50 CKALGHC--LQEVWGHVGADDLCQECQDIVNILTKMTKEAIFQDTIRKFLEHECDVLPLK 107
Query: 322 VYHKKCDVFVDKYTNDIIKYIQENIPVDEICKKLGLCK---------------------- 435
+ +C +D Y I Y Q I IC+ LGLC+
Sbjct: 108 LLVPQCHHVLDVYFPLTITYFQSQINAKAICQHLGLCQPGSPEPPLDPLPDKLVLPTLLG 167
Query: 436 SLEVKYGKEVNPLECKACKLIL------HELIKEIGPDHSEETIKKALKIICKKVPKVYH 597
+L K G L + + L L+K I + + A+ +C VP V
Sbjct: 168 ALPAKPGPHTQDLSAQRFPIPLPLCWLCRTLLKRIQAMIPKGVLAMAVAQVCHVVPLVVG 227
Query: 598 KKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLCKSLE 711
C ++YT +++ + ++ +C + C S++
Sbjct: 228 GICQCLAERYTVILLEVLLGHVLPQLVCGLVLRCSSVD 265
>sp|P20097|SAP_CAVPO Saposin C (CO-beta-glucosidase) (Glucosylceramidase activator)
(Sphingolipid activator protein 2) (SAP-2)
Length = 81
Score = 52.8 bits (125), Expect = 1e-06
Identities = 23/76 (30%), Positives = 44/76 (57%)
Frame = +1
Query: 211 CKACKLILHELIKEIGPDHSEETIKKALKIICKKVPKVYHKKCDVFVDKYTNDIIKYIQE 390
CKAC+ ++ ++++ I + +EE I AL +C +P+ + C VD Y + I+ + +
Sbjct: 5 CKACEYVVKKVMELIDNNRTEEKIIHALDSVCALLPESVSEVCQEVVDTYGDSIVALLLQ 64
Query: 391 NIPVDEICKKLGLCKS 438
+ + +C +LGLC S
Sbjct: 65 EMSPELVCSELGLCMS 80
Score = 52.4 bits (124), Expect = 2e-06
Identities = 23/76 (30%), Positives = 44/76 (57%)
Frame = +1
Query: 478 CKACKLILHELIKEIGPDHSEETIKKALKIICKKVPKVYHKKCDVFVDKYTNDIIKYIQK 657
CKAC+ ++ ++++ I + +EE I AL +C +P+ + C VD Y + I+ + +
Sbjct: 5 CKACEYVVKKVMELIDNNRTEEKIIHALDSVCALLPESVSEVCQEVVDTYGDSIVALLLQ 64
Query: 658 NIPVDEICKKLGLCKS 705
+ + +C +LGLC S
Sbjct: 65 EMSPELVCSELGLCMS 80
Score = 35.8 bits (81), Expect = 0.15
Identities = 15/50 (30%), Positives = 27/50 (54%)
Frame = +1
Query: 22 ALKIICKKVPKVYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLCKS 171
AL +C +P+ + C VD Y + I+ + + + + +C +LGLC S
Sbjct: 31 ALDSVCALLPESVSEVCQEVVDTYGDSIVALLLQEMSPELVCSELGLCMS 80
>sp|P50405|PSPB_MOUSE Pulmonary surfactant-associated protein B precursor (SP-B)
(Pulmonary surfactant-associated proteolipid SPL(Phe))
Length = 377
Score = 48.5 bits (114), Expect = 2e-05
Identities = 58/286 (20%), Positives = 109/286 (38%), Gaps = 51/286 (17%)
Frame = +1
Query: 1 F*RTIKKALKIICKKVP-KVYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLCKSLE 177
F I+K L+ C +P K+ +C +D Y +I Y Q I IC +GLC +
Sbjct: 87 FQEAIRKFLEQECDILPLKLLVPRCRQVLDVYLPLVIDYFQSQINPKAICNHVGLCPRGQ 146
Query: 178 VK--------------------------------------YGKEVNPLECKACKLILHEL 243
K + ++ P+ C L L
Sbjct: 147 AKPEQNPGMPDAVPNPLLDKLVLPVLPGALLARPGPHTQDFSEQQLPIPLPFCWL-CRTL 205
Query: 244 IKEIGPDHSEETIKKALKIICKKVPKVYHKKCDVFVDKYTNDIIKYIQENIPVDEICKKL 423
IK + + + A+ +C VP V C ++YT ++ + + +C +
Sbjct: 206 IKRVQAVIPKGVLAVAVSQVCHVVPLVVGGICQCLAERYTVLLLDALLGRVVPQLVCGLV 265
Query: 424 GLCKSLEVKYG---KEVNPL---------ECKACKLILHELIKEIGPDHSEETIKKALKI 567
C S E G V PL EC CK ++++ + SE+ + +A+
Sbjct: 266 LRC-STEDAMGPALPAVEPLIEEWPLQDTECHFCKSVINQ-----AWNTSEQAMPQAMHQ 319
Query: 568 ICKKVPKVYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLCKS 705
C + + +KC+ FV+++ ++ + ++ C+ LG+C++
Sbjct: 320 ACLRF-WLDRQKCEQFVEQHMPQLLALVPRSQDAHITCQALGVCEA 364
Score = 48.1 bits (113), Expect = 3e-05
Identities = 35/103 (33%), Positives = 49/103 (47%), Gaps = 1/103 (0%)
Frame = +1
Query: 412 CKKLGLCKSLEVKYGKEVNPLECKACKLILHELIKEIGPDHSEETIKKALKIICKKVP-K 588
C+ LG C EV N L C+ C+ I+H L K D +E I+K L+ C +P K
Sbjct: 48 CRALGHCLQ-EVWGHAGANDL-CQECEDIVHLLTKMTKEDAFQEAIRKFLEQECDILPLK 105
Query: 589 VYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLCKSLEVK 717
+ +C +D Y +I Y Q I IC +GLC + K
Sbjct: 106 LLVPRCRQVLDVYLPLVIDYFQSQINPKAICNHVGLCPRGQAK 148
>sp|P07988|PSPB_HUMAN Pulmonary surfactant-associated protein B precursor (SP-B) (6 kDa
protein) (Pulmonary surfactant-associated proteolipid
SPL(Phe)) (18 kDa pulmonary-surfactant protein)
Length = 381
Score = 48.1 bits (113), Expect = 3e-05
Identities = 32/99 (32%), Positives = 50/99 (50%), Gaps = 1/99 (1%)
Frame = +1
Query: 145 CKKLGLCKSLEVKYGKEVNPLECKACKLILHELIKEIGPDHSEETIKKALKIICKKVP-K 321
C+ LG C L+ +G C+ C+ I+H L K ++T++K L+ C +P K
Sbjct: 49 CRALGHC--LQEVWGHVGADDLCQECEDIVHILNKMAKEAIFQDTMRKFLEQECNVLPLK 106
Query: 322 VYHKKCDVFVDKYTNDIIKYIQENIPVDEICKKLGLCKS 438
+ +C+ +D Y +I Y Q + IC LGLCKS
Sbjct: 107 LLMPQCNQVLDDYFPLVIDYFQNQTDSNGICMHLGLCKS 145
Score = 48.1 bits (113), Expect = 3e-05
Identities = 32/99 (32%), Positives = 50/99 (50%), Gaps = 1/99 (1%)
Frame = +1
Query: 412 CKKLGLCKSLEVKYGKEVNPLECKACKLILHELIKEIGPDHSEETIKKALKIICKKVP-K 588
C+ LG C L+ +G C+ C+ I+H L K ++T++K L+ C +P K
Sbjct: 49 CRALGHC--LQEVWGHVGADDLCQECEDIVHILNKMAKEAIFQDTMRKFLEQECNVLPLK 106
Query: 589 VYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLCKS 705
+ +C+ +D Y +I Y Q + IC LGLCKS
Sbjct: 107 LLMPQCNQVLDDYFPLVIDYFQNQTDSNGICMHLGLCKS 145
Score = 46.2 bits (108), Expect = 1e-04
Identities = 59/286 (20%), Positives = 111/286 (38%), Gaps = 53/286 (18%)
Frame = +1
Query: 10 TIKKALKIICKKVP-KVYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLCKSLEVKY 186
T++K L+ C +P K+ +C+ +D Y +I Y Q + IC LGLCKS + +
Sbjct: 91 TMRKFLEQECNVLPLKLLMPQCNQVLDDYFPLVIDYFQNQTDSNGICMHLGLCKSRQPEP 150
Query: 187 GKE-------VNPLECKACKLILHELIKEIGP------------DHSEETI--------- 282
+E PL +L +L+ + P D SE+
Sbjct: 151 EQEPGMSDPLPKPLRDPLPDPLLDKLVLPVLPGALQARPGPHTQDLSEQQFPIPLPYCWL 210
Query: 283 -------------KKALKI----ICKKVPKVYHKKCDVFVDKYTNDIIKYIQENIPVDEI 411
K AL + +C+ VP V C ++Y+ ++ + + +
Sbjct: 211 CRALIKRIQAMIPKGALAVAVAQVCRVVPLVAGGICQCLAERYSVILLDTLLGRMLPQLV 270
Query: 412 CKKLGLCKSLEVKYGKE-------VNPLECKACKLILHELIKEIGPDHSEETIKKALKII 570
C+ + C S++ G EC C + + + SE+ I +A+
Sbjct: 271 CRLVLRC-SMDDSAGPRSPTGEWLPRDSECHLCMSVTTQ-----AGNSSEQAIPQAMLQA 324
Query: 571 CKKVPKVYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLCKSL 708
C + +KC FV+++T ++ + + C+ LG+C ++
Sbjct: 325 CVG-SWLDREKCKQFVEQHTPQLLTLVPRGWDAHTTCQALGVCGTM 369
>sp|P22355|PSPB_RAT Pulmonary surfactant-associated protein B precursor (SP-B)
(Pulmonary surfactant-associated proteolipid SPL(Phe))
Length = 376
Score = 47.4 bits (111), Expect = 5e-05
Identities = 58/282 (20%), Positives = 113/282 (40%), Gaps = 50/282 (17%)
Frame = +1
Query: 10 TIKKALKIICKKVP-KVYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLCKSLEVKY 186
TI+K L+ C +P K+ +C +D Y +I Y Q I IC +GLC + K
Sbjct: 89 TIRKFLEQECDILPLKLLVPRCRQVLDVYLPLVIDYFQGQIKPKAICSHVGLCPLGQTKP 148
Query: 187 GKEVNPLEC----KACKLILHEL----IKEIGPDHSEETIKKALKI-------------- 300
++ L+ KL+L L + GP H+++ ++ L I
Sbjct: 149 EQKPEMLDAIPNPLLNKLVLPALPGAFLARPGP-HTQDLSEQQLPIPLPFCWLCRTLIKR 207
Query: 301 ----------------ICKKVPKVYHKKCDVFVDKYTNDIIKYIQENIPVDEICKKLGLC 432
+C VP V C ++YT ++ + + +C + C
Sbjct: 208 VQAVIPKGVLAVAVSQVCHVVPLVVGGICQCLAERYTVLLLDALLGRVVPQLVCGLVLRC 267
Query: 433 K----------SLEVKYGK-EVNPLECKACKLILHELIKEIGPDHSEETIKKALKIICKK 579
+LE K + EC CK ++++ + SE+ + +A+ C +
Sbjct: 268 STADAIGPALPALEPLIEKWPLQDTECHFCKSVINQ-----AWNTSEQAMPQAMHQACLR 322
Query: 580 VPKVYHKKCDVFVDKYTNDIIKYIQKNIPVDEICKKLGLCKS 705
+ +KC+ FV+++ ++ + ++ C+ LG+C++
Sbjct: 323 F-WLDRQKCEQFVEQHMPQLLALVPRSQDAHTSCQALGVCEA 363
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 99,751,359
Number of Sequences: 369166
Number of extensions: 2261563
Number of successful extensions: 8191
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3831
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8106
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8245425915
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)