Planaria EST Database


DrC_02747

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_02747
         (508 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9VYA0|NADE_DROME  Putative glutamine-dependent NAD(+) sy...   190   2e-48
sp|P38795|NADE_YEAST  Glutamine-dependent NAD(+) synthetase ...   168   6e-42
sp|O74940|NADE_SCHPO  Putative glutamine-dependent NAD(+) sy...   163   2e-40
sp|P08164|NADE_BACSU  NH(3)-dependent NAD(+) synthetase (Spo...    54   3e-07
sp|O29262|NADE_ARCFU  Probable NH(3)-dependent NAD(+) synthe...    54   3e-07
sp|Q81RP3|NADE_BACAN  NH(3)-dependent NAD(+) synthetase            50   2e-06
sp|P47623|NADE_MYCGE  Probable NH(3)-dependent NAD(+) synthe...    50   2e-06
sp|Q81EI2|NADE_BACCR  NH(3)-dependent NAD(+) synthetase            50   2e-06
sp|Q8PZP6|NADE_METMA  NH(3)-dependent NAD(+) synthetase            50   2e-06
sp|Q6L0D1|NADE_PICTO  NH(3)-dependent NAD(+) synthetase            50   4e-06
>sp|Q9VYA0|NADE_DROME Putative glutamine-dependent NAD(+) synthetase (NAD(+) synthase
           [glutamine-hydrolyzing])
          Length = 787

 Score =  190 bits (482), Expect = 2e-48
 Identities = 91/152 (59%), Positives = 117/152 (76%), Gaps = 1/152 (0%)
 Frame = +3

Query: 3   LLSQLYLWSKNRTGGLLVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFISYC 182
           + +QL LW +NR GGLLVL + N+DE LRGYLTKYDCSSAD+NPIG I K DL+ F++Y 
Sbjct: 495 IFAQLTLWVRNRPGGLLVLGSANVDESLRGYLTKYDCSSADINPIGGISKMDLRRFLTYA 554

Query: 183 IKEFELSSLESILAAPPTAELEPLKD-GQVSQLDEVDMGMSYSELSEYGKLRKIKCCGPY 359
             +F L  LESI+ APPTAELEPL++ G++ Q DE DMGM+Y+ELS++G+LRK   CGPY
Sbjct: 555 KDKFNLPVLESIIDAPPTAELEPLQENGELQQTDEADMGMTYAELSQFGRLRKQSFCGPY 614

Query: 360 GMFIKLLEIWKEKQLTALEIADKVKHFFVCYS 455
            MF  L+  WK   L+  E+A+KVKHFF+CY+
Sbjct: 615 SMFCHLVATWK-SDLSPKEVAEKVKHFFLCYA 645
>sp|P38795|NADE_YEAST Glutamine-dependent NAD(+) synthetase (NAD(+) synthase
           [glutamine-hydrolyzing])
          Length = 714

 Score =  168 bits (426), Expect = 6e-42
 Identities = 86/153 (56%), Positives = 107/153 (69%), Gaps = 2/153 (1%)
 Frame = +3

Query: 3   LLSQLYLWSKN--RTGGLLVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFIS 176
           L +QL  W +    +GGLLVL + N+DECLRGYLTKYDCSSAD+NPIG I K DLK FI+
Sbjct: 500 LFAQLLPWVRGIPNSGGLLVLGSANVDECLRGYLTKYDCSSADINPIGGISKTDLKRFIA 559

Query: 177 YCIKEFELSSLESILAAPPTAELEPLKDGQVSQLDEVDMGMSYSELSEYGKLRKIKCCGP 356
           Y  K++ +  L   L A PTAELEP+    V Q DE+DMGM+Y EL  +G LRK++ CGP
Sbjct: 560 YASKQYNMPILNDFLNATPTAELEPMTKDYV-QSDEIDMGMTYEELGVFGYLRKVEKCGP 618

Query: 357 YGMFIKLLEIWKEKQLTALEIADKVKHFFVCYS 455
           Y MF+KLL  W  K LT  +I++KVK FF  Y+
Sbjct: 619 YSMFLKLLHQWSPK-LTPRQISEKVKRFFFFYA 650
>sp|O74940|NADE_SCHPO Putative glutamine-dependent NAD(+) synthetase (NAD(+) synthase
           [glutamine-hydrolyzing])
          Length = 700

 Score =  163 bits (413), Expect = 2e-40
 Identities = 82/150 (54%), Positives = 105/150 (70%)
 Frame = +3

Query: 3   LLSQLYLWSKNRTGGLLVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFISYC 182
           L +QL  W +  +G LLVL + N+DECLRGYLTKYDCSSAD+NPIG I K DLK F+ Y 
Sbjct: 497 LFAQLLPWVRGYSGSLLVLGSSNVDECLRGYLTKYDCSSADINPIGGISKTDLKSFLRYA 556

Query: 183 IKEFELSSLESILAAPPTAELEPLKDGQVSQLDEVDMGMSYSELSEYGKLRKIKCCGPYG 362
            +  +L  L+  L A PTAELEP  +  V Q DE DMGM+Y+ELS +G+LRKI  CGPY 
Sbjct: 557 KEALDLPILQEFLDATPTAELEPTTESYV-QSDEADMGMTYAELSVFGRLRKISKCGPYS 615

Query: 363 MFIKLLEIWKEKQLTALEIADKVKHFFVCY 452
           MF +L+  W ++ L+  ++A+KVK FF  Y
Sbjct: 616 MFTQLMHQWGDR-LSPSQVAEKVKRFFHYY 644
>sp|P08164|NADE_BACSU NH(3)-dependent NAD(+) synthetase (Spore outgrowth factor B)
           (Sporulation protein outB) (General stress protein 38)
           (GSP38)
          Length = 272

 Score = 53.5 bits (127), Expect = 3e-07
 Identities = 32/93 (34%), Positives = 48/93 (51%)
 Frame = +3

Query: 45  GLLVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFISYCIKEFELSSLESILA 224
           GLLVL T +  E + G+ TKY    ADL P+  + K   +  +       EL + E +  
Sbjct: 152 GLLVLGTDHAAEAVTGFFTKYGDGGADLLPLTGLTKRQGRTLLK------ELGAPERLYL 205

Query: 225 APPTAELEPLKDGQVSQLDEVDMGMSYSELSEY 323
             PTA+   L D +  Q DE ++G+SY E+ +Y
Sbjct: 206 KEPTAD---LLDEKPQQSDETELGISYDEIDDY 235
>sp|O29262|NADE_ARCFU Probable NH(3)-dependent NAD(+) synthetase
          Length = 247

 Score = 53.5 bits (127), Expect = 3e-07
 Identities = 35/94 (37%), Positives = 45/94 (47%)
 Frame = +3

Query: 51  LVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFISYCIKEFELSSLESILAAP 230
           LV  TGN  E + GY TKY     D  PIG + K ++    +Y      L     I+   
Sbjct: 128 LVAGTGNKSELMVGYFTKYGDGGVDFLPIGDLYKTEVFQLAAY------LGVPRRIIEKK 181

Query: 231 PTAELEPLKDGQVSQLDEVDMGMSYSELSEYGKL 332
           P+A L P       Q DE +MG+SY+EL E  KL
Sbjct: 182 PSARLWP------GQTDEEEMGISYAELDEILKL 209
>sp|Q81RP3|NADE_BACAN NH(3)-dependent NAD(+) synthetase
          Length = 272

 Score = 50.4 bits (119), Expect = 2e-06
 Identities = 30/93 (32%), Positives = 48/93 (51%)
 Frame = +3

Query: 45  GLLVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFISYCIKEFELSSLESILA 224
           GLLV+ T +  E + G+ TK+    ADL P+  + K   +  +       EL + E +  
Sbjct: 152 GLLVIGTDHAAEAVTGFFTKFGDGGADLLPLTGLTKRQGRALLQ------ELGADERLYL 205

Query: 225 APPTAELEPLKDGQVSQLDEVDMGMSYSELSEY 323
             PTA+L   K GQ    DE ++G++Y +L +Y
Sbjct: 206 KMPTADLLDEKPGQA---DETELGITYDQLDDY 235
>sp|P47623|NADE_MYCGE Probable NH(3)-dependent NAD(+) synthetase
          Length = 248

 Score = 50.4 bits (119), Expect = 2e-06
 Identities = 41/144 (28%), Positives = 62/144 (43%)
 Frame = +3

Query: 6   LSQLYLWSKNRTGGLLVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFISYCI 185
           L  + L++  +    LVL TGN  E   GY TK+   + D+ P+  + K D    +    
Sbjct: 115 LRMITLYAYAQKHNFLVLGTGNFVEYTLGYFTKWGDGACDIAPLAWLLKED----VYKLA 170

Query: 186 KEFELSSLESILAAPPTAELEPLKDGQVSQLDEVDMGMSYSELSEYGKLRKIKCCGPYGM 365
           K F +   E ++   PTA L         Q DE +MG++Y EL +Y K   I        
Sbjct: 171 KHFNIP--EIVITRAPTASLFE------GQTDETEMGITYKELDQYLKGDLI-------- 214

Query: 366 FIKLLEIWKEKQLTALEIADKVKH 437
                 +  EKQ   L++  K +H
Sbjct: 215 ------LSSEKQKIVLDLKAKAEH 232
>sp|Q81EI2|NADE_BACCR NH(3)-dependent NAD(+) synthetase
          Length = 272

 Score = 50.4 bits (119), Expect = 2e-06
 Identities = 30/93 (32%), Positives = 48/93 (51%)
 Frame = +3

Query: 45  GLLVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFISYCIKEFELSSLESILA 224
           GLLV+ T +  E + G+ TK+    ADL P+  + K   +  +       EL + E +  
Sbjct: 152 GLLVIGTDHAAEAVTGFFTKFGDGGADLLPLTGLTKRQGRALLQ------ELGADERLYL 205

Query: 225 APPTAELEPLKDGQVSQLDEVDMGMSYSELSEY 323
             PTA+L   K GQ    DE ++G++Y +L +Y
Sbjct: 206 KMPTADLLDEKPGQA---DETELGITYDQLDDY 235
>sp|Q8PZP6|NADE_METMA NH(3)-dependent NAD(+) synthetase
          Length = 256

 Score = 50.4 bits (119), Expect = 2e-06
 Identities = 35/98 (35%), Positives = 48/98 (48%), Gaps = 4/98 (4%)
 Frame = +3

Query: 51  LVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFISYCIKEFELSSL----ESI 218
           +V+ TGN  E L GY TKY     DL PIG + K  +          +ELSS     ES+
Sbjct: 131 MVIGTGNKTEILLGYYTKYGDGGVDLEPIGGLYKTGV----------WELSSRLGIPESL 180

Query: 219 LAAPPTAELEPLKDGQVSQLDEVDMGMSYSELSEYGKL 332
           +   P+A L         Q DE D+G+SY ++ E  K+
Sbjct: 181 ITKKPSAGL------WAGQTDEADLGISYVKVDEVLKM 212
>sp|Q6L0D1|NADE_PICTO NH(3)-dependent NAD(+) synthetase
          Length = 249

 Score = 49.7 bits (117), Expect = 4e-06
 Identities = 37/102 (36%), Positives = 50/102 (49%)
 Frame = +3

Query: 9   SQLYLWSKNRTGGLLVLATGNLDECLRGYLTKYDCSSADLNPIGSICKNDLKMFISYCIK 188
           S +  ++ N   GL+V  T N  E L GY TKY   + DL PI  + K+D++   SY   
Sbjct: 113 SVILYYNANLLNGLVV-GTTNRTEYLIGYFTKYGDGACDLEPIEHLYKSDVRELASY--- 168

Query: 189 EFELSSLESILAAPPTAELEPLKDGQVSQLDEVDMGMSYSEL 314
              L   ESI+   P+A L         Q DE ++GM Y EL
Sbjct: 169 ---LKVPESIIRKKPSAGL------WGDQYDEDELGMGYEEL 201
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 60,471,825
Number of Sequences: 369166
Number of extensions: 1176389
Number of successful extensions: 3162
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3075
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3142
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3206272875
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)