Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_02747
(508 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9VYA0|NADE_DROME Putative glutamine-dependent NAD(+) sy... 190 2e-48
sp|P38795|NADE_YEAST Glutamine-dependent NAD(+) synthetase ... 168 6e-42
sp|O74940|NADE_SCHPO Putative glutamine-dependent NAD(+) sy... 163 2e-40
sp|P08164|NADE_BACSU NH(3)-dependent NAD(+) synthetase (Spo... 54 3e-07
sp|O29262|NADE_ARCFU Probable NH(3)-dependent NAD(+) synthe... 54 3e-07
sp|Q81RP3|NADE_BACAN NH(3)-dependent NAD(+) synthetase 50 2e-06
sp|P47623|NADE_MYCGE Probable NH(3)-dependent NAD(+) synthe... 50 2e-06
sp|Q81EI2|NADE_BACCR NH(3)-dependent NAD(+) synthetase 50 2e-06
sp|Q8PZP6|NADE_METMA NH(3)-dependent NAD(+) synthetase 50 2e-06
sp|Q6L0D1|NADE_PICTO NH(3)-dependent NAD(+) synthetase 50 4e-06
>sp|Q9VYA0|NADE_DROME Putative glutamine-dependent NAD(+) synthetase (NAD(+) synthase
[glutamine-hydrolyzing])
Length = 787
Score = 190 bits (482), Expect = 2e-48
Identities = 91/152 (59%), Positives = 117/152 (76%), Gaps = 1/152 (0%)
Frame = +3
Query: 3 LLSQLYLWSKNRTGGLLVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFISYC 182
+ +QL LW +NR GGLLVL + N+DE LRGYLTKYDCSSAD+NPIG I K DL+ F++Y
Sbjct: 495 IFAQLTLWVRNRPGGLLVLGSANVDESLRGYLTKYDCSSADINPIGGISKMDLRRFLTYA 554
Query: 183 IKEFELSSLESILAAPPTAELEPLKD-GQVSQLDEVDMGMSYSELSEYGKLRKIKCCGPY 359
+F L LESI+ APPTAELEPL++ G++ Q DE DMGM+Y+ELS++G+LRK CGPY
Sbjct: 555 KDKFNLPVLESIIDAPPTAELEPLQENGELQQTDEADMGMTYAELSQFGRLRKQSFCGPY 614
Query: 360 GMFIKLLEIWKEKQLTALEIADKVKHFFVCYS 455
MF L+ WK L+ E+A+KVKHFF+CY+
Sbjct: 615 SMFCHLVATWK-SDLSPKEVAEKVKHFFLCYA 645
>sp|P38795|NADE_YEAST Glutamine-dependent NAD(+) synthetase (NAD(+) synthase
[glutamine-hydrolyzing])
Length = 714
Score = 168 bits (426), Expect = 6e-42
Identities = 86/153 (56%), Positives = 107/153 (69%), Gaps = 2/153 (1%)
Frame = +3
Query: 3 LLSQLYLWSKN--RTGGLLVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFIS 176
L +QL W + +GGLLVL + N+DECLRGYLTKYDCSSAD+NPIG I K DLK FI+
Sbjct: 500 LFAQLLPWVRGIPNSGGLLVLGSANVDECLRGYLTKYDCSSADINPIGGISKTDLKRFIA 559
Query: 177 YCIKEFELSSLESILAAPPTAELEPLKDGQVSQLDEVDMGMSYSELSEYGKLRKIKCCGP 356
Y K++ + L L A PTAELEP+ V Q DE+DMGM+Y EL +G LRK++ CGP
Sbjct: 560 YASKQYNMPILNDFLNATPTAELEPMTKDYV-QSDEIDMGMTYEELGVFGYLRKVEKCGP 618
Query: 357 YGMFIKLLEIWKEKQLTALEIADKVKHFFVCYS 455
Y MF+KLL W K LT +I++KVK FF Y+
Sbjct: 619 YSMFLKLLHQWSPK-LTPRQISEKVKRFFFFYA 650
>sp|O74940|NADE_SCHPO Putative glutamine-dependent NAD(+) synthetase (NAD(+) synthase
[glutamine-hydrolyzing])
Length = 700
Score = 163 bits (413), Expect = 2e-40
Identities = 82/150 (54%), Positives = 105/150 (70%)
Frame = +3
Query: 3 LLSQLYLWSKNRTGGLLVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFISYC 182
L +QL W + +G LLVL + N+DECLRGYLTKYDCSSAD+NPIG I K DLK F+ Y
Sbjct: 497 LFAQLLPWVRGYSGSLLVLGSSNVDECLRGYLTKYDCSSADINPIGGISKTDLKSFLRYA 556
Query: 183 IKEFELSSLESILAAPPTAELEPLKDGQVSQLDEVDMGMSYSELSEYGKLRKIKCCGPYG 362
+ +L L+ L A PTAELEP + V Q DE DMGM+Y+ELS +G+LRKI CGPY
Sbjct: 557 KEALDLPILQEFLDATPTAELEPTTESYV-QSDEADMGMTYAELSVFGRLRKISKCGPYS 615
Query: 363 MFIKLLEIWKEKQLTALEIADKVKHFFVCY 452
MF +L+ W ++ L+ ++A+KVK FF Y
Sbjct: 616 MFTQLMHQWGDR-LSPSQVAEKVKRFFHYY 644
>sp|P08164|NADE_BACSU NH(3)-dependent NAD(+) synthetase (Spore outgrowth factor B)
(Sporulation protein outB) (General stress protein 38)
(GSP38)
Length = 272
Score = 53.5 bits (127), Expect = 3e-07
Identities = 32/93 (34%), Positives = 48/93 (51%)
Frame = +3
Query: 45 GLLVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFISYCIKEFELSSLESILA 224
GLLVL T + E + G+ TKY ADL P+ + K + + EL + E +
Sbjct: 152 GLLVLGTDHAAEAVTGFFTKYGDGGADLLPLTGLTKRQGRTLLK------ELGAPERLYL 205
Query: 225 APPTAELEPLKDGQVSQLDEVDMGMSYSELSEY 323
PTA+ L D + Q DE ++G+SY E+ +Y
Sbjct: 206 KEPTAD---LLDEKPQQSDETELGISYDEIDDY 235
>sp|O29262|NADE_ARCFU Probable NH(3)-dependent NAD(+) synthetase
Length = 247
Score = 53.5 bits (127), Expect = 3e-07
Identities = 35/94 (37%), Positives = 45/94 (47%)
Frame = +3
Query: 51 LVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFISYCIKEFELSSLESILAAP 230
LV TGN E + GY TKY D PIG + K ++ +Y L I+
Sbjct: 128 LVAGTGNKSELMVGYFTKYGDGGVDFLPIGDLYKTEVFQLAAY------LGVPRRIIEKK 181
Query: 231 PTAELEPLKDGQVSQLDEVDMGMSYSELSEYGKL 332
P+A L P Q DE +MG+SY+EL E KL
Sbjct: 182 PSARLWP------GQTDEEEMGISYAELDEILKL 209
>sp|Q81RP3|NADE_BACAN NH(3)-dependent NAD(+) synthetase
Length = 272
Score = 50.4 bits (119), Expect = 2e-06
Identities = 30/93 (32%), Positives = 48/93 (51%)
Frame = +3
Query: 45 GLLVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFISYCIKEFELSSLESILA 224
GLLV+ T + E + G+ TK+ ADL P+ + K + + EL + E +
Sbjct: 152 GLLVIGTDHAAEAVTGFFTKFGDGGADLLPLTGLTKRQGRALLQ------ELGADERLYL 205
Query: 225 APPTAELEPLKDGQVSQLDEVDMGMSYSELSEY 323
PTA+L K GQ DE ++G++Y +L +Y
Sbjct: 206 KMPTADLLDEKPGQA---DETELGITYDQLDDY 235
>sp|P47623|NADE_MYCGE Probable NH(3)-dependent NAD(+) synthetase
Length = 248
Score = 50.4 bits (119), Expect = 2e-06
Identities = 41/144 (28%), Positives = 62/144 (43%)
Frame = +3
Query: 6 LSQLYLWSKNRTGGLLVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFISYCI 185
L + L++ + LVL TGN E GY TK+ + D+ P+ + K D +
Sbjct: 115 LRMITLYAYAQKHNFLVLGTGNFVEYTLGYFTKWGDGACDIAPLAWLLKED----VYKLA 170
Query: 186 KEFELSSLESILAAPPTAELEPLKDGQVSQLDEVDMGMSYSELSEYGKLRKIKCCGPYGM 365
K F + E ++ PTA L Q DE +MG++Y EL +Y K I
Sbjct: 171 KHFNIP--EIVITRAPTASLFE------GQTDETEMGITYKELDQYLKGDLI-------- 214
Query: 366 FIKLLEIWKEKQLTALEIADKVKH 437
+ EKQ L++ K +H
Sbjct: 215 ------LSSEKQKIVLDLKAKAEH 232
>sp|Q81EI2|NADE_BACCR NH(3)-dependent NAD(+) synthetase
Length = 272
Score = 50.4 bits (119), Expect = 2e-06
Identities = 30/93 (32%), Positives = 48/93 (51%)
Frame = +3
Query: 45 GLLVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFISYCIKEFELSSLESILA 224
GLLV+ T + E + G+ TK+ ADL P+ + K + + EL + E +
Sbjct: 152 GLLVIGTDHAAEAVTGFFTKFGDGGADLLPLTGLTKRQGRALLQ------ELGADERLYL 205
Query: 225 APPTAELEPLKDGQVSQLDEVDMGMSYSELSEY 323
PTA+L K GQ DE ++G++Y +L +Y
Sbjct: 206 KMPTADLLDEKPGQA---DETELGITYDQLDDY 235
>sp|Q8PZP6|NADE_METMA NH(3)-dependent NAD(+) synthetase
Length = 256
Score = 50.4 bits (119), Expect = 2e-06
Identities = 35/98 (35%), Positives = 48/98 (48%), Gaps = 4/98 (4%)
Frame = +3
Query: 51 LVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFISYCIKEFELSSL----ESI 218
+V+ TGN E L GY TKY DL PIG + K + +ELSS ES+
Sbjct: 131 MVIGTGNKTEILLGYYTKYGDGGVDLEPIGGLYKTGV----------WELSSRLGIPESL 180
Query: 219 LAAPPTAELEPLKDGQVSQLDEVDMGMSYSELSEYGKL 332
+ P+A L Q DE D+G+SY ++ E K+
Sbjct: 181 ITKKPSAGL------WAGQTDEADLGISYVKVDEVLKM 212
>sp|Q6L0D1|NADE_PICTO NH(3)-dependent NAD(+) synthetase
Length = 249
Score = 49.7 bits (117), Expect = 4e-06
Identities = 37/102 (36%), Positives = 50/102 (49%)
Frame = +3
Query: 9 SQLYLWSKNRTGGLLVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFISYCIK 188
S + ++ N GL+V T N E L GY TKY + DL PI + K+D++ SY
Sbjct: 113 SVILYYNANLLNGLVV-GTTNRTEYLIGYFTKYGDGACDLEPIEHLYKSDVRELASY--- 168
Query: 189 EFELSSLESILAAPPTAELEPLKDGQVSQLDEVDMGMSYSEL 314
L ESI+ P+A L Q DE ++GM Y EL
Sbjct: 169 ---LKVPESIIRKKPSAGL------WGDQYDEDELGMGYEEL 201
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 60,471,825
Number of Sequences: 369166
Number of extensions: 1176389
Number of successful extensions: 3162
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3075
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3142
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3206272875
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)