Planaria EST Database


DrC_02519

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_02519
         (758 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q01241|NF70_LOLPE  70 kDa neurofilament protein (NF70)          69   1e-11
sp|Q01240|NF60_LOLPE  60 kDa neurofilament protein (NF60)          69   1e-11
sp|P90901|IFA1_CAEEL  Intermediate filament protein ifa-1 (I...    68   3e-11
sp|O02365|IFA2_CAEEL  Intermediate filament protein ifa-2 (I...    62   1e-09
sp|P90900|IFA4_CAEEL  Intermediate filament protein ifa-4 (I...    62   2e-09
sp|Q03427|LAMC_DROME  Lamin C (pG-IF)                              58   2e-08
sp|Q19289|IFB1_CAEEL  Intermediate filament protein ifb-1 (I...    58   3e-08
sp|Q21065|IFA3_CAEEL  Intermediate filament protein ifa-3 (I...    57   7e-08
sp|P23731|IFEB_ASCSU  Intermediate filament protein B (IF-B)       56   1e-07
sp|P35617|XNIF_XENLA  Low molecular weight neuronal intermed...    54   3e-07
>sp|Q01241|NF70_LOLPE 70 kDa neurofilament protein (NF70)
          Length = 615

 Score = 68.9 bits (167), Expect = 1e-11
 Identities = 45/191 (23%), Positives = 94/191 (49%), Gaps = 8/191 (4%)
 Frame = +3

Query: 126 SPNGVHSLEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLINEIRKIKGE------N 287
           S  GV+ ++ +  ++K+ + ++NE  A YIEKV+ LE QN+ L  E+ ++K +       
Sbjct: 82  SSTGVNHVKANREREKQDMRDLNERFANYIEKVRFLEAQNKKLAGELEELKSKWGKETSA 141

Query: 288 VQEIYQKEIDQIQSLLKKSETHKEPMKIQIDSFKNKIVEMQKRLVVLEDINSQYRETIED 467
           ++E+Y+ E+++ + L+  +   K  + +++    +++   QK L      +   +E I  
Sbjct: 142 IKEMYETELEEARKLIDATNKEKITLDVRVTELIDQLERQQKDLEESRTYHQIDQEQIAR 201

Query: 468 QRGXXXXXXXXXXXXKKFKAIQDLDSEKTKQET--ATLYDEITKLRENYEKEVTAQITLE 641
           Q              +  ++I+ L+ EK +Q    A + DE+ K+R +   E    +  E
Sbjct: 202 QNQQLADLEGEISMLR--RSIESLEKEKMRQSNILAKMNDEMEKMRMDLNNETINHLDAE 259

Query: 642 HNIKTFKETKE 674
           +  +T +E  E
Sbjct: 260 NRRQTLEEELE 270
>sp|Q01240|NF60_LOLPE 60 kDa neurofilament protein (NF60)
          Length = 511

 Score = 68.9 bits (167), Expect = 1e-11
 Identities = 45/191 (23%), Positives = 94/191 (49%), Gaps = 8/191 (4%)
 Frame = +3

Query: 126 SPNGVHSLEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLINEIRKIKGE------N 287
           S  GV+ ++ +  ++K+ + ++NE  A YIEKV+ LE QN+ L  E+ ++K +       
Sbjct: 82  SSTGVNHVKANREREKQDMRDLNERFANYIEKVRFLEAQNKKLAGELEELKSKWGKETSA 141

Query: 288 VQEIYQKEIDQIQSLLKKSETHKEPMKIQIDSFKNKIVEMQKRLVVLEDINSQYRETIED 467
           ++E+Y+ E+++ + L+  +   K  + +++    +++   QK L      +   +E I  
Sbjct: 142 IKEMYETELEEARKLIDATNKEKITLDVRVTELIDQLERQQKDLEESRTYHQIDQEQIAR 201

Query: 468 QRGXXXXXXXXXXXXKKFKAIQDLDSEKTKQET--ATLYDEITKLRENYEKEVTAQITLE 641
           Q              +  ++I+ L+ EK +Q    A + DE+ K+R +   E    +  E
Sbjct: 202 QNQQLADLEGEISMLR--RSIESLEKEKMRQSNILAKMNDEMEKMRMDLNNETINHLDAE 259

Query: 642 HNIKTFKETKE 674
           +  +T +E  E
Sbjct: 260 NRRQTLEEELE 270
>sp|P90901|IFA1_CAEEL Intermediate filament protein ifa-1 (Intermediate filament protein
           A1) (IF-A1) (Cel IF A1)
          Length = 575

 Score = 67.8 bits (164), Expect = 3e-11
 Identities = 41/184 (22%), Positives = 91/184 (49%), Gaps = 6/184 (3%)
 Frame = +3

Query: 132 NGVHSLEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLINEIRKIKGE------NVQ 293
           N   ++     ++KK + ++N+ +A YIEKV+ LE QNR L  ++  ++ +      N++
Sbjct: 57  NAASTIRDSREREKKEMSDLNDRLASYIEKVRFLEAQNRKLAADLDALRSKWGKDTHNIR 116

Query: 294 EIYQKEIDQIQSLLKKSETHKEPMKIQIDSFKNKIVEMQKRLVVLEDINSQYRETIEDQR 473
            +Y+ E+   Q L+ ++   ++ M+ Q+   ++++ EM+++L        Q R  I+   
Sbjct: 117 NMYEGELVDAQKLIDETNKQRKDMEGQLKKMQDELAEMRRKLEDATKGREQDRAKIDALL 176

Query: 474 GXXXXXXXXXXXXKKFKAIQDLDSEKTKQETATLYDEITKLRENYEKEVTAQITLEHNIK 653
                        K+  A  + + ++ KQE   L  E+ + R + ++E   +I  ++ ++
Sbjct: 177 VTLSNLEAEISLLKRRIAQLEDEVKRIKQENQRLLSELQRARTDLDQETLNRIDYQNQVQ 236

Query: 654 TFKE 665
           T  E
Sbjct: 237 TLLE 240

 Score = 34.7 bits (78), Expect = 0.29
 Identities = 35/206 (16%), Positives = 82/206 (39%), Gaps = 19/206 (9%)
 Frame = +3

Query: 111 IDNVDSPNGVHSLEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLINEIRKIKGENV 290
           ++ +D    VH  E  ELQ   S +   E    +  ++ +     R   +++  +   ++
Sbjct: 239 LEEIDFLRRVHDNEIKELQTLASRDTTPENREFFKNELSSAIRDIREEYDQVNNVHRNDM 298

Query: 291 QEIYQKEIDQIQSLLKKSETHKEPMKIQIDSFKNKIVEMQKRLVVLEDINSQYRETI--- 461
           +  Y+ ++ +IQ+   +    +   K ++   + ++ +++ +L  LE  NS   + I   
Sbjct: 299 ESWYRLKVQEIQTQSARQNMEQGYAKEEVKRLRTQLSDLRGKLADLESRNSLLEKQIQEL 358

Query: 462 -----EDQRGXXXXXXXXXXXXKKFKAIQD---------LDSEKTKQETATLYDEITKLR 599
                +DQR             +K +             LD+++T      +Y ++ +  
Sbjct: 359 NYQLEDDQRSYEAALNDRDSQIRKMREECQALMVELQMLLDTKQTLDAEIAIYRKMLEGE 418

Query: 600 ENYE--KEVTAQITLEHNIKTFKETK 671
           EN    K++  Q+   H I    +T+
Sbjct: 419 ENRAGLKQLVEQVVKTHAITQETDTE 444
>sp|O02365|IFA2_CAEEL Intermediate filament protein ifa-2 (Intermediate filament protein
           A2) (IF-A2) (Cel IF A2)
          Length = 581

 Score = 62.4 bits (150), Expect = 1e-09
 Identities = 45/189 (23%), Positives = 96/189 (50%), Gaps = 11/189 (5%)
 Frame = +3

Query: 132 NGVHSLEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLINEIRKIKGE------NVQ 293
           N   ++  +  ++KK + E+N+ +A YIEKV+ L+ QNR L  +++ ++G       +V+
Sbjct: 59  NAASTIRDNREREKKEIMELNDRLASYIEKVRFLDAQNRKLDADLKMLQGRFGKSTGSVK 118

Query: 294 EIYQKEIDQIQSLLKKS-----ETHKEPMKIQIDSFKNKIVEMQKRLVVLEDINSQYRET 458
            +Y+ EI    +++K++     ET KE  K+Q     +++ E++K+    +   ++ R  
Sbjct: 119 VMYEMEITTATNVVKQTGKDHGETEKEIRKLQ-----DQLDELRKKFEEAQRGRAEDRLK 173

Query: 459 IEDQRGXXXXXXXXXXXXKKFKAIQDLDSEKTKQETATLYDEITKLRENYEKEVTAQITL 638
           I+D               K+  A+ + +  + K+E   L  E+ ++R   ++E   +I  
Sbjct: 174 IDDLLVTLSNLEAEINLLKRRIALLEEEVARLKKENFRLTSELQRVRIELDQETLLRIDN 233

Query: 639 EHNIKTFKE 665
           ++ +KT  E
Sbjct: 234 QNKVKTILE 242
>sp|P90900|IFA4_CAEEL Intermediate filament protein ifa-4 (Intermediate filament protein
           A4) (IF-A4) (Cel IF A4)
          Length = 575

 Score = 62.0 bits (149), Expect = 2e-09
 Identities = 42/198 (21%), Positives = 93/198 (46%), Gaps = 9/198 (4%)
 Frame = +3

Query: 99  GSNRIDNVDSPNGVHS---LEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLINEIR 269
           G + + +  SP G H+   + +   ++KK + E+N+ +A YIEKV+ LE QNR +  ++ 
Sbjct: 44  GGSNLTSGMSPFGGHAASAIRESRTREKKEMSELNDRLASYIEKVRFLEAQNRKMEKDLN 103

Query: 270 KIKGE------NVQEIYQKEIDQIQSLLKKSETHKEPMKIQIDSFKNKIVEMQKRLVVLE 431
            ++G+      +V+ +Y+ E+   + L+  S+  +  ++ QI     ++   + +L   E
Sbjct: 104 LLRGKWGHDSTSVKVMYETELRSAKDLIADSDKERAQLEDQIKKLVEELNNYRNKLHEAE 163

Query: 432 DINSQYRETIEDQRGXXXXXXXXXXXXKKFKAIQDLDSEKTKQETATLYDEITKLRENYE 611
                 R+ +++  G            K+  ++ +      K+E   + D +   R   E
Sbjct: 164 RGADVTRKELDNVLGRLGALEAEIELLKRRISLMEESIGHLKRENHRMIDSLHHARNAVE 223

Query: 612 KEVTAQITLEHNIKTFKE 665
           +E   +I  ++ ++T  E
Sbjct: 224 QETLNRIDYQNQVQTLLE 241
>sp|Q03427|LAMC_DROME Lamin C (pG-IF)
          Length = 621

 Score = 58.2 bits (139), Expect = 2e-08
 Identities = 48/215 (22%), Positives = 96/215 (44%), Gaps = 26/215 (12%)
 Frame = +3

Query: 99  GSNRIDNVDSPNGVHSLEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLINEIRKIK 278
           G++    V + +          Q+K+ L+ +N+ +A YI++++NLE +N  L  E+   +
Sbjct: 23  GASTSSRVGATSPTSPTRTSRQQEKEELQHLNDRLACYIDRMRNLENENSRLTQELNLAQ 82

Query: 279 G------ENVQEIYQKEIDQIQSLLKKSETHKEPMKIQI---------DSFKNKIVEMQK 413
                   N++ +Y+KE+   + LL   ET KE  K++I         D  K ++ +  K
Sbjct: 83  DTVNRETSNLKAVYEKELAAARKLL--DETAKEKAKLEIDIKRLWEENDDLKPRLDKKTK 140

Query: 414 RLVVLE-----------DINSQYRETIEDQRGXXXXXXXXXXXXKKFKAIQDLDSEKTKQ 560
              V E           ++N +Y +++ D+              KKF+      +++   
Sbjct: 141 EATVAENNARLYENRYNEVNGKYNQSLADR--------------KKFED----QAKELAL 182

Query: 561 ETATLYDEITKLRENYEKEVTAQITLEHNIKTFKE 665
           E   L  ++  LR+  E E  A++ LE+  ++ +E
Sbjct: 183 ENERLRRQLDDLRKQLEAETLARVDLENQNQSLRE 217
>sp|Q19289|IFB1_CAEEL Intermediate filament protein ifb-1 (Intermediate filament protein
           B1) (IF-B1) (Cel IF B1)
          Length = 589

 Score = 57.8 bits (138), Expect = 3e-08
 Identities = 40/190 (21%), Positives = 89/190 (46%), Gaps = 7/190 (3%)
 Frame = +3

Query: 126 SPNGVHSLEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLI---NEIRKIKGENVQE 296
           S N   S  +   ++KK+L+ +N+ +  YI++VK LE QNR L+   +E+R   G++  E
Sbjct: 67  SANAAKSFLEATDKEKKTLQGLNDRLGNYIDRVKKLEEQNRKLVADLDELRGKWGKDTSE 126

Query: 297 I---YQKEIDQIQSLLKKSETHKEPMKIQIDSFKNKIVEMQKRLVVLEDINSQYRETIED 467
           I   Y + +   +  +  +   K  + +++   ++ + E + R   ++      RE I  
Sbjct: 127 IKIKYSESLSTARKDIDDAARRKAEVDVKVARHRDDLAEYRSRYEDIQQRRESDREKISQ 186

Query: 468 QRGXXXXXXXXXXXXK-KFKAIQDLDSEKTKQETATLYDEITKLRENYEKEVTAQITLEH 644
                          + +FK + D + ++   + + +++E+ K R + + E   +I  ++
Sbjct: 187 WTNAIADAQSEVEMLRARFKQLTD-EEKRVTADNSRIWEELQKARSDLDDETIGRIDFQN 245

Query: 645 NIKTFKETKE 674
            ++T  E  E
Sbjct: 246 QVQTLMEELE 255
>sp|Q21065|IFA3_CAEEL Intermediate filament protein ifa-3 (Intermediate filament protein
           A3) (IF-A3) (Cel IF A3)
          Length = 581

 Score = 56.6 bits (135), Expect = 7e-08
 Identities = 38/180 (21%), Positives = 88/180 (48%), Gaps = 6/180 (3%)
 Frame = +3

Query: 144 SLEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLINEIRKIKGE------NVQEIYQ 305
           ++  D  ++KK + E+N+ +A YI KV+ L  QNR L  ++  ++        +V+ +Y+
Sbjct: 63  TIRDDREREKKEITELNDRLASYIGKVRFLAAQNRKLEADLNVLQSRFGKSTGSVKIMYE 122

Query: 306 KEIDQIQSLLKKSETHKEPMKIQIDSFKNKIVEMQKRLVVLEDINSQYRETIEDQRGXXX 485
            EI    +++K++    E  + +I   K+++ E++K+    +   ++ R  I++      
Sbjct: 123 MEITTATNVVKETGKDHEEAEKEIGKIKDQLDELRKKFEEAQKGRAEDRLKIDELLVTLS 182

Query: 486 XXXXXXXXXKKFKAIQDLDSEKTKQETATLYDEITKLRENYEKEVTAQITLEHNIKTFKE 665
                    K+  A+ + +  + K+E   L  E+ ++R   ++E   +I  ++ + T  E
Sbjct: 183 NLEAEINLLKRRIALLEEEVARLKKENFRLTSELQRVRSELDQETLLRIDNQNKVTTILE 242
>sp|P23731|IFEB_ASCSU Intermediate filament protein B (IF-B)
          Length = 589

 Score = 55.8 bits (133), Expect = 1e-07
 Identities = 39/190 (20%), Positives = 91/190 (47%), Gaps = 7/190 (3%)
 Frame = +3

Query: 126 SPNGVHSLEKDELQKKKSLEEINEGIAIYIEKVKNLEIQNRNLI---NEIRKIKGENVQE 296
           S N   S  +   ++KK ++ +N+ +  YI++VK LE QNR L+   +E+R   G++  E
Sbjct: 67  SANAAKSFLEATDKEKKEMQGLNDRLGNYIDRVKKLEEQNRKLVADLDELRGRWGKDTSE 126

Query: 297 IYQKEIDQIQSLLKKSE---THKEPMKIQIDSFKNKIVEMQKRLVVLEDINSQYRETIED 467
           I  +  D ++   K+ +     K  + +++   ++ + E++ R   ++      RE I  
Sbjct: 127 IKIQYSDSLRDARKEIDDGARRKAEIDVKVARLRDDLAELRNRYEDVQHRRESDREKINQ 186

Query: 468 -QRGXXXXXXXXXXXXKKFKAIQDLDSEKTKQETATLYDEITKLRENYEKEVTAQITLEH 644
            Q               +++ + + + ++   + A +++E+ K R + ++E   +I  ++
Sbjct: 187 WQHAIEDAQSELEMLRARWRQLTE-EEKRLNGDNARIWEELQKARNDLDEETLGRIDFQN 245

Query: 645 NIKTFKETKE 674
            ++T  E  E
Sbjct: 246 QVQTLMEELE 255
>sp|P35617|XNIF_XENLA Low molecular weight neuronal intermediate filament (XNIF)
          Length = 470

 Score = 54.3 bits (129), Expect = 3e-07
 Identities = 42/181 (23%), Positives = 88/181 (48%), Gaps = 20/181 (11%)
 Frame = +3

Query: 168 KKKSLEEINEGIAIYIEKVKNLEIQNRNLINEIRKIKGEN-----VQEIYQKEIDQIQSL 332
           +K+ L+ +N+    YIEKV +LE QN+ L +E+  ++ ++     +  IY++EI +++S 
Sbjct: 88  EKEQLQGLNDRFVTYIEKVHHLEQQNKLLESEVTLLRQKHSEPSRLSHIYEQEIRELRSK 147

Query: 333 LKKSETHKEPMKIQ-------IDSFKNKIVEMQKRLVVLEDINSQYRETIEDQRGXXXXX 491
           L++ E  K+  ++        ++  K K+ +   R    ED+   YR+ ++         
Sbjct: 148 LEEQEQDKDQAQLDYEHLGACLEQLKLKLEQESARREEAEDVMKNYRKDLDQ-------- 199

Query: 492 XXXXXXXKKFKAIQDLDSEKTKQETATLYDEITKLRENYEKEVT--------AQITLEHN 647
                          L+  + +++  +L DEI  LR+ +E+E+         AQI++E +
Sbjct: 200 -------------ATLNRLQLEKKVESLLDEIAFLRKVHEEEIAELQASVQEAQISVEMD 246

Query: 648 I 650
           +
Sbjct: 247 V 247
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,446,858
Number of Sequences: 369166
Number of extensions: 913823
Number of successful extensions: 4719
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4226
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4643
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6970118400
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)