Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_02245
(889 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O02467|ASPG_SPOFR N(4)-(Beta-N-acetylglucosaminyl)-L-asp... 143 7e-34
sp|P20933|ASPG_HUMAN N(4)-(beta-N-acetylglucosaminyl)-L-asp... 140 5e-33
sp|Q64191|ASPG_MOUSE N(4)-(beta-N-acetylglucosaminyl)-L-asp... 137 3e-32
sp|Q21697|ASPG_CAEEL Putative N(4)-(beta-N-acetylglucosamin... 118 2e-26
sp|Q47898|ASPG_FLAME N(4)-(Beta-N-acetylglucosaminyl)-L-asp... 95 2e-19
sp|P37595|ASGX_ECOLI Putative L-asparaginase precursor (L-a... 63 1e-09
sp|O57971|ASGX_PYRHO Putative L-asparaginase (L-asparagine ... 57 7e-08
sp|Q5JHT1|ASGX_PYRKO Putative L-asparaginase (L-asparagine ... 56 2e-07
sp|Q8U4E6|ASGX_PYRFU Putative L-asparaginase (L-asparagine ... 56 2e-07
sp|Q9V262|ASGX_PYRAB Putative L-asparaginase (L-asparagine ... 55 3e-07
>sp|O02467|ASPG_SPOFR N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase
(Glycosylasparaginase) (Aspartylglucosaminidase)
(N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase)
(AGA) [Contains: Glycosylasparaginase alpha chain;
Glycosylasparaginase beta chain]
Length = 320
Score = 143 bits (360), Expect = 7e-34
Identities = 70/145 (48%), Positives = 100/145 (68%), Gaps = 1/145 (0%)
Frame = +3
Query: 363 VDEHHHDTIGMIALNRNGSMAVGTSTNGAIFKVPGRVGDTAIPGSGGYVDSTIGGAVGTG 542
VD+++HDTIGM+A++ G +A GTSTNGA FK+PGRVGD+ IPG+G Y D+T+GGA TG
Sbjct: 177 VDQYNHDTIGMVAVDSKGDVAAGTSTNGAKFKIPGRVGDSPIPGAGAYADNTVGGAAATG 236
Query: 543 DGDVLMRFLLSYQAVEFMRQGMHPNKACVESLRRVK-QPGVWYGALLAMNNAGEYGAACV 719
+GD +MRFL S+ AVE MR+G P A +++R+ + GA++A++ G+YGAAC
Sbjct: 237 NGDTMMRFLPSFLAVEEMRRGASPANAAKTAIKRISAHHPDFMGAVIALSKNGQYGAACN 296
Query: 720 GFTNYYISIRNAKTRNTTVTTKIDC 794
G + +++ KTR T I C
Sbjct: 297 GIETFPFVVQD-KTRKTFEVVTIKC 320
Score = 32.0 bits (71), Expect = 2.4
Identities = 11/16 (68%), Positives = 13/16 (81%)
Frame = +3
Query: 3 FPEDVSPDPTKHCGPY 50
F ++V PDP KHCGPY
Sbjct: 145 FRKNVKPDPRKHCGPY 160
>sp|P20933|ASPG_HUMAN N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase precursor
(Glycosylasparaginase) (Aspartylglucosaminidase)
(N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase)
(AGA) [Contains: Glycosylasparaginase alpha chain;
Glycosylasparaginase beta chain]
Length = 346
Score = 140 bits (353), Expect = 5e-33
Identities = 71/149 (47%), Positives = 97/149 (65%), Gaps = 4/149 (2%)
Frame = +3
Query: 360 TVDEHHHDTIGMIALNRNGSMAVGTSTNGAIFKVPGRVGDTAIPGSGGYVDSTIGGAVGT 539
T D+ HDTIGM+ +++ G +A GTSTNG FK+ GRVGD+ IPG+G Y D T G A T
Sbjct: 198 TEDDRGHDTIGMVVIHKTGHIAAGTSTNGIKFKIHGRVGDSPIPGAGAYADDTAGAAAAT 257
Query: 540 GDGDVLMRFLLSYQAVEFMRQGMHPNKACVESLRRV-KQPGVWYGALLAMNNAGEYGAAC 716
G+GD+LMRFL SYQAVE+MR+G P AC + + R+ K ++GA++ N G YGAAC
Sbjct: 258 GNGDILMRFLPSYQAVEYMRRGEDPTIACQKVISRIQKHFPEFFGAVICANVTGSYGAAC 317
Query: 717 ---VGFTNYYISIRNAKTRNTTVTTKIDC 794
FT + + N++ +N K+DC
Sbjct: 318 NKLSTFTQFSFMVYNSE-KNQPTEEKVDC 345
Score = 33.1 bits (74), Expect = 1.1
Identities = 16/34 (47%), Positives = 22/34 (64%), Gaps = 6/34 (17%)
Frame = +3
Query: 12 DVSPDPTKHCGPYKP---LSRN---HKSIESETG 95
+V PDP+K+CGPYKP L ++ HK E + G
Sbjct: 170 NVIPDPSKYCGPYKPPGILKQDIPIHKETEDDRG 203
>sp|Q64191|ASPG_MOUSE N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase precursor
(Glycosylasparaginase) (Aspartylglucosaminidase)
(N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase)
(AGA) [Contains: Glycosylasparaginase alpha chain;
Glycosylasparaginase beta chain]
Length = 346
Score = 137 bits (346), Expect = 3e-32
Identities = 72/148 (48%), Positives = 93/148 (62%), Gaps = 4/148 (2%)
Frame = +3
Query: 363 VDEHHHDTIGMIALNRNGSMAVGTSTNGAIFKVPGRVGDTAIPGSGGYVDSTIGGAVGTG 542
VD H HDTIGM+ +++ G A GTSTNG FK+PGRVGD+ IPG+G Y D T G A TG
Sbjct: 199 VDIHSHDTIGMVVIHKTGHTAAGTSTNGIKFKIPGRVGDSPIPGAGAYADDTAGAAAATG 258
Query: 543 DGDVLMRFLLSYQAVEFMRQGMHPNKACVESLRRV-KQPGVWYGALLAMNNAGEYGAAC- 716
DGD L+RFL SYQAVE+MR G P AC + + R+ K ++GA++ + G YGAAC
Sbjct: 259 DGDTLLRFLPSYQAVEYMRGGDDPAIACQKVILRIQKYYPNFFGAVICASVNGSYGAACN 318
Query: 717 --VGFTNYYISIRNAKTRNTTVTTKIDC 794
FT + + N+ N K+DC
Sbjct: 319 KLPTFTQFSFMVSNS-LHNEPTEKKVDC 345
Score = 32.3 bits (72), Expect = 1.8
Identities = 11/15 (73%), Positives = 14/15 (93%)
Frame = +3
Query: 12 DVSPDPTKHCGPYKP 56
+V PDP+K+CGPYKP
Sbjct: 170 NVIPDPSKYCGPYKP 184
>sp|Q21697|ASPG_CAEEL Putative N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase precursor
(Glycosylasparaginase) (Aspartylglucosaminidase)
(N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase)
(AGA) [Contains: Glycosylasparaginase alpha chain;
Glycosylasparaginase beta chain]
Length = 363
Score = 118 bits (296), Expect = 2e-26
Identities = 65/154 (42%), Positives = 92/154 (59%), Gaps = 1/154 (0%)
Frame = +3
Query: 336 NDNNRVAGTVDEHHHDTIGMIALNRNGSMAVGTSTNGAIFKVPGRVGDTAIPGSGGYVDS 515
N ++ V++ +HDTIGM+ + + GTS+NGA FK+PGRVGD+ IPG+G Y +
Sbjct: 196 NQSDEAGYLVEKTNHDTIGMVVRDTENIFSAGTSSNGARFKIPGRVGDSPIPGAGAYANK 255
Query: 516 TIGGAVGTGDGDVLMRFLLSYQAVEFMRQGMHPNKACVESLRRV-KQPGVWYGALLAMNN 692
GGA TGDGDV+MRFL S+ AV M G P+KA +++ R+ K + GA++AMN
Sbjct: 256 -FGGAAATGDGDVMMRFLPSFFAVTQMELGTKPSKAAYKAITRILKVFPKFSGAVVAMNV 314
Query: 693 AGEYGAACVGFTNYYISIRNAKTRNTTVTTKIDC 794
G GA+C + ++ A T VT I C
Sbjct: 315 KGRIGASCANINKFGYNV--AFQNGTVVTYSISC 346
Score = 30.8 bits (68), Expect = 5.3
Identities = 13/24 (54%), Positives = 19/24 (79%), Gaps = 2/24 (8%)
Frame = +3
Query: 3 FPEDVSPDPTKHCGPYK--PLSRN 68
F ++VSPDP+ CGPYK PL+++
Sbjct: 165 FWKNVSPDPSSSCGPYKTNPLTKS 188
>sp|Q47898|ASPG_FLAME N(4)-(Beta-N-acetylglucosaminyl)-L-asparaginase precursor
(Glycosylasparaginase) (Aspartylglucosaminidase)
(N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase)
(AGA) [Contains: Glycosylasparaginase alpha chain;
Glycosylasparaginase beta chain]
Length = 340
Score = 95.1 bits (235), Expect = 2e-19
Identities = 50/127 (39%), Positives = 78/127 (61%), Gaps = 8/127 (6%)
Frame = +3
Query: 363 VDEHHHDTIGMIALNRNGSMAVGTSTNGAIFKVPGRVGDTAIPGSGGYVDSTIGGAVGTG 542
V+ +HDTIGMIAL+ G+++ +T+G +K+ GRVGD+ I G+G +VD+ IG A TG
Sbjct: 190 VNIENHDTIGMIALDAQGNLSGACTTSGMAYKMHGRVGDSPIIGAGLFVDNEIGAATATG 249
Query: 543 DGDVLMRFLLSYQAVEFMRQGMHPNKACVESLRRVKQPGVWYG--------ALLAMNNAG 698
G+ ++R + ++ VE M QG P +AC E++ R+ + G +A+N G
Sbjct: 250 HGEEVIRTVGTHLVVELMNQGRTPQQACKEAVERIVKIVNRRGKNLKDIQVGFIALNKKG 309
Query: 699 EYGAACV 719
EYGA C+
Sbjct: 310 EYGAYCI 316
>sp|P37595|ASGX_ECOLI Putative L-asparaginase precursor (L-asparagine amidohydrolase)
Length = 321
Score = 63.2 bits (152), Expect = 1e-09
Identities = 39/134 (29%), Positives = 68/134 (50%), Gaps = 2/134 (1%)
Frame = +3
Query: 303 DEIISTGKDATNDNNRVAGTVDEHHH-DTIGMIALNRNGSMAVGTSTNGAIFKVPGRVGD 479
+++++ K+ + +DE T+G +AL+ +G++A TST G K+PGRVGD
Sbjct: 151 EQLLAARKEGATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGD 210
Query: 480 TAIPGSGGYVDSTIGGAVGTGDGDVLMRFLLSYQAVEFMRQGMHPNKACVESLRRVKQPG 659
+ + G+G Y ++ TG G+V +R L +Y M G E + K P
Sbjct: 211 SPLVGAGCYANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPA 270
Query: 660 V-WYGALLAMNNAG 698
+ G L+A+++ G
Sbjct: 271 LGGSGGLIAIDHEG 284
>sp|O57971|ASGX_PYRHO Putative L-asparaginase (L-asparagine amidohydrolase)
Length = 305
Score = 57.0 bits (136), Expect = 7e-08
Identities = 34/80 (42%), Positives = 47/80 (58%)
Frame = +3
Query: 384 TIGMIALNRNGSMAVGTSTNGAIFKVPGRVGDTAIPGSGGYVDSTIGGAVGTGDGDVLMR 563
T+G +A + +A GTST G K+ GRVGDT I G+G Y + + GA TG G+V +R
Sbjct: 175 TVGAVAFDGEEIVA-GTSTGGVFLKMFGRVGDTPIIGAGTYANE-VAGASCTGLGEVAIR 232
Query: 564 FLLSYQAVEFMRQGMHPNKA 623
L+ A +F+R GM A
Sbjct: 233 LALAKTATDFVRLGMDAQAA 252
>sp|Q5JHT1|ASGX_PYRKO Putative L-asparaginase (L-asparagine amidohydrolase)
Length = 306
Score = 55.8 bits (133), Expect = 2e-07
Identities = 33/80 (41%), Positives = 46/80 (57%)
Frame = +3
Query: 384 TIGMIALNRNGSMAVGTSTNGAIFKVPGRVGDTAIPGSGGYVDSTIGGAVGTGDGDVLMR 563
T+G +A + +A GTST G K+ GRVGDT I G G Y + + GA TG G+V ++
Sbjct: 176 TVGAVAFDGEEVVA-GTSTGGVFLKMFGRVGDTPIIGGGTYANE-VAGASCTGLGEVAIK 233
Query: 564 FLLSYQAVEFMRQGMHPNKA 623
L+ A +F+R GM A
Sbjct: 234 LALAKSAADFVRLGMDAQTA 253
>sp|Q8U4E6|ASGX_PYRFU Putative L-asparaginase (L-asparagine amidohydrolase)
Length = 306
Score = 55.8 bits (133), Expect = 2e-07
Identities = 33/86 (38%), Positives = 50/86 (58%)
Frame = +3
Query: 384 TIGMIALNRNGSMAVGTSTNGAIFKVPGRVGDTAIPGSGGYVDSTIGGAVGTGDGDVLMR 563
T+G +A + +A GTST G K+ GRVGDT I G+G Y + + GA TG G+V ++
Sbjct: 176 TVGAVAFDGEEIVA-GTSTGGVFLKMFGRVGDTPIIGAGTYANE-VAGASCTGLGEVAIK 233
Query: 564 FLLSYQAVEFMRQGMHPNKACVESLR 641
L+ A +F+R G+ A ++R
Sbjct: 234 LSLAKTATDFVRLGLDAQAASEAAIR 259
>sp|Q9V262|ASGX_PYRAB Putative L-asparaginase (L-asparagine amidohydrolase)
Length = 305
Score = 54.7 bits (130), Expect = 3e-07
Identities = 32/80 (40%), Positives = 47/80 (58%)
Frame = +3
Query: 384 TIGMIALNRNGSMAVGTSTNGAIFKVPGRVGDTAIPGSGGYVDSTIGGAVGTGDGDVLMR 563
T+G +A + +A GTST G K+ GRVGDT I G+G Y + + GA TG G+V ++
Sbjct: 175 TVGAVAFDGEEVVA-GTSTGGVFLKMFGRVGDTPIIGAGTYANE-VAGASCTGLGEVAIK 232
Query: 564 FLLSYQAVEFMRQGMHPNKA 623
L+ A +F+R G+ A
Sbjct: 233 LALAKTATDFVRLGLDAQAA 252
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 103,884,856
Number of Sequences: 369166
Number of extensions: 2157196
Number of successful extensions: 8482
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6720
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 8163
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8886314050
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)