Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_02519
(758 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q01241|NF70_LOLPE 70 kDa neurofilament protein (NF70) 69 1e-11
sp|Q01240|NF60_LOLPE 60 kDa neurofilament protein (NF60) 69 1e-11
sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (I... 68 3e-11
sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (I... 62 1e-09
sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (I... 62 2e-09
sp|Q03427|LAMC_DROME Lamin C (pG-IF) 58 2e-08
sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (I... 58 3e-08
sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (I... 57 7e-08
sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B) 56 1e-07
sp|P35617|XNIF_XENLA Low molecular weight neuronal intermed... 54 3e-07
>sp|Q01241|NF70_LOLPE 70 kDa neurofilament protein (NF70)
Length = 615
Score = 68.9 bits (167), Expect = 1e-11
Identities = 45/191 (23%), Positives = 94/191 (49%), Gaps = 8/191 (4%)
Frame = +3
Query: 126 SPNGVHSLEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLINEIRKIKGE------N 287
S GV+ ++ + ++K+ + ++NE A YIEKV+ LE QN+ L E+ ++K +
Sbjct: 82 SSTGVNHVKANREREKQDMRDLNERFANYIEKVRFLEAQNKKLAGELEELKSKWGKETSA 141
Query: 288 VQEIYQKEIDQIQSLLKKSETHKEPMKIQIDSFKNKIVEMQKRLVVLEDINSQYRETIED 467
++E+Y+ E+++ + L+ + K + +++ +++ QK L + +E I
Sbjct: 142 IKEMYETELEEARKLIDATNKEKITLDVRVTELIDQLERQQKDLEESRTYHQIDQEQIAR 201
Query: 468 QRGXXXXXXXXXXXXKKFKAIQDLDSEKTKQET--ATLYDEITKLRENYEKEVTAQITLE 641
Q + ++I+ L+ EK +Q A + DE+ K+R + E + E
Sbjct: 202 QNQQLADLEGEISMLR--RSIESLEKEKMRQSNILAKMNDEMEKMRMDLNNETINHLDAE 259
Query: 642 HNIKTFKETKE 674
+ +T +E E
Sbjct: 260 NRRQTLEEELE 270
>sp|Q01240|NF60_LOLPE 60 kDa neurofilament protein (NF60)
Length = 511
Score = 68.9 bits (167), Expect = 1e-11
Identities = 45/191 (23%), Positives = 94/191 (49%), Gaps = 8/191 (4%)
Frame = +3
Query: 126 SPNGVHSLEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLINEIRKIKGE------N 287
S GV+ ++ + ++K+ + ++NE A YIEKV+ LE QN+ L E+ ++K +
Sbjct: 82 SSTGVNHVKANREREKQDMRDLNERFANYIEKVRFLEAQNKKLAGELEELKSKWGKETSA 141
Query: 288 VQEIYQKEIDQIQSLLKKSETHKEPMKIQIDSFKNKIVEMQKRLVVLEDINSQYRETIED 467
++E+Y+ E+++ + L+ + K + +++ +++ QK L + +E I
Sbjct: 142 IKEMYETELEEARKLIDATNKEKITLDVRVTELIDQLERQQKDLEESRTYHQIDQEQIAR 201
Query: 468 QRGXXXXXXXXXXXXKKFKAIQDLDSEKTKQET--ATLYDEITKLRENYEKEVTAQITLE 641
Q + ++I+ L+ EK +Q A + DE+ K+R + E + E
Sbjct: 202 QNQQLADLEGEISMLR--RSIESLEKEKMRQSNILAKMNDEMEKMRMDLNNETINHLDAE 259
Query: 642 HNIKTFKETKE 674
+ +T +E E
Sbjct: 260 NRRQTLEEELE 270
>sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (Intermediate filament protein
A1) (IF-A1) (Cel IF A1)
Length = 575
Score = 67.8 bits (164), Expect = 3e-11
Identities = 41/184 (22%), Positives = 91/184 (49%), Gaps = 6/184 (3%)
Frame = +3
Query: 132 NGVHSLEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLINEIRKIKGE------NVQ 293
N ++ ++KK + ++N+ +A YIEKV+ LE QNR L ++ ++ + N++
Sbjct: 57 NAASTIRDSREREKKEMSDLNDRLASYIEKVRFLEAQNRKLAADLDALRSKWGKDTHNIR 116
Query: 294 EIYQKEIDQIQSLLKKSETHKEPMKIQIDSFKNKIVEMQKRLVVLEDINSQYRETIEDQR 473
+Y+ E+ Q L+ ++ ++ M+ Q+ ++++ EM+++L Q R I+
Sbjct: 117 NMYEGELVDAQKLIDETNKQRKDMEGQLKKMQDELAEMRRKLEDATKGREQDRAKIDALL 176
Query: 474 GXXXXXXXXXXXXKKFKAIQDLDSEKTKQETATLYDEITKLRENYEKEVTAQITLEHNIK 653
K+ A + + ++ KQE L E+ + R + ++E +I ++ ++
Sbjct: 177 VTLSNLEAEISLLKRRIAQLEDEVKRIKQENQRLLSELQRARTDLDQETLNRIDYQNQVQ 236
Query: 654 TFKE 665
T E
Sbjct: 237 TLLE 240
Score = 34.7 bits (78), Expect = 0.29
Identities = 35/206 (16%), Positives = 82/206 (39%), Gaps = 19/206 (9%)
Frame = +3
Query: 111 IDNVDSPNGVHSLEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLINEIRKIKGENV 290
++ +D VH E ELQ S + E + ++ + R +++ + ++
Sbjct: 239 LEEIDFLRRVHDNEIKELQTLASRDTTPENREFFKNELSSAIRDIREEYDQVNNVHRNDM 298
Query: 291 QEIYQKEIDQIQSLLKKSETHKEPMKIQIDSFKNKIVEMQKRLVVLEDINSQYRETI--- 461
+ Y+ ++ +IQ+ + + K ++ + ++ +++ +L LE NS + I
Sbjct: 299 ESWYRLKVQEIQTQSARQNMEQGYAKEEVKRLRTQLSDLRGKLADLESRNSLLEKQIQEL 358
Query: 462 -----EDQRGXXXXXXXXXXXXKKFKAIQD---------LDSEKTKQETATLYDEITKLR 599
+DQR +K + LD+++T +Y ++ +
Sbjct: 359 NYQLEDDQRSYEAALNDRDSQIRKMREECQALMVELQMLLDTKQTLDAEIAIYRKMLEGE 418
Query: 600 ENYE--KEVTAQITLEHNIKTFKETK 671
EN K++ Q+ H I +T+
Sbjct: 419 ENRAGLKQLVEQVVKTHAITQETDTE 444
>sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (Intermediate filament protein
A2) (IF-A2) (Cel IF A2)
Length = 581
Score = 62.4 bits (150), Expect = 1e-09
Identities = 45/189 (23%), Positives = 96/189 (50%), Gaps = 11/189 (5%)
Frame = +3
Query: 132 NGVHSLEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLINEIRKIKGE------NVQ 293
N ++ + ++KK + E+N+ +A YIEKV+ L+ QNR L +++ ++G +V+
Sbjct: 59 NAASTIRDNREREKKEIMELNDRLASYIEKVRFLDAQNRKLDADLKMLQGRFGKSTGSVK 118
Query: 294 EIYQKEIDQIQSLLKKS-----ETHKEPMKIQIDSFKNKIVEMQKRLVVLEDINSQYRET 458
+Y+ EI +++K++ ET KE K+Q +++ E++K+ + ++ R
Sbjct: 119 VMYEMEITTATNVVKQTGKDHGETEKEIRKLQ-----DQLDELRKKFEEAQRGRAEDRLK 173
Query: 459 IEDQRGXXXXXXXXXXXXKKFKAIQDLDSEKTKQETATLYDEITKLRENYEKEVTAQITL 638
I+D K+ A+ + + + K+E L E+ ++R ++E +I
Sbjct: 174 IDDLLVTLSNLEAEINLLKRRIALLEEEVARLKKENFRLTSELQRVRIELDQETLLRIDN 233
Query: 639 EHNIKTFKE 665
++ +KT E
Sbjct: 234 QNKVKTILE 242
>sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (Intermediate filament protein
A4) (IF-A4) (Cel IF A4)
Length = 575
Score = 62.0 bits (149), Expect = 2e-09
Identities = 42/198 (21%), Positives = 93/198 (46%), Gaps = 9/198 (4%)
Frame = +3
Query: 99 GSNRIDNVDSPNGVHS---LEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLINEIR 269
G + + + SP G H+ + + ++KK + E+N+ +A YIEKV+ LE QNR + ++
Sbjct: 44 GGSNLTSGMSPFGGHAASAIRESRTREKKEMSELNDRLASYIEKVRFLEAQNRKMEKDLN 103
Query: 270 KIKGE------NVQEIYQKEIDQIQSLLKKSETHKEPMKIQIDSFKNKIVEMQKRLVVLE 431
++G+ +V+ +Y+ E+ + L+ S+ + ++ QI ++ + +L E
Sbjct: 104 LLRGKWGHDSTSVKVMYETELRSAKDLIADSDKERAQLEDQIKKLVEELNNYRNKLHEAE 163
Query: 432 DINSQYRETIEDQRGXXXXXXXXXXXXKKFKAIQDLDSEKTKQETATLYDEITKLRENYE 611
R+ +++ G K+ ++ + K+E + D + R E
Sbjct: 164 RGADVTRKELDNVLGRLGALEAEIELLKRRISLMEESIGHLKRENHRMIDSLHHARNAVE 223
Query: 612 KEVTAQITLEHNIKTFKE 665
+E +I ++ ++T E
Sbjct: 224 QETLNRIDYQNQVQTLLE 241
>sp|Q03427|LAMC_DROME Lamin C (pG-IF)
Length = 621
Score = 58.2 bits (139), Expect = 2e-08
Identities = 48/215 (22%), Positives = 96/215 (44%), Gaps = 26/215 (12%)
Frame = +3
Query: 99 GSNRIDNVDSPNGVHSLEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLINEIRKIK 278
G++ V + + Q+K+ L+ +N+ +A YI++++NLE +N L E+ +
Sbjct: 23 GASTSSRVGATSPTSPTRTSRQQEKEELQHLNDRLACYIDRMRNLENENSRLTQELNLAQ 82
Query: 279 G------ENVQEIYQKEIDQIQSLLKKSETHKEPMKIQI---------DSFKNKIVEMQK 413
N++ +Y+KE+ + LL ET KE K++I D K ++ + K
Sbjct: 83 DTVNRETSNLKAVYEKELAAARKLL--DETAKEKAKLEIDIKRLWEENDDLKPRLDKKTK 140
Query: 414 RLVVLE-----------DINSQYRETIEDQRGXXXXXXXXXXXXKKFKAIQDLDSEKTKQ 560
V E ++N +Y +++ D+ KKF+ +++
Sbjct: 141 EATVAENNARLYENRYNEVNGKYNQSLADR--------------KKFED----QAKELAL 182
Query: 561 ETATLYDEITKLRENYEKEVTAQITLEHNIKTFKE 665
E L ++ LR+ E E A++ LE+ ++ +E
Sbjct: 183 ENERLRRQLDDLRKQLEAETLARVDLENQNQSLRE 217
>sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (Intermediate filament protein
B1) (IF-B1) (Cel IF B1)
Length = 589
Score = 57.8 bits (138), Expect = 3e-08
Identities = 40/190 (21%), Positives = 89/190 (46%), Gaps = 7/190 (3%)
Frame = +3
Query: 126 SPNGVHSLEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLI---NEIRKIKGENVQE 296
S N S + ++KK+L+ +N+ + YI++VK LE QNR L+ +E+R G++ E
Sbjct: 67 SANAAKSFLEATDKEKKTLQGLNDRLGNYIDRVKKLEEQNRKLVADLDELRGKWGKDTSE 126
Query: 297 I---YQKEIDQIQSLLKKSETHKEPMKIQIDSFKNKIVEMQKRLVVLEDINSQYRETIED 467
I Y + + + + + K + +++ ++ + E + R ++ RE I
Sbjct: 127 IKIKYSESLSTARKDIDDAARRKAEVDVKVARHRDDLAEYRSRYEDIQQRRESDREKISQ 186
Query: 468 QRGXXXXXXXXXXXXK-KFKAIQDLDSEKTKQETATLYDEITKLRENYEKEVTAQITLEH 644
+ +FK + D + ++ + + +++E+ K R + + E +I ++
Sbjct: 187 WTNAIADAQSEVEMLRARFKQLTD-EEKRVTADNSRIWEELQKARSDLDDETIGRIDFQN 245
Query: 645 NIKTFKETKE 674
++T E E
Sbjct: 246 QVQTLMEELE 255
>sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (Intermediate filament protein
A3) (IF-A3) (Cel IF A3)
Length = 581
Score = 56.6 bits (135), Expect = 7e-08
Identities = 38/180 (21%), Positives = 88/180 (48%), Gaps = 6/180 (3%)
Frame = +3
Query: 144 SLEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLINEIRKIKGE------NVQEIYQ 305
++ D ++KK + E+N+ +A YI KV+ L QNR L ++ ++ +V+ +Y+
Sbjct: 63 TIRDDREREKKEITELNDRLASYIGKVRFLAAQNRKLEADLNVLQSRFGKSTGSVKIMYE 122
Query: 306 KEIDQIQSLLKKSETHKEPMKIQIDSFKNKIVEMQKRLVVLEDINSQYRETIEDQRGXXX 485
EI +++K++ E + +I K+++ E++K+ + ++ R I++
Sbjct: 123 MEITTATNVVKETGKDHEEAEKEIGKIKDQLDELRKKFEEAQKGRAEDRLKIDELLVTLS 182
Query: 486 XXXXXXXXXKKFKAIQDLDSEKTKQETATLYDEITKLRENYEKEVTAQITLEHNIKTFKE 665
K+ A+ + + + K+E L E+ ++R ++E +I ++ + T E
Sbjct: 183 NLEAEINLLKRRIALLEEEVARLKKENFRLTSELQRVRSELDQETLLRIDNQNKVTTILE 242
>sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B)
Length = 589
Score = 55.8 bits (133), Expect = 1e-07
Identities = 39/190 (20%), Positives = 91/190 (47%), Gaps = 7/190 (3%)
Frame = +3
Query: 126 SPNGVHSLEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLI---NEIRKIKGENVQE 296
S N S + ++KK ++ +N+ + YI++VK LE QNR L+ +E+R G++ E
Sbjct: 67 SANAAKSFLEATDKEKKEMQGLNDRLGNYIDRVKKLEEQNRKLVADLDELRGRWGKDTSE 126
Query: 297 IYQKEIDQIQSLLKKSE---THKEPMKIQIDSFKNKIVEMQKRLVVLEDINSQYRETIED 467
I + D ++ K+ + K + +++ ++ + E++ R ++ RE I
Sbjct: 127 IKIQYSDSLRDARKEIDDGARRKAEIDVKVARLRDDLAELRNRYEDVQHRRESDREKINQ 186
Query: 468 -QRGXXXXXXXXXXXXKKFKAIQDLDSEKTKQETATLYDEITKLRENYEKEVTAQITLEH 644
Q +++ + + + ++ + A +++E+ K R + ++E +I ++
Sbjct: 187 WQHAIEDAQSELEMLRARWRQLTE-EEKRLNGDNARIWEELQKARNDLDEETLGRIDFQN 245
Query: 645 NIKTFKETKE 674
++T E E
Sbjct: 246 QVQTLMEELE 255
>sp|P35617|XNIF_XENLA Low molecular weight neuronal intermediate filament (XNIF)
Length = 470
Score = 54.3 bits (129), Expect = 3e-07
Identities = 42/181 (23%), Positives = 88/181 (48%), Gaps = 20/181 (11%)
Frame = +3
Query: 168 KKKSLEEINEGIAIYIEKVKNLEIQNRNLINEIRKIKGEN-----VQEIYQKEIDQIQSL 332
+K+ L+ +N+ YIEKV +LE QN+ L +E+ ++ ++ + IY++EI +++S
Sbjct: 88 EKEQLQGLNDRFVTYIEKVHHLEQQNKLLESEVTLLRQKHSEPSRLSHIYEQEIRELRSK 147
Query: 333 LKKSETHKEPMKIQ-------IDSFKNKIVEMQKRLVVLEDINSQYRETIEDQRGXXXXX 491
L++ E K+ ++ ++ K K+ + R ED+ YR+ ++
Sbjct: 148 LEEQEQDKDQAQLDYEHLGACLEQLKLKLEQESARREEAEDVMKNYRKDLDQ-------- 199
Query: 492 XXXXXXXKKFKAIQDLDSEKTKQETATLYDEITKLRENYEKEVT--------AQITLEHN 647
L+ + +++ +L DEI LR+ +E+E+ AQI++E +
Sbjct: 200 -------------ATLNRLQLEKKVESLLDEIAFLRKVHEEEIAELQASVQEAQISVEMD 246
Query: 648 I 650
+
Sbjct: 247 V 247
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,446,858
Number of Sequences: 369166
Number of extensions: 913823
Number of successful extensions: 4719
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4226
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4643
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6970118400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)