Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_02279
(799 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q64191|ASPG_MOUSE N(4)-(beta-N-acetylglucosaminyl)-L-asp... 207 3e-53
sp|P20933|ASPG_HUMAN N(4)-(beta-N-acetylglucosaminyl)-L-asp... 205 1e-52
sp|O02467|ASPG_SPOFR N(4)-(Beta-N-acetylglucosaminyl)-L-asp... 204 2e-52
sp|Q21697|ASPG_CAEEL Putative N(4)-(beta-N-acetylglucosamin... 179 6e-45
sp|Q47898|ASPG_FLAME N(4)-(Beta-N-acetylglucosaminyl)-L-asp... 111 2e-24
sp|Q9V262|ASGX_PYRAB Putative L-asparaginase (L-asparagine ... 73 1e-12
sp|Q5JHT1|ASGX_PYRKO Putative L-asparaginase (L-asparagine ... 70 7e-12
sp|Q8U4E6|ASGX_PYRFU Putative L-asparaginase (L-asparagine ... 69 1e-11
sp|O57971|ASGX_PYRHO Putative L-asparaginase (L-asparagine ... 68 3e-11
sp|P37595|ASGX_ECOLI Putative L-asparaginase precursor (L-a... 58 3e-08
>sp|Q64191|ASPG_MOUSE N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase precursor
(Glycosylasparaginase) (Aspartylglucosaminidase)
(N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase)
(AGA) [Contains: Glycosylasparaginase alpha chain;
Glycosylasparaginase beta chain]
Length = 346
Score = 207 bits (527), Expect = 3e-53
Identities = 115/248 (46%), Positives = 147/248 (59%), Gaps = 4/248 (1%)
Frame = +1
Query: 7 VKNAIKVAHYVLKYTRHTLLVGDSAAEFAKEMGFTWEDLSTNNSSKLWKEWKEKNCQPNF 186
+KNAI VA VL++T HTLLVGDSA +FA+ MGFT EDLST S L +W +NCQPN+
Sbjct: 108 IKNAIGVARRVLEHTTHTLLVGDSATKFAESMGFTNEDLSTKTSRDLHSDWLSRNCQPNY 167
Query: 187 RRNVSPDPTKHCGPYKPLSRNHKSIERKDATNDNNRVAGTVDEHHHDTIGMIALNRNGSM 366
RNV PDP+K+CGPYKP +SI VD H HDTIGM+ +++ G
Sbjct: 168 WRNVIPDPSKYCGPYKPSGFLKQSISPHKE---------EVDIHSHDTIGMVVIHKTGHT 218
Query: 367 AVGTSTNGAIFKVPGRVGDTAIPGSGGYXXXXXXXXXXXXXXXXLMRFLLSYQAVEFMRQ 546
A GTSTNG FK+PGRVGD+ IPG+G Y L+RFL SYQAVE+MR
Sbjct: 219 AAGTSTNGIKFKIPGRVGDSPIPGAGAYADDTAGAAAATGDGDTLLRFLPSYQAVEYMRG 278
Query: 547 GMHPNKACVESLRRV-KQPGVWYGALLAMNNAGEYGAAC---VGFTNYYISIRNAKTRNT 714
G P AC + + R+ K ++GA++ + G YGAAC FT + + N+ N
Sbjct: 279 GDDPAIACQKVILRIQKYYPNFFGAVICASVNGSYGAACNKLPTFTQFSFMVSNS-LHNE 337
Query: 715 TVTTKIDC 738
K+DC
Sbjct: 338 PTEKKVDC 345
>sp|P20933|ASPG_HUMAN N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase precursor
(Glycosylasparaginase) (Aspartylglucosaminidase)
(N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase)
(AGA) [Contains: Glycosylasparaginase alpha chain;
Glycosylasparaginase beta chain]
Length = 346
Score = 205 bits (521), Expect = 1e-52
Identities = 113/248 (45%), Positives = 150/248 (60%), Gaps = 4/248 (1%)
Frame = +1
Query: 7 VKNAIKVAHYVLKYTRHTLLVGDSAAEFAKEMGFTWEDLSTNNSSKLWKEWKEKNCQPNF 186
+KNAI VA VL++T HTLLVG+SA FA+ MGF EDLST+ S L +W +NCQPN+
Sbjct: 108 IKNAIGVARKVLEHTTHTLLVGESATTFAQSMGFINEDLSTSASQALHSDWLARNCQPNY 167
Query: 187 RRNVSPDPTKHCGPYKPLSRNHKSIERKDATNDNNRVAGTVDEHHHDTIGMIALNRNGSM 366
RNV PDP+K+CGPYKP I ++D T D+ HDTIGM+ +++ G +
Sbjct: 168 WRNVIPDPSKYCGPYKP-----PGILKQDIPIHKE----TEDDRGHDTIGMVVIHKTGHI 218
Query: 367 AVGTSTNGAIFKVPGRVGDTAIPGSGGYXXXXXXXXXXXXXXXXLMRFLLSYQAVEFMRQ 546
A GTSTNG FK+ GRVGD+ IPG+G Y LMRFL SYQAVE+MR+
Sbjct: 219 AAGTSTNGIKFKIHGRVGDSPIPGAGAYADDTAGAAAATGNGDILMRFLPSYQAVEYMRR 278
Query: 547 GMHPNKACVESLRRV-KQPGVWYGALLAMNNAGEYGAAC---VGFTNYYISIRNAKTRNT 714
G P AC + + R+ K ++GA++ N G YGAAC FT + + N++ +N
Sbjct: 279 GEDPTIACQKVISRIQKHFPEFFGAVICANVTGSYGAACNKLSTFTQFSFMVYNSE-KNQ 337
Query: 715 TVTTKIDC 738
K+DC
Sbjct: 338 PTEEKVDC 345
>sp|O02467|ASPG_SPOFR N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase
(Glycosylasparaginase) (Aspartylglucosaminidase)
(N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase)
(AGA) [Contains: Glycosylasparaginase alpha chain;
Glycosylasparaginase beta chain]
Length = 320
Score = 204 bits (520), Expect = 2e-52
Identities = 110/246 (44%), Positives = 151/246 (61%), Gaps = 2/246 (0%)
Frame = +1
Query: 7 VKNAIKVAHYVLKYTRHTLLVGDSAAEFAKEMGFTWEDLSTNNSSKLWKEWK-EKNCQPN 183
+K+AI VA +V++YT H+ L G+ A +FA EMGF E LST+ S +LW +W+ EK CQPN
Sbjct: 85 IKSAISVARHVMEYTTHSFLAGELATKFAVEMGFKEESLSTDESRELWSKWRFEKQCQPN 144
Query: 184 FRRNVSPDPTKHCGPYKPLSRNHKSIERKDATNDNNRVAGTVDEHHHDTIGMIALNRNGS 363
FR+NV PDP KHCGPY HK D + V VD+++HDTIGM+A++ G
Sbjct: 145 FRKNVKPDPRKHCGPY------HKKRNFVDYIHPEVFV---VDQYNHDTIGMVAVDSKGD 195
Query: 364 MAVGTSTNGAIFKVPGRVGDTAIPGSGGYXXXXXXXXXXXXXXXXLMRFLLSYQAVEFMR 543
+A GTSTNGA FK+PGRVGD+ IPG+G Y +MRFL S+ AVE MR
Sbjct: 196 VAAGTSTNGAKFKIPGRVGDSPIPGAGAYADNTVGGAAATGNGDTMMRFLPSFLAVEEMR 255
Query: 544 QGMHPNKACVESLRRVK-QPGVWYGALLAMNNAGEYGAACVGFTNYYISIRNAKTRNTTV 720
+G P A +++R+ + GA++A++ G+YGAAC G + +++ KTR T
Sbjct: 256 RGASPANAAKTAIKRISAHHPDFMGAVIALSKNGQYGAACNGIETFPFVVQD-KTRKTFE 314
Query: 721 TTKIDC 738
I C
Sbjct: 315 VVTIKC 320
>sp|Q21697|ASPG_CAEEL Putative N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase precursor
(Glycosylasparaginase) (Aspartylglucosaminidase)
(N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase)
(AGA) [Contains: Glycosylasparaginase alpha chain;
Glycosylasparaginase beta chain]
Length = 363
Score = 179 bits (455), Expect = 6e-45
Identities = 101/247 (40%), Positives = 144/247 (58%), Gaps = 3/247 (1%)
Frame = +1
Query: 7 VKNAIKVAHYVLKYTRHTLLVGDSAAEFAKEMGFTWEDLSTNNSSKLWKEWKEKNCQPNF 186
+++A +VA V+ +T+HTLLVG+SA +FAK +GF EDLST + +WK + CQPNF
Sbjct: 106 IRDAARVAWGVMNFTKHTLLVGESATQFAKTLGFKEEDLSTEETKSWISKWKTEKCQPNF 165
Query: 187 RRNVSPDPTKHCGPYK--PLSRNHKSIERKDATNDNNRVAGTVDEHHHDTIGMIALNRNG 360
+NVSPDP+ CGPYK PL+ KS+ N ++ V++ +HDTIGM+ +
Sbjct: 166 WKNVSPDPSSSCGPYKTNPLT---KSMRYYSLVNQSDEAGYLVEKTNHDTIGMVVRDTEN 222
Query: 361 SMAVGTSTNGAIFKVPGRVGDTAIPGSGGYXXXXXXXXXXXXXXXXLMRFLLSYQAVEFM 540
+ GTS+NGA FK+PGRVGD+ IPG+G Y +MRFL S+ AV M
Sbjct: 223 IFSAGTSSNGARFKIPGRVGDSPIPGAGAY-ANKFGGAAATGDGDVMMRFLPSFFAVTQM 281
Query: 541 RQGMHPNKACVESLRRV-KQPGVWYGALLAMNNAGEYGAACVGFTNYYISIRNAKTRNTT 717
G P+KA +++ R+ K + GA++AMN G GA+C + ++ A T
Sbjct: 282 ELGTKPSKAAYKAITRILKVFPKFSGAVVAMNVKGRIGASCANINKFGYNV--AFQNGTV 339
Query: 718 VTTKIDC 738
VT I C
Sbjct: 340 VTYSISC 346
>sp|Q47898|ASPG_FLAME N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase precursor
(Glycosylasparaginase) (Aspartylglucosaminidase)
(N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase)
(AGA) [Contains: Glycosylasparaginase alpha chain;
Glycosylasparaginase beta chain]
Length = 340
Score = 111 bits (278), Expect = 2e-24
Identities = 73/228 (32%), Positives = 107/228 (46%), Gaps = 8/228 (3%)
Frame = +1
Query: 4 HVKNAIKVAHYVLKYTRHTLLVGDSAAEFAKEMGFTWEDLSTNNSSKLWKEWKEKNCQPN 183
H+KN I VA V++ T H +LVGD A EFA GF E+L T S K WKEW + +
Sbjct: 129 HIKNPISVARAVMEKTPHVMLVGDGALEFALSQGFKKENLLTAESEKEWKEWLKTS---- 184
Query: 184 FRRNVSPDPTKHCGPYKPLSRNHKSIERKDATNDNNRVAGTVDEHHHDTIGMIALNRNGS 363
YKP+ V+ +HDTIGMIAL+ G+
Sbjct: 185 --------------QYKPI----------------------VNIENHDTIGMIALDAQGN 208
Query: 364 MAVGTSTNGAIFKVPGRVGDTAIPGSGGYXXXXXXXXXXXXXXXXLMRFLLSYQAVEFMR 543
++ +T+G +K+ GRVGD+ I G+G + ++R + ++ VE M
Sbjct: 209 LSGACTTSGMAYKMHGRVGDSPIIGAGLFVDNEIGAATATGHGEEVIRTVGTHLVVELMN 268
Query: 544 QGMHPNKACVESLRRVKQPGVWYG--------ALLAMNNAGEYGAACV 663
QG P +AC E++ R+ + G +A+N GEYGA C+
Sbjct: 269 QGRTPQQACKEAVERIVKIVNRRGKNLKDIQVGFIALNKKGEYGAYCI 316
>sp|Q9V262|ASGX_PYRAB Putative L-asparaginase (L-asparagine amidohydrolase)
Length = 305
Score = 72.8 bits (177), Expect = 1e-12
Identities = 56/187 (29%), Positives = 79/187 (42%)
Frame = +1
Query: 7 VKNAIKVAHYVLKYTRHTLLVGDSAAEFAKEMGFTWEDLSTNNSSKLWKEWKEKNCQPNF 186
VKN I VA V++ T H LLVG+ A +FA+ MGF D +T K W+E KEK
Sbjct: 96 VKNPISVARKVMEKTDHVLLVGEGAVKFARIMGFPEYDPTTEERRKQWQELKEK------ 149
Query: 187 RRNVSPDPTKHCGPYKPLSRNHKSIERKDATNDNNRVAGTVDEHHHDTIGMIALNRNGSM 366
+ +H L + H + R T+G +A + +
Sbjct: 150 ---LMKGEVRHWKKLGELIKEHPEVLR-------------------STVGAVAFD-GEEV 186
Query: 367 AVGTSTNGAIFKVPGRVGDTAIPGSGGYXXXXXXXXXXXXXXXXLMRFLLSYQAVEFMRQ 546
GTST G K+ GRVGDT I G+G Y + + L+ A +F+R
Sbjct: 187 VAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAI-KLALAKTATDFVRL 245
Query: 547 GMHPNKA 567
G+ A
Sbjct: 246 GLDAQAA 252
>sp|Q5JHT1|ASGX_PYRKO Putative L-asparaginase (L-asparagine amidohydrolase)
Length = 306
Score = 70.1 bits (170), Expect = 7e-12
Identities = 54/187 (28%), Positives = 78/187 (41%)
Frame = +1
Query: 7 VKNAIKVAHYVLKYTRHTLLVGDSAAEFAKEMGFTWEDLSTNNSSKLWKEWKEKNCQPNF 186
VKN I VA V++ T H LL+G+ A +FA+ +GF D T K W+E ++K
Sbjct: 96 VKNPISVARKVMEKTDHVLLIGEGAVKFARLLGFEEYDPITEERLKQWEELRKK------ 149
Query: 187 RRNVSPDPTKHCGPYKPLSRNHKSIERKDATNDNNRVAGTVDEHHHDTIGMIALNRNGSM 366
+ TKH L + + + R T+G +A + +
Sbjct: 150 --LIEKGETKHWKKLNELIKEYPEVLR-------------------STVGAVAFD-GEEV 187
Query: 367 AVGTSTNGAIFKVPGRVGDTAIPGSGGYXXXXXXXXXXXXXXXXLMRFLLSYQAVEFMRQ 546
GTST G K+ GRVGDT I G G Y + + L+ A +F+R
Sbjct: 188 VAGTSTGGVFLKMFGRVGDTPIIGGGTYANEVAGASCTGLGEVAI-KLALAKSAADFVRL 246
Query: 547 GMHPNKA 567
GM A
Sbjct: 247 GMDAQTA 253
>sp|Q8U4E6|ASGX_PYRFU Putative L-asparaginase (L-asparagine amidohydrolase)
Length = 306
Score = 68.9 bits (167), Expect = 1e-11
Identities = 54/193 (27%), Positives = 83/193 (43%)
Frame = +1
Query: 7 VKNAIKVAHYVLKYTRHTLLVGDSAAEFAKEMGFTWEDLSTNNSSKLWKEWKEKNCQPNF 186
VKN I VA V++ T H LL+G+ A +FA+ MGF D +T K W+E ++K +
Sbjct: 96 VKNPISVARKVMEKTDHVLLIGEGAVKFARLMGFPEYDPTTEERRKQWEELRKKLLETG- 154
Query: 187 RRNVSPDPTKHCGPYKPLSRNHKSIERKDATNDNNRVAGTVDEHHHDTIGMIALNRNGSM 366
+H L + + + R T+G +A + +
Sbjct: 155 -------EIRHWKKLSELIKEYPEVLRS-------------------TVGAVAFDGEEIV 188
Query: 367 AVGTSTNGAIFKVPGRVGDTAIPGSGGYXXXXXXXXXXXXXXXXLMRFLLSYQAVEFMRQ 546
A GTST G K+ GRVGDT I G+G Y + + L+ A +F+R
Sbjct: 189 A-GTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAI-KLSLAKTATDFVRL 246
Query: 547 GMHPNKACVESLR 585
G+ A ++R
Sbjct: 247 GLDAQAASEAAIR 259
>sp|O57971|ASGX_PYRHO Putative L-asparaginase (L-asparagine amidohydrolase)
Length = 305
Score = 67.8 bits (164), Expect = 3e-11
Identities = 56/187 (29%), Positives = 78/187 (41%)
Frame = +1
Query: 7 VKNAIKVAHYVLKYTRHTLLVGDSAAEFAKEMGFTWEDLSTNNSSKLWKEWKEKNCQPNF 186
VKN I VA V++ T H LLVG+ A +FA+ MGF + T + WKE KEK
Sbjct: 96 VKNPISVARKVMEKTDHVLLVGEGAVKFARLMGFPEYNPITEERIEQWKELKEK------ 149
Query: 187 RRNVSPDPTKHCGPYKPLSRNHKSIERKDATNDNNRVAGTVDEHHHDTIGMIALNRNGSM 366
+ K+ L + + + R T+G +A + +
Sbjct: 150 ---LMKGEIKYWKKLGELIKEYPEVLR-------------------STVGAVAFD-GEEI 186
Query: 367 AVGTSTNGAIFKVPGRVGDTAIPGSGGYXXXXXXXXXXXXXXXXLMRFLLSYQAVEFMRQ 546
GTST G K+ GRVGDT I G+G Y + R L+ A +F+R
Sbjct: 187 VAGTSTGGVFLKMFGRVGDTPIIGAGTYANEVAGASCTGLGEVAI-RLALAKTATDFVRL 245
Query: 547 GMHPNKA 567
GM A
Sbjct: 246 GMDAQAA 252
>sp|P37595|ASGX_ECOLI Putative L-asparaginase precursor (L-asparagine amidohydrolase)
Length = 321
Score = 58.2 bits (139), Expect = 3e-08
Identities = 51/214 (23%), Positives = 86/214 (40%), Gaps = 1/214 (0%)
Frame = +1
Query: 4 HVKNAIKVAHYVLKYTRHTLLVGDSAAEFAKEMGFTWEDLSTNNSSKLWKEWKEKNCQPN 183
H++N + A V++ + H +++G+ A FA G
Sbjct: 102 HLRNPVLAARLVMEQSPHVMMIGEGAENFAFARGM------------------------- 136
Query: 184 FRRNVSPDPTKHCGPYKPLSRNHKSIERKDATNDNNRVAGTVDEHHHDTIGMIALNRNGS 363
VSP+ Y+ L K + AT ++ A ++ T+G +AL+ +G+
Sbjct: 137 --ERVSPEIFSTSLRYEQLLAARK----EGATVLDHSGAPLDEKQKMGTVGAVALDLDGN 190
Query: 364 MAVGTSTNGAIFKVPGRVGDTAIPGSGGYXXXXXXXXXXXXXXXXLMRFLLSYQAVEFMR 543
+A TST G K+PGRVGD+ + G+G Y +R L +Y M
Sbjct: 191 LAAATSTGGMTNKLPGRVGDSPLVGAGCYANNASVAVSCTGTGEVFIRALAAYDIAALMD 250
Query: 544 QGMHPNKACVESLRRVKQPGV-WYGALLAMNNAG 642
G E + K P + G L+A+++ G
Sbjct: 251 YGGLSLAEACERVVMEKLPALGGSGGLIAIDHEG 284
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 97,518,152
Number of Sequences: 369166
Number of extensions: 2040019
Number of successful extensions: 6439
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6037
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 6419
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7522142940
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)