Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_02518
(513 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O13830|PTH2_SCHPO Probable peptidyl-tRNA hydrolase 2 (PT... 159 3e-39
sp|Q8R2Y8|PTH2_MOUSE Peptidyl-tRNA hydrolase 2, mitochondri... 147 2e-35
sp|Q9Y3E5|PTH2_HUMAN Peptidyl-tRNA hydrolase 2, mitochondri... 143 2e-34
sp|P34222|PTH2_YEAST Peptidyl-tRNA hydrolase 2 (PTH 2) 124 1e-28
sp|O97067|PTH2_DROME Probable peptidyl-tRNA hydrolase 2 (PT... 110 3e-24
sp|Q5JDB8|PTH_PYRKO Peptidyl-tRNA hydrolase (PTH) 107 2e-23
sp|Q8U0N0|PTH_PYRFU Peptidyl-tRNA hydrolase (PTH) 106 3e-23
sp|O74017|PTH_PYRHO Peptidyl-tRNA hydrolase (PTH) 106 4e-23
sp|Q976I0|PTH_SULTO Peptidyl-tRNA hydrolase (PTH) 105 6e-23
sp|Q9HLW6|PTH_THEAC Peptidyl-tRNA hydrolase (PTH) 101 9e-22
>sp|O13830|PTH2_SCHPO Probable peptidyl-tRNA hydrolase 2 (PTH 2)
Length = 205
Score = 159 bits (403), Expect = 3e-39
Identities = 76/116 (65%), Positives = 97/116 (83%)
Frame = +3
Query: 147 ESKMVLVVRTDLGMGKGKVAAQCAHAALGCYKSGLKKAPQIVKAWELSGQAKVVVKTENE 326
++KMVLVVRTDLGM KGK+AAQCAHAAL CYK P +V+ WE +GQAK+ ++ + E
Sbjct: 90 QTKMVLVVRTDLGMTKGKIAAQCAHAALACYKIASAVDPDLVRIWENAGQAKITLQAQTE 149
Query: 327 ESLYELQAMAISLGLISKVVHDAGRTQIAAGSATVVGIGPGPVSLVNKVTGQLKLY 494
E+L LQA A+SLGL ++V+HDAGRTQIA+GSATV+GIGPGPVS++N+VTG LKL+
Sbjct: 150 ETLELLQAQAMSLGLCARVIHDAGRTQIASGSATVLGIGPGPVSVINEVTGSLKLF 205
>sp|Q8R2Y8|PTH2_MOUSE Peptidyl-tRNA hydrolase 2, mitochondrial precursor (PTH 2)
Length = 181
Score = 147 bits (370), Expect = 2e-35
Identities = 80/168 (47%), Positives = 105/168 (62%), Gaps = 15/168 (8%)
Frame = +3
Query: 36 SAGLGVLLGIIVTWKFKPKPFRNVELMPQEMTSHT---------------SEESKMVLVV 170
S GV G+ + W + ++ + PQ TS S E KM+LVV
Sbjct: 18 SLAAGVACGMCLGWGLRS----HLGMFPQNSTSEANRDTETGTEASILGESGEYKMILVV 73
Query: 171 RTDLGMGKGKVAAQCAHAALGCYKSGLKKAPQIVKAWELSGQAKVVVKTENEESLYELQA 350
RTDL MGKGKVAAQC+HAA+ YK +++PQ++K WE GQ KVVVK +E++L +L
Sbjct: 74 RTDLKMGKGKVAAQCSHAAVSAYKQTQRRSPQVLKEWEYCGQPKVVVKAPDEDTLIQLLT 133
Query: 351 MAISLGLISKVVHDAGRTQIAAGSATVVGIGPGPVSLVNKVTGQLKLY 494
A +LGL ++ DAGRTQI GS TV+GIGPGPV L+++VTG LKLY
Sbjct: 134 HAKTLGLTVSLIQDAGRTQIEPGSRTVLGIGPGPVELIDEVTGHLKLY 181
>sp|Q9Y3E5|PTH2_HUMAN Peptidyl-tRNA hydrolase 2, mitochondrial precursor (PTH 2) (Bcl-2
inhibitor of transcription 1)
Length = 179
Score = 143 bits (361), Expect = 2e-34
Identities = 81/163 (49%), Positives = 103/163 (63%), Gaps = 13/163 (7%)
Frame = +3
Query: 45 LGVLLGIIVTWKFKPKPFRNVELMPQEMTS--HTSEES-----------KMVLVVRTDLG 185
+GV G+ + W + ++P+ TS HT ES KM+LVVR DL
Sbjct: 21 VGVACGMCLGWSLRVC----FGMLPKSKTSKTHTDTESEASILGDSGEYKMILVVRNDLK 76
Query: 186 MGKGKVAAQCAHAALGCYKSGLKKAPQIVKAWELSGQAKVVVKTENEESLYELQAMAISL 365
MGKGKVAAQC+HAA+ YK ++ P+++K WE GQ KVVVK +EE+L L A A L
Sbjct: 77 MGKGKVAAQCSHAAVSAYKQIQRRNPEMLKQWEYCGQPKVVVKAPDEETLIALLAHAKML 136
Query: 366 GLISKVVHDAGRTQIAAGSATVVGIGPGPVSLVNKVTGQLKLY 494
GL ++ DAGRTQIA GS TV+GIGPGP L++KVTG LKLY
Sbjct: 137 GLTVSLIQDAGRTQIAPGSQTVLGIGPGPADLIDKVTGHLKLY 179
>sp|P34222|PTH2_YEAST Peptidyl-tRNA hydrolase 2 (PTH 2)
Length = 214
Score = 124 bits (311), Expect = 1e-28
Identities = 62/122 (50%), Positives = 88/122 (72%), Gaps = 6/122 (4%)
Frame = +3
Query: 147 ESKMVLVVRTDLGMGKGKVAAQCAHAALGCYK------SGLKKAPQIVKAWELSGQAKVV 308
E +M LV+R DLGM KGK+AAQC HAAL C++ + P + + W +GQAK+
Sbjct: 93 EVRMALVIRQDLGMTKGKIAAQCCHAALSCFRHIATNPARASYNPIMTQRWLNAGQAKIT 152
Query: 309 VKTENEESLYELQAMAISLGLISKVVHDAGRTQIAAGSATVVGIGPGPVSLVNKVTGQLK 488
+K ++ ++ EL A AISLG+ + V+HDAGRTQIAAGSATV+G+GP P ++++++TG LK
Sbjct: 153 LKCPDKFTMDELYAKAISLGVNAAVIHDAGRTQIAAGSATVLGLGPAPKAVLDQITGDLK 212
Query: 489 LY 494
LY
Sbjct: 213 LY 214
>sp|O97067|PTH2_DROME Probable peptidyl-tRNA hydrolase 2 (PTH 2)
Length = 186
Score = 110 bits (274), Expect = 3e-24
Identities = 53/117 (45%), Positives = 79/117 (67%)
Frame = +3
Query: 141 SEESKMVLVVRTDLGMGKGKVAAQCAHAALGCYKSGLKKAPQIVKAWELSGQAKVVVKTE 320
++ KMVLVVR DL MGKGK+AAQC H A+G Y+ + + P+++++WE G AK+ V+ E
Sbjct: 69 NDNFKMVLVVRNDLKMGKGKIAAQCGHGAVGAYQRAVVRTPRLLRSWENCGCAKIAVRVE 128
Query: 321 NEESLYELQAMAISLGLISKVVHDAGRTQIAAGSATVVGIGPGPVSLVNKVTGQLKL 491
+E L ++ A L + ++ DAGRTQI A S TV+ +GP + +++VTG LKL
Sbjct: 129 SEAELMAIKKEAERQQLNTCLIRDAGRTQIEANSKTVLAVGPAAAADIDRVTGHLKL 185
>sp|Q5JDB8|PTH_PYRKO Peptidyl-tRNA hydrolase (PTH)
Length = 118
Score = 107 bits (266), Expect = 2e-23
Identities = 57/113 (50%), Positives = 73/113 (64%)
Frame = +3
Query: 153 KMVLVVRTDLGMGKGKVAAQCAHAALGCYKSGLKKAPQIVKAWELSGQAKVVVKTENEES 332
K V+V R DL + KGK A Q AH A+ K+ P+ KAW GQ KVVVK EN E
Sbjct: 5 KQVIVARKDLKLSKGKFAVQVAHGAVTAAIKAQKEKPEWFKAWFHEGQKKVVVKAENLEE 64
Query: 333 LYELQAMAISLGLISKVVHDAGRTQIAAGSATVVGIGPGPVSLVNKVTGQLKL 491
L++L+A A LGL + ++ DAG T+I G+ TV+ IGPGP +V+KVTG LKL
Sbjct: 65 LFKLKAEAEKLGLPTALIKDAGLTEIPPGTVTVLAIGPGPEEVVDKVTGHLKL 117
>sp|Q8U0N0|PTH_PYRFU Peptidyl-tRNA hydrolase (PTH)
Length = 119
Score = 106 bits (265), Expect = 3e-23
Identities = 55/113 (48%), Positives = 75/113 (66%)
Frame = +3
Query: 153 KMVLVVRTDLGMGKGKVAAQCAHAALGCYKSGLKKAPQIVKAWELSGQAKVVVKTENEES 332
K V+V R+DL + KGK+A Q AH A+ KK + +AW GQ KVVVK +NEE
Sbjct: 6 KQVIVARSDLKLSKGKLAVQVAHGAVTAAFEAYKKKREWFEAWFREGQKKVVVKAQNEEE 65
Query: 333 LYELQAMAISLGLISKVVHDAGRTQIAAGSATVVGIGPGPVSLVNKVTGQLKL 491
L++L++ A LGL + ++ DAG T+I G+ TV+ IGPGP +V+KVTG LKL
Sbjct: 66 LFKLKSEAEKLGLPTALIRDAGLTEIPPGTITVLAIGPGPEEVVDKVTGHLKL 118
>sp|O74017|PTH_PYRHO Peptidyl-tRNA hydrolase (PTH)
Length = 121
Score = 106 bits (264), Expect = 4e-23
Identities = 55/113 (48%), Positives = 74/113 (65%)
Frame = +3
Query: 153 KMVLVVRTDLGMGKGKVAAQCAHAALGCYKSGLKKAPQIVKAWELSGQAKVVVKTENEES 332
K V+V R DL + KGK+AAQ AH A+ KK + +AW GQ KVVVK E+EE
Sbjct: 8 KQVIVARADLKLSKGKLAAQVAHGAVTAAFEAYKKKREWFEAWFREGQKKVVVKVESEEE 67
Query: 333 LYELQAMAISLGLISKVVHDAGRTQIAAGSATVVGIGPGPVSLVNKVTGQLKL 491
L++L+A A LGL + ++ DAG T+I G+ TV+ +GP P +V+KVTG LKL
Sbjct: 68 LFKLKAEAEKLGLPNALIRDAGLTEIPPGTVTVLAVGPAPEEIVDKVTGNLKL 120
>sp|Q976I0|PTH_SULTO Peptidyl-tRNA hydrolase (PTH)
Length = 121
Score = 105 bits (262), Expect = 6e-23
Identities = 57/118 (48%), Positives = 75/118 (63%), Gaps = 4/118 (3%)
Frame = +3
Query: 150 SKMVLVVRTDLGMGKGKVAAQCAHAALGCYKSGLKKAPQIVKAWELS----GQAKVVVKT 317
+KMV+VVRTDL MGKGK+AAQ AHAA+ L + + K W GQ K+VVK
Sbjct: 3 TKMVIVVRTDLDMGKGKIAAQVAHAAVSLVLDILNSSNSLWKEWLYKWLEEGQPKIVVKV 62
Query: 318 ENEESLYELQAMAISLGLISKVVHDAGRTQIAAGSATVVGIGPGPVSLVNKVTGQLKL 491
+ + L + AI L L + ++ DAG+TQI G+ T +GIGP PV LV+K+TG LKL
Sbjct: 63 PSLDELNKRYEKAIELNLPTTIIQDAGKTQIEPGTITCIGIGPAPVELVDKITGDLKL 120
>sp|Q9HLW6|PTH_THEAC Peptidyl-tRNA hydrolase (PTH)
Length = 117
Score = 101 bits (252), Expect = 9e-22
Identities = 48/113 (42%), Positives = 73/113 (64%)
Frame = +3
Query: 153 KMVLVVRTDLGMGKGKVAAQCAHAALGCYKSGLKKAPQIVKAWELSGQAKVVVKTENEES 332
KMV+ VR DL MGKGK+AAQ AHAA+ C +K + W GQ K+VVK + +
Sbjct: 4 KMVIAVRKDLDMGKGKIAAQVAHAAVTCAIRSMKINRDVFNEWYDEGQRKIVVKVNDLDE 63
Query: 333 LYELQAMAISLGLISKVVHDAGRTQIAAGSATVVGIGPGPVSLVNKVTGQLKL 491
+ E++ MA S+G+++++V D G TQ+ G+ T +G+GP ++K+TG+ KL
Sbjct: 64 IMEIKRMADSMGIVNEIVQDRGYTQVEPGTITCIGLGPDEEEKLDKITGKYKL 116
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 61,362,492
Number of Sequences: 369166
Number of extensions: 1167561
Number of successful extensions: 3490
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3377
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3472
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3304927425
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)