Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_02690
(624 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 118 1e-26
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 113 4e-25
sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinas... 112 6e-25
sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinas... 112 6e-25
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 112 1e-24
sp|O95382|M3K6_HUMAN Mitogen-activated protein kinase kinas... 110 4e-24
sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinas... 109 5e-24
sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinas... 108 8e-24
sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinas... 107 2e-23
sp|Q12263|GIN4_YEAST Serine/threonine-protein kinase GIN4 104 2e-22
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 118 bits (295), Expect = 1e-26
Identities = 69/183 (37%), Positives = 100/183 (54%), Gaps = 3/183 (1%)
Frame = +3
Query: 33 LNHENIVKYFGMIETATKWKILMEWMEKGSLGDEIRSTGSLPDDKIARYCKDILQAVAFL 212
L+H NIV+Y G + + ILME++ GS+ + GS P+ I Y K +L + +L
Sbjct: 128 LSHPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQLLLGLEYL 187
Query: 213 HHNKIIHRDIKPRNILIDNNHRCKLSICDISRL---LPNDECASTFWGTPNYWAPEIFIG 383
H+N I+HRDIK NIL+DN +L+ S+ L A + GTP + APE+ +
Sbjct: 188 HNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPYWMAPEVILQ 247
Query: 384 EPYNYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGTTRKLNYTPPRQISNEMQ 563
+++ ADIWSVG TVI M TG PP+ E Y A + IG T K + P +S E +
Sbjct: 248 TGHSFSADIWSVGCTVIEMATGKPPWSE--QYQQFAAVLHIGRT-KAHPPIPEDLSPEAK 304
Query: 564 KLI 572
+
Sbjct: 305 DFL 307
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 113 bits (282), Expect = 4e-25
Identities = 62/170 (36%), Positives = 95/170 (55%), Gaps = 3/170 (1%)
Frame = +3
Query: 18 RYLPILNHENIVKYFGMIETATKWKILMEWMEKGSLGDEIRSTGSLPDDKIARYCKDILQ 197
+ L L+H NIV+Y G + IL+E++ GS+ + G+ P+ + Y +L
Sbjct: 123 KLLKNLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRTYTNQLLL 182
Query: 198 AVAFLHHNKIIHRDIKPRNILIDNNHRCKLSICDISRL---LPNDECASTFWGTPNYWAP 368
+ +LH++ I+HRDIK NIL+DN KL+ S+ L A + GTP + AP
Sbjct: 183 GLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPYWMAP 242
Query: 369 EIFIGEPYNYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGTTR 518
E+ + +++ ADIWSVG TVI M+TG P+ + Y A I+ IGTT+
Sbjct: 243 EVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQ--QYKEIAAIFHIGTTK 290
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 112 bits (281), Expect = 6e-25
Identities = 63/166 (37%), Positives = 95/166 (57%), Gaps = 6/166 (3%)
Frame = +3
Query: 33 LNHENIVKYFGMIETATK--WKILMEWMEKGSLGDEIRSTGSLPDDKIARYCKDILQAVA 206
L HE IV+Y+G + + I ME+M GS+ D++++ G+L + +Y + IL+ ++
Sbjct: 419 LQHERIVQYYGCLRDRAEKILTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMS 478
Query: 207 FLHHNKIIHRDIKPRNILIDNNHRCKLSICDISRLLPNDECAST----FWGTPNYWAPEI 374
+LH N I+HRDIK NIL D+ KL S+ L + T GTP + +PE+
Sbjct: 479 YLHSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGIRSVTGTPYWMSPEV 538
Query: 375 FIGEPYNYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGT 512
GE Y AD+WS+G TV+ MLT PP+ E Y + A I++I T
Sbjct: 539 ISGEGYGRKADVWSLGCTVVEMLTEKPPWAE---YEAMAAIFKIAT 581
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 112 bits (281), Expect = 6e-25
Identities = 63/166 (37%), Positives = 95/166 (57%), Gaps = 6/166 (3%)
Frame = +3
Query: 33 LNHENIVKYFGMIETATK--WKILMEWMEKGSLGDEIRSTGSLPDDKIARYCKDILQAVA 206
L HE IV+Y+G + + I ME+M GS+ D++++ G+L + +Y + IL+ ++
Sbjct: 419 LQHERIVQYYGCLRDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMS 478
Query: 207 FLHHNKIIHRDIKPRNILIDNNHRCKLSICDISRLLPNDECAST----FWGTPNYWAPEI 374
+LH N I+HRDIK NIL D+ KL S+ L + T GTP + +PE+
Sbjct: 479 YLHSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVTGTPYWMSPEV 538
Query: 375 FIGEPYNYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGT 512
GE Y AD+WS+G TV+ MLT PP+ E Y + A I++I T
Sbjct: 539 ISGEGYGRKADVWSLGCTVVEMLTEKPPWAE---YEAMAAIFKIAT 581
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 112 bits (279), Expect = 1e-24
Identities = 62/170 (36%), Positives = 95/170 (55%), Gaps = 3/170 (1%)
Frame = +3
Query: 18 RYLPILNHENIVKYFGMIETATKWKILMEWMEKGSLGDEIRSTGSLPDDKIARYCKDILQ 197
+ L L+H NIV+Y G + IL+E++ GS+ + G P+ + Y + +L
Sbjct: 124 KLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYTRQLLL 183
Query: 198 AVAFLHHNKIIHRDIKPRNILIDNNHRCKLSICDISRL---LPNDECASTFWGTPNYWAP 368
+ +LH++ I+HRDIK NIL+DN KL+ S+ L A + GTP + AP
Sbjct: 184 GLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTPYWMAP 243
Query: 369 EIFIGEPYNYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGTTR 518
E+ + +++ ADIWSVG TVI M+TG P+ + Y A I+ IGTT+
Sbjct: 244 EVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQ--QYKEVAAIFFIGTTK 291
>sp|O95382|M3K6_HUMAN Mitogen-activated protein kinase kinase kinase 6
Length = 1011
Score = 110 bits (274), Expect = 4e-24
Identities = 77/188 (40%), Positives = 99/188 (52%), Gaps = 8/188 (4%)
Frame = +3
Query: 33 LNHENIVKYFGMIETATKWKILMEWMEKGSLGDEIRST-GSLPDDK--IARYCKDILQAV 203
L H+NIV+Y G KI ME + GSL +RS G L D++ I+ Y + ILQ +
Sbjct: 423 LRHKNIVRYLGSASQGGYLKIFMEEVPGGSLSSLLRSVWGPLKDNESTISFYTRQILQGL 482
Query: 204 AFLHHNKIIHRDIKPRNILIDNNHRCKLSICDI---SRLLPNDECASTFWGTPNYWAPEI 374
+LH N I+HRDIK N+LI N L I D RL C TF GT Y APEI
Sbjct: 483 GYLHDNHIVHRDIKGDNVLI-NTFSGLLKISDFGTSKRLAGITPCTETFTGTLQYMAPEI 541
Query: 375 FIGEP--YNYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGTTRKLNYTPPRQI 548
P Y ADIWS+G TVI M TG PP+ EL A ++++G K++ P +
Sbjct: 542 IDQGPRGYGKAADIWSLGCTVIEMATGRPPFHELGS--PQAAMFQVG-MYKVHPPMPSSL 598
Query: 549 SNEMQKLI 572
S E Q +
Sbjct: 599 SAEAQAFL 606
>sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 618
Score = 109 bits (273), Expect = 5e-24
Identities = 64/173 (36%), Positives = 95/173 (54%), Gaps = 6/173 (3%)
Frame = +3
Query: 39 HENIVKYFGMIETATK--WKILMEWMEKGSLGDEIRSTGSLPDDKIARYCKDILQAVAFL 212
HE IV+Y+G + + I ME+M GS+ D++++ G+L ++ +Y + IL+ V +L
Sbjct: 414 HERIVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENGTRKYTRQILEGVHYL 473
Query: 213 HHNKIIHRDIKPRNILIDNNHRCKLSICDISRLLPNDECAST----FWGTPNYWAPEIFI 380
H N I+HRDIK NIL D+ KL S+ L + T GTP + +PE+
Sbjct: 474 HSNMILHRDIKGANILRDSTGNVKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEVIS 533
Query: 381 GEPYNYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGTTRKLNYTPP 539
G+ Y ADIWSV TV+ MLT PP+ E + + A I++I T PP
Sbjct: 534 GQGYGRKADIWSVACTVVEMLTEKPPWAE---FEAMAAIFKIATQPTNPKLPP 583
>sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinase kinase 5 (MAPK/ERK kinase
kinase 5) (MEK kinase 5) (MEKK 5) (Apoptosis
signal-regulating kinase 1) (ASK-1)
Length = 1374
Score = 108 bits (271), Expect = 8e-24
Identities = 76/188 (40%), Positives = 101/188 (53%), Gaps = 8/188 (4%)
Frame = +3
Query: 33 LNHENIVKYFGMIETATKWKILMEWMEKGSLGDEIRST-GSLPDDK--IARYCKDILQAV 203
L H+NIV+Y G KI ME + GSL +RS G L D++ I Y K IL+ +
Sbjct: 732 LKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGL 791
Query: 204 AFLHHNKIIHRDIKPRNILIDNNHRCKLSICDI---SRLLPNDECASTFWGTPNYWAPEI 374
+LH N+I+HRDIK N+LI N + L I D RL + C TF GT Y APEI
Sbjct: 792 KYLHDNQIVHRDIKGDNVLI-NTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEI 850
Query: 375 FIGEP--YNYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGTTRKLNYTPPRQI 548
P Y ADIWS+G T+I M TG PP+ EL A ++++G K++ P +
Sbjct: 851 IDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGE--PQAAMFKVGMF-KVHPEIPESM 907
Query: 549 SNEMQKLI 572
S E + I
Sbjct: 908 SAEAKAFI 915
>sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 619
Score = 107 bits (268), Expect = 2e-23
Identities = 65/175 (37%), Positives = 94/175 (53%), Gaps = 6/175 (3%)
Frame = +3
Query: 33 LNHENIVKYFGMIETATK--WKILMEWMEKGSLGDEIRSTGSLPDDKIARYCKDILQAVA 206
L HE IV+Y+G + + I ME GS+ D++++ G+L ++ +Y + IL+ V
Sbjct: 413 LLHERIVQYYGCLRDPQEKTLSIFMELSPGGSIKDQLKAYGALTENVTRKYTRQILEGVH 472
Query: 207 FLHHNKIIHRDIKPRNILIDNNHRCKLSICDISRLLPNDECAST----FWGTPNYWAPEI 374
+LH N I+HRDIK NIL D+ KL S+ L + T GTP + +PE+
Sbjct: 473 YLHSNMIVHRDIKGANILRDSTGNIKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEV 532
Query: 375 FIGEPYNYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGTTRKLNYTPP 539
GE Y ADIWSV TV+ MLT PP+ E + + A I++I T PP
Sbjct: 533 ISGEGYGRKADIWSVACTVVEMLTEKPPWAE---FEAMAAIFKIATQPTNPKLPP 584
>sp|Q12263|GIN4_YEAST Serine/threonine-protein kinase GIN4
Length = 1142
Score = 104 bits (259), Expect = 2e-22
Identities = 59/186 (31%), Positives = 103/186 (55%), Gaps = 3/186 (1%)
Frame = +3
Query: 24 LPILNHENIVKYFGMIETATKWKILMEWMEKGSLGDEIRSTGSLPDDKIARYCKDILQAV 203
+ +LNH N+++ + + ET T +++E+ EKG L + + G LP+ + R+ + I+ V
Sbjct: 85 MKLLNHPNVLRLYDVWETNTDLYLVLEYAEKGELFNLLVERGPLPEHEAIRFFRQIIIGV 144
Query: 204 AFLHHNKIIHRDIKPRNILIDNNHRCKLSICDISRLLPNDECASTFWGTPNYWAPEIFIG 383
++ H I+HRD+KP N+L+D+ + K++ ++ L + T G+P+Y APEI G
Sbjct: 145 SYCHALGIVHRDLKPENLLLDHKYNIKIADFGMAALETEGKLLETSCGSPHYAAPEIVSG 204
Query: 384 EPY-NYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGTTRKLNYTPPR--QISN 554
PY + +D+WS G + +LTG P+ E I ++ +K + P +IS
Sbjct: 205 IPYQGFASDVWSCGVILFALLTGRLPFDEEDGNIRTLLL----KVQKGEFEMPSDDEISR 260
Query: 555 EMQKLI 572
E Q LI
Sbjct: 261 EAQDLI 266
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83,751,526
Number of Sequences: 369166
Number of extensions: 1898138
Number of successful extensions: 8408
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6449
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6799
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 4926080070
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)