Planaria EST Database


DrC_02416

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_02416
         (535 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9CWZ3|RBM8A_MOUSE  RNA-binding protein 8A (RNA-binding m...   177   1e-44
sp|Q9V535|RBM8A_DROME  RNA-binding protein 8A (Tsunagi protein)   157   2e-38
sp|Q8C7E9|CSTFT_MOUSE  Cleavage stimulation factor, 64 kDa s...    63   4e-10
sp|Q9H0L4|CSTFT_HUMAN  Cleavage stimulation factor, 64 kDa s...    63   4e-10
sp|Q8BIQ5|CSTF2_MOUSE  Cleavage stimulation factor, 64 kDa s...    63   5e-10
sp|P33240|CSTF2_HUMAN  Cleavage stimulation factor, 64 kDa s...    63   5e-10
sp|Q5RDA3|CSTF2_PONPY  Cleavage stimulation factor, 64 kDa s...    63   5e-10
sp|Q8HXM1|CSTF2_BOVIN  Cleavage stimulation factor, 64 kDa s...    63   5e-10
sp|P52912|TIA1_MOUSE  Nucleolysin TIA-1 (RNA-binding protein...    61   1e-09
sp|P31483|TIA1_HUMAN  Nucleolysin TIA-1 isoform p40 (RNA-bin...    59   6e-09
>sp|Q9CWZ3|RBM8A_MOUSE RNA-binding protein 8A (RNA-binding motif protein 8A)
           (Ribonucleoprotein RBM8A)
 sp|Q9Y5S9|RBM8A_HUMAN RNA-binding protein 8A (RNA-binding motif protein 8A)
           (Ribonucleoprotein RBM8A) (RNA-binding protein Y14)
           (Binder of OVCA1-1) (BOV-1)
          Length = 174

 Score =  177 bits (450), Expect = 1e-44
 Identities = 79/138 (57%), Positives = 106/138 (76%)
 Frame = +2

Query: 11  MDVDGDEKIIELKENVKSRKGRGFDNEQRHDRNTKFEALSDTEDISGPGPQRSVEGWIIF 190
           MD DGDE I +LKE  K RKGRGF +E+      + +  S  +D   PGPQRSVEGWI+F
Sbjct: 17  MDEDGDESIHKLKEKAKKRKGRGFGSEEGSRARMREDYDSVEQDGDEPGPQRSVEGWILF 76

Query: 191 IRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIEYETFAEAQNALKTMSG 370
           +  +HEEATEED+ DKF+E+GEIKN+H+N DRRTGY+KGY L+EYET+ EAQ A++ ++G
Sbjct: 77  VTGVHEEATEEDIHDKFAEYGEIKNIHLNLDRRTGYLKGYTLVEYETYKEAQAAMEGLNG 136

Query: 371 EEMYGEKINVDWAFVKGP 424
           +++ G+ I+VDW FV+GP
Sbjct: 137 QDLMGQPISVDWCFVRGP 154
>sp|Q9V535|RBM8A_DROME RNA-binding protein 8A (Tsunagi protein)
          Length = 165

 Score =  157 bits (397), Expect = 2e-38
 Identities = 72/140 (51%), Positives = 101/140 (72%), Gaps = 2/140 (1%)
 Frame = +2

Query: 11  MDVDGDEKIIELKENVKSRKGRGF--DNEQRHDRNTKFEALSDTEDISGPGPQRSVEGWI 184
           +D DGD+ I+ LKE  K RKGRGF  D+  R   ++     ++ +D   PGPQRSVEGWI
Sbjct: 15  VDEDGDQGIVRLKEKAKHRKGRGFGSDSNTREAIHSYERVRNEDDDELEPGPQRSVEGWI 74

Query: 185 IFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIEYETFAEAQNALKTM 364
           +F+ +IHEEA E+++++KF ++GEIKN+H+N DRRTG+ KGY L+EYET  +A  A + +
Sbjct: 75  LFVTSIHEEAQEDEIQEKFCDYGEIKNIHLNLDRRTGFSKGYALVEYETHKQALAAKEAL 134

Query: 365 SGEEMYGEKINVDWAFVKGP 424
           +G E+ G+ I VDW FVKGP
Sbjct: 135 NGAEIMGQTIQVDWCFVKGP 154
>sp|Q8C7E9|CSTFT_MOUSE Cleavage stimulation factor, 64 kDa subunit, tau variant (CSTF 64
           kDa subunit, tau variant) (CF-1 64 kDa subunit, tau
           variant) (TauCstF-64)
          Length = 632

 Score = 63.2 bits (152), Expect = 4e-10
 Identities = 32/87 (36%), Positives = 49/87 (56%)
 Frame = +2

Query: 143 ISGPGPQRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIE 322
           +  P   RS+    +F+ NI  EATEE ++D FSE G + +  + +DR TG  KGY   E
Sbjct: 6   VRDPAMDRSLRS--VFVGNIPYEATEEQLKDIFSEVGSVVSFRLVYDRETGKPKGYGFCE 63

Query: 323 YETFAEAQNALKTMSGEEMYGEKINVD 403
           Y+    A +A++ ++G E  G  + VD
Sbjct: 64  YQDQETALSAMRNLNGREFSGRALRVD 90
>sp|Q9H0L4|CSTFT_HUMAN Cleavage stimulation factor, 64 kDa subunit, tau variant (CSTF 64
           kDa subunit, tau variant) (CF-1 64 kDa subunit, tau
           variant) (TauCstF-64)
          Length = 616

 Score = 63.2 bits (152), Expect = 4e-10
 Identities = 32/87 (36%), Positives = 49/87 (56%)
 Frame = +2

Query: 143 ISGPGPQRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIE 322
           +  P   RS+    +F+ NI  EATEE ++D FSE G + +  + +DR TG  KGY   E
Sbjct: 6   VRDPAMDRSLRS--VFVGNIPYEATEEQLKDIFSEVGSVVSFRLVYDRETGKPKGYGFCE 63

Query: 323 YETFAEAQNALKTMSGEEMYGEKINVD 403
           Y+    A +A++ ++G E  G  + VD
Sbjct: 64  YQDQETALSAMRNLNGREFSGRALRVD 90
>sp|Q8BIQ5|CSTF2_MOUSE Cleavage stimulation factor, 64 kDa subunit (CSTF 64 kDa subunit)
           (CF-1 64 kDa subunit) (CstF-64)
          Length = 580

 Score = 62.8 bits (151), Expect = 5e-10
 Identities = 32/87 (36%), Positives = 49/87 (56%)
 Frame = +2

Query: 143 ISGPGPQRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIE 322
           +  P   RS+    +F+ NI  EATEE ++D FSE G + +  + +DR TG  KGY   E
Sbjct: 6   VRDPAVDRSLRS--VFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCE 63

Query: 323 YETFAEAQNALKTMSGEEMYGEKINVD 403
           Y+    A +A++ ++G E  G  + VD
Sbjct: 64  YQDQETALSAMRNLNGREFSGRALRVD 90
>sp|P33240|CSTF2_HUMAN Cleavage stimulation factor, 64 kDa subunit (CSTF 64 kDa subunit)
           (CF-1 64 kDa subunit) (CstF-64)
          Length = 577

 Score = 62.8 bits (151), Expect = 5e-10
 Identities = 32/87 (36%), Positives = 49/87 (56%)
 Frame = +2

Query: 143 ISGPGPQRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIE 322
           +  P   RS+    +F+ NI  EATEE ++D FSE G + +  + +DR TG  KGY   E
Sbjct: 6   VRDPAVDRSLRS--VFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCE 63

Query: 323 YETFAEAQNALKTMSGEEMYGEKINVD 403
           Y+    A +A++ ++G E  G  + VD
Sbjct: 64  YQDQETALSAMRNLNGREFSGRALRVD 90
>sp|Q5RDA3|CSTF2_PONPY Cleavage stimulation factor, 64 kDa subunit (CSTF 64 kDa subunit)
           (CF-1 64 kDa subunit) (CstF-64)
          Length = 577

 Score = 62.8 bits (151), Expect = 5e-10
 Identities = 32/87 (36%), Positives = 49/87 (56%)
 Frame = +2

Query: 143 ISGPGPQRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIE 322
           +  P   RS+    +F+ NI  EATEE ++D FSE G + +  + +DR TG  KGY   E
Sbjct: 6   VRDPAVDRSLRS--VFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCE 63

Query: 323 YETFAEAQNALKTMSGEEMYGEKINVD 403
           Y+    A +A++ ++G E  G  + VD
Sbjct: 64  YQDQETALSAMRNLNGREFSGRALRVD 90
>sp|Q8HXM1|CSTF2_BOVIN Cleavage stimulation factor, 64 kDa subunit (CSTF 64 kDa subunit)
           (CF-1 64 kDa subunit) (CstF-64)
          Length = 572

 Score = 62.8 bits (151), Expect = 5e-10
 Identities = 32/87 (36%), Positives = 49/87 (56%)
 Frame = +2

Query: 143 ISGPGPQRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIE 322
           +  P   RS+    +F+ NI  EATEE ++D FSE G + +  + +DR TG  KGY   E
Sbjct: 6   VRDPAVDRSLRS--VFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCE 63

Query: 323 YETFAEAQNALKTMSGEEMYGEKINVD 403
           Y+    A +A++ ++G E  G  + VD
Sbjct: 64  YQDQETALSAMRNLNGREFSGRALRVD 90
>sp|P52912|TIA1_MOUSE Nucleolysin TIA-1 (RNA-binding protein TIA-1)
          Length = 386

 Score = 61.2 bits (147), Expect = 1e-09
 Identities = 31/98 (31%), Positives = 53/98 (54%)
 Frame = +2

Query: 131 DTEDISGPGPQRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGY 310
           DT   +    QRS + + +F+ ++  E T ED++  F+ FG I +  +  D  TG  KGY
Sbjct: 90  DTSSSTVVSTQRSQDHFHVFVGDLSPEITTEDIKAAFAPFGRISDARVVKDMATGKSKGY 149

Query: 311 VLIEYETFAEAQNALKTMSGEEMYGEKINVDWAFVKGP 424
             + +    +A+NA++ M G+ + G +I  +WA  K P
Sbjct: 150 GFVSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPP 187

 Score = 43.5 bits (101), Expect = 3e-04
 Identities = 22/75 (29%), Positives = 38/75 (50%)
 Frame = +2

Query: 185 IFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIEYETFAEAQNALKTM 364
           +++ N+  + TE  +   FS+ G  KN  M  D  T     Y  +E+     A  AL  M
Sbjct: 9   LYVGNLSRDVTEALILQLFSQIGPCKNCKMIMD--TAGNDPYCFVEFHEHRHAAAALAAM 66

Query: 365 SGEEMYGEKINVDWA 409
           +G ++ G+++ V+WA
Sbjct: 67  NGRKIMGKEVKVNWA 81

 Score = 35.4 bits (80), Expect = 0.088
 Identities = 25/103 (24%), Positives = 46/103 (44%)
 Frame = +2

Query: 98  HDRNTKFEALSDTEDISGPGPQRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMN 277
           ++ NTK   LS  E +S   P        ++   +    TE+ +R  FS FG+I  + + 
Sbjct: 193 YESNTK--QLSYDEVVSQSSPNNCT----VYCGGVTSGLTEQLMRQTFSPFGQIMEIRVF 246

Query: 278 FDRRTGYIKGYVLIEYETFAEAQNALKTMSGEEMYGEKINVDW 406
            D      KGY  + + +   A +A+ +++G  + G  +   W
Sbjct: 247 PD------KGYSFVRFSSHESAAHAIVSVNGTTIEGHVVKCYW 283
>sp|P31483|TIA1_HUMAN Nucleolysin TIA-1 isoform p40 (RNA-binding protein TIA-1)
           (p40-TIA-1) [Contains: Nucleolysin TIA-1 isoform p15
           (p15-TIA-1)]
          Length = 386

 Score = 59.3 bits (142), Expect = 6e-09
 Identities = 30/98 (30%), Positives = 52/98 (53%)
 Frame = +2

Query: 131 DTEDISGPGPQRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGY 310
           DT   +    QRS   + +F+ ++  + T ED++  F+ FG I +  +  D  TG  KGY
Sbjct: 90  DTSSSTVVSTQRSQNHFHVFVGDLSPQITTEDIKAAFAPFGRISDARVVKDMATGKSKGY 149

Query: 311 VLIEYETFAEAQNALKTMSGEEMYGEKINVDWAFVKGP 424
             + +    +A+NA++ M G+ + G +I  +WA  K P
Sbjct: 150 GFVSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPP 187

 Score = 43.5 bits (101), Expect = 3e-04
 Identities = 22/75 (29%), Positives = 38/75 (50%)
 Frame = +2

Query: 185 IFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIEYETFAEAQNALKTM 364
           +++ N+  + TE  +   FS+ G  KN  M  D  T     Y  +E+     A  AL  M
Sbjct: 9   LYVGNLSRDVTEALILQLFSQIGPCKNCKMIMD--TAGNDPYCFVEFHEHRHAAAALAAM 66

Query: 365 SGEEMYGEKINVDWA 409
           +G ++ G+++ V+WA
Sbjct: 67  NGRKIMGKEVKVNWA 81

 Score = 34.3 bits (77), Expect = 0.20
 Identities = 19/82 (23%), Positives = 37/82 (45%)
 Frame = +2

Query: 161 QRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIEYETFAE 340
           Q S     ++   +    TE+ +R  FS FG+I  + +  D      KGY  + + +   
Sbjct: 208 QSSPSNCTVYCGGVTSGLTEQLMRQTFSPFGQIMEIRVFPD------KGYSFVRFNSHES 261

Query: 341 AQNALKTMSGEEMYGEKINVDW 406
           A +A+ +++G  + G  +   W
Sbjct: 262 AAHAIVSVNGTTIEGHVVKCYW 283
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49,616,516
Number of Sequences: 369166
Number of extensions: 860126
Number of successful extensions: 2560
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2372
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2513
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3650218350
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)