Planaria EST Database


DrC_02690

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_02690
         (624 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O22042|M3K3_ARATH  Mitogen-activated protein kinase kinas...   118   1e-26
sp|Q9FZ36|M3K2_ARATH  Mitogen-activated protein kinase kinas...   113   4e-25
sp|Q61084|M3K3_MOUSE  Mitogen-activated protein kinase kinas...   112   6e-25
sp|Q99759|M3K3_HUMAN  Mitogen-activated protein kinase kinas...   112   6e-25
sp|O22040|M3K1_ARATH  Mitogen-activated protein kinase kinas...   112   1e-24
sp|O95382|M3K6_HUMAN  Mitogen-activated protein kinase kinas...   110   4e-24
sp|Q9Y2U5|M3K2_HUMAN  Mitogen-activated protein kinase kinas...   109   5e-24
sp|Q99683|M3K5_HUMAN  Mitogen-activated protein kinase kinas...   108   8e-24
sp|Q61083|M3K2_MOUSE  Mitogen-activated protein kinase kinas...   107   2e-23
sp|Q12263|GIN4_YEAST  Serine/threonine-protein kinase GIN4        104   2e-22
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
           NPK1-related protein kinase 3)
          Length = 651

 Score =  118 bits (295), Expect = 1e-26
 Identities = 69/183 (37%), Positives = 100/183 (54%), Gaps = 3/183 (1%)
 Frame = +3

Query: 33  LNHENIVKYFGMIETATKWKILMEWMEKGSLGDEIRSTGSLPDDKIARYCKDILQAVAFL 212
           L+H NIV+Y G +  +    ILME++  GS+   +   GS P+  I  Y K +L  + +L
Sbjct: 128 LSHPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQLLLGLEYL 187

Query: 213 HHNKIIHRDIKPRNILIDNNHRCKLSICDISRL---LPNDECASTFWGTPNYWAPEIFIG 383
           H+N I+HRDIK  NIL+DN    +L+    S+    L     A +  GTP + APE+ + 
Sbjct: 188 HNNGIMHRDIKGANILVDNKGCIRLADFGASKKVVELATVNGAKSMKGTPYWMAPEVILQ 247

Query: 384 EPYNYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGTTRKLNYTPPRQISNEMQ 563
             +++ ADIWSVG TVI M TG PP+ E   Y   A +  IG T K +   P  +S E +
Sbjct: 248 TGHSFSADIWSVGCTVIEMATGKPPWSE--QYQQFAAVLHIGRT-KAHPPIPEDLSPEAK 304

Query: 564 KLI 572
             +
Sbjct: 305 DFL 307
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
           NPK1-related protein kinase 2)
          Length = 651

 Score =  113 bits (282), Expect = 4e-25
 Identities = 62/170 (36%), Positives = 95/170 (55%), Gaps = 3/170 (1%)
 Frame = +3

Query: 18  RYLPILNHENIVKYFGMIETATKWKILMEWMEKGSLGDEIRSTGSLPDDKIARYCKDILQ 197
           + L  L+H NIV+Y G +       IL+E++  GS+   +   G+ P+  +  Y   +L 
Sbjct: 123 KLLKNLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRTYTNQLLL 182

Query: 198 AVAFLHHNKIIHRDIKPRNILIDNNHRCKLSICDISRL---LPNDECASTFWGTPNYWAP 368
            + +LH++ I+HRDIK  NIL+DN    KL+    S+    L     A +  GTP + AP
Sbjct: 183 GLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAELATISGAKSMKGTPYWMAP 242

Query: 369 EIFIGEPYNYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGTTR 518
           E+ +   +++ ADIWSVG TVI M+TG  P+ +   Y   A I+ IGTT+
Sbjct: 243 EVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQ--QYKEIAAIFHIGTTK 290
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
           kinase 3) (MEK kinase 3) (MEKK 3)
          Length = 626

 Score =  112 bits (281), Expect = 6e-25
 Identities = 63/166 (37%), Positives = 95/166 (57%), Gaps = 6/166 (3%)
 Frame = +3

Query: 33  LNHENIVKYFGMIETATK--WKILMEWMEKGSLGDEIRSTGSLPDDKIARYCKDILQAVA 206
           L HE IV+Y+G +    +    I ME+M  GS+ D++++ G+L +    +Y + IL+ ++
Sbjct: 419 LQHERIVQYYGCLRDRAEKILTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMS 478

Query: 207 FLHHNKIIHRDIKPRNILIDNNHRCKLSICDISRLLPNDECAST----FWGTPNYWAPEI 374
           +LH N I+HRDIK  NIL D+    KL     S+ L     + T      GTP + +PE+
Sbjct: 479 YLHSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGIRSVTGTPYWMSPEV 538

Query: 375 FIGEPYNYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGT 512
             GE Y   AD+WS+G TV+ MLT  PP+ E   Y + A I++I T
Sbjct: 539 ISGEGYGRKADVWSLGCTVVEMLTEKPPWAE---YEAMAAIFKIAT 581
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
           kinase 3) (MEK kinase 3) (MEKK 3)
          Length = 626

 Score =  112 bits (281), Expect = 6e-25
 Identities = 63/166 (37%), Positives = 95/166 (57%), Gaps = 6/166 (3%)
 Frame = +3

Query: 33  LNHENIVKYFGMIETATK--WKILMEWMEKGSLGDEIRSTGSLPDDKIARYCKDILQAVA 206
           L HE IV+Y+G +    +    I ME+M  GS+ D++++ G+L +    +Y + IL+ ++
Sbjct: 419 LQHERIVQYYGCLRDRAEKTLTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMS 478

Query: 207 FLHHNKIIHRDIKPRNILIDNNHRCKLSICDISRLLPNDECAST----FWGTPNYWAPEI 374
           +LH N I+HRDIK  NIL D+    KL     S+ L     + T      GTP + +PE+
Sbjct: 479 YLHSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMSGTGMRSVTGTPYWMSPEV 538

Query: 375 FIGEPYNYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGT 512
             GE Y   AD+WS+G TV+ MLT  PP+ E   Y + A I++I T
Sbjct: 539 ISGEGYGRKADVWSLGCTVVEMLTEKPPWAE---YEAMAAIFKIAT 581
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
           NPK1-related protein kinase 1)
          Length = 666

 Score =  112 bits (279), Expect = 1e-24
 Identities = 62/170 (36%), Positives = 95/170 (55%), Gaps = 3/170 (1%)
 Frame = +3

Query: 18  RYLPILNHENIVKYFGMIETATKWKILMEWMEKGSLGDEIRSTGSLPDDKIARYCKDILQ 197
           + L  L+H NIV+Y G +       IL+E++  GS+   +   G  P+  +  Y + +L 
Sbjct: 124 KLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYTRQLLL 183

Query: 198 AVAFLHHNKIIHRDIKPRNILIDNNHRCKLSICDISRL---LPNDECASTFWGTPNYWAP 368
            + +LH++ I+HRDIK  NIL+DN    KL+    S+    L     A +  GTP + AP
Sbjct: 184 GLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAELATMTGAKSMKGTPYWMAP 243

Query: 369 EIFIGEPYNYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGTTR 518
           E+ +   +++ ADIWSVG TVI M+TG  P+ +   Y   A I+ IGTT+
Sbjct: 244 EVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQ--QYKEVAAIFFIGTTK 291
>sp|O95382|M3K6_HUMAN Mitogen-activated protein kinase kinase kinase 6
          Length = 1011

 Score =  110 bits (274), Expect = 4e-24
 Identities = 77/188 (40%), Positives = 99/188 (52%), Gaps = 8/188 (4%)
 Frame = +3

Query: 33  LNHENIVKYFGMIETATKWKILMEWMEKGSLGDEIRST-GSLPDDK--IARYCKDILQAV 203
           L H+NIV+Y G        KI ME +  GSL   +RS  G L D++  I+ Y + ILQ +
Sbjct: 423 LRHKNIVRYLGSASQGGYLKIFMEEVPGGSLSSLLRSVWGPLKDNESTISFYTRQILQGL 482

Query: 204 AFLHHNKIIHRDIKPRNILIDNNHRCKLSICDI---SRLLPNDECASTFWGTPNYWAPEI 374
            +LH N I+HRDIK  N+LI N     L I D     RL     C  TF GT  Y APEI
Sbjct: 483 GYLHDNHIVHRDIKGDNVLI-NTFSGLLKISDFGTSKRLAGITPCTETFTGTLQYMAPEI 541

Query: 375 FIGEP--YNYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGTTRKLNYTPPRQI 548
               P  Y   ADIWS+G TVI M TG PP+ EL      A ++++G   K++   P  +
Sbjct: 542 IDQGPRGYGKAADIWSLGCTVIEMATGRPPFHELGS--PQAAMFQVG-MYKVHPPMPSSL 598

Query: 549 SNEMQKLI 572
           S E Q  +
Sbjct: 599 SAEAQAFL 606
>sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
           kinase 2) (MEK kinase 2) (MEKK 2)
          Length = 618

 Score =  109 bits (273), Expect = 5e-24
 Identities = 64/173 (36%), Positives = 95/173 (54%), Gaps = 6/173 (3%)
 Frame = +3

Query: 39  HENIVKYFGMIETATK--WKILMEWMEKGSLGDEIRSTGSLPDDKIARYCKDILQAVAFL 212
           HE IV+Y+G +    +    I ME+M  GS+ D++++ G+L ++   +Y + IL+ V +L
Sbjct: 414 HERIVQYYGCLRDPQEKTLSIFMEYMPGGSIKDQLKAYGALTENGTRKYTRQILEGVHYL 473

Query: 213 HHNKIIHRDIKPRNILIDNNHRCKLSICDISRLLPNDECAST----FWGTPNYWAPEIFI 380
           H N I+HRDIK  NIL D+    KL     S+ L     + T      GTP + +PE+  
Sbjct: 474 HSNMILHRDIKGANILRDSTGNVKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEVIS 533

Query: 381 GEPYNYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGTTRKLNYTPP 539
           G+ Y   ADIWSV  TV+ MLT  PP+ E   + + A I++I T       PP
Sbjct: 534 GQGYGRKADIWSVACTVVEMLTEKPPWAE---FEAMAAIFKIATQPTNPKLPP 583
>sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinase kinase 5 (MAPK/ERK kinase
            kinase 5) (MEK kinase 5) (MEKK 5) (Apoptosis
            signal-regulating kinase 1) (ASK-1)
          Length = 1374

 Score =  108 bits (271), Expect = 8e-24
 Identities = 76/188 (40%), Positives = 101/188 (53%), Gaps = 8/188 (4%)
 Frame = +3

Query: 33   LNHENIVKYFGMIETATKWKILMEWMEKGSLGDEIRST-GSLPDDK--IARYCKDILQAV 203
            L H+NIV+Y G        KI ME +  GSL   +RS  G L D++  I  Y K IL+ +
Sbjct: 732  LKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGL 791

Query: 204  AFLHHNKIIHRDIKPRNILIDNNHRCKLSICDI---SRLLPNDECASTFWGTPNYWAPEI 374
             +LH N+I+HRDIK  N+LI N +   L I D     RL   + C  TF GT  Y APEI
Sbjct: 792  KYLHDNQIVHRDIKGDNVLI-NTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEI 850

Query: 375  FIGEP--YNYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGTTRKLNYTPPRQI 548
                P  Y   ADIWS+G T+I M TG PP+ EL      A ++++G   K++   P  +
Sbjct: 851  IDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGE--PQAAMFKVGMF-KVHPEIPESM 907

Query: 549  SNEMQKLI 572
            S E +  I
Sbjct: 908  SAEAKAFI 915
>sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
           kinase 2) (MEK kinase 2) (MEKK 2)
          Length = 619

 Score =  107 bits (268), Expect = 2e-23
 Identities = 65/175 (37%), Positives = 94/175 (53%), Gaps = 6/175 (3%)
 Frame = +3

Query: 33  LNHENIVKYFGMIETATK--WKILMEWMEKGSLGDEIRSTGSLPDDKIARYCKDILQAVA 206
           L HE IV+Y+G +    +    I ME    GS+ D++++ G+L ++   +Y + IL+ V 
Sbjct: 413 LLHERIVQYYGCLRDPQEKTLSIFMELSPGGSIKDQLKAYGALTENVTRKYTRQILEGVH 472

Query: 207 FLHHNKIIHRDIKPRNILIDNNHRCKLSICDISRLLPNDECAST----FWGTPNYWAPEI 374
           +LH N I+HRDIK  NIL D+    KL     S+ L     + T      GTP + +PE+
Sbjct: 473 YLHSNMIVHRDIKGANILRDSTGNIKLGDFGASKRLQTICLSGTGMKSVTGTPYWMSPEV 532

Query: 375 FIGEPYNYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGTTRKLNYTPP 539
             GE Y   ADIWSV  TV+ MLT  PP+ E   + + A I++I T       PP
Sbjct: 533 ISGEGYGRKADIWSVACTVVEMLTEKPPWAE---FEAMAAIFKIATQPTNPKLPP 584
>sp|Q12263|GIN4_YEAST Serine/threonine-protein kinase GIN4
          Length = 1142

 Score =  104 bits (259), Expect = 2e-22
 Identities = 59/186 (31%), Positives = 103/186 (55%), Gaps = 3/186 (1%)
 Frame = +3

Query: 24  LPILNHENIVKYFGMIETATKWKILMEWMEKGSLGDEIRSTGSLPDDKIARYCKDILQAV 203
           + +LNH N+++ + + ET T   +++E+ EKG L + +   G LP+ +  R+ + I+  V
Sbjct: 85  MKLLNHPNVLRLYDVWETNTDLYLVLEYAEKGELFNLLVERGPLPEHEAIRFFRQIIIGV 144

Query: 204 AFLHHNKIIHRDIKPRNILIDNNHRCKLSICDISRLLPNDECASTFWGTPNYWAPEIFIG 383
           ++ H   I+HRD+KP N+L+D+ +  K++   ++ L    +   T  G+P+Y APEI  G
Sbjct: 145 SYCHALGIVHRDLKPENLLLDHKYNIKIADFGMAALETEGKLLETSCGSPHYAAPEIVSG 204

Query: 384 EPY-NYPADIWSVGATVIHMLTGHPPYLELRPYISAAVIYRIGTTRKLNYTPPR--QISN 554
            PY  + +D+WS G  +  +LTG  P+ E    I   ++      +K  +  P   +IS 
Sbjct: 205 IPYQGFASDVWSCGVILFALLTGRLPFDEEDGNIRTLLL----KVQKGEFEMPSDDEISR 260

Query: 555 EMQKLI 572
           E Q LI
Sbjct: 261 EAQDLI 266
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83,751,526
Number of Sequences: 369166
Number of extensions: 1898138
Number of successful extensions: 8408
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6449
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6799
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 4926080070
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)