Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_02416
(535 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9CWZ3|RBM8A_MOUSE RNA-binding protein 8A (RNA-binding m... 177 1e-44
sp|Q9V535|RBM8A_DROME RNA-binding protein 8A (Tsunagi protein) 157 2e-38
sp|Q8C7E9|CSTFT_MOUSE Cleavage stimulation factor, 64 kDa s... 63 4e-10
sp|Q9H0L4|CSTFT_HUMAN Cleavage stimulation factor, 64 kDa s... 63 4e-10
sp|Q8BIQ5|CSTF2_MOUSE Cleavage stimulation factor, 64 kDa s... 63 5e-10
sp|P33240|CSTF2_HUMAN Cleavage stimulation factor, 64 kDa s... 63 5e-10
sp|Q5RDA3|CSTF2_PONPY Cleavage stimulation factor, 64 kDa s... 63 5e-10
sp|Q8HXM1|CSTF2_BOVIN Cleavage stimulation factor, 64 kDa s... 63 5e-10
sp|P52912|TIA1_MOUSE Nucleolysin TIA-1 (RNA-binding protein... 61 1e-09
sp|P31483|TIA1_HUMAN Nucleolysin TIA-1 isoform p40 (RNA-bin... 59 6e-09
>sp|Q9CWZ3|RBM8A_MOUSE RNA-binding protein 8A (RNA-binding motif protein 8A)
(Ribonucleoprotein RBM8A)
sp|Q9Y5S9|RBM8A_HUMAN RNA-binding protein 8A (RNA-binding motif protein 8A)
(Ribonucleoprotein RBM8A) (RNA-binding protein Y14)
(Binder of OVCA1-1) (BOV-1)
Length = 174
Score = 177 bits (450), Expect = 1e-44
Identities = 79/138 (57%), Positives = 106/138 (76%)
Frame = +2
Query: 11 MDVDGDEKIIELKENVKSRKGRGFDNEQRHDRNTKFEALSDTEDISGPGPQRSVEGWIIF 190
MD DGDE I +LKE K RKGRGF +E+ + + S +D PGPQRSVEGWI+F
Sbjct: 17 MDEDGDESIHKLKEKAKKRKGRGFGSEEGSRARMREDYDSVEQDGDEPGPQRSVEGWILF 76
Query: 191 IRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIEYETFAEAQNALKTMSG 370
+ +HEEATEED+ DKF+E+GEIKN+H+N DRRTGY+KGY L+EYET+ EAQ A++ ++G
Sbjct: 77 VTGVHEEATEEDIHDKFAEYGEIKNIHLNLDRRTGYLKGYTLVEYETYKEAQAAMEGLNG 136
Query: 371 EEMYGEKINVDWAFVKGP 424
+++ G+ I+VDW FV+GP
Sbjct: 137 QDLMGQPISVDWCFVRGP 154
>sp|Q9V535|RBM8A_DROME RNA-binding protein 8A (Tsunagi protein)
Length = 165
Score = 157 bits (397), Expect = 2e-38
Identities = 72/140 (51%), Positives = 101/140 (72%), Gaps = 2/140 (1%)
Frame = +2
Query: 11 MDVDGDEKIIELKENVKSRKGRGF--DNEQRHDRNTKFEALSDTEDISGPGPQRSVEGWI 184
+D DGD+ I+ LKE K RKGRGF D+ R ++ ++ +D PGPQRSVEGWI
Sbjct: 15 VDEDGDQGIVRLKEKAKHRKGRGFGSDSNTREAIHSYERVRNEDDDELEPGPQRSVEGWI 74
Query: 185 IFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIEYETFAEAQNALKTM 364
+F+ +IHEEA E+++++KF ++GEIKN+H+N DRRTG+ KGY L+EYET +A A + +
Sbjct: 75 LFVTSIHEEAQEDEIQEKFCDYGEIKNIHLNLDRRTGFSKGYALVEYETHKQALAAKEAL 134
Query: 365 SGEEMYGEKINVDWAFVKGP 424
+G E+ G+ I VDW FVKGP
Sbjct: 135 NGAEIMGQTIQVDWCFVKGP 154
>sp|Q8C7E9|CSTFT_MOUSE Cleavage stimulation factor, 64 kDa subunit, tau variant (CSTF 64
kDa subunit, tau variant) (CF-1 64 kDa subunit, tau
variant) (TauCstF-64)
Length = 632
Score = 63.2 bits (152), Expect = 4e-10
Identities = 32/87 (36%), Positives = 49/87 (56%)
Frame = +2
Query: 143 ISGPGPQRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIE 322
+ P RS+ +F+ NI EATEE ++D FSE G + + + +DR TG KGY E
Sbjct: 6 VRDPAMDRSLRS--VFVGNIPYEATEEQLKDIFSEVGSVVSFRLVYDRETGKPKGYGFCE 63
Query: 323 YETFAEAQNALKTMSGEEMYGEKINVD 403
Y+ A +A++ ++G E G + VD
Sbjct: 64 YQDQETALSAMRNLNGREFSGRALRVD 90
>sp|Q9H0L4|CSTFT_HUMAN Cleavage stimulation factor, 64 kDa subunit, tau variant (CSTF 64
kDa subunit, tau variant) (CF-1 64 kDa subunit, tau
variant) (TauCstF-64)
Length = 616
Score = 63.2 bits (152), Expect = 4e-10
Identities = 32/87 (36%), Positives = 49/87 (56%)
Frame = +2
Query: 143 ISGPGPQRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIE 322
+ P RS+ +F+ NI EATEE ++D FSE G + + + +DR TG KGY E
Sbjct: 6 VRDPAMDRSLRS--VFVGNIPYEATEEQLKDIFSEVGSVVSFRLVYDRETGKPKGYGFCE 63
Query: 323 YETFAEAQNALKTMSGEEMYGEKINVD 403
Y+ A +A++ ++G E G + VD
Sbjct: 64 YQDQETALSAMRNLNGREFSGRALRVD 90
>sp|Q8BIQ5|CSTF2_MOUSE Cleavage stimulation factor, 64 kDa subunit (CSTF 64 kDa subunit)
(CF-1 64 kDa subunit) (CstF-64)
Length = 580
Score = 62.8 bits (151), Expect = 5e-10
Identities = 32/87 (36%), Positives = 49/87 (56%)
Frame = +2
Query: 143 ISGPGPQRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIE 322
+ P RS+ +F+ NI EATEE ++D FSE G + + + +DR TG KGY E
Sbjct: 6 VRDPAVDRSLRS--VFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCE 63
Query: 323 YETFAEAQNALKTMSGEEMYGEKINVD 403
Y+ A +A++ ++G E G + VD
Sbjct: 64 YQDQETALSAMRNLNGREFSGRALRVD 90
>sp|P33240|CSTF2_HUMAN Cleavage stimulation factor, 64 kDa subunit (CSTF 64 kDa subunit)
(CF-1 64 kDa subunit) (CstF-64)
Length = 577
Score = 62.8 bits (151), Expect = 5e-10
Identities = 32/87 (36%), Positives = 49/87 (56%)
Frame = +2
Query: 143 ISGPGPQRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIE 322
+ P RS+ +F+ NI EATEE ++D FSE G + + + +DR TG KGY E
Sbjct: 6 VRDPAVDRSLRS--VFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCE 63
Query: 323 YETFAEAQNALKTMSGEEMYGEKINVD 403
Y+ A +A++ ++G E G + VD
Sbjct: 64 YQDQETALSAMRNLNGREFSGRALRVD 90
>sp|Q5RDA3|CSTF2_PONPY Cleavage stimulation factor, 64 kDa subunit (CSTF 64 kDa subunit)
(CF-1 64 kDa subunit) (CstF-64)
Length = 577
Score = 62.8 bits (151), Expect = 5e-10
Identities = 32/87 (36%), Positives = 49/87 (56%)
Frame = +2
Query: 143 ISGPGPQRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIE 322
+ P RS+ +F+ NI EATEE ++D FSE G + + + +DR TG KGY E
Sbjct: 6 VRDPAVDRSLRS--VFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCE 63
Query: 323 YETFAEAQNALKTMSGEEMYGEKINVD 403
Y+ A +A++ ++G E G + VD
Sbjct: 64 YQDQETALSAMRNLNGREFSGRALRVD 90
>sp|Q8HXM1|CSTF2_BOVIN Cleavage stimulation factor, 64 kDa subunit (CSTF 64 kDa subunit)
(CF-1 64 kDa subunit) (CstF-64)
Length = 572
Score = 62.8 bits (151), Expect = 5e-10
Identities = 32/87 (36%), Positives = 49/87 (56%)
Frame = +2
Query: 143 ISGPGPQRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIE 322
+ P RS+ +F+ NI EATEE ++D FSE G + + + +DR TG KGY E
Sbjct: 6 VRDPAVDRSLRS--VFVGNIPYEATEEQLKDIFSEVGPVVSFRLVYDRETGKPKGYGFCE 63
Query: 323 YETFAEAQNALKTMSGEEMYGEKINVD 403
Y+ A +A++ ++G E G + VD
Sbjct: 64 YQDQETALSAMRNLNGREFSGRALRVD 90
>sp|P52912|TIA1_MOUSE Nucleolysin TIA-1 (RNA-binding protein TIA-1)
Length = 386
Score = 61.2 bits (147), Expect = 1e-09
Identities = 31/98 (31%), Positives = 53/98 (54%)
Frame = +2
Query: 131 DTEDISGPGPQRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGY 310
DT + QRS + + +F+ ++ E T ED++ F+ FG I + + D TG KGY
Sbjct: 90 DTSSSTVVSTQRSQDHFHVFVGDLSPEITTEDIKAAFAPFGRISDARVVKDMATGKSKGY 149
Query: 311 VLIEYETFAEAQNALKTMSGEEMYGEKINVDWAFVKGP 424
+ + +A+NA++ M G+ + G +I +WA K P
Sbjct: 150 GFVSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPP 187
Score = 43.5 bits (101), Expect = 3e-04
Identities = 22/75 (29%), Positives = 38/75 (50%)
Frame = +2
Query: 185 IFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIEYETFAEAQNALKTM 364
+++ N+ + TE + FS+ G KN M D T Y +E+ A AL M
Sbjct: 9 LYVGNLSRDVTEALILQLFSQIGPCKNCKMIMD--TAGNDPYCFVEFHEHRHAAAALAAM 66
Query: 365 SGEEMYGEKINVDWA 409
+G ++ G+++ V+WA
Sbjct: 67 NGRKIMGKEVKVNWA 81
Score = 35.4 bits (80), Expect = 0.088
Identities = 25/103 (24%), Positives = 46/103 (44%)
Frame = +2
Query: 98 HDRNTKFEALSDTEDISGPGPQRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMN 277
++ NTK LS E +S P ++ + TE+ +R FS FG+I + +
Sbjct: 193 YESNTK--QLSYDEVVSQSSPNNCT----VYCGGVTSGLTEQLMRQTFSPFGQIMEIRVF 246
Query: 278 FDRRTGYIKGYVLIEYETFAEAQNALKTMSGEEMYGEKINVDW 406
D KGY + + + A +A+ +++G + G + W
Sbjct: 247 PD------KGYSFVRFSSHESAAHAIVSVNGTTIEGHVVKCYW 283
>sp|P31483|TIA1_HUMAN Nucleolysin TIA-1 isoform p40 (RNA-binding protein TIA-1)
(p40-TIA-1) [Contains: Nucleolysin TIA-1 isoform p15
(p15-TIA-1)]
Length = 386
Score = 59.3 bits (142), Expect = 6e-09
Identities = 30/98 (30%), Positives = 52/98 (53%)
Frame = +2
Query: 131 DTEDISGPGPQRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGY 310
DT + QRS + +F+ ++ + T ED++ F+ FG I + + D TG KGY
Sbjct: 90 DTSSSTVVSTQRSQNHFHVFVGDLSPQITTEDIKAAFAPFGRISDARVVKDMATGKSKGY 149
Query: 311 VLIEYETFAEAQNALKTMSGEEMYGEKINVDWAFVKGP 424
+ + +A+NA++ M G+ + G +I +WA K P
Sbjct: 150 GFVSFFNKWDAENAIQQMGGQWLGGRQIRTNWATRKPP 187
Score = 43.5 bits (101), Expect = 3e-04
Identities = 22/75 (29%), Positives = 38/75 (50%)
Frame = +2
Query: 185 IFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIEYETFAEAQNALKTM 364
+++ N+ + TE + FS+ G KN M D T Y +E+ A AL M
Sbjct: 9 LYVGNLSRDVTEALILQLFSQIGPCKNCKMIMD--TAGNDPYCFVEFHEHRHAAAALAAM 66
Query: 365 SGEEMYGEKINVDWA 409
+G ++ G+++ V+WA
Sbjct: 67 NGRKIMGKEVKVNWA 81
Score = 34.3 bits (77), Expect = 0.20
Identities = 19/82 (23%), Positives = 37/82 (45%)
Frame = +2
Query: 161 QRSVEGWIIFIRNIHEEATEEDVRDKFSEFGEIKNLHMNFDRRTGYIKGYVLIEYETFAE 340
Q S ++ + TE+ +R FS FG+I + + D KGY + + +
Sbjct: 208 QSSPSNCTVYCGGVTSGLTEQLMRQTFSPFGQIMEIRVFPD------KGYSFVRFNSHES 261
Query: 341 AQNALKTMSGEEMYGEKINVDW 406
A +A+ +++G + G + W
Sbjct: 262 AAHAIVSVNGTTIEGHVVKCYW 283
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 49,616,516
Number of Sequences: 369166
Number of extensions: 860126
Number of successful extensions: 2560
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2372
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2513
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3650218350
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)