Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_02738
(878 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P28740|KIF2_MOUSE Kinesin-like protein KIF2 258 2e-68
sp|O00139|KIF2_HUMAN Kinesin-like protein KIF2 (Kinesin-2) ... 249 7e-66
sp|Q960Z0|KI10A_DROME Kinesin-like protein Klp10A (Kinesin-... 247 3e-65
sp|Q922S8|KIF2C_MOUSE Kinesin-like protein KIF2C (Mitotic c... 227 4e-59
sp|P70096|KIF2C_CRIGR Kinesin-like protein KIF2C (Mitotic c... 224 2e-58
sp|Q99661|KIF2C_HUMAN Kinesin-like protein KIF2C (Mitotic c... 223 7e-58
sp|Q91637|KIF2_XENLA Kinesin-like protein KIF2 (Kinesin-rel... 223 7e-58
sp|Q95LP1|KIF2C_MACFA Kinesin-like protein KIF2C (Mitotic c... 222 1e-57
sp|Q91636|KCM1_XENLA Kinesin central motor 1 (XKCM1) 213 4e-55
sp|Q62909|KIF2C_RAT Kinesin-like protein KIF2C (Mitotic cen... 211 2e-54
>sp|P28740|KIF2_MOUSE Kinesin-like protein KIF2
Length = 716
Score = 258 bits (658), Expect = 2e-68
Identities = 144/295 (48%), Positives = 192/295 (65%), Gaps = 3/295 (1%)
Frame = +1
Query: 1 NLGRTSGQTSANAHSSRSHAVFQIILKKRTTNRLWGKFSLIDLAGNERGADTSSSDRNTR 180
N RTSGQTSANAHSSRSHAVFQIIL+++ +L GKFSLIDLAGNERGADTSS+DR TR
Sbjct: 390 NSCRTSGQTSANAHSSRSHAVFQIILRRK--GKLHGKFSLIDLAGNERGADTSSADRQTR 447
Query: 181 MEGAEINKSLLALKECIRALGRKGAHRPFRASKLTQVLRDSFIGTKSRTCMISMISPGLL 360
+EGAEINKSLL LKECIRALGR H PFRASKLTQVLRDSFIG SRTCMI+ ISPG+
Sbjct: 448 LEGAEINKSLLRLKECIRALGRNKPHTPFRASKLTQVLRDSFIGENSRTCMIATISPGMA 507
Query: 361 SCEHTLNTLRYADRVKELGPGGNSDDNLIDNNAQHYISGSGDYEN---NYNKMSAXXXXX 531
SCE+TLNTLRYA+RVKE G + ++ +S S DY++ + ++
Sbjct: 508 SCENTLNTLRYANRVKEFGISPSDIPFSQGGGSRPDLSPSYDYDDFSPSITRVKELTVNP 567
Query: 532 XXXXXXHPGKTPISNISEYPDESYLDEEYDDNLDMENGDEDDDLAILRSTNADFLDDEMY 711
HP I +P ++ + + + + DDL +L N + + +++
Sbjct: 568 AAAGDVHP-------IMHHPPSQI--DDLETQWGVGSSPQRDDLKLLCEQNEEEVSPQLF 618
Query: 712 NFQELVNKIEEMEDAVCEKHKNIVEKFPSYLKRHSVLFKMTKQVDYPIDDYAVKL 876
F E V+++ EME+ V E H+ + ++ +++ L +MT++VDY +D YA +L
Sbjct: 619 TFHEAVSQMVEMEEQVVEDHRAVFQESIRWIEDEKALLEMTEEVDYDVDSYATQL 673
>sp|O00139|KIF2_HUMAN Kinesin-like protein KIF2 (Kinesin-2) (HK2)
Length = 679
Score = 249 bits (636), Expect = 7e-66
Identities = 144/292 (49%), Positives = 185/292 (63%)
Frame = +1
Query: 1 NLGRTSGQTSANAHSSRSHAVFQIILKKRTTNRLWGKFSLIDLAGNERGADTSSSDRNTR 180
N RTSGQTSANAHSSRSHAVFQIIL+++ +L GKFSLIDLAGNERGADTSS+DR TR
Sbjct: 391 NSCRTSGQTSANAHSSRSHAVFQIILRRK--GKLHGKFSLIDLAGNERGADTSSADRQTR 448
Query: 181 MEGAEINKSLLALKECIRALGRKGAHRPFRASKLTQVLRDSFIGTKSRTCMISMISPGLL 360
+EGAEINKSLLALKECIRALGR H PFRASKLTQVLRDSFIG SRTCMI+ ISPG+
Sbjct: 449 LEGAEINKSLLALKECIRALGRNKPHTPFRASKLTQVLRDSFIGENSRTCMIATISPGMA 508
Query: 361 SCEHTLNTLRYADRVKELGPGGNSDDNLIDNNAQHYISGSGDYENNYNKMSAXXXXXXXX 540
SCE+TLNTLRYA+RVKEL +D A +GD
Sbjct: 509 SCENTLNTLRYANRVKEL---------TVDPTA------AGD------------------ 535
Query: 541 XXXHPGKTPISNISEYPDESYLDEEYDDNLDMENGDEDDDLAILRSTNADFLDDEMYNFQ 720
+ I +P ++ + + + + DDL +L N + + +++ F
Sbjct: 536 ---------VRPIMHHPPNQI--DDLETQWGVGSSPQRDDLKLLCEQNEEEVSPQLFTFH 584
Query: 721 ELVNKIEEMEDAVCEKHKNIVEKFPSYLKRHSVLFKMTKQVDYPIDDYAVKL 876
E V+++ EME+ V E H+ + ++ +L+ L +MT++VDY +D YA +L
Sbjct: 585 EAVSQMVEMEEQVVEDHRAVFQESIRWLEDEKALLEMTEEVDYDVDSYATQL 636
>sp|Q960Z0|KI10A_DROME Kinesin-like protein Klp10A (Kinesin-like protein at cytological
position 10A)
Length = 805
Score = 247 bits (631), Expect = 3e-65
Identities = 142/297 (47%), Positives = 184/297 (61%), Gaps = 9/297 (3%)
Frame = +1
Query: 1 NLGRTSGQTSANAHSSRSHAVFQIILKKRTTNRLWGKFSLIDLAGNERGADTSSSDRNTR 180
N RTSGQTSAN++SSRSHAVFQI+L+ + + ++ GKFS IDLAGNERG DTSS+DR TR
Sbjct: 473 NAARTSGQTSANSNSSRSHAVFQIVLRPQGSTKIHGKFSFIDLAGNERGVDTSSADRQTR 532
Query: 181 MEGAEINKSLLALKECIRALGRKGAHRPFRASKLTQVLRDSFIGTKSRTCMISMISPGLL 360
MEGAEINKSLLALKECIRALG++ AH PFR SKLTQVLRDSFIG KS+TCMI+MISPGL
Sbjct: 533 MEGAEINKSLLALKECIRALGKQSAHLPFRVSKLTQVLRDSFIGEKSKTCMIAMISPGLS 592
Query: 361 SCEHTLNTLRYADRVKEL--------GPGGNSDDNLIDNNAQHYISGSGDYENNYNKMSA 516
SCEHTLNTLRYADRVKEL PGG+++ I + D E M
Sbjct: 593 SCEHTLNTLRYADRVKELVVKDIVEVCPGGDTEP----------IEITDDEEEEELNMVH 642
Query: 517 XXXXXXXXXXXHPGKTPISNISEYPDESYLDEEYDDNLDMENGDEDD-DLAILRSTNADF 693
P + + S ++N + +NG+ + DLA+L S +
Sbjct: 643 PHSHQLHPNSHAPASQSNNQRAPASHHSGAVIHNNNNNNNKNGNAGNMDLAMLSSLSEHE 702
Query: 694 LDDEMYNFQELVNKIEEMEDAVCEKHKNIVEKFPSYLKRHSVLFKMTKQVDYPIDDY 864
+ DE+ + ++ +++ E+ V E H+ + ++L L+ +T VDY D Y
Sbjct: 703 MSDELIVQHQAIDDLQQTEEMVVEYHRTVNATLETFLAESKALYNLTNYVDYDQDSY 759
>sp|Q922S8|KIF2C_MOUSE Kinesin-like protein KIF2C (Mitotic centromere-associated kinesin)
(MCAK)
Length = 721
Score = 227 bits (578), Expect = 4e-59
Identities = 137/288 (47%), Positives = 173/288 (60%)
Frame = +1
Query: 10 RTSGQTSANAHSSRSHAVFQIILKKRTTNRLWGKFSLIDLAGNERGADTSSSDRNTRMEG 189
RTSGQT AN++SSRSHA FQI+L RT RL GKFSL+DLAGNERGADTSS+DR TRMEG
Sbjct: 452 RTSGQTFANSNSSRSHACFQILL--RTKGRLHGKFSLVDLAGNERGADTSSADRQTRMEG 509
Query: 190 AEINKSLLALKECIRALGRKGAHRPFRASKLTQVLRDSFIGTKSRTCMISMISPGLLSCE 369
AEINKSLLALKECIRALG+ AH PFR SKLTQVLRDSFIG SRTCMI+MISPG+ SCE
Sbjct: 510 AEINKSLLALKECIRALGQNKAHTPFRESKLTQVLRDSFIGENSRTCMIAMISPGISSCE 569
Query: 370 HTLNTLRYADRVKELGPGGNSDDNLIDNNAQHYISGSGDYENNYNKMSAXXXXXXXXXXX 549
+TLNTLRYADRVKEL P
Sbjct: 570 YTLNTLRYADRVKELSPHSGPS-------------------------------------- 591
Query: 550 HPGKTPISNISEYPDESYLDEEYDDNLDMENGDEDDDLAILRSTNADFLDDEMYNFQELV 729
G+ P+ +E + S N G+E+++L+ +M +F E +
Sbjct: 592 --GEQPVQMETEVMEAS-------SNGTSLTGNEEEELS-----------SQMSSFNEAM 631
Query: 730 NKIEEMEDAVCEKHKNIVEKFPSYLKRHSVLFKMTKQVDYPIDDYAVK 873
+I E+E+ E+ + I+++ P++L+ L +MT Q DY ++ + K
Sbjct: 632 TQIRELEERALEELREIIQQGPNWLE----LSEMTDQPDYDLETFVNK 675
>sp|P70096|KIF2C_CRIGR Kinesin-like protein KIF2C (Mitotic centromere-associated kinesin)
(MCAK) (Kinesin-like protein 6)
Length = 718
Score = 224 bits (571), Expect = 2e-58
Identities = 137/288 (47%), Positives = 169/288 (58%)
Frame = +1
Query: 10 RTSGQTSANAHSSRSHAVFQIILKKRTTNRLWGKFSLIDLAGNERGADTSSSDRNTRMEG 189
RTSGQT AN++SSRSHA FQI+L R RL GKFSL+DLAGNERGADTSS+DR TRMEG
Sbjct: 450 RTSGQTFANSNSSRSHACFQILL--RAKGRLHGKFSLVDLAGNERGADTSSADRQTRMEG 507
Query: 190 AEINKSLLALKECIRALGRKGAHRPFRASKLTQVLRDSFIGTKSRTCMISMISPGLLSCE 369
AEINKSLLALKECIRALG+ AH PFR SKLTQVLRDSFIG SRTCMI+MISPG+ SCE
Sbjct: 508 AEINKSLLALKECIRALGQNKAHTPFRESKLTQVLRDSFIGENSRTCMIAMISPGISSCE 567
Query: 370 HTLNTLRYADRVKELGPGGNSDDNLIDNNAQHYISGSGDYENNYNKMSAXXXXXXXXXXX 549
+TLNTLRYADRVKEL P
Sbjct: 568 YTLNTLRYADRVKELSPHSGLS-------------------------------------- 589
Query: 550 HPGKTPISNISEYPDESYLDEEYDDNLDMENGDEDDDLAILRSTNADFLDDEMYNFQELV 729
G+ PI +E +ME LA+ + L +M +F E +
Sbjct: 590 --GEQPIQMETE---------------EMEASSNGTSLAV-NFKEEEELSSQMSSFNEAM 631
Query: 730 NKIEEMEDAVCEKHKNIVEKFPSYLKRHSVLFKMTKQVDYPIDDYAVK 873
++I E+E+ E+ + I+++ P +L+ L +MT Q DY ++ + K
Sbjct: 632 SQIRELEERAMEELREIIQQGPGWLE----LSEMTDQPDYDLETFVNK 675
>sp|Q99661|KIF2C_HUMAN Kinesin-like protein KIF2C (Mitotic centromere-associated kinesin)
(MCAK) (Kinesin-like protein 6)
Length = 725
Score = 223 bits (567), Expect = 7e-58
Identities = 138/289 (47%), Positives = 173/289 (59%), Gaps = 1/289 (0%)
Frame = +1
Query: 10 RTSGQTSANAHSSRSHAVFQIILKKRTTNRLWGKFSLIDLAGNERGADTSSSDRNTRMEG 189
RTSGQT AN++SSRSHA FQIIL R R+ GKFSL+DLAGNERGADTSS+DR TRMEG
Sbjct: 456 RTSGQTFANSNSSRSHACFQIIL--RAKGRMHGKFSLVDLAGNERGADTSSADRQTRMEG 513
Query: 190 AEINKSLLALKECIRALGRKGAHRPFRASKLTQVLRDSFIGTKSRTCMISMISPGLLSCE 369
AEINKSLLALKECIRALG+ AH PFR SKLTQVLRDSFIG SRTCMI+ ISPG+ SCE
Sbjct: 514 AEINKSLLALKECIRALGQNKAHTPFRESKLTQVLRDSFIGENSRTCMIATISPGISSCE 573
Query: 370 HTLNTLRYADRVKELGP-GGNSDDNLIDNNAQHYISGSGDYENNYNKMSAXXXXXXXXXX 546
+TLNTLRYADRVKEL P G S + LI + + S
Sbjct: 574 YTLNTLRYADRVKELSPHSGPSGEQLIQMETEEMEACSN--------------------- 612
Query: 547 XHPGKTPISNISEYPDESYLDEEYDDNLDMENGDEDDDLAILRSTNADFLDDEMYNFQEL 726
G N+S+ E+++L+ +M +F E
Sbjct: 613 ---GALIPGNLSK---------------------EEEELS-----------SQMSSFNEA 637
Query: 727 VNKIEEMEDAVCEKHKNIVEKFPSYLKRHSVLFKMTKQVDYPIDDYAVK 873
+ +I E+E+ E+ K I+++ P +L+ L +MT+Q DY ++ + K
Sbjct: 638 MTQIRELEEKAMEELKEIIQQGPDWLE----LSEMTEQPDYDLETFVNK 682
>sp|Q91637|KIF2_XENLA Kinesin-like protein KIF2 (Kinesin-related protein XKIF2)
Length = 682
Score = 223 bits (567), Expect = 7e-58
Identities = 138/297 (46%), Positives = 173/297 (58%), Gaps = 5/297 (1%)
Frame = +1
Query: 1 NLGRTSGQTSANAHSSRSHAVFQIILKKRTTNRLWGKFSLIDLAGNERGADTSSSDRNTR 180
N RTSGQTSANAHSSRSHAVFQIIL+K+ ++ GKFSLIDLAGNERGADTSS+DR TR
Sbjct: 393 NSCRTSGQTSANAHSSRSHAVFQIILRKK--GKMHGKFSLIDLAGNERGADTSSADRQTR 450
Query: 181 MEGAEINKSLLALKECIRALGRKGAHRPFRASKLTQVLRDSFIGTKSRTCMISMISPGLL 360
+EGAEINKSLLALKECIRALGR H PFRASKLTQVLRDSFIG SRTCMI+ ISPG+
Sbjct: 451 LEGAEINKSLLALKECIRALGRNKPHTPFRASKLTQVLRDSFIGENSRTCMIATISPGMA 510
Query: 361 SCEHTLNTLRYADRVKELGPGGNSDDNLIDNNAQHYISGSGDYENNYNKMSAXXXXXXXX 540
SCE+TLNTLR Y N ++
Sbjct: 511 SCENTLNTLR--------------------------------YANRVKELDPSRC----- 533
Query: 541 XXXHPGKTPISNISEYPDESYLDEEYDDNLDMENGDEDDDLAILRSTNADFLDDEMYNFQ 720
+ P + + P S++ + + + DDL +L N + + +++ F
Sbjct: 534 ------RRPPPHDTSCPQTSWMTWKQCGEWGV--APQRDDLKLLCEQNEEEVSPQLFTFH 585
Query: 721 ELVNKIEEMEDAVCEKHKNIVEKFPSYLKR-----HSVLFKMTKQVDYPIDDYAVKL 876
E V+++ EME+ V E H+ + FP R L +MT++VDY D YA +L
Sbjct: 586 EAVSQMVEMEEQVVEDHRAV---FPGTSIRWLEGLKKCLLEMTEEVDYDADSYATQL 639
>sp|Q95LP1|KIF2C_MACFA Kinesin-like protein KIF2C (Mitotic centromere-associated kinesin)
(MCAK)
Length = 671
Score = 222 bits (565), Expect = 1e-57
Identities = 137/289 (47%), Positives = 173/289 (59%), Gaps = 1/289 (0%)
Frame = +1
Query: 10 RTSGQTSANAHSSRSHAVFQIILKKRTTNRLWGKFSLIDLAGNERGADTSSSDRNTRMEG 189
RTSGQT AN++SSRSHA FQI+L R R+ GKFSL+DLAGNERGADTSS+DR TRMEG
Sbjct: 402 RTSGQTFANSNSSRSHACFQILL--RAKGRMHGKFSLVDLAGNERGADTSSADRQTRMEG 459
Query: 190 AEINKSLLALKECIRALGRKGAHRPFRASKLTQVLRDSFIGTKSRTCMISMISPGLLSCE 369
AEINKSLLALKECIRALG+ AH PFR SKLTQVLRDSFIG SRTCMI+ ISPG+ SCE
Sbjct: 460 AEINKSLLALKECIRALGQNKAHTPFRESKLTQVLRDSFIGENSRTCMIATISPGISSCE 519
Query: 370 HTLNTLRYADRVKELGP-GGNSDDNLIDNNAQHYISGSGDYENNYNKMSAXXXXXXXXXX 546
+TLNTLRYADRVKEL P G S + LI + + S
Sbjct: 520 YTLNTLRYADRVKELSPHSGPSGEQLIQMETEEMEACSN--------------------- 558
Query: 547 XHPGKTPISNISEYPDESYLDEEYDDNLDMENGDEDDDLAILRSTNADFLDDEMYNFQEL 726
G N+S+ E+++L+ +M +F E
Sbjct: 559 ---GALIPGNLSK---------------------EEEELS-----------SQMSSFNEA 583
Query: 727 VNKIEEMEDAVCEKHKNIVEKFPSYLKRHSVLFKMTKQVDYPIDDYAVK 873
+ +I E+E+ E+ K I+++ P +L+ L +MT+Q DY ++ + K
Sbjct: 584 MTQIRELEERAVEELKEIIQQGPDWLE----LSEMTEQPDYDLETFVNK 628
>sp|Q91636|KCM1_XENLA Kinesin central motor 1 (XKCM1)
Length = 730
Score = 213 bits (543), Expect = 4e-55
Identities = 132/288 (45%), Positives = 170/288 (59%)
Frame = +1
Query: 10 RTSGQTSANAHSSRSHAVFQIILKKRTTNRLWGKFSLIDLAGNERGADTSSSDRNTRMEG 189
RTSGQT AN SSRSHA QIIL++ + +L GKFSL+DLAGNERG DT+S+DR TRMEG
Sbjct: 460 RTSGQTFANTSSSRSHACLQIILRRGS--KLHGKFSLVDLAGNERGVDTASADRITRMEG 517
Query: 190 AEINKSLLALKECIRALGRKGAHRPFRASKLTQVLRDSFIGTKSRTCMISMISPGLLSCE 369
AEIN+SLLALKECIRALG+ +H PFR SKLTQ+LRDSFIG SRTCMI+M+SPG SCE
Sbjct: 518 AEINRSLLALKECIRALGQNKSHTPFRESKLTQILRDSFIGENSRTCMIAMLSPGFNSCE 577
Query: 370 HTLNTLRYADRVKELGPGGNSDDNLIDNNAQHYISGSGDYENNYNKMSAXXXXXXXXXXX 549
+TLNTLRYADRVKEL P N++ N D+N Q SG
Sbjct: 578 YTLNTLRYADRVKELSP-QNAETN--DDNLQMEDSGG----------------------- 611
Query: 550 HPGKTPISNISEYPDESYLDEEYDDNLDMENGDEDDDLAILRSTNADFLDDEMYNFQELV 729
I + D DEE L N +FQ+ +
Sbjct: 612 --SHASIEGLQLQDDFLLKDEE----LSTHN-----------------------SFQDAL 642
Query: 730 NKIEEMEDAVCEKHKNIVEKFPSYLKRHSVLFKMTKQVDYPIDDYAVK 873
N++ E+ED ++ + +V+K P + L +MT+Q DY ++++ ++
Sbjct: 643 NRVGELEDKAVDELRELVQKEPEWTN----LLQMTEQPDYDLENFVMQ 686
>sp|Q62909|KIF2C_RAT Kinesin-like protein KIF2C (Mitotic centromere-associated kinesin)
(MCAK) (Kinesin-related protein 2)
Length = 671
Score = 211 bits (537), Expect = 2e-54
Identities = 110/137 (80%), Positives = 119/137 (86%)
Frame = +1
Query: 10 RTSGQTSANAHSSRSHAVFQIILKKRTTNRLWGKFSLIDLAGNERGADTSSSDRNTRMEG 189
RTSGQT AN++SSRSHA FQI+L R RL GKFSL+DLAGNERGADTSS+DR TRMEG
Sbjct: 402 RTSGQTFANSNSSRSHACFQILL--RAKGRLHGKFSLVDLAGNERGADTSSADRQTRMEG 459
Query: 190 AEINKSLLALKECIRALGRKGAHRPFRASKLTQVLRDSFIGTKSRTCMISMISPGLLSCE 369
AEINKSLLALKE IRALG+ AH PFR SKLTQVLRDSFIG SRTCMI+MISPG+ SCE
Sbjct: 460 AEINKSLLALKESIRALGQNKAHTPFRESKLTQVLRDSFIGENSRTCMIAMISPGISSCE 519
Query: 370 HTLNTLRYADRVKELGP 420
+TLNTLRYADRVKEL P
Sbjct: 520 YTLNTLRYADRVKELSP 536
Score = 41.6 bits (96), Expect = 0.003
Identities = 23/82 (28%), Positives = 43/82 (52%)
Frame = +1
Query: 628 LDMENGDEDDDLAILRSTNADFLDDEMYNFQELVNKIEEMEDAVCEKHKNIVEKFPSYLK 807
++ E D A L + L +M +F E + +I E+E+ E+ + I+++ PS+L+
Sbjct: 548 METEEMDASSHGASLTGNEEEELSSQMSSFNEAMTQIRELEERAMEELREIIQQGPSWLE 607
Query: 808 RHSVLFKMTKQVDYPIDDYAVK 873
L +MT Q DY ++ + K
Sbjct: 608 ----LSEMTDQPDYDLETFVNK 625
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.314 0.131 0.375
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 90,666,812
Number of Sequences: 369166
Number of extensions: 1732231
Number of successful extensions: 5467
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4378
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4953
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8742211660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)