Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_02374
(487 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinas... 102 6e-22
sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinas... 99 4e-21
sp|Q12701|KSG1_SCHPO Serine/threonine-protein kinase ksg1 97 3e-20
sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinas... 97 3e-20
sp|P28829|BYR2_SCHPO Protein kinase byr2 (Protein kinase st... 97 3e-20
sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinas... 97 3e-20
sp|Q10056|SHK2_SCHPO Serine/threonine-protein kinase shk2 96 6e-20
sp|Q9Y6R4|M3K4_HUMAN Mitogen-activated protein kinase kinas... 96 6e-20
sp|O08648|M3K4_MOUSE Mitogen-activated protein kinase kinas... 95 1e-19
sp|Q13188|STK3_HUMAN Serine/threonine-protein kinase 3 (STE... 94 1e-19
>sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 619
Score = 102 bits (253), Expect = 6e-22
Identities = 56/143 (39%), Positives = 84/143 (58%)
Frame = +3
Query: 6 IIHRDLKGPKFLLDDNFNLKLCGFVSGKILKGVSVEDFRNAATQSVVGTANWMAPEVING 185
I+HRD+KG L D N+KL F + K L+ + + +SV GT WM+PEVI+G
Sbjct: 479 IVHRDIKGANILRDSTGNIKLGDFGASKRLQTICLS---GTGMKSVTGTPYWMSPEVISG 535
Query: 186 EKYGSSADIWSFGAIMVEMMTGKPPYSCFASFQVIFKIGMSKKLEFAYPPYCSDKLISFV 365
E YG ADIWS +VEM+T KPP++ F + IFKI ++ PP+ SD F+
Sbjct: 536 EGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIA-TQPTNPKLPPHVSDYTRDFL 594
Query: 366 EPCLDIDPMKRPTVKELMKHEFL 434
+ + ++ RP+ +EL++H F+
Sbjct: 595 KR-IFVEAKLRPSAEELLRHMFV 616
>sp|Q9Y2U5|M3K2_HUMAN Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 618
Score = 99.4 bits (246), Expect = 4e-21
Identities = 55/143 (38%), Positives = 83/143 (58%)
Frame = +3
Query: 6 IIHRDLKGPKFLLDDNFNLKLCGFVSGKILKGVSVEDFRNAATQSVVGTANWMAPEVING 185
I+HRD+KG L D N+KL F + K L+ + + +SV GT WM+PEVI+G
Sbjct: 478 ILHRDIKGANILRDSTGNVKLGDFGASKRLQTICLS---GTGMKSVTGTPYWMSPEVISG 534
Query: 186 EKYGSSADIWSFGAIMVEMMTGKPPYSCFASFQVIFKIGMSKKLEFAYPPYCSDKLISFV 365
+ YG ADIWS +VEM+T KPP++ F + IFKI ++ PP+ SD F+
Sbjct: 535 QGYGRKADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIA-TQPTNPKLPPHVSDYTRDFL 593
Query: 366 EPCLDIDPMKRPTVKELMKHEFL 434
+ + ++ RP+ EL++H F+
Sbjct: 594 KR-IFVEAKLRPSADELLRHMFV 615
>sp|Q12701|KSG1_SCHPO Serine/threonine-protein kinase ksg1
Length = 592
Score = 96.7 bits (239), Expect = 3e-20
Identities = 58/153 (37%), Positives = 85/153 (55%), Gaps = 11/153 (7%)
Frame = +3
Query: 6 IIHRDLKGPKFLLDDNFNLKLCGFVSGKILK---GVSVEDFRNAA-----TQSVVGTANW 161
+IHRDLK LLDDN K+ F S KIL G ED +A ++S VGTA +
Sbjct: 219 VIHRDLKPENILLDDNMRTKITDFGSAKILNSSHGSHEEDTHHADKPQAHSRSFVGTARY 278
Query: 162 MAPEVINGEKYGSSADIWSFGAIMVEMMTGKPPYSC---FASFQVIFKIGMSKKLEFAYP 332
++PEV++ + G+++DIW+FG I+ +M+ GKPP+ + +FQ I L + P
Sbjct: 279 VSPEVLSDKIAGTASDIWAFGCILFQMLAGKPPFVAGNEYLTFQSIL------HLSYEIP 332
Query: 333 PYCSDKLISFVEPCLDIDPMKRPTVKELMKHEF 431
P SD ++ L +DP R TV E+ +H F
Sbjct: 333 PDISDVASDLIKKLLVLDPKDRLTVDEIHQHPF 365
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 96.7 bits (239), Expect = 3e-20
Identities = 53/142 (37%), Positives = 82/142 (57%)
Frame = +3
Query: 6 IIHRDLKGPKFLLDDNFNLKLCGFVSGKILKGVSVEDFRNAATQSVVGTANWMAPEVING 185
I+HRD+KG L D N+KL F + K L+ + + +SV GT WM+PEVI+G
Sbjct: 485 IVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMS---GTGIRSVTGTPYWMSPEVISG 541
Query: 186 EKYGSSADIWSFGAIMVEMMTGKPPYSCFASFQVIFKIGMSKKLEFAYPPYCSDKLISFV 365
E YG AD+WS G +VEM+T KPP++ + + IFKI ++ P + S+ F+
Sbjct: 542 EGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIA-TQPTNPQLPSHISEHGRDFL 600
Query: 366 EPCLDIDPMKRPTVKELMKHEF 431
+ ++ +RP+ +EL+ H F
Sbjct: 601 RR-IFVEARQRPSAEELLTHHF 621
>sp|P28829|BYR2_SCHPO Protein kinase byr2 (Protein kinase ste8) (MAPK kinase kinase)
(MAPKKK)
Length = 659
Score = 96.7 bits (239), Expect = 3e-20
Identities = 54/143 (37%), Positives = 74/143 (51%)
Frame = +3
Query: 6 IIHRDLKGPKFLLDDNFNLKLCGFVSGKILKGVSVEDFRNAATQSVVGTANWMAPEVING 185
I+HRD+KG L+D+ +K+ F K L+ S A S G++ WMAPEV+
Sbjct: 518 IVHRDIKGANILVDNKGKIKISDFGISKKLELNSTSTKTGGARPSFQGSSFWMAPEVVKQ 577
Query: 186 EKYGSSADIWSFGAIMVEMMTGKPPYSCFASFQVIFKIGMSKKLEFAYPPYCSDKLISFV 365
+ DIWS G +++EM+T K PY Q IF+IG + EF P S I F+
Sbjct: 578 TMHTEKTDIWSLGCLVIEMLTSKHPYPNCDQMQAIFRIGENILPEF--PSNISSSAIDFL 635
Query: 366 EPCLDIDPMKRPTVKELMKHEFL 434
E ID RPT EL+ H F+
Sbjct: 636 EKTFAIDCNLRPTASELLSHPFV 658
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 96.7 bits (239), Expect = 3e-20
Identities = 53/142 (37%), Positives = 82/142 (57%)
Frame = +3
Query: 6 IIHRDLKGPKFLLDDNFNLKLCGFVSGKILKGVSVEDFRNAATQSVVGTANWMAPEVING 185
I+HRD+KG L D N+KL F + K L+ + + +SV GT WM+PEVI+G
Sbjct: 485 IVHRDIKGANILRDSAGNVKLGDFGASKRLQTICMS---GTGMRSVTGTPYWMSPEVISG 541
Query: 186 EKYGSSADIWSFGAIMVEMMTGKPPYSCFASFQVIFKIGMSKKLEFAYPPYCSDKLISFV 365
E YG AD+WS G +VEM+T KPP++ + + IFKI ++ P + S+ F+
Sbjct: 542 EGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIA-TQPTNPQLPSHISEHGRDFL 600
Query: 366 EPCLDIDPMKRPTVKELMKHEF 431
+ ++ +RP+ +EL+ H F
Sbjct: 601 RR-IFVEARQRPSAEELLTHHF 621
>sp|Q10056|SHK2_SCHPO Serine/threonine-protein kinase shk2
Length = 589
Score = 95.5 bits (236), Expect = 6e-20
Identities = 53/144 (36%), Positives = 75/144 (52%)
Frame = +3
Query: 3 NIIHRDLKGPKFLLDDNFNLKLCGFVSGKILKGVSVEDFRNAATQSVVGTANWMAPEVIN 182
NIIHRD+K LLD++ N+K+ F L R ++VGT WMAPEV+
Sbjct: 429 NIIHRDIKSDNVLLDNSGNIKITDFGFCARLSN------RTNKRVTMVGTPYWMAPEVVK 482
Query: 183 GEKYGSSADIWSFGAIMVEMMTGKPPYSCFASFQVIFKIGMSKKLEFAYPPYCSDKLISF 362
+YG+ DIWS G +++EM+ +PPY + ++ I + P S L SF
Sbjct: 483 QNEYGTKVDIWSLGIMIIEMIENEPPYLREDPIRALYLIAKNGTPTLKKPNLVSKNLKSF 542
Query: 363 VEPCLDIDPMKRPTVKELMKHEFL 434
+ CL ID + R T EL+ H FL
Sbjct: 543 LNSCLTIDTIFRATAAELLTHSFL 566
>sp|Q9Y6R4|M3K4_HUMAN Mitogen-activated protein kinase kinase kinase 4 (MAPK/ERK kinase
kinase 4) (MEK kinase 4) (MEKK 4) (MAP three kinase 1)
Length = 1607
Score = 95.5 bits (236), Expect = 6e-20
Identities = 60/153 (39%), Positives = 86/153 (56%), Gaps = 9/153 (5%)
Frame = +3
Query: 6 IIHRDLKGPKFLLDDNFNLKLCGFVSGKILKGVSVEDFRNAATQ-----SVVGTANWMAP 170
I+HRD+KG L + +KL F G SV+ NA T S +GTA +MAP
Sbjct: 1458 IVHRDIKGANIFLTSSGLIKLGDF-------GCSVKLKNNAQTMPGEVNSTLGTAAYMAP 1510
Query: 171 EVIN---GEKYGSSADIWSFGAIMVEMMTGKPPYSCFA-SFQVIFKIGMSKKLEFAYPPY 338
EVI GE +G +ADIWS G +++EM+TGK P+ + +FQ+++K+GM K P
Sbjct: 1511 EVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPI--PER 1568
Query: 339 CSDKLISFVEPCLDIDPMKRPTVKELMKHEFLK 437
S + F+ CL+ DP R T +L+ H F+K
Sbjct: 1569 LSPEGKDFLSHCLESDPKMRWTASQLLDHSFVK 1601
>sp|O08648|M3K4_MOUSE Mitogen-activated protein kinase kinase kinase 4 (MAPK/ERK kinase
kinase 4) (MEK kinase 4) (MEKK 4)
Length = 1597
Score = 94.7 bits (234), Expect = 1e-19
Identities = 60/153 (39%), Positives = 87/153 (56%), Gaps = 9/153 (5%)
Frame = +3
Query: 6 IIHRDLKGPKFLLDDNFNLKLCGFVSGKILKGVSVEDFRNAATQ-----SVVGTANWMAP 170
I+HRD+KG L + +KL F G SV+ NA T S +GTA +MAP
Sbjct: 1448 IVHRDIKGANIFLTSSGLIKLGDF-------GCSVKLKNNAQTMPGEVNSTLGTAAYMAP 1500
Query: 171 EVIN---GEKYGSSADIWSFGAIMVEMMTGKPPYSCFA-SFQVIFKIGMSKKLEFAYPPY 338
EVI GE +G +ADIWS G +++EM+TGK P+ + +FQ+++K+GM K P
Sbjct: 1501 EVITRAKGEGHGRAADIWSLGCVVIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPI--PER 1558
Query: 339 CSDKLISFVEPCLDIDPMKRPTVKELMKHEFLK 437
S + +F+ CL+ DP R T +L+ H F+K
Sbjct: 1559 LSPEGKAFLSHCLESDPKIRWTASQLLDHAFVK 1591
>sp|Q13188|STK3_HUMAN Serine/threonine-protein kinase 3 (STE20-like kinase MST2) (MST-2)
(Mammalian STE20-like protein kinase 2)
(Serine/threonine-protein kinase Krs-1)
Length = 491
Score = 94.4 bits (233), Expect = 1e-19
Identities = 55/144 (38%), Positives = 75/144 (52%), Gaps = 1/144 (0%)
Frame = +3
Query: 9 IHRDLKGPKFLLDDNFNLKLCGF-VSGKILKGVSVEDFRNAATQSVVGTANWMAPEVING 185
IHRD+K LL+ + KL F V+G++ + A +V+GT WMAPEVI
Sbjct: 143 IHRDIKAGNILLNTEGHAKLADFGVAGQLTDTM-------AKRNTVIGTPFWMAPEVIQE 195
Query: 186 EKYGSSADIWSFGAIMVEMMTGKPPYSCFASFQVIFKIGMSKKLEFAYPPYCSDKLISFV 365
Y ADIWS G +EM GKPPY+ + IF I + F P SD FV
Sbjct: 196 IGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFV 255
Query: 366 EPCLDIDPMKRPTVKELMKHEFLK 437
+ CL +P +R T +L++H F+K
Sbjct: 256 KKCLVKNPEQRATATQLLQHPFIK 279
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,281,529
Number of Sequences: 369166
Number of extensions: 1180497
Number of successful extensions: 6470
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4726
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4990
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 2921208590
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)