Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_02748
(627 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 146 5e-35
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 144 1e-34
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 143 3e-34
sp|P23561|STE11_YEAST Serine/threonine-protein kinase STE11 140 3e-33
sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinas... 139 6e-33
sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinas... 139 8e-33
sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinas... 138 1e-32
sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinas... 137 2e-32
sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinas... 135 7e-32
sp|O95382|M3K6_HUMAN Mitogen-activated protein kinase kinas... 132 9e-31
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 146 bits (368), Expect = 5e-35
Identities = 88/215 (40%), Positives = 120/215 (55%), Gaps = 7/215 (3%)
Frame = +2
Query: 2 FGNVYKINKSSSSPELAIKTI----GKAKNDDIEKEAKYLENNINKNRDNLKHDNIVKYF 169
FG VY S LAIK + A + + + LE + + NL H NIV+Y
Sbjct: 79 FGRVYMGMNLDSGELLAIKQVLIAPSSASKEKTQGHIRELEEEVQLLK-NLSHPNIVRYL 137
Query: 170 GAINKQWKIYIFMEFIDGGSISEKISLNNYQPLPVKTIKLYTKQILRGLKFLHQSNIIHR 349
G + + + I MEF+ GGSIS L + P I +YTKQ+L GL++LH + I+HR
Sbjct: 138 GTVRESDSLNILMEFVPGGSISSL--LEKFGSFPEPVIIMYTKQLLLGLEYLHNNGIMHR 195
Query: 350 DIKSANILITKQDCIKIVDFGLSR---FLQTVSRQEKKKKTFCGTANYMSPEVINDESYG 520
DIK ANIL+ + CI++ DFG S+ L TV+ + K GT +M+PEVI +
Sbjct: 196 DIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMK----GTPYWMAPEVILQTGHS 251
Query: 521 FPADIWSVGATVFEMATGRPPYHWVSNEIAAGVKI 625
F ADIWSVG TV EMATG+PP+ + AA + I
Sbjct: 252 FSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHI 286
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 144 bits (364), Expect = 1e-34
Identities = 84/215 (39%), Positives = 118/215 (54%), Gaps = 11/215 (5%)
Frame = +2
Query: 2 FGNVYKINKSSSSPELAIKTIGKAKN-----------DDIEKEAKYLENNINKNRDNLKH 148
FG VY S LA+K + A N ++E+E K L+N L H
Sbjct: 80 FGTVYMGMNLDSGELLAVKQVLIAANFASKEKTQAHIQELEEEVKLLKN--------LSH 131
Query: 149 DNIVKYFGAINKQWKIYIFMEFIDGGSISEKISLNNYQPLPVKTIKLYTKQILRGLKFLH 328
NIV+Y G + + + I +EF+ GGSIS L + P P ++ YT+Q+L GL++LH
Sbjct: 132 PNIVRYLGTVREDDTLNILLEFVPGGSISSL--LEKFGPFPESVVRTYTRQLLLGLEYLH 189
Query: 329 QSNIIHRDIKSANILITKQDCIKIVDFGLSRFLQTVSRQEKKKKTFCGTANYMSPEVIND 508
I+HRDIK ANIL+ + CIK+ DFG S+ + ++ K+ GT +M+PEVI
Sbjct: 190 NHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATM-TGAKSMKGTPYWMAPEVILQ 248
Query: 509 ESYGFPADIWSVGATVFEMATGRPPYHWVSNEIAA 613
+ F ADIWSVG TV EM TG+ P+ E+AA
Sbjct: 249 TGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAA 283
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 143 bits (361), Expect = 3e-34
Identities = 87/211 (41%), Positives = 115/211 (54%), Gaps = 7/211 (3%)
Frame = +2
Query: 2 FGNVYKINKSSSSPELAIK----TIGKAKNDDIEKEAKYLENNINKNRDNLKHDNIVKYF 169
FG VY S LA+K T A + + + LE + K NL H NIV+Y
Sbjct: 79 FGTVYMGMNLDSGELLAVKQVLITSNCASKEKTQAHIQELEEEV-KLLKNLSHPNIVRYL 137
Query: 170 GAINKQWKIYIFMEFIDGGSISEKISLNNYQPLPVKTIKLYTKQILRGLKFLHQSNIIHR 349
G + + + I +EF+ GGSIS L + P ++ YT Q+L GL++LH I+HR
Sbjct: 138 GTVREDETLNILLEFVPGGSISSL--LEKFGAFPESVVRTYTNQLLLGLEYLHNHAIMHR 195
Query: 350 DIKSANILITKQDCIKIVDFGLSR---FLQTVSRQEKKKKTFCGTANYMSPEVINDESYG 520
DIK ANIL+ Q CIK+ DFG S+ L T+S + K GT +M+PEVI +
Sbjct: 196 DIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMK----GTPYWMAPEVILQTGHS 251
Query: 521 FPADIWSVGATVFEMATGRPPYHWVSNEIAA 613
F ADIWSVG TV EM TG+ P+ EIAA
Sbjct: 252 FSADIWSVGCTVIEMVTGKAPWSQQYKEIAA 282
>sp|P23561|STE11_YEAST Serine/threonine-protein kinase STE11
Length = 738
Score = 140 bits (352), Expect = 3e-33
Identities = 70/160 (43%), Positives = 105/160 (65%)
Frame = +2
Query: 107 LENNINKNRDNLKHDNIVKYFGAINKQWKIYIFMEFIDGGSISEKISLNNYQPLPVKTIK 286
L++ +N ++ L H+NIV Y+GA + + IF+E++ GGS+S LNNY P I
Sbjct: 522 LQHEMNLLKE-LHHENIVTYYGASQEGGNLNIFLEYVPGGSVSSM--LNNYGPFEESLIT 578
Query: 287 LYTKQILRGLKFLHQSNIIHRDIKSANILITKQDCIKIVDFGLSRFLQTVSRQEKKKKTF 466
+T+QIL G+ +LH+ NIIHRDIK ANILI + C+KI DFG+S+ L +++++ K+ +
Sbjct: 579 NFTRQILIGVAYLHKKNIIHRDIKGANILIDIKGCVKITDFGISKKLSPLNKKQNKRASL 638
Query: 467 CGTANYMSPEVINDESYGFPADIWSVGATVFEMATGRPPY 586
G+ +MSPEV+ + ADIWS G V EM TG+ P+
Sbjct: 639 QGSVFWMSPEVVKQTATTAKADIWSTGCVVIEMFTGKHPF 678
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 139 bits (350), Expect = 6e-33
Identities = 82/209 (39%), Positives = 118/209 (56%), Gaps = 3/209 (1%)
Frame = +2
Query: 2 FGNVYKINKSSSSPELAIKTIG-KAKNDDIEKEAKYLENNINKNRDNLKHDNIVKYFGAI 178
FG VY + ELA K + + + KE LE I + NL+H+ IV+Y+G +
Sbjct: 373 FGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLK-NLQHERIVQYYGCL 431
Query: 179 NKQWK--IYIFMEFIDGGSISEKISLNNYQPLPVKTIKLYTKQILRGLKFLHQSNIIHRD 352
+ + + IFME++ GGS+ ++ L Y L + YT+QIL G+ +LH + I+HRD
Sbjct: 432 RDRAEKTLTIFMEYMPGGSVKDQ--LKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRD 489
Query: 353 IKSANILITKQDCIKIVDFGLSRFLQTVSRQEKKKKTFCGTANYMSPEVINDESYGFPAD 532
IK ANIL +K+ DFG S+ LQT+ ++ GT +MSPEVI+ E YG AD
Sbjct: 490 IKGANILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVTGTPYWMSPEVISGEGYGRKAD 549
Query: 533 IWSVGATVFEMATGRPPYHWVSNEIAAGV 619
+WS+G TV EM T +PP W E A +
Sbjct: 550 VWSLGCTVVEMLTEKPP--WAEYEAMAAI 576
>sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 619
Score = 139 bits (349), Expect = 8e-33
Identities = 86/209 (41%), Positives = 116/209 (55%), Gaps = 3/209 (1%)
Frame = +2
Query: 2 FGNVYKINKSSSSPELAIKTIG-KAKNDDIEKEAKYLENNINKNRDNLKHDNIVKYFGAI 178
FG VY + ELA+K + ++ + KE LE I + NL H+ IV+Y+G +
Sbjct: 367 FGRVYLCYDVDTGRELAVKQVQFNPESPETSKEVNALECEIQLLK-NLLHERIVQYYGCL 425
Query: 179 N--KQWKIYIFMEFIDGGSISEKISLNNYQPLPVKTIKLYTKQILRGLKFLHQSNIIHRD 352
++ + IFME GGSI ++ L Y L + YT+QIL G+ +LH + I+HRD
Sbjct: 426 RDPQEKTLSIFMELSPGGSIKDQ--LKAYGALTENVTRKYTRQILEGVHYLHSNMIVHRD 483
Query: 353 IKSANILITKQDCIKIVDFGLSRFLQTVSRQEKKKKTFCGTANYMSPEVINDESYGFPAD 532
IK ANIL IK+ DFG S+ LQT+ K+ GT +MSPEVI+ E YG AD
Sbjct: 484 IKGANILRDSTGNIKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEVISGEGYGRKAD 543
Query: 533 IWSVGATVFEMATGRPPYHWVSNEIAAGV 619
IWSV TV EM T +PP W E A +
Sbjct: 544 IWSVACTVVEMLTEKPP--WAEFEAMAAI 570
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 138 bits (348), Expect = 1e-32
Identities = 82/209 (39%), Positives = 118/209 (56%), Gaps = 3/209 (1%)
Frame = +2
Query: 2 FGNVYKINKSSSSPELAIKTIG-KAKNDDIEKEAKYLENNINKNRDNLKHDNIVKYFGAI 178
FG VY + ELA K + + + KE LE I + NL+H+ IV+Y+G +
Sbjct: 373 FGRVYLCYDVDTGRELASKQVQFDPDSPETSKEVSALECEIQLLK-NLQHERIVQYYGCL 431
Query: 179 NKQWK--IYIFMEFIDGGSISEKISLNNYQPLPVKTIKLYTKQILRGLKFLHQSNIIHRD 352
+ + + IFME++ GGS+ ++ L Y L + YT+QIL G+ +LH + I+HRD
Sbjct: 432 RDRAEKILTIFMEYMPGGSVKDQ--LKAYGALTESVTRKYTRQILEGMSYLHSNMIVHRD 489
Query: 353 IKSANILITKQDCIKIVDFGLSRFLQTVSRQEKKKKTFCGTANYMSPEVINDESYGFPAD 532
IK ANIL +K+ DFG S+ LQT+ ++ GT +MSPEVI+ E YG AD
Sbjct: 490 IKGANILRDSAGNVKLGDFGASKRLQTICMSGTGIRSVTGTPYWMSPEVISGEGYGRKAD 549
Query: 533 IWSVGATVFEMATGRPPYHWVSNEIAAGV 619
+WS+G TV EM T +PP W E A +
Sbjct: 550 VWSLGCTVVEMLTEKPP--WAEYEAMAAI 576
>sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinase kinase 5 (MAPK/ERK kinase
kinase 5) (MEK kinase 5) (MEKK 5) (Apoptosis
signal-regulating kinase 1) (ASK-1)
Length = 1374
Score = 137 bits (346), Expect = 2e-32
Identities = 89/216 (41%), Positives = 128/216 (59%), Gaps = 8/216 (3%)
Frame = +2
Query: 2 FGNVYKINKSSSSPELAIKTIGKAKNDDIEKEAKY---LENNINKNRDNLKHDNIVKYFG 172
+G VY S+ +AIK I E++++Y L I ++ +LKH NIV+Y G
Sbjct: 691 YGIVYAGRDLSNQVRIAIKEIP-------ERDSRYSQPLHEEIALHK-HLKHKNIVQYLG 742
Query: 173 AINKQWKIYIFMEFIDGGSISEKISLNNYQPLP--VKTIKLYTKQILRGLKFLHQSNIIH 346
+ ++ I IFME + GGS+S + + + PL +TI YTKQIL GLK+LH + I+H
Sbjct: 743 SFSENGFIKIFMEQVPGGSLSALLR-SKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVH 801
Query: 347 RDIKSANILI-TKQDCIKIVDFGLSRFLQTVSRQEKKKKTFCGTANYMSPEVIN--DESY 517
RDIK N+LI T +KI DFG S+ L ++ +TF GT YM+PE+I+ Y
Sbjct: 802 RDIKGDNVLINTYSGVLKISDFGTSKRLAGIN---PCTETFTGTLQYMAPEIIDKGPRGY 858
Query: 518 GFPADIWSVGATVFEMATGRPPYHWVSNEIAAGVKI 625
G ADIWS+G T+ EMATG+PP++ + AA K+
Sbjct: 859 GKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKV 894
>sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 618
Score = 135 bits (341), Expect = 7e-32
Identities = 83/209 (39%), Positives = 116/209 (55%), Gaps = 3/209 (1%)
Frame = +2
Query: 2 FGNVYKINKSSSSPELAIKTIG-KAKNDDIEKEAKYLENNINKNRDNLKHDNIVKYFGAI 178
FG VY + ELA+K + + + KE LE I + N H+ IV+Y+G +
Sbjct: 366 FGRVYLCYDVDTGRELAVKQVQFDPDSPETSKEVNALECEIQLLK-NFLHERIVQYYGCL 424
Query: 179 N--KQWKIYIFMEFIDGGSISEKISLNNYQPLPVKTIKLYTKQILRGLKFLHQSNIIHRD 352
++ + IFME++ GGSI ++ L Y L + YT+QIL G+ +LH + I+HRD
Sbjct: 425 RDPQEKTLSIFMEYMPGGSIKDQ--LKAYGALTENGTRKYTRQILEGVHYLHSNMILHRD 482
Query: 353 IKSANILITKQDCIKIVDFGLSRFLQTVSRQEKKKKTFCGTANYMSPEVINDESYGFPAD 532
IK ANIL +K+ DFG S+ LQT+ K+ GT +MSPEVI+ + YG AD
Sbjct: 483 IKGANILRDSTGNVKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEVISGQGYGRKAD 542
Query: 533 IWSVGATVFEMATGRPPYHWVSNEIAAGV 619
IWSV TV EM T +PP W E A +
Sbjct: 543 IWSVACTVVEMLTEKPP--WAEFEAMAAI 569
>sp|O95382|M3K6_HUMAN Mitogen-activated protein kinase kinase kinase 6
Length = 1011
Score = 132 bits (331), Expect = 9e-31
Identities = 85/209 (40%), Positives = 124/209 (59%), Gaps = 5/209 (2%)
Frame = +2
Query: 2 FGNVYKINKSSSSPELAIKTIGKAKNDDIEKEAKYLENNINKNRDNLKHDNIVKYFGAIN 181
+G VY + +AIK I + + + ++ L I +R L+H NIV+Y G+ +
Sbjct: 382 YGVVYAGRDRHTRVRIAIKEIPERDS----RFSQPLHEEIALHR-RLRHKNIVRYLGSAS 436
Query: 182 KQWKIYIFMEFIDGGSISEKISLNNYQPLP--VKTIKLYTKQILRGLKFLHQSNIIHRDI 355
+ + IFME + GGS+S + + + PL TI YT+QIL+GL +LH ++I+HRDI
Sbjct: 437 QGGYLKIFMEEVPGGSLSSLLR-SVWGPLKDNESTISFYTRQILQGLGYLHDNHIVHRDI 495
Query: 356 KSANILI-TKQDCIKIVDFGLSRFLQTVSRQEKKKKTFCGTANYMSPEVIND--ESYGFP 526
K N+LI T +KI DFG S+ L ++ +TF GT YM+PE+I+ YG
Sbjct: 496 KGDNVLINTFSGLLKISDFGTSKRLAGIT---PCTETFTGTLQYMAPEIIDQGPRGYGKA 552
Query: 527 ADIWSVGATVFEMATGRPPYHWVSNEIAA 613
ADIWS+G TV EMATGRPP+H + + AA
Sbjct: 553 ADIWSLGCTVIEMATGRPPFHELGSPQAA 581
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 70,802,180
Number of Sequences: 369166
Number of extensions: 1388147
Number of successful extensions: 8443
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5650
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5974
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 4974853140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)